(Circular) (Six-base) MAPSORT of: pFN21AE2325.seq Check: 7103 from: 1 to: 10636 pFN21AE2325 10636 bp With 182 enzymes: SgfI * June 20, 2011 14:14 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2323 2323 Size: 10636 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8446 278 Size: 53 83 186 936 6910 2468 Fragments arranged by size: 6910 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3593 6234 7979 1937 Size: 1656 2641 1745 4594 Fragments arranged by size: 4594 2641 1745 1656 Acc65I G'GTAC_C Cuts at: 7957 7957 Size: 10636 AclI AA'CG_TT Cuts at: 1563 8763 9136 1563 Size: 7200 373 3063 Fragments arranged by size: 7200 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2717 2837 6624 7293 8699 10083 10351 1632 Size: 1085 120 3787 669 1406 1384 268 1917 Fragments arranged by size: 3787 1917 1406 1384 1085 669 268 120 AflII C'TTAA_G Cuts at: 820 1017 7684 820 Size: 197 6667 3772 Fragments arranged by size: 6667 3772 197 AflIII A'CryG_T Cuts at: 1203 6267 7002 7230 9535 10512 1203 Size: 5064 735 228 2305 977 1327 Fragments arranged by size: 5064 2305 1327 977 735 228 AgeI A'CCGG_T Cuts at: 5331 9467 5331 Size: 4136 6500 AhdI GACnn_n'nnGTC Cuts at: 9365 9365 Size: 10636 AleI CACnn'nnGTG Cuts at: 1166 2993 3552 4108 4583 1166 Size: 1827 559 556 475 7219 Fragments arranged by size: 7219 1827 559 556 475 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2195 1184 Size: 1011 9625 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4452 4484 7553 7585 4452 Size: 32 3069 32 7503 Fragments arranged by size: 7503 3069 32 32 AlwNI CAG_nnn'CTG Cuts at: 3115 3338 3937 5399 8379 9951 3115 Size: 223 599 1462 2980 1572 3800 Fragments arranged by size: 3800 2980 1572 1462 599 223 ApaLI G'TGCA_C Cuts at: 2404 8693 9849 2404 Size: 6289 1156 3191 Fragments arranged by size: 6289 3191 1156 ApoI r'AATT_y Cuts at: 1493 2543 2612 2836 3269 3710 4130 4166 4283 Size: 1050 69 224 433 441 420 36 117 Cuts at: 4283 4309 4994 5410 5992 7255 7945 8171 8323 Size: 26 685 416 582 1263 690 226 152 Cuts at: 8323 10432 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1263 1050 690 685 582 441 433 420 416 226 224 152 117 69 36 26 AseI AT'TA_AT Cuts at: 160 1032 6996 9190 160 Size: 872 5964 2194 1606 Fragments arranged by size: 5964 2194 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10636 AvaI C'yCGr_G Cuts at: 1870 1943 7961 1870 Size: 73 6018 4545 Fragments arranged by size: 6018 4545 73 AvrII C'CTAG_G Cuts at: 2623 2623 Size: 10636 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2977 3010 2977 Size: 33 10603 BamHI G'GATC_C Cuts at: 2927 4198 4366 6858 7399 7476 7966 8333 2927 Size: 1271 168 2492 541 77 490 367 5230 Fragments arranged by size: 5230 2492 1271 541 490 367 168 77 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5591 7957 9412 618 Size: 325 217 627 3804 2366 1455 1842 Fragments arranged by size: 3804 2366 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2226 3173 3413 3764 4440 4567 4880 7955 Size: 835 947 240 351 676 127 313 3075 Cuts at: 7955 1391 Size: 4072 Fragments arranged by size: 4072 3075 947 835 676 351 313 240 127 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2705 2901 4489 4594 4920 5193 7908 Size: 971 806 196 1588 105 326 273 2715 Cuts at: 7908 928 Size: 3656 Fragments arranged by size: 3656 2715 1588 971 806 326 273 196 105 BbvCI CC'TCA_GC Cuts at: 3315 5280 3315 Size: 1965 8671 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6065 6099 8827 8861 1903 Size: 34 4128 34 2728 34 3678 Fragments arranged by size: 4128 3678 2728 