################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI Alw44I AlwNI AocI ApaLI ApoI AscI AseI AsnI Asp718I AspI AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MflI MluI MroI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuII RcaI RsrII SacII SalI SbfI SfiI SfuI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tsp509I Tth111I Van91I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 685 GTCTAC GT^[AC][GT]AC AhdI 760 GACGGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1133 AGCGCT AGC^GCT AspEI 760 GACGGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 716 TGCGCA TGC^GCA BlpI 729 GCTCAGC GC^T[ACGT]AGC BpmI 1005 CTCCAG CTGGAG(16/14) Bpu1102I 729 GCTCAGC GC^T[ACGT]AGC BseRI 1365 GAGGAG GAGGAG(10/8) BspMI 360 GCAGGT ACCTGC(4/8) BsrFI 1076 GCCGGC [GA]^CCGG[CT] BstAPI 1094 GCAGCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 729 GCTCAGC GC^T[ACGT]AGC Cfr10I 1076 GCCGGC [GA]^CCGG[CT] Eam1105I 760 GACGGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1133 AGCGCT AGC^GCT FspI 716 TGCGCA TGC^GCA HinfI 985 GAGTC G^A[ACGT]TC MaeIII 457 GTGAC ^GT[ACGT]AC MseI 1343 TTAA T^TAA NaeI 1076 GCCGGC GCC^GGC NgoMIV 1076 GCCGGC G^CCGGC PleI 985 GAGTC GAGTC(4/5) PpuMI 1009 AGGTCCC [GA]G^G[AT]CC[CT] PvuI 413 CGATCG CGAT^CG SacI 334 GAGCTC GAGCT^C SapI 1251 GCTCTTC GCTCTTC(1/4) ScaI 134 AGTACT AGT^ACT SexAI 622 ACCTGGT A^CC[AT]GGT SfcI 1203 CTACAG C^T[GA][CT]AG SgrAI 1075 CGCCGGCG C[GA]^CCGG[CT]G SmaI 1014 CCCGGG CCC^GGG StuI 1229 AGGCCT AGG^CCT Tru9I 1343 TTAA T^TAA Tsp45I 457 GTGAC ^GT[GC]AC XcmI 329 CCAACGAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1014 CCCGGG C^CCGGG XmaI 1014 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwI 1337 GATCC GGATC(4/5) 1271 GGATC Asp700I 258 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1131 GAAGCGCTTC AspHI 334 GAGCTC G[AT]GC[AT]^C 1174 GTGCTC AvaI 102 CTCGGG C^[CT]CG[GA]G 1014 CCCGGG AvaII 441 GGACC G^G[AT]CC 1010 GGTCC BbsI 187 GTCTTC GAAGAC(2/6) 1315 GTCTTC BglI 100 GCCTCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1208 GCCTGTACGGC BpuAI 187 GTCTTC GAAGAC(2/6) 1315 GTCTTC BsaI 619 GAGACC GGTCTC(1/5) 561 GGTCTC BsiHKAI 334 GAGCTC G[AT]GC[AT]^C 1174 GTGCTC BsmAI 619 GAGAC GTCTC(1/5) 562 GTCTC BsoBI 102 CTCGGG C^[CT]CG[GA]G 1014 CCCGGG BstXI 431 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 509 CCACCATCGTGG DdeI 730 CTCAG C^T[ACGT]AG 983 CTGAG MaeII 206 ACGT A^CGT 845 ACGT XmnI 258 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1131 GAAGCGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccII AciI AcyI AfaI AluI ApaI BalI BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaJI BsiEI BsiYI BslI BsmFI Bsp1286I BsrBI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KasI Ksp632I MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfoI TaqI TseI TspRI TthHB8I