(Circular) (Six-base) MAPSORT of: pFN21AE3113.seq Check: 9833 from: 1 to: 5563 pFN21AE3113 5563 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3373 278 Size: 53 83 186 936 1837 2468 Fragments arranged by size: 2468 1837 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2373 2906 1937 Size: 436 533 4594 Fragments arranged by size: 4594 533 436 Acc65I G'GTAC_C Cuts at: 2202 2884 2202 Size: 682 4881 AclI AA'CG_TT Cuts at: 1563 3690 4063 1563 Size: 2127 373 3063 Fragments arranged by size: 3063 2127 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2329 3626 5010 5278 1632 Size: 697 1297 1384 268 1917 Fragments arranged by size: 1917 1384 1297 697 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5366 AflIII A'CryG_T Cuts at: 1203 4462 5439 1203 Size: 3259 977 1327 Fragments arranged by size: 3259 1327 977 AgeI A'CCGG_T Cuts at: 4394 4394 Size: 5563 AhdI GACnn_n'nnGTC Cuts at: 4292 4292 Size: 5563 AleI CACnn'nnGTG Cuts at: 1166 2825 1166 Size: 1659 3904 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5563 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2129 2161 2129 Size: 32 5531 AlwNI CAG_nnn'CTG Cuts at: 2804 3306 4878 2804 Size: 502 1572 3489 Fragments arranged by size: 3489 1572 502 ApaI G_GGCC'C Cuts at: 2495 2539 2495 Size: 44 5519 ApaLI G'TGCA_C Cuts at: 3620 4776 3620 Size: 1156 4407 ApoI r'AATT_y Cuts at: 1493 2691 2872 3098 3250 5359 1493 Size: 1198 181 226 152 2109 1697 Fragments arranged by size: 2109 1697 1198 226 181 152 AseI AT'TA_AT Cuts at: 160 1032 4117 160 Size: 872 3085 1606 Fragments arranged by size: 3085 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5563 AvaI C'yCGr_G Cuts at: 1870 1943 2020 2038 2086 2888 1870 Size: 73 77 18 48 802 4545 Fragments arranged by size: 4545 802 77 73 48 18 BamHI G'GATC_C Cuts at: 2893 3260 2893 Size: 367 5196 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2202 2884 4339 618 Size: 325 217 627 224 191 682 1455 1842 Fragments arranged by size: 1842 1455 682 627 325 224 217 191 BanII G_rGCy'C Cuts at: 1391 2087 2093 2495 2539 2882 1391 Size: 696 6 402 44 343 4072 Fragments arranged by size: 4072 696 402 343 44 6 BbeI G_GCGC'C Cuts at: 2015 2015 Size: 5563 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4592 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3754 3788 1903 Size: 34 1817 34 3678 Fragments arranged by size: 3678 1817 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3455 4665 3455 Size: 1210 4353 BclI T'GATC_A Cuts at: 1548 1548 Size: 5563 BfrBI ATG'CAT Cuts at: 2471 2471 Size: 5563 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2024 2105 Size: 107 122 71 631 605 337 15 81 Cuts at: 2105 2217 2499 4174 136 Size: 112 282 1675 1525 Fragments arranged by size: 1675 1525 631 605 337 282 122 112 107 81 71 15 BglII A'GATC_T Cuts at: 5558 5558 Size: 5563 BlpI GC'TnA_GC Cuts at: 2980 2980 Size: 5563 Bme1580I G_kGCm'C Cuts at: 1790 2495 2504 2539 3624 4780 1790 Size: 705 9 35 1085 1156 2573 Fragments arranged by size: 2573 1156 1085 705 35 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5563 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3294 4252 442 Size: 416 53 83 399 24 1877 958 1753 Fragments arranged by size: 1877 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5563 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2177 2347 4223 1239 Size: 917 21 170 1876 2579 Fragments arranged by size: 2579 1876 917 170 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3688 4553 4851 3688 Size: 865 298 4400 Fragments arranged by size: 4400 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4226 882 Size: 617 2727 2219 Fragments arranged by size: 2727 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2327 3354 493 Size: 711 1123 1027 2702 Fragments arranged by size: 2702 1123 1027 711 BsaBI GATnn'nnATC Cuts at: 5557 5557 Size: 5563 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 3370 3752 4387 Size: 53 83 186 936 479 1358 382 635 Cuts at: 4387 275 Size: 1451 Fragments arranged by size: 1451 1358 936 635 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3996 4394 4668 4815 5226 3996 Size: 398 274 147 411 4333 Fragments arranged by size: 4333 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2129 2159 1178 Size: 30 165 30 726 30 4582 Fragments arranged by size: 4582 726 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5563 BseYI C'CCAG_C Cuts at: 2027 2123 2306 2569 2589 2794 4766 5262 2027 Size: 96 183 263 20 205 1972 496 2328 Fragments arranged by size: 2328 1972 496 263 205 183 96 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 1163 Size: 573 462 4528 Fragments arranged by size: 4528 573 462 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3013 3774 4802 1540 Size: 275 186 1012 761 1028 2301 Fragments arranged by size: 2301 1028 1012 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2426 3624 3709 4780 2426 Size: 1198 85 1071 3209 Fragments arranged by size: 3209 1198 1071 85 BsmI GAATG_Cn' Cuts at: 3074 3167 3074 Size: 93 5470 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2093 2426 2495 2504 2539 2882 Size: 399 297 6 333 69 9 35 343 Cuts at: 2882 3624 3709 4780 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 343 333 297 85 69 35 9 6 BspEI T'CCGG_A Cuts at: 5226 5226 Size: 5563 BspHI T'CATG_A Cuts at: 3452 5182 3452 Size: 1730 3833 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2310 2918 844 Size: 1466 608 3489 Fragments arranged by size: 3489 1466 608 BsrBI CCG'CTC Cuts at: 1394 1823 3450 1394 Size: 429 1627 3507 Fragments arranged by size: 3507 1627 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2412 2564 4058 4232 65 Size: 1183 1164 152 1494 174 1396 Fragments arranged by size: 1494 1396 1183 1164 174 152 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 2115 4207 4394 1700 Size: 110 204 101 2092 187 2869 Fragments arranged by size: 2869 2092 204 187 110 101 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5563 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5563 BssSI C'ACGA_G Cuts at: 3623 4635 3623 Size: 1012 4551 BstAPI GCAn_nnn'nTGC Cuts at: 794 2804 794 Size: 2010 3553 BstBI TT'CG_AA Cuts at: 4367 4367 Size: 5563 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5563 BstYI r'GATC_y Cuts at: 1972 2893 3260 3645 3662 5114 5200 5212 5558 Size: 921 367 385 17 1452 86 12 346 Cuts at: 5558 1972 Size: 1977 Fragments arranged by size: 1977 1452 921 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2100 513 Size: 552 939 96 3976 Fragments arranged by size: 3976 939 552 96 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2235 487 Size: 683 56 1009 3815 Fragments arranged by size: 3815 1009 683 56 BtsI GCAGTG_nn' Cuts at: 2517 2552 2581 3079 3873 3893 2517 Size: 35 29 498 794 20 4187 Fragments arranged by size: 4187 794 498 35 29 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5563 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5528 DraI TTT'AAA Cuts at: 2867 2867 Size: 5563 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4570 809 Size: 507 3254 1802 Fragments arranged by size: 3254 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3010 3899 5528 Size: 54 1425 50 275 18 1180 889 1629 Cuts at: 5528 8 Size: 43 Fragments arranged by size: 1629 1425 1180 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3010 1537 Size: 275 1198 4090 Fragments arranged by size: 4090 1198 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2265 3493 1353 Size: 912 1228 3423 Fragments arranged by size: 3423 1228 912 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2257 4150 4524 4670 387 Size: 1064 138 668 1893 374 146 1280 Fragments arranged by size: 1893 1280 1064 668 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2177 2329 2347 3626 4223 5010 Size: 393 524 21 152 18 1279 597 787 Cuts at: 5010 5278 1239 Size: 268 1524 Fragments arranged by size: 1524 1279 787 597 524 393 268 152 21 18 EcoO109I rG'GnC_Cy Cuts at: 2491 2492 2536 2491 Size: 1 44 5518 Fragments arranged by size: 5518 44 1 EcoRI G'AATT_C Cuts at: 2691 2872 3250 2691 Size: 181 378 5004 Fragments arranged by size: 5004 378 181 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2407 2803 2407 Size: 396 5167 FspI TGC'GCA Cuts at: 2282 4069 2282 Size: 1787 3776 HaeII r_GCGC'y Cuts at: 1678 2015 2049 4710 1678 Size: 337 34 2661 2531 Fragments arranged by size: 2661 2531 337 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4204 4236 4278 4310 1373 Size: 32 135 32 2632 32 42 32 2626 Fragments arranged by size: 2632 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2907 3153 669 Size: 988 281 969 246 3079 Fragments arranged by size: 3079 988 969 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5563 HpaI GTT'AAC Cuts at: 3153 3153 Size: 5563 KasI G'GCGC_C Cuts at: 2011 2011 Size: 5563 KpnI G_GTAC'C Cuts at: 2206 2888 2206 Size: 682 4881 MfeI C'AATT_G Cuts at: 3162 3162 Size: 5563 MluI A'CGCG_T Cuts at: 5439 5439 Size: 5563 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4677 4861 1305 Size: 3372 184 2007 Fragments arranged by size: 3372 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3741 Fragments arranged by size: 3741 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2220 2825 3521 3880 4039 518 Size: 648 1054 605 696 359 159 2042 Fragments arranged by size: 2042 1054 696 648 605 359 159 MspA1I CmG'CkG Cuts at: 1606 2102 2208 2979 3656 4804 5049 1606 Size: 496 106 771 677 1148 245 2120 Fragments arranged by size: 2120 1148 771 677 496 245 106 NaeI GCC'GGC Cuts at: 2016 2117 2016 Size: 101 5462 NarI GG'CG_CC Cuts at: 2012 2012 Size: 5563 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4072 Fragments arranged by size: 4072 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5563 NgoMIV G'CCGG_C Cuts at: 2014 2115 2014 Size: 101 5462 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5563 NotI GC'GGCC_GC Cuts at: 3010 3010 Size: 5563 NsiI A_TGCA'T Cuts at: 2473 2473 Size: 5563 NspI r_CATG'y Cuts at: 1132 1229 2342 2921 4466 1132 Size: 97 1113 579 1545 2229 Fragments arranged by size: 2229 1545 1113 579 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5563 PciI A'CATG_T Cuts at: 4462 4462 Size: 5563 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5563 PfoI T'CCnGG_A Cuts at: 2363 2363 Size: 5563 PmeI GTTT'AAAC Cuts at: 2867 2867 Size: 5563 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2129 2161 3632 3664 5145 5177 2129 Size: 32 1471 32 1481 32 2515 Fragments arranged by size: 2515 1481 1471 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5563 PsiI TTA'TAA Cuts at: 3133 3133 Size: 5563 PspOMI G'GGCC_C Cuts at: 2491 2535 2491 Size: 44 5519 PstI C_TGCA'G Cuts at: 830 2915 830 Size: 2085 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5563 SacII CC_GC'GG Cuts at: 2103 2103 Size: 5563 SalI G'TCGA_C Cuts at: 1936 2905 1936 Size: 969 4594 SbfI CC_TGCA'GG Cuts at: 2915 2915 Size: 5563 ScaI AGT'ACT Cuts at: 3811 5523 3811 Size: 1712 3851 SfcI C'TryA_G Cuts at: 826 1045 2053 2911 4046 4727 4918 826 Size: 219 1008 858 1135 681 191 1471 Fragments arranged by size: 1471 1135 1008 858 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2105 2105 Size: 5563 SfoI GGC'GCC Cuts at: 2013 2013 Size: 5563 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5563 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5563 SmaI CCC'GGG Cuts at: 1872 2022 2890 1872 Size: 150 868 4545 Fragments arranged by size: 4545 868 150 SmlI C'TyrA_G Cuts at: 820 1017 1943 3667 4568 4830 820 Size: 197 926 1724 901 262 1553 Fragments arranged by size: 1724 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3354 493 Size: 2861 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5563 SphI G_CATG'C Cuts at: 1132 2921 1132 Size: 1789 3774 SspI AAT'ATT Cuts at: 5 52 3487 5 Size: 47 3435 2081 Fragments arranged by size: 3435 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5563 StyI C'CwwG_G Cuts at: 513 1065 2004 2551 2842 3002 513 Size: 552 939 547 291 160 3074 Fragments arranged by size: 3074 939 552 547 291 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3590 3607 3760 3945 4390 325 Size: 1255 2010 17 153 185 445 1498 Fragments arranged by size: 2010 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3809 5521 96 Size: 274 80 33 51 1443 1832 1712 138 Fragments arranged by size: 1832 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2279 2311 2468 2500 5523 5555 2279 Size: 32 157 32 3023 32 2287 Fragments arranged by size: 3023 2287 157 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5563 XbaI T'CTAG_A Cuts at: 2899 2899 Size: 5563 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2131 2131 Size: 5563 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5563 XmaI C'CCGG_G Cuts at: 1870 2020 2888 1870 Size: 150 868 4545 Fragments arranged by size: 4545 868 150 XmnI GAAnn'nnTTC Cuts at: 2691 3692 2691 Size: 1001 4562 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3371 276 Size: 53 83 186 936 1837 2468 Fragments arranged by size: 2468 1837 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SrfI SwaI