Restriction map for
pFN21AE2325
(Linear) (Six-base) MAPSORT of: KIEE2325.seq Check: 3199 from: 1 to: 5931
KIEE2325.orf
With 182 enzymes: SgfI *
June 20, 2011 13:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 318 5931
Size: 318 5613
AccI GT'mk_AC
Cuts at: 0 1588 4229 5931
Size: 1588 2641 1702
Fragments arranged by size:
2641 1702 1588
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 832 4619 5288 5931
Size: 712 120 3787 669 643
Fragments arranged by size:
3787 712 669 643 120
AflII C'TTAA_G
Cuts at: 0 5679 5931
Size: 5679 252
AflIII A'CryG_T
Cuts at: 0 4262 4997 5225 5931
Size: 4262 735 228 706
Fragments arranged by size:
4262 735 706 228
AgeI A'CCGG_T
Cuts at: 0 3326 5931
Size: 3326 2605
AleI CACnn'nnGTG
Cuts at: 0 988 1547 2103 2578 5931
Size: 988 559 556 475 3353
Fragments arranged by size:
3353 988 559 556 475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 190 5931
Size: 190 5741
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2447 2479 5548 5580 5931
Size: 2447 32 3069 32 351
Fragments arranged by size:
3069 2447 351 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1110 1333 1932 3394 5931
Size: 1110 223 599 1462 2537
Fragments arranged by size:
2537 1462 1110 599 223
ApaLI G'TGCA_C
Cuts at: 0 399 5931
Size: 399 5532
ApoI r'AATT_y
Cuts at: 0 538 607 831 1264 1705 2125 2161 2278
Size: 538 69 224 433 441 420 36 117
Cuts at: 2278 2304 2989 3405 3987 5250 5931
Size: 26 685 416 582 1263 681
Fragments arranged by size:
1263 685 681 582 538 441 433 420
416 224 117 69 36 26
AseI AT'TA_AT
Cuts at: 0 4991 5931
Size: 4991 940
AvrII C'CTAG_G
Cuts at: 0 618 5931
Size: 618 5313
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 972 1005 5931
Size: 972 33 4926
Fragments arranged by size:
4926 972 33
BamHI G'GATC_C
Cuts at: 0 922 2193 2361 4853 5394 5471 5931
Size: 922 1271 168 2492 541 77 460
Fragments arranged by size:
2492 1271 922 541 460 168 77
BanI G'GyrC_C
Cuts at: 0 3586 5931
Size: 3586 2345
BanII G_rGCy'C
Cuts at: 0 221 1168 1408 1759 2435 2562 2875 5931
Size: 221 947 240 351 676 127 313 3056
Fragments arranged by size:
3056 947 676 351 313 240 221 127
BbsI GAAGACnn'nnnn_
Cuts at: 0 700 896 2484 2589 2915 3188 5903 5931
Size: 700 196 1588 105 326 273 2715 28
Fragments arranged by size:
2715 1588 700 326 273 196 105 28
BbvCI CC'TCA_GC
Cuts at: 0 1310 3275 5931
Size: 1310 1965 2656
Fragments arranged by size:
2656 1965 1310
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4060 4094 5931
Size: 4060 34 1837
Fragments arranged by size:
4060 1837 34
BglII A'GATC_T
Cuts at: 0 2205 3051 3659 3810 5931
Size: 2205 846 608 151 2121
Fragments arranged by size:
2205 2121 846 608 151
BlpI GC'TnA_GC
Cuts at: 0 1902 3577 5931
Size: 1902 1675 2354
Fragments arranged by size:
2354 1902 1675
Bme1580I G_kGCm'C
Cuts at: 0 403 1772 1945 3591 5931
Size: 403 1369 173 1646 2340
Fragments arranged by size:
2340 1646 1369 403 173
BmrI ACTGGGnnnn_n'
Cuts at: 0 1095 5343 5931
Size: 1095 4248 588
Fragments arranged by size:
4248 1095 588
BmtI G_CTAG'C
Cuts at: 0 5080 5931
Size: 5080 851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 1449 2914 3392 3664 4664 5266 5931
Size: 244 1205 1465 478 272 1000 602 665
Fragments arranged by size:
1465 1205 1000 665 602 478 272 244
Bpu10I CC'TnA_GC
Cuts at: 0 1310 3275 5931
Size: 1310 1965 2656
Fragments arranged by size:
2656 1965 1310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3109 5772 5931
Size: 3109 2663 159
Fragments arranged by size:
3109 2663 159
BsaI GGTCTCn'nnnn_
Cuts at: 0 1887 2749 5005 5931
Size: 1887 862 2256 926
Fragments arranged by size:
2256 1887 926 862
BsaAI yAC'GTr
Cuts at: 0 2986 5931
Size: 2986 2945
BsaBI GATnn'nnATC
Cuts at: 0 2536 5931
Size: 2536 3395
BsaWI w'CCGG_w
Cuts at: 0 3326 5468 5931
Size: 3326 2142 463
Fragments arranged by size:
3326 2142 463
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4637 4667 5811 5841 5931
Size: 4637 30 1144 30 90
Fragments arranged by size:
4637 1144 90 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3327 5390 5931
Size: 3327 2063 541
Fragments arranged by size:
3327 2063 541
BseYI C'CCAG_C
Cuts at: 0 1043 3214 4428 5063 5931
Size: 1043 2171 1214 635 868
Fragments arranged by size:
2171 1214 1043 868 635
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3366 5931
Size: 3366 2565
BsiHKAI G_wGCw'C
Cuts at: 0 403 1408 1572 1724 2435 2875 5931
Size: 403 1005 164 152 711 440 3056
Fragments arranged by size:
3056 1005 711 440 403 164 152
BsmI GAATG_Cn'
Cuts at: 0 1613 3276 3906 5931
Size: 1613 1663 630 2025
Fragments arranged by size:
2025 1663 1613 630
Bsp1286I G_dGCh'C
Cuts at: 0 221 403 1168 1408 1572 1724 1759 1772
Size: 221 182 765 240 164 152 35 13
Cuts at: 1772 1945 2435 2562 2875 3591 5931
Size: 173 490 127 313 716 2340
Fragments arranged by size:
2340 765 716 490 313 240 221 182
173 164 152 127 35 13
BspHI T'CATG_A
Cuts at: 0 743 3566 3929 5931
Size: 743 2823 363 2002
Fragments arranged by size:
2823 2002 743 363
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 318 3526 5931
Size: 318 3208 2405
Fragments arranged by size:
3208 2405 318
BsrDI GCAATG_nn'
Cuts at: 0 11 77 630 2039 4424 5231 5435 5931
Size: 11 66 553 1409 2385 807 204 496
Fragments arranged by size:
2385 1409 807 553 496 204 66 11
BsrFI r'CCGG_y
Cuts at: 0 1836 3326 5931
Size: 1836 1490 2605
Fragments arranged by size:
2605 1836 1490
BsrGI T'GTAC_A
Cuts at: 0 5228 5931
Size: 5228 703
BssSI C'ACGA_G
Cuts at: 0 1023 5931
Size: 1023 4908
BstBI TT'CG_AA
Cuts at: 0 639 828 5931
Size: 639 189 5103
Fragments arranged by size:
5103 639 189
BstEII G'GTnAC_C
Cuts at: 0 2566 4150 4554 5158 5464 5931
Size: 2566 1584 404 604 306 467
Fragments arranged by size:
2566 1584 604 467 404 306
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2105 5931
Size: 2105 3826
BstYI r'GATC_y
Cuts at: 0 922 1693 2193 2205 2361 3051 3659 3810
Size: 922 771 500 12 156 690 608 151
Cuts at: 3810 4853 5394 5445 5471 5931
Size: 1043 541 51 26 460
Fragments arranged by size:
1043 922 771 690 608 541 500 460
156 151 51 26 12
BtgI C'CryG_G
Cuts at: 0 559 1205 2357 2683 5441 5453 5931
Size: 559 646 1152 326 2758 12 478
Fragments arranged by size:
2758 1152 646 559 478 326 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4122 5931
Size: 4122 1809
BtsI GCAGTG_nn'
Cuts at: 0 156 2945 3294 3588 4217 5931
Size: 156 2789 349 294 629 1714
Fragments arranged by size:
2789 1714 629 349 294 156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2045 2080 5931
Size: 2045 35 3851
Fragments arranged by size:
3851 2045 35
DraI TTT'AAA
Cuts at: 0 2234 5931
Size: 2234 3697
DrdI GACnn_nn'nnGTC
Cuts at: 0 2093 5931
Size: 2093 3838
EaeI y'GGCC_r
Cuts at: 0 2512 2585 4818 5931
Size: 2512 73 2233 1113
Fragments arranged by size:
2512 2233 1113 73
EarI CTCTTCn'nnn_
Cuts at: 0 641 1677 2072 4924 5408 5546 5931
Size: 641 1036 395 2852 484 138 385
Fragments arranged by size:
2852 1036 641 484 395 385 138
EcoICRI GAG'CTC
Cuts at: 0 1406 2433 2873 5931
Size: 1406 1027 440 3058
Fragments arranged by size:
3058 1406 1027 440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 712 832 1449 2914 3392 3664 4619
Size: 244 468 120 617 1465 478 272 955
Cuts at: 4619 4664 5266 5288 5931
Size: 45 602 22 643
Fragments arranged by size:
1465 955 643 617 602 478 468 272
244 120 45 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 17 1062 1993 3374 4463 5931
Size: 17 1045 931 1381 1089 1468
Fragments arranged by size:
1468 1381 1089 1045 931 17
EcoO109I rG'GnC_Cy
Cuts at: 0 22 1820 5931
Size: 22 1798 4111
Fragments arranged by size:
4111 1798 22
EcoRI G'AATT_C
Cuts at: 0 538 2278 3405 5931
Size: 538 1740 1127 2526
Fragments arranged by size:
2526 1740 1127 538
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1483 1515 1678 1710 1885 1917 3096 3128
Size: 1483 32 163 32 175 32 1179 32
Cuts at: 3128 3181 3213 5463 5495 5523 5555 5623 5655
Size: 53 32 2250 32 28 32 68 32
Cuts at: 5655 5931
Size: 276
Fragments arranged by size:
2250 1483 1179 276 175 163 68 53
32 32 32 32 32 32 32 32
28
HincII GTy'rAC
Cuts at: 0 3471 4588 5931
Size: 3471 1117 1343
Fragments arranged by size:
3471 1343 1117
HindIII A'AGCT_T
Cuts at: 0 730 3552 4363 5931
Size: 730 2822 811 1568
Fragments arranged by size:
2822 1568 811 730
HpaI GTT'AAC
Cuts at: 0 3471 5931
Size: 3471 2460
MfeI C'AATT_G
Cuts at: 0 698 1316 2900 3236 5931
Size: 698 618 1584 336 2695
Fragments arranged by size:
2695 1584 698 618 336
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 4122 4982 5151 5931
Size: 4122 860 169 780
Fragments arranged by size:
4122 860 780 169
MscI TGG'CCA
Cuts at: 0 2514 2587 5931
Size: 2514 73 3344
Fragments arranged by size:
3344 2514 73
MslI CAynn'nnrTG
Cuts at: 0 424 988 1547 2103 2578 3934 4384 5440
Size: 424 564 559 556 475 1356 450 1056
Cuts at: 5440 5931
Size: 491
Fragments arranged by size:
1356 1056 564 559 556 491 475 450
424
MspA1I CmG'CkG
Cuts at: 0 795 1043 1324 1330 2332 5386 5931
Size: 795 248 281 6 1002 3054 545
Fragments arranged by size:
3054 1002 795 545 281 248 6
NcoI C'CATG_G
Cuts at: 0 1205 2357 5441 5453 5931
Size: 1205 1152 3084 12 478
Fragments arranged by size:
3084 1205 1152 478 12
NdeI CA'TA_TG
Cuts at: 0 1212 2367 3708 4592 5838 5931
Size: 1212 1155 1341 884 1246 93
Fragments arranged by size:
1341 1246 1212 1155 884 93
NheI G'CTAG_C
Cuts at: 0 5076 5931
Size: 5076 855
NspI r_CATG'y
Cuts at: 0 390 2529 2622 4266 5001 5229 5931
Size: 390 2139 93 1644 735 228 702
Fragments arranged by size:
2139 1644 735 702 390 228 93
PciI A'CATG_T
