Restriction map for
pFN21AE5013
(Circular) (Six-base) MAPSORT of: pFN21AE5013.seq Check: 5932 from: 1 to: 5515
pFN21AE5013 5515 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2858 1937
Size: 921 4594
Acc65I G'GTAC_C
Cuts at: 2836 2836
Size: 5515
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3578 4962 5230 1632
Size: 1946 1384 268 1917
Fragments arranged by size:
1946 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 4414 5391 1203
Size: 3211 977 1327
Fragments arranged by size:
3211 1327 977
AgeI A'CCGG_T
Cuts at: 4346 4346
Size: 5515
AhdI GACnn_n'nnGTC
Cuts at: 4244 4244
Size: 5515
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5515
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2661 1184
Size: 1477 4038
AlwNI CAG_nnn'CTG
Cuts at: 2393 2803 3258 4830 2393
Size: 410 455 1572 3078
Fragments arranged by size:
3078 1572 455 410
ApaI G_GGCC'C
Cuts at: 2510 2611 2510
Size: 101 5414
ApaLI G'TGCA_C
Cuts at: 3572 4728 3572
Size: 1156 4359
ApoI r'AATT_y
Cuts at: 1493 2663 2678 2824 3050 3202 5311 1493
Size: 1170 15 146 226 152 2109 1697
Fragments arranged by size:
2109 1697 1170 226 152 146 15
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2366 2599 2840 1870
Size: 73 423 233 241 4545
Fragments arranged by size:
4545 423 241 233 73
BamHI G'GATC_C
Cuts at: 2845 3212 2845
Size: 367 5148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2198 2836 4291 618
Size: 325 217 627 411 638 1455 1842
Fragments arranged by size:
1842 1455 638 627 411 325 217
BanII G_rGCy'C
Cuts at: 1391 2045 2450 2473 2510 2611 2834 1391
Size: 654 405 23 37 101 223 4072
Fragments arranged by size:
4072 654 405 223 101 37 23
BbeI G_GCGC'C
Cuts at: 2202 2202
Size: 5515
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4544
BbvCI CC'TCA_GC
Cuts at: 2726 2726
Size: 5515
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3706 3740 1903
Size: 34 1769 34 3678
Fragments arranged by size:
3678 1769 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3407 4617 3407
Size: 1210 4305
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5515
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4126 136
Size: 107 122 71 631 605 2454 1525
Fragments arranged by size:
2454 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5510 5510
Size: 5515
BlpI GC'TnA_GC
Cuts at: 2932 2932
Size: 5515
Bme1580I G_kGCm'C
Cuts at: 1790 2510 2611 3576 4732 1790
Size: 720 101 965 1156 2573
Fragments arranged by size:
2573 1156 965 720 101
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5515
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3246 4204 442
Size: 416 53 83 399 24 1829 958 1753
Fragments arranged by size:
1829 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2447 4175 1239
Size: 1208 1728 2579
Fragments arranged by size:
2579 1728 1208
Bpu10I CC'TnA_GC
Cuts at: 2726 2726
Size: 5515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3640 4505 4803 3640
Size: 865 298 4352
Fragments arranged by size:
4352 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4178 882
Size: 617 2679 2219
Fragments arranged by size:
2679 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3306 493
Size: 711 2102 2702
Fragments arranged by size:
2702 2102 711
BsaBI GATnn'nnATC
Cuts at: 5509 5509
Size: 5515
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2199 3322 3704 4339
Size: 53 83 186 936 666 1123 382 635
Cuts at: 4339 275
Size: 1451
Fragments arranged by size:
1451 1123 936 666 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3948 4346 4620 4767 5178 3948
Size: 398 274 147 411 4285
Fragments arranged by size:
4285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5290
Fragments arranged by size:
5290 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 1186
Size: 1162 4353
BseYI C'CCAG_C
Cuts at: 2324 4718 5214 2324
Size: 2394 496 2625
Fragments arranged by size:
2625 2394 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2552 1163
Size: 573 816 4126
Fragments arranged by size:
4126 816 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2322 2344 2354 2965 3726 4754
Size: 275 186 321 22 10 611 761 1028
Cuts at: 4754 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 611 321 275 186 22
10
BsiHKAI G_wGCw'C
Cuts at: 3576 3661 4732 3576
Size: 85 1071 4359
Fragments arranged by size:
4359 1071 85
BsmI GAATG_Cn'
Cuts at: 3026 3119 3026
Size: 93 5422
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2450 2473 2510 2611 2834 3576
Size: 399 255 405 23 37 101 223 742
Cuts at: 3576 3661 4732 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 405 399 255 223 101
85 37 23
BspEI T'CCGG_A
Cuts at: 5178 5178
Size: 5515
BspHI T'CATG_A
Cuts at: 3404 5134 3404
Size: 1730 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2870 844
Size: 2026 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2030 2512 3402 1394
Size: 429 207 482 890 3507
Fragments arranged by size:
3507 890 482 429 207
BsrDI GCAATG_nn'
Cuts at: 65 1248 4010 4184 65
Size: 1183 2762 174 1396
Fragments arranged by size:
2762 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4159 4346 1700
Size: 110 2349 187 2869
Fragments arranged by size:
2869 2349 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5515
BssHII G'CGCG_C
Cuts at: 1925 2145 1925
Size: 220 5295
BssSI C'ACGA_G
Cuts at: 3575 4587 3575
Size: 1012 4503
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2393 794
Size: 1599 3916
BstBI TT'CG_AA
Cuts at: 4319 4319
Size: 5515
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5515
BstYI r'GATC_y
Cuts at: 1972 2845 3212 3597 3614 5066 5152 5164 5510
Size: 873 367 385 17 1452 86 12 346
Cuts at: 5510 1972
Size: 1977
Fragments arranged by size:
1977 1452 873 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2090 2090
Size: 5515
BtgI C'CryG_G
Cuts at: 513 1065 2585 513
Size: 552 1520 3443
Fragments arranged by size:
3443 1520 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4776
Fragments arranged by size:
4776 683 56
BtsI GCAGTG_nn'
Cuts at: 2202 2394 2567 3031 3825 3845 2202
Size: 192 173 464 794 20 3872
Fragments arranged by size:
3872 794 464 192 173 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5480
DraI TTT'AAA
Cuts at: 2819 2819
Size: 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2738 4522 809
Size: 507 1422 1784 1802
Fragments arranged by size:
1802 1784 1422 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2104 2112 2319
Size: 54 1425 50 275 18 274 8 207
Cuts at: 2319 2351 2406 2962 3851 5480 8
Size: 32 55 556 889 1629 43
Fragments arranged by size:
1629 1425 889 556 275 274 207 55
54 50 43 32 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 2319 2351 2962 1537
Size: 275 507 32 611 4090
Fragments arranged by size:
4090 611 507 275 32
EarI CTCTTCn'nnn_
Cuts at: 1353 2283 3445 1353
Size: 930 1162 3423
Fragments arranged by size:
3423 1162 930
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4102 4476 4622 387
Size: 1064 138 2513 374 146 1280
Fragments arranged by size:
2513 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2447 3578 4175 4962 5230 1239
Size: 393 815 1131 597 787 268 1524
Fragments arranged by size:
1524 1131 815 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2462 2462
Size: 5515
EcoO109I rG'GnC_Cy
Cuts at: 2506 2607 2506
Size: 101 5414
EcoRI G'AATT_C
Cuts at: 2663 2678 2824 3202 2663
Size: 15 146 378 4976
Fragments arranged by size:
4976 378 146 15
FspI TGC'GCA
Cuts at: 4021 4021
Size: 5515
HaeII r_GCGC'y
Cuts at: 1678 2202 2804 2810 4662 1678
Size: 524 602 6 1852 2531
Fragments arranged by size:
2531 1852 602 524 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4156 4188 4230 4262 1373
Size: 32 135 32 2584 32 42 32 2626
Fragments arranged by size:
2626 2584 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2674 2859 3105 669
Size: 988 281 736 185 246 3079
Fragments arranged by size:
3079 988 736 281 246 185
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5515
HpaI GTT'AAC
Cuts at: 3105 3105
Size: 5515
KasI G'GCGC_C
Cuts at: 2198 2198
Size: 5515
KpnI G_GTAC'C
Cuts at: 2840 2840
Size: 5515
MfeI C'AATT_G
Cuts at: 3114 3114
Size: 5515
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2109 2634 4629 4813 1305
Size: 804 525 1995 184 2007
Fragments arranged by size:
2007 1995 804 525 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3693
Fragments arranged by size:
3693 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 3473 3832 3991 518
Size: 648 1362 945 359 159 2042
Fragments arranged by size:
2042 1362 945 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2324 2931 3608 4756 5001 1606
Size: 718 607 677 1148 245 2120
Fragments arranged by size:
2120 1148 718 677 607 245
NarI GG'CG_CC
Cuts at: 2199 2199
Size: 5515
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4963
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5515
NotI GC'GGCC_GC
Cuts at: 2319 2962 2319
Size: 643 4872
NspI r_CATG'y
Cuts at: 1132 1229 2873 4418 1132
Size: 97 1644 1545 2229
Fragments arranged by size:
2229 1644 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2154 1348
Size: 806 4709
PciI A'CATG_T
Cuts at: 4414 4414
Size: 5515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5515
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 5097 5129 3584
Size: 32 1481 32 3970
Fragments arranged by size:
3970 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5515
PsiI TTA'TAA
Cuts at: 3085 3085
Size: 5515
PspOMI G'GGCC_C
Cuts at: 2506 2607 2506
Size: 101 5414
PstI C_TGCA'G
Cuts at: 830 2061 2262 2389 2867 830
Size: 1231 201 127 478 3478
Fragments arranged by size:
3478 1231 478 201 127
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5515
SalI G'TCGA_C
Cuts at: 1936 2857 1936
Size: 921 4594
SapI GCTCTTCn'nnn_
Cuts at: 2283 2283
Size: 5515
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 3763 5475 3763
Size: 1712 3803
SfcI C'TryA_G
Cuts at: 826 1045 2023 2057 2258 2385 2863 3998 4679
Size: 219 978 34 201 127 478 1135 681
Cuts at: 4679 4870 826
Size: 191 1471
Fragments arranged by size:
1471 1135 978 681 478 219 201 191
127 34
SfoI GGC'GCC
Cuts at: 2200 2200
Size: 5515
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2601 2842 1872
Size: 729 241 4545
Fragments arranged by size:
4545 729 241
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3619 4520 4782 820
Size: 197 926 1676 901 262 1553
Fragments arranged by size:
1676 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3306 493
Size: 2813 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2873 1132
Size: 1741 3774
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5515
StyI C'CwwG_G
Cuts at: 513 1065 2502 2954 513
Size: 552 1437 452 3074
Fragments arranged by size:
3074 1437 552 452
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3542 3559 3712 3897 4342 325
Size: 1255 1962 17 153 185 445 1498
Fragments arranged by size:
1962 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3761 5473 96
Size: 274 80 33 51 1443 1784 1712 138
Fragments arranged by size:
1784 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5475 5507 5475
Size: 32 5483
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5515
XbaI T'CTAG_A
Cuts at: 2851 2851
Size: 5515
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5515
XmaI C'CCGG_G
Cuts at: 1870 2599 2840 1870
Size: 729 241 4545
Fragments arranged by size:
4545 729 241
XmnI GAAnn'nnTTC
Cuts at: 2663 3644 2663
Size: 981 4534
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SrfI SwaI XcmI