(Circular) (Six-base) MAPSORT of: pFN21AE2544.seq Check: 7210 from: 1 to: 7936 pFN21AE2544 7936 bp With 182 enzymes: SgfI * September 30, 2014 11:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2154 2963 5041 2154 Size: 809 2078 5049 Fragments arranged by size: 5049 2078 809 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5746 278 Size: 53 83 186 936 4210 2468 Fragments arranged by size: 4210 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2372 5279 1937 Size: 435 2907 4594 Fragments arranged by size: 4594 2907 435 Acc65I G'GTAC_C Cuts at: 2277 5257 2277 Size: 2980 4956 AclI AA'CG_TT Cuts at: 1563 6063 6436 1563 Size: 4500 373 3063 Fragments arranged by size: 4500 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2610 2802 2990 3111 3504 3531 5999 7383 Size: 978 192 188 121 393 27 2468 1384 Cuts at: 7383 7651 1632 Size: 268 1917 Fragments arranged by size: 2468 1917 1384 978 393 268 192 188 121 27 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7739 AflIII A'CryG_T Cuts at: 1203 6835 7812 1203 Size: 5632 977 1327 Fragments arranged by size: 5632 1327 977 AgeI A'CCGG_T Cuts at: 6767 6767 Size: 7936 AhdI GACnn_n'nnGTC Cuts at: 6665 6665 Size: 7936 AleI CACnn'nnGTG Cuts at: 1166 3176 1166 Size: 2010 5926 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7936 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3518 3550 3518 Size: 32 7904 AlwNI CAG_nnn'CTG Cuts at: 2846 2978 4085 5679 7251 2846 Size: 132 1107 1594 1572 3531 Fragments arranged by size: 3531 1594 1572 1107 132 ApaLI G'TGCA_C Cuts at: 5993 7149 5993 Size: 1156 6780 ApoI r'AATT_y Cuts at: 1493 2630 2902 3023 3432 5245 5471 5623 7732 Size: 1137 272 121 409 1813 226 152 2109 Cuts at: 7732 1493 Size: 1697 Fragments arranged by size: 2109 1813 1697 1137 409 272 226 152 121 AseI AT'TA_AT Cuts at: 160 1032 6490 160 Size: 872 5458 1606 Fragments arranged by size: 5458 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7936 AvaI C'yCGr_G Cuts at: 1870 1943 5261 1870 Size: 73 3318 4545 Fragments arranged by size: 4545 3318 73 AvrII C'CTAG_G Cuts at: 2009 2009 Size: 7936 BamHI G'GATC_C Cuts at: 2307 3163 4591 5266 5633 2307 Size: 856 1428 675 367 4610 Fragments arranged by size: 4610 1428 856 675 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2277 2909 5257 6712 618 Size: 325 217 627 490 632 2348 1455 1842 Fragments arranged by size: 2348 1842 1455 632 627 490 325 217 BanII G_rGCy'C Cuts at: 1391 3298 5255 1391 Size: 1907 1957 4072 Fragments arranged by size: 4072 1957 1907 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3294 3762 4140 5185 928 Size: 971 1395 468 378 1045 3679 Fragments arranged by size: 3679 1395 1045 971 468 378 BbvCI CC'TCA_GC Cuts at: 3441 3441 Size: 7936 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3551 3585 6127 6161 1903 Size: 34 50 34 1530 34 2542 34 3678 Fragments arranged by size: 3678 2542 1530 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5828 7038 5828 Size: 1210 6726 BclI T'GATC_A Cuts at: 1548 4566 1548 Size: 3018 4918 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6547 136 Size: 107 122 71 631 605 4875 1525 Fragments arranged by size: 4875 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4663 7931 4663 Size: 3268 4668 BlpI GC'TnA_GC Cuts at: 5353 5353 Size: 7936 Bme1580I G_kGCm'C Cuts at: 1790 5997 7153 1790 Size: 4207 1156 2573 Fragments arranged by size: 4207 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7936 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3268 3784 3838 Size: 416 53 83 399 24 1851 516 54 Cuts at: 3838 5667 6625 442 Size: 1829 958 1753 Fragments arranged by size: 1851 1829 1753 958 516 416 399 83 54 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7936 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 6596 1239 Size: 5357 2579 Bpu10I CC'TnA_GC Cuts at: 3188 3441 5157 3188 Size: 253 1716 5967 Fragments arranged by size: 5967 1716 253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3378 3930 6061 6926 7224 3378 Size: 552 2131 865 298 4090 Fragments arranged by size: 4090 2131 865 552 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2408 6599 882 Size: 617 909 4191 2219 Fragments arranged by size: 4191 2219 909 617 BsaAI yAC'GTr Cuts at: 493 1204 5727 493 Size: 711 4523 2702 Fragments arranged by size: 4523 2702 711 BsaBI GATnn'nnATC Cuts at: 4235 7930 4235 Size: 3695 4241 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5743 6125 6760 275 Size: 53 83 186 936 4210 382 635 1451 Fragments arranged by size: 4210 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4227 6369 6767 7041 7188 7599 4227 Size: 2142 398 274 147 411 4564 Fragments arranged by size: 4564 2142 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4614 4644 1178 Size: 30 165 30 3211 30 4470 Fragments arranged by size: 4470 3211 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3933 5097 1186 Size: 2747 1164 4025 Fragments arranged by size: 4025 2747 1164 BseYI C'CCAG_C Cuts at: 2793 3856 4149 7139 7635 2793 Size: 1063 293 2990 496 3094 Fragments arranged by size: 3094 2990 1063 496 293 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3334 3877 5104 1163 Size: 573 1598 543 1227 3995 Fragments arranged by size: 3995 1598 1227 573 543 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5386 6147 7175 1540 Size: 275 186 3385 761 1028 2301 Fragments arranged by size: 3385 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3298 3410 5997 6082 7153 3298 Size: 112 2587 85 1071 4081 Fragments arranged by size: 4081 2587 1071 112 85 BsmI GAATG_Cn' Cuts at: 3192 5447 5540 3192 Size: 2255 93 5588 Fragments arranged by size: 5588 2255 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2274 2274 Size: 7936 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3298 3410 5255 5997 6082 7153 1391 Size: 399 1508 112 1845 742 85 1071 2174 Fragments arranged by size: 2174 1845 1508 1071 742 399 112 85 BspEI T'CCGG_A Cuts at: 7599 7599 Size: 7936 BspHI T'CATG_A Cuts at: 5825 7555 5825 Size: 1730 6206 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2154 2963 5041 5115 5291 844 Size: 1310 809 2078 74 176 3489 Fragments arranged by size: 3489 2078 1310 809 176 74 BsrBI CCG'CTC Cuts at: 1394 1823 5823 1394 Size: 429 4000 3507 Fragments arranged by size: 4000 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3887 6431 6605 65 Size: 1183 2639 2544 174 1396 Fragments arranged by size: 2639 2544 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6580 6767 1700 Size: 110 4770 187 2869 Fragments arranged by size: 4770 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3826 96 Size: 3730 4206 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7936 BssSI C'ACGA_G Cuts at: 3815 5996 7008 3815 Size: 2181 1012 4743 Fragments arranged by size: 4743 2181 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7936 BstBI TT'CG_AA Cuts at: 3546 6740 3546 Size: 3194 4742 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7936 BstXI CCAn_nnnn'nTGG Cuts at: 2132 2213 4161 2132 Size: 81 1948 5907 Fragments arranged by size: 5907 1948 81 BstYI r'GATC_y Cuts at: 1972 2307 3163 4591 4663 5266 5633 6018 6035 Size: 335 856 1428 72 603 367 385 17 Cuts at: 6035 7487 7573 7585 7931 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1428 856 603 385 367 346 335 86 72 17 12 BtgI C'CryG_G Cuts at: 513 1065 2913 4586 513 Size: 552 1848 1673 3863 Fragments arranged by size: 3863 1848 1673 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7197 Fragments arranged by size: 7197 683 56 BtsI GCAGTG_nn' Cuts at: 3760 3958 5452 6246 6266 3760 Size: 198 1494 794 20 5430 Fragments arranged by size: 5430 1494 794 198 20 ClaI AT'CG_AT Cuts at: 1554 3699 1554 Size: 2145 5791 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7901 DraI TTT'AAA Cuts at: 5240 5240 Size: 7936 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6943 809 Size: 507 5627 1802 Fragments arranged by size: 5627 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3205 3307 4087 Size: 54 1425 50 275 18 1375 102 780 Cuts at: 4087 5383 6272 7901 8 Size: 1296 889 1629 43 Fragments arranged by size: 1629 1425 1375 1296 889 780 275 102 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5383 1537 Size: 275 3571 4090 Fragments arranged by size: 4090 3571 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2586 2868 3507 3771 4160 4446 4818 5866 Size: 1233 282 639 264 389 286 372 1048 Cuts at: 5866 1353 Size: 3423 Fragments arranged by size: 3423 1233 1048 639 389 372 286 282 264 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6523 6897 7043 387 Size: 1064 138 4934 374 146 1280 Fragments arranged by size: 4934 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3296 3296 Size: 7936 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2610 2802 2990 3111 3504 3531 5999 Size: 393 978 192 188 121 393 27 2468 Cuts at: 5999 6596 7383 7651 1239 Size: 597 787 268 1524 Fragments arranged by size: 2468 1524 978 787 597 393 393 268 192 188 121 27 EcoO109I rG'GnC_Cy Cuts at: 3262 3262 Size: 7936 EcoRI G'AATT_C Cuts at: 5245 5623 5245 Size: 378 7558 EcoRV GAT'ATC Cuts at: 3694 3694 Size: 7936 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4157 4157 Size: 7936 FspI TGC'GCA Cuts at: 6442 6442 Size: 7936 HaeII r_GCGC'y Cuts at: 1678 7083 1678 Size: 5405 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2599 2631 2822 2854 3557 Size: 32 135 32 1027 32 191 32 703 Cuts at: 3557 3589 3681 3713 3770 3802 4614 4646 4948 Size: 32 92 32 57 32 812 32 302 Cuts at: 4948 4980 5196 5228 6577 6609 6651 6683 1373 Size: 32 216 32 1349 32 42 32 2626 Fragments arranged by size: 2626 1349 1027 812 703 302 216 191 135 92 57 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2373 3849 5280 5526 669 Size: 988 281 435 1476 1431 246 3079 Fragments arranged by size: 3079 1476 1431 988 435 281 246 HindIII A'AGCT_T Cuts at: 748 3060 3486 3528 4321 748 Size: 2312 426 42 793 4363 Fragments arranged by size: 4363 2312 793 426 42 HpaI GTT'AAC Cuts at: 3849 5526 3849 Size: 1677 6259 KpnI G_GTAC'C Cuts at: 2281 5261 2281 Size: 2980 4956 MfeI C'AATT_G Cuts at: 2211 5535 2211 Size: 3324 4612 MluI A'CGCG_T Cuts at: 7812 7812 Size: 7936 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3753 7050 7234 1305 Size: 2448 3297 184 2007 Fragments arranged by size: 3297 2448 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3207 3309 4089 10 Size: 54 1425 343 1375 102 780 3857 Fragments arranged by size: 3857 1425 1375 780 343 102 54 MslI CAynn'nnrTG Cuts at: 518 1166 3176 3365 4199 5894 6253 6412 518 Size: 648 2010 189 834 1695 359 159 2042 Fragments arranged by size: 2042 2010 1695 834 648 359 189 159 MspA1I CmG'CkG Cuts at: 1606 2846 3683 4330 5352 6029 7177 7422 1606 Size: 1240 837 647 1022 677 1148 245 2120 Fragments arranged by size: 2120 1240 1148 1022 837 677 647 245 NcoI C'CATG_G Cuts at: 513 1065 2913 513 Size: 552 1848 5536 Fragments arranged by size: 5536 1848 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7936 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7936 NotI GC'GGCC_GC Cuts at: 5383 5383 Size: 7936 NspI r_CATG'y Cuts at: 1132 1229 2947 3055 4776 5294 6839 1132 Size: 97 1718 108 1721 518 1545 2229 Fragments arranged by size: 2229 1721 1718 1545 518 108 97 PasI CC'CwG_GG Cuts at: 1348 5030 1348 Size: 3682 4254 PciI A'CATG_T Cuts at: 6835 6835 Size: 7936 PflMI CCAn_nnn'nTGG Cuts at: 1108 5075 1108 Size: 3967 3969 PfoI T'CCnGG_A Cuts at: 3639 3987 3639 Size: 348 7588 PmeI GTTT'AAAC Cuts at: 5240 5240 Size: 7936 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6005 6037 7518 7550 6005 Size: 32 1481 32 6391 Fragments arranged by size: 6391 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3262 3262 Size: 7936 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7936 PsiI TTA'TAA Cuts at: 5506 5506 Size: 7936 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4911 4943 4911 Size: 32 7904 PstI C_TGCA'G Cuts at: 830 3229 3477 3767 5112 5288 830 Size: 2399 248 290 1345 176 3478 Fragments arranged by size: 3478 2399 1345 290 248 176 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7936 PvuII CAG'CTG Cuts at: 2846 3683 4330 2846 Size: 837 647 6452 Fragments arranged by size: 6452 837 647 SacI G_AGCT'C Cuts at: 3298 3298 Size: 7936 SalI G'TCGA_C Cuts at: 1936 2371 5278 1936 Size: 435 2907 4594 Fragments arranged by size: 4594 2907 435 SanDI GG'GwC_CC Cuts at: 3262 3262 Size: 7936 SapI GCTCTTCn'nnn_ Cuts at: 2586 2586 Size: 7936 SbfI CC_TGCA'GG Cuts at: 5288 5288 Size: 7936 ScaI AGT'ACT Cuts at: 6184 7896 6184 Size: 1712 6224 SfcI C'TryA_G Cuts at: 826 1045 3225 3473 3654 3763 3867 4429 5108 Size: 219 2180 248 181 109 104 562 679 Cuts at: 5108 5284 6419 7100 7291 826 Size: 176 1135 681 191 1471 Fragments arranged by size: 2180 1471 1135 681 679 562 248 219 191 181 176 109 104 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7936 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7936 SmaI CCC'GGG Cuts at: 1872 5263 1872 Size: 3391 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3393 3945 6040 6941 7203 820 Size: 197 926 1450 552 2095 901 262 1553 Fragments arranged by size: 2095 1553 1450 926 901 552 262 197 SnaBI TAC'GTA Cuts at: 493 5727 493 Size: 5234 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7936 SphI G_CATG'C Cuts at: 1132 3055 5294 1132 Size: 1923 2239 3774 Fragments arranged by size: 3774 2239 1923 SspI AAT'ATT Cuts at: 5 52 3328 5860 5 Size: 47 3276 2532 2081 Fragments arranged by size: 3276 2532 2081 47 StuI AGG'CCT Cuts at: 1517 2587 1517 Size: 1070 6866 StyI C'CwwG_G Cuts at: 513 1065 2009 2282 2850 2913 2967 3837 5375 Size: 552 944 273 568 63 54 870 1538 Cuts at: 5375 513 Size: 3074 Fragments arranged by size: 3074 1538 944 870 568 552 273 63 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2660 3306 5963 5980 6133 6318 6763 Size: 1255 1080 646 2657 17 153 185 445 Cuts at: 6763 325 Size: 1498 Fragments arranged by size: 2657 1498 1255 1080 646 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2533 3255 3826 Size: 274 80 33 51 1443 556 722 571 Cuts at: 3826 6182 7894 96 Size: 2356 1712 138 Fragments arranged by size: 2356 1712 1443 722 571 556 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7896 7928 7896 Size: 32 7904 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7936 XbaI T'CTAG_A Cuts at: 5272 5272 Size: 7936 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7936 XmaI C'CCGG_G Cuts at: 1870 5261 1870 Size: 3391 4545 XmnI GAAnn'nnTTC Cuts at: 3118 3432 6065 3118 Size: 314 2633 4989 Fragments arranged by size: 4989 2633 314 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5744 276 Size: 53 83 186 936 4210 2468 Fragments arranged by size: 4210 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI