Restriction map for
pFN21AE6278
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbsI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI BspDI BspHI BspLU11I BspMI
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MluI MluNI
MscI MseI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
TaqI Tru9I Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccIII 32 TCCGGA T^CCGGA
AflIII 50 ACGTGT A^C[GA][CT]GT
AlwI 587 GGATC GGATC(4/5)
Asp700I 372 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 46 GAGCAC G[AT]GC[AT]^C
BbrPI 49 CACGTG CAC^GTG
BbvCI 325 GCTGAGG CCTCAGC(-5/-2)
BcnI 225 CCGGG CC^[GC]GG
BpmI 461 CTGGAG CTGGAG(16/14)
BsaAI 49 CACGTG [CT]AC^GT[GA]
BsaWI 32 TCCGGA [AT]^CCGG[AT]
BseAI 32 TCCGGA T^CCGGA
BsiHKAI 46 GAGCAC G[AT]GC[AT]^C
Bsp1407I 557 TGTACA T^GTACA
BspEI 32 TCCGGA T^CCGGA
BsrDI 605 GCAATG GCAATG(2/0)
BsrFI 359 ACCGGC [GA]^CCGG[CT]
BsrGI 557 TGTACA T^GTACA
BstXI 77 CCAGCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 359 ACCGGC [GA]^CCGG[CT]
EcoT14I 108 CCAAGG C^C[AT][AT]GG
MboII 380 TCTTC GAAGA(8/7)
MroI 32 TCCGGA T^CCGGA
MspA1I 78 CAGCTG C[AC]G^C[GT]G
NciI 225 CCGGG CC^[GC]GG
PflMI 212 CCAAGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 49 CACGTG CAC^GTG
PmlI 49 CACGTG CAC^GTG
PvuII 78 CAGCTG CAG^CTG
SspBI 557 TGTACA T^GTACA
StuI 527 AGGCCT AGG^CCT
StyI 108 CCAAGG C^C[AT][AT]GG
Tsp509I 430 AATT ^AATT
Van91I 212 CCAAGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 372 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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ApaI 62 GGGCCC GGGCC^C
173 GGGCCC
AvaII 183 GGTCC G^G[AT]CC
622 GGTCC
BanI 112 GGCACC G^G[CT][GA]CC
139 GGCACC
BsrI 218 ACTGG ACTGG(1/-1)
388 CCAGT
BstF5I 105 CATCC GGATG(2/0)
35 GGATG
DpnII 417 GATC ^GATC
588 GATC
FauI 5 CCCGC CCCGC(4/6)
563 CCCGC
FokI 105 CATCC GGATG(9/13)
35 GGATG
MaeII 50 ACGT A^CGT
245 ACGT
MboI 417 GATC ^GATC
588 GATC
MslI 49 CACGTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
198 CATCATTATG
MwoI 104 GCATCCAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
660 GCTGCTGCAGC
NdeII 417 GATC ^GATC
588 GATC
PpuMI 182 GGGTCCC [GA]G^G[AT]CC[CT]
621 AGGTCCC
Sau3AI 417 GATC ^GATC
588 GATC
ScaI 122 AGTACT AGT^ACT
215 AGTACT
SfaNI 104 GCATC GCATC(5/9)
566 GCATC
TspRI 100 CACTG CA[GC]TG[ACGT][ACGT]^
128 CAGTG
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AfaI AluI BanII BbvI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI Cac8I Cfr13I
DdeI DraII EcoO109I EcoRII Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MnlI MspI MvaI
NlaIII NlaIV PstI RsaI Sau96I
ScrFI SfcI TfiI TseI Tsp45I