(Linear) (Six-base) MAPSORT of: KIEE3340.seq Check: 5275 from: 1 to: 2952 KIEE3340.orf With 182 enzymes: SgfI * March 22, 2012 16:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 769 2952 Size: 769 2183 AatII G_ACGT'C Cuts at: 0 1009 2952 Size: 1009 1943 AccI GT'mk_AC Cuts at: 0 410 2952 Size: 410 2542 AclI AA'CG_TT Cuts at: 0 61 2952 Size: 61 2891 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 863 1978 2390 2952 Size: 863 1115 412 562 Fragments arranged by size: 1115 863 562 412 AgeI A'CCGG_T Cuts at: 0 913 2952 Size: 913 2039 AleI CACnn'nnGTG Cuts at: 0 2686 2952 Size: 2686 266 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 623 2952 Size: 623 2329 AlwNI CAG_nnn'CTG Cuts at: 0 46 1575 2703 2952 Size: 46 1529 1128 249 Fragments arranged by size: 1529 1128 249 46 ApaI G_GGCC'C Cuts at: 0 246 1194 2952 Size: 246 948 1758 Fragments arranged by size: 1758 948 246 ApoI r'AATT_y Cuts at: 0 603 2952 Size: 603 2349 AvaI C'yCGr_G Cuts at: 0 662 2952 Size: 662 2290 BamHI G'GATC_C Cuts at: 0 310 1803 2952 Size: 310 1493 1149 Fragments arranged by size: 1493 1149 310 BanI G'GyrC_C Cuts at: 0 741 1025 2952 Size: 741 284 1927 Fragments arranged by size: 1927 741 284 BanII G_rGCy'C Cuts at: 0 197 246 1194 2952 Size: 197 49 948 1758 Fragments arranged by size: 1758 948 197 49 BbsI GAAGACnn'nnnn_ Cuts at: 0 180 564 2852 2952 Size: 180 384 2288 100 Fragments arranged by size: 2288 384 180 100 BbvCI CC'TCA_GC Cuts at: 0 1603 2952 Size: 1603 1349 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 171 205 743 777 1952 1986 2952 Size: 171 34 538 34 1175 34 966 Fragments arranged by size: 1175 966 538 171 34 34 34 BclI T'GATC_A Cuts at: 0 2081 2952 Size: 2081 871 BfrBI ATG'CAT Cuts at: 0 674 2952 Size: 674 2278 BglI GCCn_nnn'nGGC Cuts at: 0 750 2952 Size: 750 2202 BglII A'GATC_T Cuts at: 0 1775 2952 Size: 1775 1177 BlpI GC'TnA_GC Cuts at: 0 2758 2952 Size: 2758 194 Bme1580I G_kGCm'C Cuts at: 0 246 670 704 1142 1194 1524 2952 Size: 246 424 34 438 52 330 1428 Fragments arranged by size: 1428 438 424 330 246 52 34 BmgBI CAC'GTC Cuts at: 0 699 1266 2952 Size: 699 567 1686 Fragments arranged by size: 1686 699 567 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 369 453 665 1455 2952 Size: 369 84 212 790 1497 Fragments arranged by size: 1497 790 369 212 84 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 926 1039 1221 1720 2068 2290 2360 2803 Size: 926 113 182 499 348 222 70 443 Cuts at: 2803 2952 Size: 149 Fragments arranged by size: 926 499 443 348 222 182 149 113 70 Bpu10I CC'TnA_GC Cuts at: 0 1603 2173 2952 Size: 1603 570 779 Fragments arranged by size: 1603 779 570 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2647 2952 Size: 2647 305 BsaI GGTCTCn'nnnn_ Cuts at: 0 316 2460 2952 Size: 316 2144 492 Fragments arranged by size: 2144 492 316 BsaAI yAC'GTr Cuts at: 0 1941 2952 Size: 1941 1011 BsaBI GATnn'nnATC Cuts at: 0 1774 2952 Size: 1774 1178 BsaHI Gr'CG_yC Cuts at: 0 889 1006 2952 Size: 889 117 1946 Fragments arranged by size: 1946 889 117 BsaWI w'CCGG_w Cuts at: 0 913 1406 2591 2645 2952 Size: 913 493 1185 54 307 Fragments arranged by size: 1185 913 493 307 54 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 8 11 38 41 847 859 877 889 Size: 8 3 27 3 806 12 18 12 Cuts at: 889 2323 2353 2952 Size: 1434 30 599 Fragments arranged by size: 1434 806 599 30 27 18 12 12 8 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 16 1356 1431 2952 Size: 16 1340 75 1521 Fragments arranged by size: 1521 1340 75 16 BseYI C'CCAG_C Cuts at: 0 93 589 1149 2696 2952 Size: 93 496 560 1547 256 Fragments arranged by size: 1547 560 496 256 93 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 885 2952 Size: 885 2067 BsiEI CG_ry'CG Cuts at: 0 1643 2712 2952 Size: 1643 1069 240 Fragments arranged by size: 1643 1069 240 BsiHKAI G_wGCw'C Cuts at: 0 646 2952 Size: 646 2306 BsmI GAATG_Cn' Cuts at: 0 2903 2952 Size: 2903 49 BsmBI CGTCTCn'nnnn_ Cuts at: 0 896 997 1176 1272 2952 Size: 896 101 179 96 1680 Fragments arranged by size: 1680 896 179 101 96 Bsp1286I G_dGCh'C Cuts at: 0 197 246 646 670 704 1142 1194 1524 Size: 197 49 400 24 34 438 52 330 Cuts at: 1524 2952 Size: 1428 Fragments arranged by size: 1428 438 400 330 197 52 49 34 24 BspEI T'CCGG_A Cuts at: 0 2645 2952 Size: 2645 307 BspHI T'CATG_A Cuts at: 0 2078 2952 Size: 2078 874 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 769 2952 Size: 769 2183 BsrBI CCG'CTC Cuts at: 0 875 928 2714 2952 Size: 875 53 1786 238 Fragments arranged by size: 1786 875 238 53 BsrDI GCAATG_nn' Cuts at: 0 189 359 416 987 2951 2952 Size: 189 170 57 571 1964 1 Fragments arranged by size: 1964 571 189 170 57 1 BsrFI r'CCGG_y Cuts at: 0 233 913 2522 2600 2952 Size: 233 680 1609 78 352 Fragments arranged by size: 1609 680 352 233 78 BsrGI T'GTAC_A Cuts at: 0 1721 1898 2952 Size: 1721 177 1054 Fragments arranged by size: 1721 1054 177 BstAPI GCAn_nnn'nTGC Cuts at: 0 46 806 1085 1562 2952 Size: 46 760 279 477 1390 Fragments arranged by size: 1390 760 477 279 46 BstEII G'GTnAC_C Cuts at: 0 2058 2826 2952 Size: 2058 768 126 Fragments arranged by size: 2058 768 126 BstXI CCAn_nnnn'nTGG Cuts at: 0 1017 1149 2343 2772 2952 Size: 1017 132 1194 429 180 Fragments arranged by size: 1194 1017 429 180 132 BstYI r'GATC_y Cuts at: 0 310 1403 1775 1803 2322 2354 2891 2952 Size: 310 1093 372 28 519 32 537 61 Fragments arranged by size: 1093 537 519 372 310 61 32 28 Bsu36I CC'TnA_GG Cuts at: 0 2125 2952 Size: 2125 827 BtgI C'CryG_G Cuts at: 0 1445 2537 2744 2952 Size: 1445 1092 207 208 Fragments arranged by size: 1445 1092 208 207 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 64 232 768 2166 2370 2952 Size: 64 168 536 1398 204 582 Fragments arranged by size: 1398 582 536 204 168 64 BtsI GCAGTG_nn' Cuts at: 0 47 617 855 2798 2952 Size: 47 570 238 1943 154 Fragments arranged by size: 1943 570 238 154 47 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1533 1568 2952 Size: 1533 35 1384 Fragments arranged by size: 1533 1384 35 EaeI y'GGCC_r Cuts at: 0 104 1360 1448 1640 1670 1943 2952 Size: 104 1256 88 192 30 273 1009 Fragments arranged by size: 1256 1009 273 192 104 88 30 EagI C'GGCC_G Cuts at: 0 1640 2952 Size: 1640 1312 EarI CTCTTCn'nnn_ Cuts at: 0 560 1859 2952 Size: 560 1299 1093 Fragments arranged by size: 1299 1093 560 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 863 926 1039 1221 1720 1978 2068 2290 Size: 863 63 113 182 499 258 90 222 Cuts at: 2290 2360 2390 2803 2952 Size: 70 30 413 149 Fragments arranged by size: 863 499 413 258 222 182 149 113 90 70 63 30 EcoO109I rG'GnC_Cy Cuts at: 0 242 393 973 2229 2952 Size: 242 151 580 1256 723 Fragments arranged by size: 1256 723 580 242 151 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 342 396 1266 1581 2952 Size: 342 54 870 315 1371 Fragments arranged by size: 1371 870 342 315 54 HaeII r_GCGC'y Cuts at: 0 2765 2952 Size: 2765 187 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 93 125 631 663 1579 1611 2302 2334 Size: 93 32 506 32 916 32 691 32 Cuts at: 2334 2339 2371 2952 Size: 5 32 581 Fragments arranged by size: 916 691 581 506 93 32 32 32 32 32 5 HincII GTy'rAC Cuts at: 0 1275 2256 2625 2952 Size: 1275 981 369 327 Fragments arranged by size: 1275 981 369 327 MfeI C'AATT_G Cuts at: 0 207 2952 Size: 207 2745 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 889 890 2306 2510 2952 Size: 889 1 1416 204 442 Fragments arranged by size: 1416 889 442 204 1 MscI TGG'CCA Cuts at: 0 1362 1450 1672 1945 2952 Size: 1362 88 222 273 1007 Fragments arranged by size: 1362 1007 273 222 88 MslI CAynn'nnrTG Cuts at: 0 262 2686 2770 2833 2952 Size: 262 2424 84 63 119 Fragments arranged by size: 2424 262 119 84 63 MspA1I CmG'CkG Cuts at: 0 1115 1149 1614 1639 2551 2823 2952 Size: 1115 34 465 25 912 272 129 Fragments arranged by size: 1115 912 465 272 129 34 25 NaeI GCC'GGC Cuts at: 0 2602 2952 Size: 2602 350 NcoI C'CATG_G Cuts at: 0 1445 2537 2952 Size: 1445 1092 415 Fragments arranged by size: 1445 1092 415 NgoMIV G'CCGG_C Cuts at: 0 2600 2952 Size: 2600 352 NsiI A_TGCA'T Cuts at: 0 676 2952 Size: 676 2276 NspI r_CATG'y Cuts at: 0 674 800 956 2952 Size: 674 126 156 1996 Fragments arranged by size: 1996 674 156 126 PasI CC'CwG_GG Cuts at: 0 1765 2952 Size: 1765 1187 PflMI CCAn_nnn'nTGG Cuts at: 0 2163 2952 Size: 2163 789 PfoI T'CCnGG_A Cuts at: 0 1391 1820 2306 2952 Size: 1391 429 486 646 Fragments arranged by size: 1391 646 486 429 PpuMI rG'GwC_Cy Cuts at: 0 973 2229 2952 Size: 973 1256 723 Fragments arranged by size: 1256 973 723 PspOMI G'GGCC_C Cuts at: 0 242 1190 2952 Size: 242 948 1762 Fragments arranged by size: 1762 948 242 PstI C_TGCA'G Cuts at: 0 90 289 426 816 885 2952 Size: 90 199 137 390 69 2067 Fragments arranged by size: 2067 390 199 137 90 69 PvuII CAG'CTG Cuts at: 0 1149 1614 2551 2823 2952 Size: 1149 465 937 272 129 Fragments arranged by size: 1149 937 465 272 129 RsrII CG'GwC_CG Cuts at: 0 911 2952 Size: 911 2041 SbfI CC_TGCA'GG Cuts at: 0 816 2952 Size: 816 2136 SexAI A'CCwGG_T Cuts at: 0 173 2264 2952 Size: 173 2091 688 Fragments arranged by size: 2091 688 173 SfcI C'TryA_G Cuts at: 0 86 285 422 812 881 1686 2952 Size: 86 199 137 390 69 805 1266 Fragments arranged by size: 1266 805 390 199 137 86 69 SmlI C'TyrA_G Cuts at: 0 2662 2952 Size: 2662 290 SphI G_CATG'C Cuts at: 0 800 956 2952 Size: 800 156 1996 Fragments arranged by size: 1996 800 156 StyI C'CwwG_G Cuts at: 0 1033 1445 1664 1832 2342 2537 2870 2952 Size: 1033 412 219 168 510 195 333 82 Fragments arranged by size: 1033 510 412 333 219 195 168 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2550 2845 2952 Size: 2550 295 107 Fragments arranged by size: 2550 295 107 TatI w'GTAC_w Cuts at: 0 1721 1898 2780 2952 Size: 1721 177 882 172 Fragments arranged by size: 1721 882 177 172 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 847 879 2307 2339 2952 Size: 847 32 1428 32 613 Fragments arranged by size: 1428 847 613 32 32 Tth111I GACn'n_nGTC Cuts at: 0 355 2952 Size: 355 2597 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 448 1225 2952 Size: 448 777 1727 Fragments arranged by size: 1727 777 448 ZraI GAC'GTC Cuts at: 0 1007 2952 Size: 1007 1945 Enzymes that do cut: AarI AatII AccI AclI AcuI AgeI AleI AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EagI EarI Eco57MI EcoO109I FalI HaeII Hin4I HincII MfeI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII RsrII SbfI SexAI SfcI SmlI SphI StyI TaqII TatI TstI Tth111I XcmI ZraI Enzymes that do not cut: Acc65I AfeI AflII AflIII AhdI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BciVI BmrI BmtI BsiWI BssHII BssSI BstBI BstZ17I ClaI DraI DraIII DrdI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MluI NarI NdeI NheI NotI NruI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI SacI SacII SalI SanDI SapI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI XmaI XmnI