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8528 9738 8528 Size: 1210 9426 BclI T'GATC_A Cuts at: 1548 1548 Size: 10636 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 9247 136 Size: 107 122 71 631 605 7575 1525 Fragments arranged by size: 7575 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4210 5056 5664 5815 10631 4210 Size: 846 608 151 4816 4215 Fragments arranged by size: 4816 4215 846 608 151 BlpI GC'TnA_GC Cuts at: 3907 5582 8053 3907 Size: 1675 2471 6490 Fragments arranged by size: 6490 2471 1675 Bme1580I G_kGCm'C Cuts at: 1790 2408 3777 3950 5596 8697 9853 1790 Size: 618 1369 173 1646 3101 1156 2573 Fragments arranged by size: 3101 2573 1646 1369 1156 618 173 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10636 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3100 7348 8367 Size: 416 53 83 399 24 1683 4248 1019 Cuts at: 8367 9325 442 Size: 958 1753 Fragments arranged by size: 4248 1753 1683 1019 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 7085 1056 Size: 6029 4607 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 3454 4919 5397 5669 6669 7271 9296 Size: 1010 1205 1465 478 272 1000 602 2025 Cuts at: 9296 1239 Size: 2579 Fragments arranged by size: 2579 2025 1465 1205 1010 1000 602 478 272 Bpu10I CC'TnA_GC Cuts at: 3315 5280 3315 Size: 1965 8671 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5114 7777 8761 9626 9924 5114 Size: 2663 984 865 298 5826 Fragments arranged by size: 5826 2663 984 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3892 4754 7010 9299 882 Size: 617 2393 862 2256 2289 2219 Fragments arranged by size: 2393 2289 2256 2219 862 617 BsaAI yAC'GTr Cuts at: 493 1204 4991 8427 493 Size: 711 3787 3436 2702 Fragments arranged by size: 3787 3436 2702 711 BsaBI GATnn'nnATC Cuts at: 4541 10630 4541 Size: 6089 4547 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 8443 8825 9460 275 Size: 53 83 186 936 6910 382 635 1451 Fragments arranged by size: 6910 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5331 7473 9069 9467 9741 9888 10299 5331 Size: 2142 1596 398 274 147 411 5668 Fragments arranged by size: 5668 2142 1596 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 6642 6672 7816 7846 1178 Size: 30 165 30 5239 30 1144 30 3968 Fragments arranged by size: 5239 3968 1144 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 5332 7395 1186 Size: 4146 2063 4427 Fragments arranged by size: 4427 4146 2063 BseYI C'CCAG_C Cuts at: 3048 5219 6433 7068 9839 10335 3048 Size: 2171 1214 635 2771 496 3349 Fragments arranged by size: 3349 2771 2171 1214 635 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5371 1163 Size: 573 3635 6428 Fragments arranged by size: 6428 3635 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8086 8847 9875 1540 Size: 275 186 6085 761 1028 2301 Fragments arranged by size: 6085 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2408 3413 3577 3729 4440 4880 8697 8782 9853 Size: 1005 164 152 711 440 3817 85 1071 Cuts at: 9853 2408 Size: 3191 Fragments arranged by size: 3817 3191 1071 1005 711 440 164 152 85 BsmI GAATG_Cn' Cuts at: 3618 5281 5911 8147 8240 3618 Size: 1663 630 2236 93 6014 Fragments arranged by size: 6014 2236 1663 630 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2408 3173 3413 3577 3729 3764 Size: 399 436 182 765 240 164 152 35 Cuts at: 3764 3777 3950 4440 4567 4880 5596 7955 8697 Size: 13 173 490 127 313 716 2359 742 Cuts at: 8697 8782 9853 1391 Size: 85 1071 2174 Fragments arranged by size: 2359 2174 1071 765 742 716 490 436 399 313 240 182 173 164 152 127 85 35 13 BspEI T'CCGG_A Cuts at: 10299 10299 Size: 10636 BspHI T'CATG_A Cuts at: 2748 5571 5934 8525 10255 2748 Size: 2823 363 2591 1730 3129 Fragments arranged by size: 3129 2823 2591 1730 363 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2323 5531 7991 844 Size: 1479 3208 2460 3489 Fragments arranged by size: 3489 3208 2460 1479 BsrBI CCG'CTC Cuts at: 1394 1823 8523 1394 Size: 429 6700 3507 Fragments arranged by size: 6700 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2016 2082 2635 4044 6429 7236 7440 Size: 1183 768 66 553 1409 2385 807 204 Cuts at: 7440 9131 9305 65 Size: 1691 174 1396 Fragments arranged by size: 2385 1691 1409 1396 1183 807 768 553 204 174 66 BsrFI r'CCGG_y Cuts at: 1700 1810 3841 5331 9280 9467 1700 Size: 110 2031 1490 3949 187 2869 Fragments arranged by size: 3949 2869 2031 1490 187 110 BsrGI T'GTAC_A Cuts at: 96 7233 96 Size: 7137 3499 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10636 BssSI C'ACGA_G Cuts at: 3028 8696 9708 3028 Size: 5668 1012 3956 Fragments arranged by size: 5668 3956 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 10636 BstBI TT'CG_AA Cuts at: 2644 2833 9440 2644 Size: 189 6607 3840 Fragments arranged by size: 6607 3840 189 BstEII G'GTnAC_C Cuts at: 1184 4571 6155 6559 7163 7469 1184 Size: 3387 1584 404 604 306 4351 Fragments arranged by size: 4351 3387 1584 604 404 306 BstXI CCAn_nnnn'nTGG Cuts at: 4110 4110 Size: 10636 BstYI r'GATC_y Cuts at: 1972 2927 3698 4198 4210 4366 5056 5664 5815 Size: 955 771 500 12 156 690 608 151 Cuts at: 5815 6858 7399 7450 7476 7966 8333 8718 8735 Size: 1043 541 51 26 490 367 385 17 Cuts at: 8735 10187 10273 10285 10631 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1043 955 771 690 608 541 500 490 385 367 346 156 151 86 51 26 17 12 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2564 3210 4362 4688 7446 7458 Size: 552 939 560 646 1152 326 2758 12 Cuts at: 7458 513 Size: 3691 Fragments arranged by size: 3691 2758 1152 939 646 560 552 326 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 6127 487 Size: 683 56 4901 4996 Fragments arranged by size: 4996 4901 683 56 BtsI GCAGTG_nn' Cuts at: 2161 4950 5299 5593 6222 8152 8946 8966 2161 Size: 2789 349 294 629 1930 794 20 3831 Fragments arranged by size: 3831 2789 1930 794 629 349 294 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10636 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4050 4085 529 Size: 35 3486 35 7080 Fragments arranged by size: 7080 3486 35 35 DraI TTT'AAA Cuts at: 4239 7940 4239 Size: 3701 6935 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4098 9643 809 Size: 507 2782 5545 1802 Fragments arranged by size: 5545 2782 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4517 4590 6823 Size: 54 1425 50 275 18 2687 73 2233 Cuts at: 6823 8083 8972 10601 8 Size: 1260 889 1629 43 Fragments arranged by size: 2687 2233 1629 1425 1260 889 275 73 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8083 1537 Size: 275 6271 4090 Fragments arranged by size: 6271 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2646 3682 4077 6929 7413 7551 8566 1353 Size: 1293 1036 395 2852 484 138 1015 3423 Fragments arranged by size: 3423 2852 1293 1036 1015 484 395 138 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 9223 9597 9743 387 Size: 1064 138 7634 374 146 1280 Fragments arranged by size: 7634 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3411 4438 4878 3411 Size: 1027 440 9169 Fragments arranged by size: 9169 1027 440 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2717 2837 3454 4919 5397 5669 Size: 393 617 468 120 617 1465 478 272 Cuts at: 5669 6624 6669 7271 7293 8699 9296 10083 10351 Size: 955 45 602 22 1406 597 787 268 Cuts at: 10351 1239 Size: 1524 Fragments arranged by size: 1524 1465 1406 955 787 617 617 602 597 478 468 393 272 268 120 45 22 EcoNI CCTnn'n_nnAGG Cuts at: 2022 3067 3998 5379 6468 2022 Size: 1045 931 1381 1089 6190 Fragments arranged by size: 6190 1381 1089 1045 931 EcoO109I rG'GnC_Cy Cuts at: 2027 3825 2027 Size: 1798 8838 EcoRI G'AATT_C Cuts at: 2543 4283 5410 7945 8323 2543 Size: 1740 1127 2535 378 4856 Fragments arranged by size: 4856 2535 1740 1127 378 FspI TGC'GCA Cuts at: 9142 9142 Size: 10636 HaeII r_GCGC'y Cuts at: 1678 9783 1678 Size: 8105 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3488 3520 3683 3715 3890 Size: 32 135 32 1916 32 163 32 175 Cuts at: 3890 3922 5101 5133 5186 5218 7468 7500 7528 Size: 32 1179 32 53 32 2250 32 28 Cuts at: 7528 7560 7628 7660 9277 9309 9351 9383 1373 Size: 32 68 32 1617 32 42 32 2626 Fragments arranged by size: 2626 2250 1916 1617 1179 175 163 135 68 53 42 32 32 32 32 32 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 5476 6593 7980 8226 669 Size: 988 281 3538 1117 1387 246 3079 Fragments arranged by size: 3538 3079 1387 1117 988 281 246 HindIII A'AGCT_T Cuts at: 748 2735 5557 6368 748 Size: 1987 2822 811 5016 Fragments arranged by size: 5016 2822 1987 811 HpaI GTT'AAC Cuts at: 5476 8226 5476 Size: 2750 7886 KpnI G_GTAC'C Cuts at: 7961 7961 Size: 10636 MfeI C'AATT_G Cuts at: 2703 3321 4905 5241 8235 2703 Size: 618 1584 336 2994 5104 Fragments arranged by size: 5104 2994 1584 618 336 MluI A'CGCG_T Cuts at: 10512 10512 Size: 10636 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6127 6987 7156 9750 9934 1305 Size: 4822 860 169 2594 184 2007 Fragments arranged by size: 4822 2594 2007 860 184 169 MscI TGG'CCA Cuts at: 10 64 1489 1832 4519 4592 10 Size: 54 1425 343 2687 73 6054 Fragments arranged by size: 6054 2687 1425 343 73 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2429 2993 3552 4108 4583 5939 Size: 648 843 420 564 559 556 475 1356 Cuts at: 5939 6389 7445 8594 8953 9112 518 Size: 450 1056 1149 359 159 2042 Fragments arranged by size: 2042 1356 1149 1056 843 648 564 559 556 475 450 420 359 159 MspA1I CmG'CkG Cuts at: 1606 2800 3048 3329 3335 4337 7391 8052 8729 Size: 1194 248 281 6 1002 3054 661 677 Cuts at: 8729 9877 10122 1606 Size: 1148 245 2120 Fragments arranged by size: 3054 2120 1194 1148 1002 677 661 281 248 245 6 NcoI C'CATG_G Cuts at: 513 1065 2004 3210 4362 7446 7458 513 Size: 552 939 1206 1152 3084 12 3691 Fragments arranged by size: 3691 3084 1206 1152 939 552 12 NdeI CA'TA_TG Cuts at: 387 3217 4372 5713 6597 7843 387 Size: 2830 1155 1341 884 1246 3180 Fragments arranged by size: 3180 2830 1341 1246 1155 884 NheI G'CTAG_C Cuts at: 1052 7081 1052 Size: 6029 4607 NotI GC'GGCC_GC Cuts at: 8083 8083 Size: 10636 NspI r_CATG'y Cuts at: 1132 1229 2395 4534 4627 6271 7006 7234 7994 Size: 97 1166 2139 93 1644 735 228 760 Cuts at: 7994 9539 1132 Size: 1545 2229 Fragments arranged by size: 2229 2139 1644 1545 1166 760 735 228 97 93 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10636 PciI A'CATG_T Cuts at: 6267 7002 7230 9535 6267 Size: 735 228 2305 7368 Fragments arranged by size: 7368 2305 735 228 PflMI CCAn_nnn'nTGG Cuts at: 1108 5742 7175 1108 Size: 4634 1433 4569 Fragments arranged by size: 4634 4569 1433 PfoI T'CCnGG_A Cuts at: 5646 7135 5646 Size: 1489 9147 PmeI GTTT'AAAC Cuts at: 7940 7940 Size: 10636 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8705 8737 10218 10250 8705 Size: 32 1481 32 9091 Fragments arranged by size: 9091 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2027 3825 2027 Size: 1798 8838 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10636 PsiI TTA'TAA Cuts at: 5721 6635 8206 5721 Size: 914 1571 8151 Fragments arranged by size: 8151 1571 914 PstI C_TGCA'G Cuts at: 830 7988 830 Size: 7158 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10636 PvuII CAG'CTG Cuts at: 2800 3048 4337 7391 2800 Size: 248 1289 3054 6045 Fragments arranged by size: 6045 3054 1289 248 SacI G_AGCT'C Cuts at: 3413 4440 4880 3413 Size: 1027 440 9169 Fragments arranged by size: 9169 1027 440 SalI G'TCGA_C Cuts at: 1936 7978 1936 Size: 6042 4594 SapI GCTCTTCn'nnn_ Cuts at: 2646 6929 2646 Size: 4283 6353 SbfI CC_TGCA'GG Cuts at: 7988 7988 Size: 10636 ScaI AGT'ACT Cuts at: 7282 8884 10596 7282 Size: 1602 1712 7322 Fragments arranged by size: 7322 1712 1602 SexAI A'CCwGG_T Cuts at: 4839 4839 Size: 10636 SfcI C'TryA_G Cuts at: 826 1045 2795 4302 6696 7984 9119 9800 9991 Size: 219 1750 1507 2394 1288 1135 681 191 Cuts at: 9991 826 Size: 1471 Fragments arranged by size: 2394 1750 1507 1471 1288 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10636 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10636 SmaI CCC'GGG Cuts at: 1872 7963 1872 Size: 6091 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5129 7684 7792 8740 9641 9903 Size: 197 926 3186 2555 108 948 901 262 Cuts at: 9903 820 Size: 1553 Fragments arranged by size: 3186 2555 1553 948 926 901 262 197 108 SnaBI TAC'GTA Cuts at: 493 8427 493 Size: 7934 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10636 SphI G_CATG'C Cuts at: 1132 2395 4627 7994 1132 Size: 1263 2232 3367 3774 Fragments arranged by size: 3774 3367 2232 1263 SspI AAT'ATT Cuts at: 5 52 2909 4399 6709 7182 8560 5 Size: 47 2857 1490 2310 473 1378 2081 Fragments arranged by size: 2857 2310 2081 1490 1378 473 47 StuI AGG'CCT Cuts at: 1517 2694 5429 1517 Size: 1177 2735 6724 Fragments arranged by size: 6724 2735 1177 StyI C'CwwG_G Cuts at: 513 1065 2004 2623 3210 3550 4106 4362 6484 Size: 552 939 619 587 340 556 256 2122 Cuts at: 6484 7446 7458 8075 513 Size: 962 12 617 3074 Fragments arranged by size: 3074 2122 962 939 619 617 587 556 552 340 256 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4284 7511 8663 8680 8833 9018 9463 Size: 1255 2704 3227 1152 17 153 185 445 Cuts at: 9463 325 Size: 1498 Fragments arranged by size: 3227 2704 1498 1255 1152 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2374 2472 4294 Size: 274 80 33 51 1443 397 98 1822 Cuts at: 4294 7233 7280 8882 10594 96 Size: 2939 47 1602 1712 138 Fragments arranged by size: 2939 1822 1712 1602 1443 397 274 138 98 80 51 47 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4827 4859 5151 5183 6642 6674 7816 7848 10596 Size: 32 292 32 1459 32 1142 32 2748 Cuts at: 10596 10628 4827 Size: 32 4835 Fragments arranged by size: 4835 2748 1459 1142 292 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10636 XbaI T'CTAG_A Cuts at: 5876 7972 5876 Size: 2096 8540 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10636 XmaI C'CCGG_G Cuts at: 1870 7961 1870 Size: 6091 4545 XmnI GAAnn'nnTTC Cuts at: 4706 4998 5694 6323 8765 4706 Size: 292 696 629 2442 6577 Fragments arranged by size: 6577 2442 696 629 292 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8444 276 Size: 53 83 186 936 6910 2468 Fragments arranged by size: 6910 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI