################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AciI 488 GCGG CCGC(-3/-1) AlwI 463 GGATC GGATC(4/5) Asp700I 12 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbvCI 477 GCTGAGG CCTCAGC(-5/-2) BciVI 849 GGATAC GTATCC(6/5) BclI 639 TGATCA T^GATCA BcnI 36 CCCGG CC^[GC]GG BpmI 130 CTGGAG CTGGAG(16/14) BsaI 623 GAGACC GGTCTC(1/5) BsmAI 623 GAGAC GTCTC(1/5) BsmI 544 GCATTC GAATGC(1/-1) Bsp1407I 687 TGTACA T^GTACA BspDI 76 ATCGAT AT^CGAT BspHI 642 TCATGA T^CATGA BsrGI 687 TGTACA T^GTACA BstAPI 310 GCAAATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 504 GGATG GGATG(2/0) BstXI 299 CCAGAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 137 GCGAGC GC[ACGT]^[ACGT]GC ClaI 76 ATCGAT AT^CGAT DdeI 478 CTGAG C^T[ACGT]AG DsaI 56 CCACGG C^C[GA][CT]GG EaeI 53 CGGCCA [CT]^GGCC[GA] EcoRV 238 GATATC GAT^ATC FbaI 639 TGATCA T^GATCA FokI 504 GGATG GGATG(9/13) HapII 37 CCGG C^CGG HpaII 37 CCGG C^CGG MaeIII 404 GTCAC ^GT[ACGT]AC MslI 406 CACAAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 486 CAGCGG C[AC]G^C[GT]G MspI 37 CCGG C^CGG NciI 36 CCCGG CC^[GC]GG PstI 243 CTGCAG CTGCA^G RcaI 642 TCATGA T^CATGA SfaNI 646 GATGC GCATC(5/9) SfcI 243 CTGCAG C^T[GA][CT]AG SspBI 687 TGTACA T^GTACA SspI 593 AATATT AAT^ATT TaqI 77 TCGA T^CGA Tsp45I 404 GTCAC ^GT[GC]AC TthHB8I 77 TCGA T^CGA XmnI 12 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AluI 278 AGCT AG^CT 399 AGCT BsrI 129 ACTGG ACTGG(1/-1) 627 CCAGT BstNI 445 CCTGG CC^[AT]GG 451 CCTGG Cfr13I 39 GGCCC G^G[ACGT]CC 448 GGCCC DpnII 464 GATC ^GATC 640 GATC EarI 115 GAAGAG CTCTTC(1/4) 499 GAAGAG EcoRII 445 CCTGG ^CC[AT]GG 451 CCTGG HphI 709 GGTGA GGTGA(8/7) 175 TCACC Ksp632I 115 GAAGAG CTCTTC(1/4) 499 GAAGAG MboI 464 GATC ^GATC 640 GATC MvaI 445 CCTGG CC^[AT]GG 451 CCTGG MwoI 310 GCAAATGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 435 GCGAAAATGGC NdeII 464 GATC ^GATC 640 GATC Sau3AI 464 GATC ^GATC 640 GATC Sau96I 39 GGCCC G^G[ACGT]CC 448 GGCCC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AcsI AfaI ApoI BbvI BsaJI BsiYI BslI BssKI Fnu4HI HaeIII HinfI ItaI MboII MnlI MseI NlaIII PleI RsaI ScrFI TfiI Tru9I TseI Tsp509I TspRI