(Linear) (Six-base) MAPSORT of: KIEE2544.seq Check: 9220 from: 1 to: 3231 KIEE2544.orf With 182 enzymes: SgfI * September 30, 2014 10:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 149 958 3036 3231 Size: 149 809 2078 195 Fragments arranged by size: 2078 809 195 149 AccI GT'mk_AC Cuts at: 0 367 3231 Size: 367 2864 Acc65I G'GTAC_C Cuts at: 0 272 3231 Size: 272 2959 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 605 797 985 1106 1499 1526 3231 Size: 605 192 188 121 393 27 1705 Fragments arranged by size: 1705 605 393 192 188 121 27 AleI CACnn'nnGTG Cuts at: 0 1171 3231 Size: 1171 2060 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1513 1545 3231 Size: 1513 32 1686 Fragments arranged by size: 1686 1513 32 AlwNI CAG_nnn'CTG Cuts at: 0 841 973 2080 3231 Size: 841 132 1107 1151 Fragments arranged by size: 1151 1107 841 132 ApoI r'AATT_y Cuts at: 0 625 897 1018 1427 3231 Size: 625 272 121 409 1804 Fragments arranged by size: 1804 625 409 272 121 AvrII C'CTAG_G Cuts at: 0 4 3231 Size: 4 3227 BamHI G'GATC_C Cuts at: 0 302 1158 2586 3231 Size: 302 856 1428 645 Fragments arranged by size: 1428 856 645 302 BanI G'GyrC_C Cuts at: 0 272 904 3231 Size: 272 632 2327 Fragments arranged by size: 2327 632 272 BanII G_rGCy'C Cuts at: 0 1293 3231 Size: 1293 1938 BbsI GAAGACnn'nnnn_ Cuts at: 0 1289 1757 2135 3180 3231 Size: 1289 468 378 1045 51 Fragments arranged by size: 1289 1045 468 378 51 BbvCI CC'TCA_GC Cuts at: 0 1436 3231 Size: 1436 1795 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1546 1580 3231 Size: 1546 34 1651 Fragments arranged by size: 1651 1546 34 BclI T'GATC_A Cuts at: 0 2561 3231 Size: 2561 670 BglII A'GATC_T Cuts at: 0 2658 3231 Size: 2658 573 BmrI ACTGGGnnnn_n' Cuts at: 0 1263 1779 1833 3231 Size: 1263 516 54 1398 Fragments arranged by size: 1398 1263 516 54 Bpu10I CC'TnA_GC Cuts at: 0 1183 1436 3152 3231 Size: 1183 253 1716 79 Fragments arranged by size: 1716 1183 253 79 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1373 1925 3231 Size: 1373 552 1306 Fragments arranged by size: 1373 1306 552 BsaI GGTCTCn'nnnn_ Cuts at: 0 403 3231 Size: 403 2828 BsaBI GATnn'nnATC Cuts at: 0 2230 3231 Size: 2230 1001 BsaWI w'CCGG_w Cuts at: 0 2222 3231 Size: 2222 1009 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2609 2639 3231 Size: 2609 30 592 Fragments arranged by size: 2609 592 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1928 3092 3231 Size: 1928 1164 139 Fragments arranged by size: 1928 1164 139 BseYI C'CCAG_C Cuts at: 0 788 1851 2144 3231 Size: 788 1063 293 1087 Fragments arranged by size: 1087 1063 788 293 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1329 1872 3099 3231 Size: 1329 543 1227 132 Fragments arranged by size: 1329 1227 543 132 BsiHKAI G_wGCw'C Cuts at: 0 1293 1405 3231 Size: 1293 112 1826 Fragments arranged by size: 1826 1293 112 BsmI GAATG_Cn' Cuts at: 0 1187 3231 Size: 1187 2044 BsmBI CGTCTCn'nnnn_ Cuts at: 0 269 3231 Size: 269 2962 Bsp1286I G_dGCh'C Cuts at: 0 1293 1405 3231 Size: 1293 112 1826 Fragments arranged by size: 1826 1293 112 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 149 958 3036 3110 3231 Size: 149 809 2078 74 121 Fragments arranged by size: 2078 809 149 121 74 BsrDI GCAATG_nn' Cuts at: 0 1882 3231 Size: 1882 1349 BsrGI T'GTAC_A Cuts at: 0 1821 3231 Size: 1821 1410 BssSI C'ACGA_G Cuts at: 0 1810 3231 Size: 1810 1421 BstBI TT'CG_AA Cuts at: 0 1541 3231 Size: 1541 1690 BstXI CCAn_nnnn'nTGG Cuts at: 0 127 208 2156 3231 Size: 127 81 1948 1075 Fragments arranged by size: 1948 1075 127 81 BstYI r'GATC_y Cuts at: 0 302 1158 2586 2658 3231 Size: 302 856 1428 72 573 Fragments arranged by size: 1428 856 573 302 72 BtgI C'CryG_G Cuts at: 0 908 2581 3231 Size: 908 1673 650 Fragments arranged by size: 1673 908 650 BtsI GCAGTG_nn' Cuts at: 0 1755 1953 3231 Size: 1755 198 1278 Fragments arranged by size: 1755 1278 198 ClaI AT'CG_AT Cuts at: 0 1694 3231 Size: 1694 1537 EaeI y'GGCC_r Cuts at: 0 1200 1302 2082 3231 Size: 1200 102 780 1149 Fragments arranged by size: 1200 1149 780 102 EarI CTCTTCn'nnn_ Cuts at: 0 581 863 1502 1766 2155 2441 2813 3231 Size: 581 282 639 264 389 286 372 418 Fragments arranged by size: 639 581 418 389 372 286 282 264 EcoICRI GAG'CTC Cuts at: 0 1291 3231 Size: 1291 1940 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 605 797 985 1106 1499 1526 3231 Size: 605 192 188 121 393 27 1705 Fragments arranged by size: 1705 605 393 192 188 121 27 EcoO109I rG'GnC_Cy Cuts at: 0 1257 3231 Size: 1257 1974 EcoRV GAT'ATC Cuts at: 0 1689 3231 Size: 1689 1542 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2152 3231 Size: 2152 1079 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 594 626 817 849 1552 1584 1676 1708 Size: 594 32 191 32 703 32 92 32 Cuts at: 1708 1765 1797 2609 2641 2943 2975 3191 3223 Size: 57 32 812 32 302 32 216 32 Cuts at: 3223 3231 Size: 8 Fragments arranged by size: 812 703 594 302 216 191 92 57 32 32 32 32 32 32 32 32 8 HincII GTy'rAC Cuts at: 0 368 1844 3231 Size: 368 1476 1387 Fragments arranged by size: 1476 1387 368 HindIII A'AGCT_T Cuts at: 0 1055 1481 1523 2316 3231 Size: 1055 426 42 793 915 Fragments arranged by size: 1055 915 793 426 42 HpaI GTT'AAC Cuts at: 0 1844 3231 Size: 1844 1387 KpnI G_GTAC'C Cuts at: 0 276 3231 Size: 276 2955 MfeI C'AATT_G Cuts at: 0 206 3231 Size: 206 3025 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1748 3231 Size: 1748 1483 MscI TGG'CCA Cuts at: 0 1202 1304 2084 3231 Size: 1202 102 780 1147 Fragments arranged by size: 1202 1147 780 102 MslI CAynn'nnrTG Cuts at: 0 1171 1360 2194 3231 Size: 1171 189 834 1037 Fragments arranged by size: 1171 1037 834 189 MspA1I CmG'CkG Cuts at: 0 841 1678 2325 3231 Size: 841 837 647 906 Fragments arranged by size: 906 841 837 647 NcoI C'CATG_G Cuts at: 0 908 3231 Size: 908 2323 NspI r_CATG'y Cuts at: 0 942 1050 2771 3231 Size: 942 108 1721 460 Fragments arranged by size: 1721 942 460 108 PasI CC'CwG_GG Cuts at: 0 3025 3231 Size: 3025 206 PflMI CCAn_nnn'nTGG Cuts at: 0 3070 3231 Size: 3070 161 PfoI T'CCnGG_A Cuts at: 0 1634 1982 3231 Size: 1634 348 1249 Fragments arranged by size: 1634 1249 348 PpuMI rG'GwC_Cy Cuts at: 0 1257 3231 Size: 1257 1974 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2906 2938 3231 Size: 2906 32 293 Fragments arranged by size: 2906 293 32 PstI C_TGCA'G Cuts at: 0 1224 1472 1762 3107 3231 Size: 1224 248 290 1345 124 Fragments arranged by size: 1345 1224 290 248 124 PvuII CAG'CTG Cuts at: 0 841 1678 2325 3231 Size: 841 837 647 906 Fragments arranged by size: 906 841 837 647 SacI G_AGCT'C Cuts at: 0 1293 3231 Size: 1293 1938 SalI G'TCGA_C Cuts at: 0 366 3231 Size: 366 2865 SanDI GG'GwC_CC Cuts at: 0 1257 3231 Size: 1257 1974 SapI GCTCTTCn'nnn_ Cuts at: 0 581 3231 Size: 581 2650 SfcI C'TryA_G Cuts at: 0 1220 1468 1649 1758 1862 2424 3103 3231 Size: 1220 248 181 109 104 562 679 128 Fragments arranged by size: 1220 679 562 248 181 128 109 104 SmlI C'TyrA_G Cuts at: 0 1388 1940 3231 Size: 1388 552 1291 Fragments arranged by size: 1388 1291 552 SphI G_CATG'C Cuts at: 0 1050 3231 Size: 1050 2181 SspI AAT'ATT Cuts at: 0 1323 3231 Size: 1323 1908 StuI AGG'CCT Cuts at: 0 582 3231 Size: 582 2649 StyI C'CwwG_G Cuts at: 0 4 277 845 908 962 1832 3231 Size: 4 273 568 63 54 870 1399 Fragments arranged by size: 1399 870 568 273 63 54 4 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 655 1301 3231 Size: 655 646 1930 Fragments arranged by size: 1930 655 646 TatI w'GTAC_w Cuts at: 0 528 1250 1821 3231 Size: 528 722 571 1410 Fragments arranged by size: 1410 722 571 528 XmnI GAAnn'nnTTC Cuts at: 0 1113 1427 3231 Size: 1113 314 1804 Fragments arranged by size: 1804 1113 314 Enzymes that do cut: AarI AccI Acc65I AcuI AleI AloI AlwNI ApoI AvrII BamHI BanI BanII BbsI BbvCI BcgI BclI BglII BmrI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrGI BssSI BstBI BstXI BstYI BtgI BtsI ClaI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI Hin4I HincII HindIII HpaI KpnI MfeI MmeI MscI MslI MspA1I NcoI NspI PasI PflMI PfoI PpuMI PsrI PstI PvuII SacI SalI SanDI SapI SfcI SmlI SphI SspI StuI StyI TaqII TatI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AflIII AgeI AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvaI BaeI BbeI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI BpmI BsaAI BsaHI BsiEI BsiWI BspEI BspHI BsrBI BsrFI BssHII BstAPI BstEII BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI FseI FspI FspAI HaeII KasI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII SacII SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI