(Circular) (Six-base) MAPSORT of: pFN21AE1925.seq Check: 3165 from: 1 to: 6091 pFN21AE1925 6091 bp With 182 enzymes: SgfI * March 28, 2012 11:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2356 2356 Size: 6091 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2691 3434 1937 Size: 754 743 4594 Fragments arranged by size: 4594 754 743 Acc65I G'GTAC_C Cuts at: 3412 3412 Size: 6091 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2507 3187 3367 4154 5538 5806 1632 Size: 875 680 180 787 1384 268 1917 Fragments arranged by size: 1917 1384 875 787 680 268 180 AfeI AGC'GCT Cuts at: 3140 3140 Size: 6091 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 4990 5967 1203 Size: 3787 977 1327 Fragments arranged by size: 3787 1327 977 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 2770 4820 2770 Size: 2050 4041 AleI CACnn'nnGTG Cuts at: 1166 2201 2519 3149 1166 Size: 1035 318 630 4108 Fragments arranged by size: 4108 1035 630 318 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2448 1184 Size: 1264 4827 AlwNI CAG_nnn'CTG Cuts at: 3834 5406 3834 Size: 1572 4519 ApaI G_GGCC'C Cuts at: 2057 2762 3027 2057 Size: 705 265 5121 Fragments arranged by size: 5121 705 265 ApaLI G'TGCA_C Cuts at: 4148 5304 4148 Size: 1156 4935 ApoI r'AATT_y Cuts at: 1493 3400 3626 3778 5887 1493 Size: 1907 226 152 2109 1697 Fragments arranged by size: 2109 1907 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4645 160 Size: 872 3613 1606 Fragments arranged by size: 3613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 2107 3019 3416 1870 Size: 73 164 912 397 4545 Fragments arranged by size: 4545 912 397 164 73 BamHI G'GATC_C Cuts at: 3421 3788 3421 Size: 367 5724 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2591 2892 3084 3244 3412 Size: 325 217 627 804 301 192 160 168 Cuts at: 3412 4867 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 804 627 325 301 217 192 168 160 BanII G_rGCy'C Cuts at: 1391 2057 2343 2762 3027 3410 1391 Size: 666 286 419 265 383 4072 Fragments arranged by size: 4072 666 419 383 286 265 BbeI G_GCGC'C Cuts at: 2595 2896 3088 2595 Size: 301 192 5598 Fragments arranged by size: 5598 301 192 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2185 3313 928 Size: 971 286 1128 3706 Fragments arranged by size: 3706 1128 971 286 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4282 4316 1903 Size: 34 2345 34 3678 Fragments arranged by size: 3678 2345 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 1548 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2111 3219 4702 Size: 107 122 71 631 605 439 1108 1483 Cuts at: 4702 136 Size: 1525 Fragments arranged by size: 1525 1483 1108 631 605 439 122 107 71 BglII A'GATC_T Cuts at: 6086 6086 Size: 6091 BlpI GC'TnA_GC Cuts at: 2735 3508 2735 Size: 773 5318 Bme1580I G_kGCm'C Cuts at: 1790 2057 2762 2829 3001 3027 3249 4152 5308 Size: 267 705 67 172 26 222 903 1156 Cuts at: 5308 1790 Size: 2573 Fragments arranged by size: 2573 1156 903 705 267 222 172 67 26 BmgBI CAC'GTC Cuts at: 1318 2851 1318 Size: 1533 4558 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3822 4780 442 Size: 416 53 83 399 24 2405 958 1753 Fragments arranged by size: 2405 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2995 4751 1239 Size: 1756 1756 2579 Fragments arranged by size: 2579 1756 1756 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4216 5081 5379 4216 Size: 865 298 4928 Fragments arranged by size: 4928 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2572 2618 4754 882 Size: 617 1073 46 2136 2219 Fragments arranged by size: 2219 2136 1073 617 46 BsaAI yAC'GTr Cuts at: 493 1204 3882 493 Size: 711 2678 2702 Fragments arranged by size: 2702 2678 711 BsaBI GATnn'nnATC Cuts at: 6085 6085 Size: 6091 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2592 2893 3085 Size: 53 83 186 936 476 583 301 192 Cuts at: 3085 3898 4280 4915 275 Size: 813 382 635 1451 Fragments arranged by size: 1451 936 813 635 583 476 382 301 192 186 83 53 BsaWI w'CCGG_w Cuts at: 4524 4922 5196 5343 5754 4524 Size: 398 274 147 411 4861 Fragments arranged by size: 4861 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5866 Fragments arranged by size: 5866 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3385 1186 Size: 2199 3892 BseYI C'CCAG_C Cuts at: 2794 5294 5790 2794 Size: 2500 496 3095 