Restriction map for
pFN21AE5013
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AluI
Alw44I AlwI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstXI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DpnII
DraI DraIII Eam1105I Eco47III EcoO65I
EcoRV EcoT22I FbaI FokI FseI
FspI HgaI HindIII HpaI HphI
KpnI KspI MaeII MaeIII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MunI NaeI
NcoI NdeI NdeII NgoMIV NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI Sau3AI SbfI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 193 GGCGCC G[GA]^CG[CT]C
AfaI 341 GTAC GT^AC
AocI 84 CCTGAGG CC^T[ACGT]AGG
Asp700I 654 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 277 GGACC G^G[AT]CC
BanI 193 GGCGCC G^G[CT][GA]CC
BbeI 193 GGCGCC GGCGC^C
BbvCI 720 CCTCAGC CCTCAGC(-5/-2)
BfaI 368 CTAG C^TAG
BpmI 457 CTCCAG CTGGAG(16/14)
BsaHI 193 GGCGCC G[GA]^CG[CT]C
BseRI 328 GAGGAG GAGGAG(10/8)
BsgI 526 GTGCAG GTGCAG(16/14)
BsmAI 14 GTCTC GTCTC(1/5)
BsrI 102 CCAGT ACTGG(1/-1)
BssHII 140 GCGCGC G^CGCGC
BstAPI 382 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 84 CCTGAGG CC^T[ACGT]AGG
DrdI 727 GACCAGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 580 CCGTGG C^C[GA][CT]GG
EarI 283 GAAGAG CTCTTC(1/4)
Eco81I 84 CCTGAGG CC^T[ACGT]AGG
EcoNI 453 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 497 CCAAGG C^C[AT][AT]GG
Hin1I 193 GGCGCC G[GA]^CG[CT]C
HincII 667 GTCAAC GT[CT]^[GA]AC
HindII 667 GTCAAC GT[CT]^[GA]AC
KasI 193 GGCGCC G^GCGCC
Ksp632I 283 GAAGAG CTCTTC(1/4)
MaeI 368 CTAG C^TAG
MslI 519 CATGACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 317 CCGCTG C[AC]G^C[GT]G
NarI 193 GGCGCC GG^CGCC
NlaIII 519 CATG CATG^
NotI 313 GCGGCCGC GC^GGCCGC
RsaI 341 GTAC GT^AC
SapI 283 GAAGAGC GCTCTTC(1/4)
SfoI 193 GGCGCC GGC^GCC
SmaI 594 CCCGGG CCC^GGG
StyI 497 CCAAGG C^C[AT][AT]GG
TfiI 48 GAATC G^A[AT]TC
XmaCI 594 CCCGGG C^CCGGG
XmaI 594 CCCGGG C^CCGGG
XmnI 654 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AcsI 658 GAATTC [GA]^AATT[CT]
673 GAATTC
AlwNI 383 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
793 CAGGCGCTG
ApaI 501 GGGCCC GGGCC^C
602 GGGCCC
ApoI 658 GAATTC [GA]^AATT[CT]
673 GAATTC
AvaI 361 CCCGAG C^[CT]CG[GA]G
594 CCCGGG
BsoBI 361 CCCGAG C^[CT]CG[GA]G
594 CCCGGG
BsrBI 505 CCGCTC CCGCTC(-3/-3)
23 GAGCGG
DraII 500 AGGGCCC [GA]G^G[ACGT]CC[CT]
601 AGGGCCC
EagI 314 CGGCCG C^GGCCG
346 CGGCCG
EclXI 314 CGGCCG C^GGCCG
346 CGGCCG
Eco52I 314 CGGCCG C^GGCCG
346 CGGCCG
EcoO109I 500 AGGGCCC [GA]G^G[ACGT]CC[CT]
601 AGGGCCC
EcoRI 658 GAATTC G^AATTC
673 GAATTC
PleI 64 GACTC GAGTC(4/5)
364 GAGTC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI BanII BbvI BcnI
BmyI BsaJI BsiEI BsiYI BslI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI EaeI EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MboII MnlI MspI MvaI MvnI
MwoI NciI NlaIV PstI Sau96I
ScrFI SfcI TseI Tsp509I TspRI