Cuts at: 0 4262 4997 5225 5931
Size: 4262 735 228 706
Fragments arranged by size:
4262 735 706 228
PflMI CCAn_nnn'nTGG
Cuts at: 0 3737 5170 5931
Size: 3737 1433 761
Fragments arranged by size:
3737 1433 761
PfoI T'CCnGG_A
Cuts at: 0 3641 5130 5931
Size: 3641 1489 801
Fragments arranged by size:
3641 1489 801
PpuMI rG'GwC_Cy
Cuts at: 0 22 1820 5931
Size: 22 1798 4111
Fragments arranged by size:
4111 1798 22
PsiI TTA'TAA
Cuts at: 0 3716 4630 5931
Size: 3716 914 1301
Fragments arranged by size:
3716 1301 914
PvuII CAG'CTG
Cuts at: 0 795 1043 2332 5386 5931
Size: 795 248 1289 3054 545
Fragments arranged by size:
3054 1289 795 545 248
SacI G_AGCT'C
Cuts at: 0 1408 2435 2875 5931
Size: 1408 1027 440 3056
Fragments arranged by size:
3056 1408 1027 440
SapI GCTCTTCn'nnn_
Cuts at: 0 641 4924 5931
Size: 641 4283 1007
Fragments arranged by size:
4283 1007 641
ScaI AGT'ACT
Cuts at: 0 5277 5931
Size: 5277 654
SexAI A'CCwGG_T
Cuts at: 0 2834 5931
Size: 2834 3097
SfcI C'TryA_G
Cuts at: 0 790 2297 4691 5931
Size: 790 1507 2394 1240
Fragments arranged by size:
2394 1507 1240 790
SmlI C'TyrA_G
Cuts at: 0 3124 5679 5787 5931
Size: 3124 2555 108 144
Fragments arranged by size:
3124 2555 144 108
SphI G_CATG'C
Cuts at: 0 390 2622 5931
Size: 390 2232 3309
Fragments arranged by size:
3309 2232 390
SspI AAT'ATT
Cuts at: 0 904 2394 4704 5177 5931
Size: 904 1490 2310 473 754
Fragments arranged by size:
2310 1490 904 754 473
StuI AGG'CCT
Cuts at: 0 689 3424 5931
Size: 689 2735 2507
Fragments arranged by size:
2735 2507 689
StyI C'CwwG_G
Cuts at: 0 618 1205 1545 2101 2357 4479 5441 5453
Size: 618 587 340 556 256 2122 962 12
Cuts at: 5453 5931
Size: 478
Fragments arranged by size:
2122 962 618 587 556 478 340 256
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2279 5506 5931
Size: 2279 3227 425
Fragments arranged by size:
3227 2279 425
TatI w'GTAC_w
Cuts at: 0 369 467 2289 5228 5275 5931
Size: 369 98 1822 2939 47 656
Fragments arranged by size:
2939 1822 656 369 98 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2822 2854 3146 3178 4637 4669 5811 5843
Size: 2822 32 292 32 1459 32 1142 32
Cuts at: 5843 5931
Size: 88
Fragments arranged by size:
2822 1459 1142 292 88 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 3871 5931
Size: 3871 2060
XmnI GAAnn'nnTTC
Cuts at: 0 2701 2993 3689 4318 5931
Size: 2701 292 696 629 1613
Fragments arranged by size:
2701 1613 696 629 292
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AvrII BaeI BamHI
BanI BanII BbsI BbvCI BcgI BglII BlpI Bme1580I
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrGI BssSI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I
HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I
NcoI NdeI NheI NspI PciI PflMI PfoI PpuMI
PsiI PvuII SacI SapI ScaI SexAI SfcI SmlI
SphI SspI StuI StyI TaqII TatI TstI XbaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AhdI ApaI AscI AsiSI
AvaI BbeI BciVI BclI BfrBI BglI BmgBI BplI
BsaHI BsiEI BsiWI BsmBI BspEI BsrBI BssHII BstAPI
BstZ17I Bsu36I ClaI DraIII EagI EciI EcoRV FalI
FseI FspI FspAI HaeII KasI KpnI MluI NaeI
NarI NgoMIV NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XcmI
XhoI XmaI ZraI