Fragments arranged by size: 3095 2500 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5518 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2292 2421 2544 2880 3541 4302 Size: 275 186 291 129 123 336 661 761 Cuts at: 4302 5330 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 661 336 291 275 186 129 123 BsiHKAI G_wGCw'C Cuts at: 2343 3183 4152 4237 5308 2343 Size: 840 969 85 1071 3126 Fragments arranged by size: 3126 1071 969 840 85 BsmI GAATG_Cn' Cuts at: 3602 3695 3602 Size: 93 5998 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2343 2762 2829 3001 3027 3183 Size: 399 267 286 419 67 172 26 156 Cuts at: 3183 3249 3410 4152 4237 5308 1391 Size: 66 161 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 419 399 286 267 172 161 156 85 67 66 26 BspEI T'CCGG_A Cuts at: 5754 5754 Size: 6091 BspHI T'CATG_A Cuts at: 3980 5710 3980 Size: 1730 4361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2356 3379 3446 844 Size: 1512 1023 67 3489 Fragments arranged by size: 3489 1512 1023 67 BsrBI CCG'CTC Cuts at: 1394 1823 2067 2930 3256 3978 1394 Size: 429 244 863 326 722 3507 Fragments arranged by size: 3507 863 722 429 326 244 BsrDI GCAATG_nn' Cuts at: 65 1248 4586 4760 65 Size: 1183 3338 174 1396 Fragments arranged by size: 3338 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3081 4735 4922 1700 Size: 110 1271 1654 187 2869 Fragments arranged by size: 2869 1654 1271 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6091 BssHII G'CGCG_C Cuts at: 1925 2251 2253 2696 2746 2832 3043 3092 3128 Size: 326 2 443 50 86 211 49 36 Cuts at: 3128 1925 Size: 4888 Fragments arranged by size: 4888 443 326 211 86 50 49 36 2 BssSI C'ACGA_G Cuts at: 4151 5163 4151 Size: 1012 5079 BstAPI GCAn_nnn'nTGC Cuts at: 794 3105 794 Size: 2311 3780 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6091 BstXI CCAn_nnnn'nTGG Cuts at: 2443 2521 2443 Size: 78 6013 BstYI r'GATC_y Cuts at: 1972 3421 3788 4173 4190 5642 5728 5740 6086 Size: 1449 367 385 17 1452 86 12 346 Cuts at: 6086 1972 Size: 1977 Fragments arranged by size: 1977 1452 1449 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2172 2199 2754 513 Size: 552 939 168 27 555 3850 Fragments arranged by size: 3850 939 555 552 168 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5352 Fragments arranged by size: 5352 683 56 BtsI GCAGTG_nn' Cuts at: 2612 2827 3607 4401 4421 2612 Size: 215 780 794 20 4282 Fragments arranged by size: 4282 794 780 215 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 3395 3395 Size: 6091 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5098 809 Size: 507 3782 1802 Fragments arranged by size: 3782 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2134 2289 2304 Size: 54 1425 50 275 18 304 155 15 Cuts at: 2304 2331 2523 2541 2712 2877 3031 3538 4427 Size: 27 192 18 171 165 154 507 889 Cuts at: 4427 6056 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 507 304 275 192 171 165 155 154 54 50 43 27 18 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2289 2541 2877 3538 1537 Size: 275 477 252 336 661 4090 Fragments arranged by size: 4090 661 477 336 275 252 EarI CTCTTCn'nnn_ Cuts at: 1353 2273 3263 3365 4021 1353 Size: 920 990 102 656 3423 Fragments arranged by size: 3423 990 920 656 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4678 5052 5198 387 Size: 1064 138 3089 374 146 1280 Fragments arranged by size: 3089 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2341 2341 Size: 6091 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2507 2995 3187 3367 4154 4751 5538 Size: 393 875 488 192 180 787 597 787 Cuts at: 5538 5806 1239 Size: 268 1524 Fragments arranged by size: 1524 875 787 787 597 488 393 268 192 180 EcoO109I rG'GnC_Cy Cuts at: 2054 2759 3015 3023 2054 Size: 705 256 8 5122 Fragments arranged by size: 5122 705 256 8 EcoRI G'AATT_C Cuts at: 3400 3778 3400 Size: 378 5713 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3286 3286 Size: 6091 FspI TGC'GCA Cuts at: 2723 4597 2723 Size: 1874 4217 HaeII r_GCGC'y Cuts at: 1678 2021 2033 2249 2393 2595 2896 3088 3142 Size: 343 12 216 144 202 301 192 54 Cuts at: 3142 5238 1678 Size: 2096 2531 Fragments arranged by size: 2531 2096 343 301 216 202 192 144 54 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2410 2442 2756 2788 4732 Size: 32 135 32 838 32 314 32 1944 Cuts at: 4732 4764 4806 4838 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1944 838 314 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3435 3681 669 Size: 988 281 1497 246 3079 Fragments arranged by size: 3079 1497 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6091 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KasI G'GCGC_C Cuts at: 2591 2892 3084 2591 Size: 301 192 5598 Fragments arranged by size: 5598 301 192 KpnI G_GTAC'C Cuts at: 3416 3416 Size: 6091 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3388 5205 5389 1305 Size: 2083 1817 184 2007 Fragments arranged by size: 2083 2007 1817 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2306 2333 2714 10 Size: 54 1425 343 474 27 381 3387 Fragments arranged by size: 3387 1425 474 381 343 54 27 MslI CAynn'nnrTG Cuts at: 518 1166 2201 2300 2430 2519 3149 3170 4049 Size: 648 1035 99 130 89 630 21 879 Cuts at: 4049 4408 4567 518 Size: 359 159 2042 Fragments arranged by size: 2042 1035 879 648 630 359 159 130 99 89 21 MspA1I CmG'CkG Cuts at: 1606 2048 2088 2563 2876 3507 4184 5332 5577 Size: 442 40 475 313 631 677 1148 245 Cuts at: 5577 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 631 475 442 313 245 40 NaeI GCC'GGC Cuts at: 3083 3083 Size: 6091 NarI GG'CG_CC Cuts at: 2592 2893 3085 2592 Size: 301 192 5598 Fragments arranged by size: 5598 301 192 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4600 Fragments arranged by size: 4600 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NgoMIV G'CCGG_C Cuts at: 3081 3081 Size: 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 3449 4994 1132 Size: 97 2220 1545 2229 Fragments arranged by size: 2229 2220 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6091 PciI A'CATG_T Cuts at: 4990 4990 Size: 6091 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6091 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3015 3015 Size: 6091 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6091 PsiI TTA'TAA Cuts at: 3661 3661 Size: 6091 PspOMI G'GGCC_C Cuts at: 2053 2758 3023 2053 Size: 705 265 5121 Fragments arranged by size: 5121 705 265 PstI C_TGCA'G Cuts at: 830 3443 830 Size: 2613 3478 PvuI CG_AT'CG Cuts at: 2001 2421 2001 Size: 420 5671 SacI G_AGCT'C Cuts at: 2343 2343 Size: 6091 SalI G'TCGA_C Cuts at: 1936 3433 1936 Size: 1497 4594 SapI GCTCTTCn'nnn_ Cuts at: 3263 3263 Size: 6091 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 2141 4339 6051 2141 Size: 2198 1712 2181 Fragments arranged by size: 2198 2181 1712 SexAI A'CCwGG_T Cuts at: 2627 2627 Size: 6091 SfcI C'TryA_G Cuts at: 826 1045 3208 3439 4574 5255 5446 826 Size: 219 2163 231 1135 681 191 1471 Fragments arranged by size: 2163 1471 1135 681 231 219 191 SfoI GGC'GCC Cuts at: 2593 2894 3086 2593 Size: 301 192 5598 Fragments arranged by size: 5598 301 192 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 3081 1700 Size: 1381 4710 SmaI CCC'GGG Cuts at: 1872 3021 3418 1872 Size: 1149 397 4545 Fragments arranged by size: 4545 1149 397 SmlI C'TyrA_G Cuts at: 820 1017 1943 4195 5096 5358 820 Size: 197 926 2252 901 262 1553 Fragments arranged by size: 2252 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 3449 1132 Size: 2317 3774 SspI AAT'ATT Cuts at: 5 52 4015 5 Size: 47 3963 2081 Fragments arranged by size: 3963 2081 47 StuI AGG'CCT Cuts at: 1517 3236 1517 Size: 1719 4372 StyI C'CwwG_G Cuts at: 513 1065 2004 3530 513 Size: 552 939 1526 3074 Fragments arranged by size: 3074 1526 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4118 4135 4288 4473 4918 325 Size: 1255 2538 17 153 185 445 1498 Fragments arranged by size: 2538 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2139 2478 4337 Size: 274 80 33 51 1443 162 339 1859 Cuts at: 4337 6049 96 Size: 1712 138 Fragments arranged by size: 1859 1712 1443 339 274 162 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6051 6083 6051 Size: 32 6059 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6091 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2341 2341 Size: 6091 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 3019 3416 1870 Size: 1149 397 4545 Fragments arranged by size: 4545 1149 397 XmnI GAAnn'nnTTC Cuts at: 2267 3140 4220 2267 Size: 873 1080 4138 Fragments arranged by size: 4138 1080 873 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI SfiI SrfI SwaI