(Linear) (Six-base) MAPSORT of: KIEE1925.seq Check: 3573 from: 1 to: 1386 KIEE1925.orf With 182 enzymes: SgfI * March 22, 2012 15:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 351 1386 Size: 351 1035 AccI GT'mk_AC Cuts at: 0 686 1386 Size: 686 700 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 502 1182 1362 1386 Size: 502 680 180 24 Fragments arranged by size: 680 502 180 24 AfeI AGC'GCT Cuts at: 0 1135 1386 Size: 1135 251 AhdI GACnn_n'nnGTC Cuts at: 0 765 1386 Size: 765 621 AleI CACnn'nnGTG Cuts at: 0 196 514 1144 1386 Size: 196 318 630 242 Fragments arranged by size: 630 318 242 196 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 443 1386 Size: 443 943 ApaI G_GGCC'C Cuts at: 0 52 757 1022 1386 Size: 52 705 265 364 Fragments arranged by size: 705 364 265 52 AvaI C'yCGr_G Cuts at: 0 102 1014 1386 Size: 102 912 372 Fragments arranged by size: 912 372 102 BanI G'GyrC_C Cuts at: 0 586 887 1079 1239 1386 Size: 586 301 192 160 147 Fragments arranged by size: 586 301 192 160 147 BanII G_rGCy'C Cuts at: 0 52 338 757 1022 1386 Size: 52 286 419 265 364 Fragments arranged by size: 419 364 286 265 52 BbeI G_GCGC'C Cuts at: 0 590 891 1083 1386 Size: 590 301 192 303 Fragments arranged by size: 590 303 301 192 BbsI GAAGACnn'nnnn_ Cuts at: 0 180 1308 1386 Size: 180 1128 78 Fragments arranged by size: 1128 180 78 BglI GCCn_nnn'nGGC Cuts at: 0 106 1214 1386 Size: 106 1108 172 Fragments arranged by size: 1108 172 106 BlpI GC'TnA_GC Cuts at: 0 730 1386 Size: 730 656 Bme1580I G_kGCm'C Cuts at: 0 52 757 824 996 1022 1244 1386 Size: 52 705 67 172 26 222 142 Fragments arranged by size: 705 222 172 142 67 52 26 BmgBI CAC'GTC Cuts at: 0 846 1386 Size: 846 540 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 990 1386 Size: 990 396 BsaI GGTCTCn'nnnn_ Cuts at: 0 567 613 1386 Size: 567 46 773 Fragments arranged by size: 773 567 46 BsaHI Gr'CG_yC Cuts at: 0 4 587 888 1080 1386 Size: 4 583 301 192 306 Fragments arranged by size: 583 306 301 192 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1380 1386 Size: 1380 6 BseYI C'CCAG_C Cuts at: 0 789 1386 Size: 789 597 BsiEI CG_ry'CG Cuts at: 0 287 416 539 875 1386 Size: 287 129 123 336 511 Fragments arranged by size: 511 336 287 129 123 BsiHKAI G_wGCw'C Cuts at: 0 338 1178 1386 Size: 338 840 208 Fragments arranged by size: 840 338 208 Bsp1286I G_dGCh'C Cuts at: 0 52 338 757 824 996 1022 1178 1244 Size: 52 286 419 67 172 26 156 66 Cuts at: 1244 1386 Size: 142 Fragments arranged by size: 419 286 172 156 142 67 66 52 26 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 351 1386 Size: 351 1035 BsrBI CCG'CTC Cuts at: 0 62 925 1251 1386 Size: 62 863 326 135 Fragments arranged by size: 863 326 135 62 BsrFI r'CCGG_y Cuts at: 0 1076 1386 Size: 1076 310 BssHII G'CGCG_C Cuts at: 0 246 248 691 741 827 1038 1087 1123 Size: 246 2 443 50 86 211 49 36 Cuts at: 1123 1386 Size: 263 Fragments arranged by size: 443 263 246 211 86 50 49 36 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 1100 1386 Size: 1100 286 BstXI CCAn_nnnn'nTGG Cuts at: 0 438 516 1386 Size: 438 78 870 Fragments arranged by size: 870 438 78 BtgI C'CryG_G Cuts at: 0 167 194 749 1386 Size: 167 27 555 637 Fragments arranged by size: 637 555 167 27 BtsI GCAGTG_nn' Cuts at: 0 607 822 1386 Size: 607 215 564 Fragments arranged by size: 607 564 215 EaeI y'GGCC_r Cuts at: 0 129 284 299 326 518 536 707 872 Size: 129 155 15 27 192 18 171 165 Cuts at: 872 1026 1386 Size: 154 360 Fragments arranged by size: 360 192 171 165 155 154 129 27 18 15 EagI C'GGCC_G Cuts at: 0 284 536 872 1386 Size: 284 252 336 514 Fragments arranged by size: 514 336 284 252 EarI CTCTTCn'nnn_ Cuts at: 0 268 1258 1360 1386 Size: 268 990 102 26 Fragments arranged by size: 990 268 102 26 EcoICRI GAG'CTC Cuts at: 0 336 1386 Size: 336 1050 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 502 990 1182 1362 1386 Size: 502 488 192 180 24 Fragments arranged by size: 502 488 192 180 24 EcoO109I rG'GnC_Cy Cuts at: 0 49 754 1010 1018 1386 Size: 49 705 256 8 368 Fragments arranged by size: 705 368 256 49 8 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1281 1386 Size: 1281 105 FspI TGC'GCA Cuts at: 0 718 1386 Size: 718 668 HaeII r_GCGC'y Cuts at: 0 16 28 244 388 590 891 1083 1137 Size: 16 12 216 144 202 301 192 54 Cuts at: 1137 1386 Size: 249 Fragments arranged by size: 301 249 216 202 192 144 54 16 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 405 437 751 783 1386 Size: 405 32 314 32 603 Fragments arranged by size: 603 405 314 32 32 KasI G'GCGC_C Cuts at: 0 586 887 1079 1386 Size: 586 301 192 307 Fragments arranged by size: 586 307 301 192 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1383 1386 Size: 1383 3 MscI TGG'CCA Cuts at: 0 301 328 709 1386 Size: 301 27 381 677 Fragments arranged by size: 677 381 301 27 MslI CAynn'nnrTG Cuts at: 0 196 295 425 514 1144 1165 1386 Size: 196 99 130 89 630 21 221 Fragments arranged by size: 630 221 196 130 99 89 21 MspA1I CmG'CkG Cuts at: 0 43 83 558 871 1386 Size: 43 40 475 313 515 Fragments arranged by size: 515 475 313 43 40 NaeI GCC'GGC Cuts at: 0 1078 1386 Size: 1078 308 NarI GG'CG_CC Cuts at: 0 587 888 1080 1386 Size: 587 301 192 306 Fragments arranged by size: 587 306 301 192 NgoMIV G'CCGG_C Cuts at: 0 1076 1386 Size: 1076 310 PpuMI rG'GwC_Cy Cuts at: 0 1010 1386 Size: 1010 376 PspOMI G'GGCC_C Cuts at: 0 48 753 1018 1386 Size: 48 705 265 368 Fragments arranged by size: 705 368 265 48 PvuI CG_AT'CG Cuts at: 0 416 1386 Size: 416 970 SacI G_AGCT'C Cuts at: 0 338 1386 Size: 338 1048 SapI GCTCTTCn'nnn_ Cuts at: 0 1258 1386 Size: 1258 128 ScaI AGT'ACT Cuts at: 0 136 1386 Size: 136 1250 SexAI A'CCwGG_T Cuts at: 0 622 1386 Size: 622 764 SfcI C'TryA_G Cuts at: 0 1203 1386 Size: 1203 183 SfoI GGC'GCC Cuts at: 0 588 889 1081 1386 Size: 588 301 192 305 Fragments arranged by size: 588 305 301 192 SgrAI Cr'CCGG_yG Cuts at: 0 1076 1386 Size: 1076 310 SmaI CCC'GGG Cuts at: 0 1016 1386 Size: 1016 370 StuI AGG'CCT Cuts at: 0 1231 1386 Size: 1231 155 TatI w'GTAC_w Cuts at: 0 134 473 1386 Size: 134 339 913 Fragments arranged by size: 913 339 134 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 336 1386 Size: 336 1050 XmaI C'CCGG_G Cuts at: 0 1014 1386 Size: 1014 372 XmnI GAAnn'nnTTC Cuts at: 0 262 1135 1386 Size: 262 873 251 Fragments arranged by size: 873 262 251 Enzymes that do cut: AarI AccI AcuI AfeI AhdI AleI AlfI ApaI AvaI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI BpmI BsaI BsaHI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstXI BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV PpuMI PspOMI PvuI SacI SapI ScaI SexAI SfcI SfoI SgrAI SmaI StuI TatI XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EciI EcoNI EcoRI EcoRV FseI FspAI HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuII RsrII SacII SalI SanDI SbfI SfiI SgfI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII TstI Tth111I XbaI XhoI ZraI