Restriction map for
pFN21AE3113
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI AscI AseI AsnI
AspEI AspI AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV FbaI FseI
HgaI HincII HindII HindIII HpaI
HphI MaeI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MunI NcoI NdeI NdeII NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SalI SapI Sau3AI SbfI
ScaI SexAI SfaNI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 197 GGTACC G^GTACC
AccI 367 GTCTAC GT^[AC][GT]AC
AcsI 686 GAATTC [GA]^AATT[CT]
AcyI 6 GGCGCC G[GA]^CG[CT]C
AfaI 198 GTAC GT^AC
AlwNI 794 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 686 GAATTC [GA]^AATT[CT]
Asp700I 682 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 197 GGTACC G^GTACC
AspHI 417 GAGCAC G[AT]GC[AT]^C
AvaII 434 GGACC G^G[AT]CC
AviII 275 TGCGCA TGC^GCA
BbeI 6 GGCGCC GGCGC^C
BsaAI 320 TACGTG [CT]AC^GT[GA]
BsaHI 6 GGCGCC G[GA]^CG[CT]C
BsgI 208 CTGCAC GTGCAG(16/14)
BsiHKAI 417 GAGCAC G[AT]GC[AT]^C
BspMI 296 ACCTGC ACCTGC(4/8)
BsrI 194 ACTGG ACTGG(1/-1)
BstAPI 793 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 285 CATCC GGATG(2/0)
DdeI 413 CTCAG C^T[ACGT]AG
DsaI 95 CCGCGG C^C[GA][CT]GG
EarI 265 GAAGAG CTCTTC(1/4)
EcoRI 686 GAATTC G^AATTC
EcoT22I 464 ATGCAT ATGCA^T
FokI 285 CATCC GGATG(9/13)
FspI 275 TGCGCA TGC^GCA
Hin1I 6 GGCGCC G[GA]^CG[CT]C
KasI 6 GGCGCC G^GCGCC
KpnI 197 GGTACC GGTAC^C
Ksp632I 265 GAAGAG CTCTTC(1/4)
KspI 95 CCGCGG CCGC^GG
MaeIII 823 GTGAC ^GT[ACGT]AC
MseI 374 TTAA T^TAA
NarI 6 GGCGCC GG^CGCC
NsiI 464 ATGCAT ATGCA^T
NspI 333 ACATGC [GA]CATG^[CT]
PleI 692 GAGTC GAGTC(4/5)
RsaI 198 GTAC GT^AC
SacII 95 CCGCGG CCGC^GG
SfcI 48 CTGTAG C^T[GA][CT]AG
SfiI 93 GGCCGCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 6 GGCGCC GGC^GCC
SmaI 15 CCCGGG CCC^GGG
TfiI 594 GAATC G^A[AT]TC
Tru9I 374 TTAA T^TAA
Tsp45I 823 GTGAC ^GT[GC]AC
XcmI 119 CCAGCCGGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 15 CCCGGG C^CCGGG
XmaI 15 CCCGGG C^CCGGG
XmnI 682 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
ApaI 486 GGGCCC GGGCC^C
530 GGGCCC
BanI 6 GGCGCC G^G[CT][GA]CC
197 GGTACC
BsrDI 408 CATTGC GCAATG(2/0)
560 CATTGC
BsrFI 9 GCCGGC [GA]^CCGG[CT]
110 GCCGGC
Cfr10I 9 GCCGGC [GA]^CCGG[CT]
110 GCCGGC
EcoT14I 546 CCAAGG C^C[AT][AT]GG
837 CCTTGG
HaeII 6 GGCGCC [GA]GCGC^[CT]
40 GGCGCT
HinfI 594 GAATC G^A[ACGT]TC
692 GAGTC
MaeII 321 ACGT A^CGT
732 ACGT
MslI 211 CACGGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
816 CACAGAAGTG
MspA1I 95 CCGCGG C[AC]G^C[GT]G
201 CCGCTG
NaeI 9 GCCGGC GCC^GGC
110 GCCGGC
NgoMIV 9 GCCGGC G^CCGGC
110 GCCGGC
StyI 546 CCAAGG C^C[AT][AT]GG
837 CCTTGG
TaqI 399 TCGA T^CGA
690 TCGA
Tsp509I 516 AATT ^AATT
687 AATT
TthHB8I 399 TCGA T^CGA
690 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI AvaI BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BsiYI BslI BsoBI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DraII EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MboII MnlI MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV Sau96I ScrFI TseI TspRI