################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AvaI AviII BbeI BbrPI BciVI BfrI BglI BlpI BpmI Bpu1102I BsaAI BsaHI BseAI BsiEI BsiWI BsoBI BspEI BspHI BspLU11I BsrBI BsrFI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I KasI KspI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 272 GGTACC G^GTACC AccI 366 GTCGAC GT^[AC][GT]AC AccII 1171 CGCG CG^CG AciI 407 CCGC CCGC(-3/-1) Asp718I 272 GGTACC G^GTACC AvrII 4 CCTAGG C^CTAGG BanII 1289 GAGCTC G[GA]GC[CT]^C BbvCI 1435 GCTGAGG CCTCAGC(-5/-2) BcgI 1557 GCATGATATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2561 TGATCA T^GATCA BcnI 1635 CCGGG CC^[GC]GG BglII 2658 AGATCT A^GATCT BlnI 4 CCTAGG C^CTAGG BsaBI 2226 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 397 GGTCTC GGTCTC(1/5) BsaWI 2222 ACCGGA [AT]^CCGG[AT] BsmBI 263 CGTCTC CGTCTC(1/5) BsmI 1187 GCATTC GAATGC(1/-1) Bsp1407I 1821 TGTACA T^GTACA BspDI 1693 ATCGAT AT^CGAT BsrDI 1875 GCAATG GCAATG(2/0) BsrGI 1821 TGTACA T^GTACA BssSI 1810 CTCGTG CACGAG(-5/-1) BstBI 1540 TTCGAA TT^CGAA BstUI 1171 CGCG CG^CG ClaI 1693 ATCGAT AT^CGAT DraII 1256 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO109I 1256 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRV 1687 GATATC GAT^ATC FbaI 2561 TGATCA T^GATCA HpaI 1842 GTTAAC GTT^AAC KpnI 272 GGTACC GGTAC^C MaeII 2925 ACGT A^CGT MamI 2226 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 206 CAATTG C^AATTG MunI 206 CAATTG C^AATTG MvnI 1171 CGCG CG^CG NciI 1635 CCGGG CC^[GC]GG NcoI 908 CCATGG C^CATGG NspV 1540 TTCGAA TT^CGAA PflMI 3064 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1256 GGGACCC [GA]G^G[AT]CC[CT] SacI 1289 GAGCTC GAGCT^C SalI 366 GTCGAC G^TCGAC SapI 586 GAAGAGC GCTCTTC(1/4) SfuI 1540 TTCGAA TT^CGAA SphI 1046 GCATGC GCATG^C SspBI 1821 TGTACA T^GTACA SspI 1321 AATATT AAT^ATT StuI 580 AGGCCT AGG^CCT Van91I 3064 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### Asp700I 1109 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1423 GAAAAAATTC AspHI 1289 GAGCTC G[AT]GC[AT]^C 1401 GAGCAC BanI 272 GGTACC G^G[CT][GA]CC 904 GGTGCC BmyI 1289 GAGCTC G[AGT]GC[ACT]^C 1401 GAGCAC BseRI 1937 CTCCTC GAGGAG(10/8) 3077 GAGGAG BsiHKAI 1289 GAGCTC G[AT]GC[AT]^C 1401 GAGCAC Bsp1286I 1289 GAGCTC G[AGT]GC[ACT]^C 1401 GAGCAC DsaI 908 CCATGG C^C[GA][CT]GG 2581 CCACGG HapII 1635 CCGG C^CGG 2223 CCGG HincII 366 GTCGAC GT[CT]^[GA]AC 1842 GTTAAC HindII 366 GTCGAC GT[CT]^[GA]AC 1842 GTTAAC HpaII 1635 CCGG C^CGG 2223 CCGG MspI 1635 CCGG C^CGG 2223 CCGG SmlI 1388 CTCAAG C^T[CT][GA]AG 1940 CTCAAG XmnI 1109 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1423 GAAAAAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AcsI AfaI AluI AlwI AlwNI ApoI AvaII BalI BamHI BbsI BbvI BfaI BpuAI BsaJI BsgI BsiYI BslI BsmAI BsmFI BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII