################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AhdI AocI Aor51HI ApaI AscI Asp718I AspEI AspI AvaI AviII BbeI BbrPI BciVI BclI BglI BsaHI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BssHII Bst1107I BstAPI BstZ17I Bsu36I CfoI ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I KasI KpnI KspI MluI MroI NaeI NarI NgoMIV NotI NruI NsiI PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccII 2731 CGCG CG^CG AflII 5679 CTTAAG C^TTAAG AgeI 3326 ACCGGT A^CCGGT Alw44I 399 GTGCAC G^TGCAC ApaLI 399 GTGCAC G^TGCAC AseI 4990 ATTAAT AT^TAAT AsnI 4990 ATTAAT AT^TAAT AvrII 618 CCTAGG C^CTAGG BanI 3586 GGTGCC G^G[CT][GA]CC BcgI 4071 CGAATGTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 202 CCCGG CC^[GC]GG BfrI 5679 CTTAAG C^TTAAG BlnI 618 CCTAGG C^CTAGG BsaAI 2984 TACGTG [CT]AC^GT[GA] BsaBI 2532 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 3345 GTGCAG GTGCAG(16/14) Bsp1407I 5228 TGTACA T^GTACA BsrGI 5228 TGTACA T^GTACA BssSI 1023 CACGAG CACGAG(-5/-1) BstUI 2731 CGCG CG^CG BstXI 2098 CCACCTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 2232 TTTAAA TTT^AAA DrdI 2087 GACAAAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FauI 441 CCCGC CCCGC(4/6) HpaI 3469 GTTAAC GTT^AAC MamI 2532 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 2731 CGCG CG^CG NciI 202 CCCGG CC^[GC]GG NheI 5076 GCTAGC G^CTAGC PinAI 3326 ACCGGT A^CCGGT PshBI 4990 ATTAAT AT^TAAT ScaI 5275 AGTACT AGT^ACT SexAI 2834 ACCTGGT A^CC[AT]GGT SspBI 5228 TGTACA T^GTACA XbaI 3871 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 1587 GTCTAC GT^[AC][GT]AC 4228 GTAGAC BalI 2512 TGGCCA TGG^CCA 2585 TGGCCA BbvCI 1309 GCTGAGG CCTCAGC(-5/-2) 3274 GCTGAGG BlpI 1901 GCTCAGC GC^T[ACGT]AGC 3576 GCTCAGC Bpu1102I 1901 GCTCAGC GC^T[ACGT]AGC 3576 GCTCAGC BsaWI 3326 ACCGGT [AT]^CCGG[AT] 5468 ACCGGA BseRI 3336 CTCCTC GAGGAG(10/8) 5399 CTCCTC BspMI 309 ACCTGC ACCTGC(4/8) 3517 ACCTGC BsrFI 1836 GCCGGT [GA]^CCGG[CT] 3326 ACCGGT BstBI 638 TTCGAA TT^CGAA 827 TTCGAA CelII 1901 GCTCAGC GC^T[ACGT]AGC 3576 GCTCAGC Cfr10I 1836 GCCGGT [GA]^CCGG[CT] 3326 ACCGGT DraII 21 AGGACCT [GA]G^G[ACGT]CC[CT] 1819 AGGTCCC EcoO109I 21 AGGACCT [GA]G^G[ACGT]CC[CT] 1819 AGGTCCC HincII 3469 GTTAAC GT[CT]^[GA]AC 4586 GTCAAC HindII 3469 GTTAAC GT[CT]^[GA]AC 4586 GTCAAC MluNI 2512 TGGCCA TGG^CCA 2585 TGGCCA MscI 2512 TGGCCA TGG^CCA 2585 TGGCCA NspV 638 TTCGAA TT^CGAA 827 TTCGAA PflMI 3731 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5164 CCATCTGTTGG PpuMI 21 AGGACCT [GA]G^G[AT]CC[CT] 1819 AGGTCCC SapI 634 GCTCTTC GCTCTTC(1/4) 4917 GCTCTTC SfuI 638 TTCGAA TT^CGAA 827 TTCGAA SphI 386 GCATGC GCATG^C 2618 GCATGC StuI 687 AGGCCT AGG^CCT 3422 AGGCCT Van91I 3731 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5164 CCATCTGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BamHI BanII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BspLU11I BsrDI BsrI BssKI BstEII BstF5I BstNI BstPI BstYI Cac8I Cfr13I DdeI DpnII DsaI EaeI EarI EcoNI EcoO65I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MwoI NcoI NdeI NdeII NlaIII NlaIV NspI PleI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI