(Circular) (Six-base) MAPSORT of: pFN21AB6316.seq Check: 6360 from: 1 to: 5548 pFN21AB6316 5548 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3358 278 Size: 53 83 186 936 1822 2468 Fragments arranged by size: 2468 1822 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2891 1937 Size: 954 4594 Acc65I G'GTAC_C Cuts at: 2869 2869 Size: 5548 AclI AA'CG_TT Cuts at: 1563 3675 4048 1563 Size: 2112 373 3063 Fragments arranged by size: 3063 2112 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2081 3611 4995 5263 1632 Size: 449 1530 1384 268 1917 Fragments arranged by size: 1917 1530 1384 449 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5351 AflIII A'CryG_T Cuts at: 1203 4447 5424 1203 Size: 3244 977 1327 Fragments arranged by size: 3244 1327 977 AgeI A'CCGG_T Cuts at: 4379 4379 Size: 5548 AhdI GACnn_n'nnGTC Cuts at: 4277 4277 Size: 5548 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5548 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5548 AlwNI CAG_nnn'CTG Cuts at: 3291 4863 3291 Size: 1572 3976 ApaLI G'TGCA_C Cuts at: 3605 4761 3605 Size: 1156 4392 ApoI r'AATT_y Cuts at: 1493 2050 2220 2320 2857 3083 3235 5344 1493 Size: 557 170 100 537 226 152 2109 1697 Fragments arranged by size: 2109 1697 557 537 226 170 152 100 AseI AT'TA_AT Cuts at: 160 1032 2391 4102 160 Size: 872 1359 1711 1606 Fragments arranged by size: 1711 1606 1359 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 AvaI C'yCGr_G Cuts at: 1870 1943 2061 2873 1870 Size: 73 118 812 4545 Fragments arranged by size: 4545 812 118 73 BamHI G'GATC_C Cuts at: 2878 3245 2878 Size: 367 5181 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2869 4324 618 Size: 325 217 627 1082 1455 1842 Fragments arranged by size: 1842 1455 1082 627 325 217 BanII G_rGCy'C Cuts at: 1391 2867 1391 Size: 1476 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2242 2508 2634 928 Size: 971 343 266 126 3842 Fragments arranged by size: 3842 971 343 266 126 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3739 3773 1903 Size: 34 1802 34 3678 Fragments arranged by size: 3678 1802 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3440 4650 3440 Size: 1210 4338 BclI T'GATC_A Cuts at: 1548 1548 Size: 5548 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4159 136 Size: 107 122 71 631 605 2487 1525 Fragments arranged by size: 2487 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2360 2539 2665 5543 2360 Size: 179 126 2878 2365 Fragments arranged by size: 2878 2365 179 126 BlpI GC'TnA_GC Cuts at: 2965 2965 Size: 5548 Bme1580I G_kGCm'C Cuts at: 1790 3609 4765 1790 Size: 1819 1156 2573 Fragments arranged by size: 2573 1819 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5548 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3279 4237 442 Size: 416 53 83 399 24 1862 958 1753 Fragments arranged by size: 1862 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5548 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4208 1239 Size: 2969 2579 Bpu10I CC'TnA_GC Cuts at: 2016 2016 Size: 5548 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2394 2564 2690 3673 4538 4836 2394 Size: 170 126 983 865 298 3106 Fragments arranged by size: 3106 983 865 298 170 126 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4211 882 Size: 617 2712 2219 Fragments arranged by size: 2712 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3339 493 Size: 711 2135 2702 Fragments arranged by size: 2702 2135 711 BsaBI GATnn'nnATC Cuts at: 5542 5542 Size: 5548 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3355 3737 4372 275 Size: 53 83 186 936 1822 382 635 1451 Fragments arranged by size: 1822 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3981 4379 4653 4800 5211 3981 Size: 398 274 147 411 4318 Fragments arranged by size: 4318 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5323 Fragments arranged by size: 5323 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 1186 Size: 850 4698 BseYI C'CCAG_C Cuts at: 2555 2681 4751 5247 2555 Size: 126 2070 496 2856 Fragments arranged by size: 2856 2070 496 126 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2270 1163 Size: 573 534 4441 Fragments arranged by size: 4441 573 534 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2998 3759 4787 1540 Size: 275 186 997 761 1028 2301 Fragments arranged by size: 2301 1028 997 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3609 3694 4765 3609 Size: 85 1071 4392 Fragments arranged by size: 4392 1071 85 BsmI GAATG_Cn' Cuts at: 3059 3152 3059 Size: 93 5455 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2867 3609 3694 4765 1391 Size: 399 1077 742 85 1071 2174 Fragments arranged by size: 2174 1077 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5211 5211 Size: 5548 BspHI T'CATG_A Cuts at: 3437 5167 3437 Size: 1730 3818 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2903 844 Size: 2059 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3435 1394 Size: 429 1612 3507 Fragments arranged by size: 3507 1612 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4043 4217 65 Size: 1183 2795 174 1396 Fragments arranged by size: 2795 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4192 4379 1700 Size: 110 2382 187 2869 Fragments arranged by size: 2869 2382 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5548 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5548 BssSI C'ACGA_G Cuts at: 3608 4620 3608 Size: 1012 4536 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5548 BstBI TT'CG_AA Cuts at: 4352 4352 Size: 5548 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5548 BstYI r'GATC_y Cuts at: 1972 2360 2539 2665 2878 3245 3630 3647 5099 Size: 388 179 126 213 367 385 17 1452 Cuts at: 5099 5185 5197 5543 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 388 385 367 346 213 179 126 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4996 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4809 Fragments arranged by size: 4809 683 56 BtsI GCAGTG_nn' Cuts at: 2444 3064 3858 3878 2444 Size: 620 794 20 4114 Fragments arranged by size: 4114 794 620 20 ClaI AT'CG_AT Cuts at: 1554 2257 1554 Size: 703 4845 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5513 DraI TTT'AAA Cuts at: 2176 2852 2176 Size: 676 4872 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4555 809 Size: 507 3239 1802 Fragments arranged by size: 3239 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2995 3884 5513 Size: 54 1425 50 275 18 1165 889 1629 Cuts at: 5513 8 Size: 43 Fragments arranged by size: 1629 1425 1165 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2995 1537 Size: 275 1183 4090 Fragments arranged by size: 4090 1183 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2067 2281 3478 1353 Size: 714 214 1197 3423 Fragments arranged by size: 3423 1197 714 214 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4135 4509 4655 387 Size: 1064 138 2546 374 146 1280 Fragments arranged by size: 2546 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 3611 4208 4995 5263 1239 Size: 393 449 1530 597 787 268 1524 Fragments arranged by size: 1530 1524 787 597 449 393 268 EcoRI G'AATT_C Cuts at: 2220 2857 3235 2220 Size: 637 378 4533 Fragments arranged by size: 4533 637 378 FspI TGC'GCA Cuts at: 4054 4054 Size: 5548 HaeII r_GCGC'y Cuts at: 1678 4695 1678 Size: 3017 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2599 2631 4189 4221 4263 Size: 32 135 32 1027 32 1558 32 42 Cuts at: 4263 4295 1373 Size: 32 2626 Fragments arranged by size: 2626 1558 1027 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2892 3138 669 Size: 988 281 954 246 3079 Fragments arranged by size: 3079 988 954 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5548 HpaI GTT'AAC Cuts at: 3138 3138 Size: 5548 KpnI G_GTAC'C Cuts at: 2873 2873 Size: 5548 MfeI C'AATT_G Cuts at: 3147 3147 Size: 5548 MluI A'CGCG_T Cuts at: 5424 5424 Size: 5548 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4662 4846 1305 Size: 3357 184 2007 Fragments arranged by size: 3357 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3726 Fragments arranged by size: 3726 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2566 2692 3506 3865 4024 518 Size: 648 1400 126 814 359 159 2042 Fragments arranged by size: 2042 1400 814 648 359 159 126 MspA1I CmG'CkG Cuts at: 1606 2964 3641 4789 5034 1606 Size: 1358 677 1148 245 2120 Fragments arranged by size: 2120 1358 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4996 NdeI CA'TA_TG Cuts at: 387 387 Size: 5548 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5548 NotI GC'GGCC_GC Cuts at: 2995 2995 Size: 5548 NspI r_CATG'y Cuts at: 1132 1229 2447 2906 4451 1132 Size: 97 1218 459 1545 2229 Fragments arranged by size: 2229 1545 1218 459 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5548 PciI A'CATG_T Cuts at: 4447 4447 Size: 5548 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5548 PmeI GTTT'AAAC Cuts at: 2852 2852 Size: 5548 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3617 3649 5130 5162 3617 Size: 32 1481 32 4003 Fragments arranged by size: 4003 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5548 PsiI TTA'TAA Cuts at: 3118 3118 Size: 5548 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2075 2107 2075 Size: 32 5516 PstI C_TGCA'G Cuts at: 830 2466 2900 830 Size: 1636 434 3478 Fragments arranged by size: 3478 1636 434 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5548 SalI G'TCGA_C Cuts at: 1936 2890 1936 Size: 954 4594 SapI GCTCTTCn'nnn_ Cuts at: 2281 2281 Size: 5548 SbfI CC_TGCA'GG Cuts at: 2900 2900 Size: 5548 ScaI AGT'ACT Cuts at: 3796 5508 3796 Size: 1712 3836 SfcI C'TryA_G Cuts at: 826 1045 2332 2462 2896 4031 4712 4903 826 Size: 219 1287 130 434 1135 681 191 1471 Fragments arranged by size: 1471 1287 1135 681 434 219 191 130 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5548 SmaI CCC'GGG Cuts at: 1872 2875 1872 Size: 1003 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2409 2543 2669 3652 4553 4815 Size: 197 926 466 134 126 983 901 262 Cuts at: 4815 820 Size: 1553 Fragments arranged by size: 1553 983 926 901 466 262 197 134 126 SnaBI TAC'GTA Cuts at: 493 3339 493 Size: 2846 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5548 SphI G_CATG'C Cuts at: 1132 2906 1132 Size: 1774 3774 SspI AAT'ATT Cuts at: 5 52 2417 3472 5 Size: 47 2365 1055 2081 Fragments arranged by size: 2365 2081 1055 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5548 StyI C'CwwG_G Cuts at: 513 1065 2987 513 Size: 552 1922 3074 Fragments arranged by size: 3074 1922 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2196 3575 3592 3745 3930 4375 325 Size: 1255 616 1379 17 153 185 445 1498 Fragments arranged by size: 1498 1379 1255 616 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3794 5506 96 Size: 274 80 33 51 1443 1817 1712 138 Fragments arranged by size: 1817 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5508 5540 5508 Size: 32 5516 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5548 XbaI T'CTAG_A Cuts at: 2884 2884 Size: 5548 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5548 XmaI C'CCGG_G Cuts at: 1870 2873 1870 Size: 1003 4545 XmnI GAAnn'nnTTC Cuts at: 2076 2418 3677 2076 Size: 342 1259 3947 Fragments arranged by size: 3947 1259 342 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3356 276 Size: 53 83 186 936 1822 2468 Fragments arranged by size: 2468 1822 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI