################################## # NO CUT ENZYMES ( 186 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1286I Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AciI 97 GCGG CCGC(-3/-1) AfaI 78 GTAC GT^AC AseI 385 ATTAAT AT^TAAT AsnI 385 ATTAAT AT^TAAT AvaI 56 CCCGAG C^[CT]CG[GA]G BseRI 16 GAGGAG GAGGAG(10/8) BsgI 244 GTGCAG GTGCAG(16/14) BsmAI 744 GAGAC GTCTC(1/5) BsoBI 56 CCCGAG C^[CT]CG[GA]G BspDI 251 ATCGAT AT^CGAT BsrI 451 CCAGT ACTGG(1/-1) ClaI 251 ATCGAT AT^CGAT DraI 169 TTTAAA TTT^AAA EcoRI 215 GAATTC G^AATTC FauI 97 GCGGG CCCGC(4/6) MaeII 242 ACGT A^CGT NspI 438 GCATGT [GA]CATG^[CT] PshBI 385 ATTAAT AT^TAAT PstI 457 CTGCAG CTGCA^G RsaI 78 GTAC GT^AC SapI 269 GCTCTTC GCTCTTC(1/4) SspI 410 AATATT AAT^ATT TaqI 252 TCGA T^CGA TthHB8I 252 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### Asp700I 67 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 409 GAATATTTTC BbvI 459 GCAGC GCAGC(8/12) 456 GCTGC BstF5I 503 CATCC GGATG(2/0) 629 CATCC DdeI 11 CTCAG C^T[ACGT]AG 824 CTAAG EarI 270 CTCTTC CTCTTC(1/4) 67 GAAGAG Fnu4HI 456 GCTGC GC^[ACGT]GC 459 GCAGC FokI 503 CATCC GGATG(9/13) 629 CATCC HphI 570 TCACC GGTGA(8/7) 696 TCACC ItaI 456 GCTGC GC^[ACGT]GC 459 GCAGC Ksp632I 270 CTCTTC CTCTTC(1/4) 67 GAAGAG MslI 557 CATATAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 683 CATATAAATG SfcI 327 CTACAG C^T[GA][CT]AG 457 CTGCAG TseI 456 GCTGC G^C[AT]GC 459 GCAGC XmnI 67 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 409 GAATATTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AcsI AluI ApoI AvaII BbsI BglII BpuAI BsiYI BslI BstYI Cfr13I DpnII HaeIII HinfI MaeIII MboI MboII MflI MnlI MseI NdeII NlaIII NlaIV PleI Sau3AI Sau96I SfaNI SmlI TfiI Tru9I Tsp45I Tsp509I TspRI XhoII