(Linear) (Six-base) MAPSORT of: KIEE6278.seq Check: 8577 from: 1 to: 684 KIEE6278.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 150 264 684 Size: 150 114 420 Fragments arranged by size: 420 150 114 AflIII A'CryG_T Cuts at: 0 50 684 Size: 50 634 AleI CACnn'nnGTG Cuts at: 0 53 684 Size: 53 631 ApaI G_GGCC'C Cuts at: 0 66 177 684 Size: 66 111 507 Fragments arranged by size: 507 111 66 BanI G'GyrC_C Cuts at: 0 112 139 684 Size: 112 27 545 Fragments arranged by size: 545 112 27 BanII G_rGCy'C Cuts at: 0 66 177 682 684 Size: 66 111 505 2 Fragments arranged by size: 505 111 66 2 BbvCI CC'TCA_GC Cuts at: 0 326 684 Size: 326 358 Bme1580I G_kGCm'C Cuts at: 0 66 177 684 Size: 66 111 507 Fragments arranged by size: 507 111 66 BmrI ACTGGGnnnn_n' Cuts at: 0 228 382 684 Size: 228 154 302 Fragments arranged by size: 302 228 154 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 82 684 Size: 82 602 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 482 684 Size: 482 202 Bpu10I CC'TnA_GC Cuts at: 0 326 684 Size: 326 358 BsaAI yAC'GTr Cuts at: 0 51 684 Size: 51 633 BsaWI w'CCGG_w Cuts at: 0 32 684 Size: 32 652 BseYI C'CCAG_C Cuts at: 0 76 80 505 684 Size: 76 4 425 179 Fragments arranged by size: 425 179 76 4 BsiHKAI G_wGCw'C Cuts at: 0 50 684 Size: 50 634 Bsp1286I G_dGCh'C Cuts at: 0 50 66 177 682 684 Size: 50 16 111 505 2 Fragments arranged by size: 505 111 50 16 2 BspEI T'CCGG_A Cuts at: 0 32 684 Size: 32 652 BsrDI GCAATG_nn' Cuts at: 0 612 684 Size: 612 72 BsrFI r'CCGG_y Cuts at: 0 359 684 Size: 359 325 BsrGI T'GTAC_A Cuts at: 0 557 684 Size: 557 127 BstXI CCAn_nnnn'nTGG Cuts at: 0 84 684 Size: 84 600 BtsI GCAGTG_nn' Cuts at: 0 99 684 Size: 99 585 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 150 264 482 684 Size: 150 114 218 202 Fragments arranged by size: 218 202 150 114 EcoO109I rG'GnC_Cy Cuts at: 0 62 63 173 174 183 622 684 Size: 62 1 110 1 9 439 62 Fragments arranged by size: 439 110 62 62 9 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 393 396 684 Size: 393 3 288 Fragments arranged by size: 393 288 3 MslI CAynn'nnrTG Cuts at: 0 53 202 684 Size: 53 149 482 Fragments arranged by size: 482 149 53 MspA1I CmG'CkG Cuts at: 0 80 684 Size: 80 604 PasI CC'CwG_GG Cuts at: 0 17 684 Size: 17 667 PflMI CCAn_nnn'nTGG Cuts at: 0 218 684 Size: 218 466 PmlI CAC'GTG Cuts at: 0 51 684 Size: 51 633 PpuMI rG'GwC_Cy Cuts at: 0 183 622 684 Size: 183 439 62 Fragments arranged by size: 439 183 62 PspOMI G'GGCC_C Cuts at: 0 62 173 684 Size: 62 111 511 Fragments arranged by size: 511 111 62 PstI C_TGCA'G Cuts at: 0 155 320 668 684 Size: 155 165 348 16 Fragments arranged by size: 348 165 155 16 PvuII CAG'CTG Cuts at: 0 80 684 Size: 80 604 SanDI GG'GwC_CC Cuts at: 0 183 684 Size: 183 501 ScaI AGT'ACT Cuts at: 0 124 217 684 Size: 124 93 467 Fragments arranged by size: 467 124 93 SfcI C'TryA_G Cuts at: 0 151 316 664 684 Size: 151 165 348 20 Fragments arranged by size: 348 165 151 20 StuI AGG'CCT Cuts at: 0 529 684 Size: 529 155 StyI C'CwwG_G Cuts at: 0 108 684 Size: 108 576 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 72 229 684 Size: 72 157 455 Fragments arranged by size: 455 157 72 TatI w'GTAC_w Cuts at: 0 122 215 557 684 Size: 122 93 342 127 Fragments arranged by size: 342 127 122 93 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 430 462 684 Size: 430 32 222 Fragments arranged by size: 430 222 32 XmnI GAAnn'nnTTC Cuts at: 0 376 684 Size: 376 308 Enzymes that do cut: AcuI AflIII AleI ApaI BanI BanII BbvCI Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaWI BseYI BsiHKAI Bsp1286I BspEI BsrDI BsrFI BsrGI BstXI BtsI Eco57MI EcoO109I FalI MslI MspA1I PasI PflMI PmlI PpuMI PspOMI PstI PvuII SanDI ScaI SfcI StuI StyI TaqII TatI TstI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BpuEI BsaI BsaBI BsaHI BsaXI BseRI BsgI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI