Restriction map for
pFN21AE2297
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# NO CUT ENZYMES ( 78 enzymes) #
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AatII Acc65I AclI AgeI Aor51HI
AscI AseI AsnI Asp718I AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BlnI BsaAI BsaI BsiWI
BspDI BspHI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FbaI FspI
HincII HindII HindIII HpaI KasI
KpnI MfeI MluI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI
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# ONE CUT ENZYMES ( 24 enzymes) #
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AcsI 1744 GAATTT [GA]^AATT[CT]
AflIII 1813 ACATGT A^C[GA][CT]GT
ApoI 1744 GAATTT [GA]^AATT[CT]
BamHI 4354 GGATCC G^GATCC
BsaBI 2355 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2147 CGGTCG CG[GA][CT]^CG
BsmBI 3830 GAGACG CGTCTC(1/5)
BspLU11I 1813 ACATGT A^CATGT
DraIII 3721 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
FseI 531 GGCCGGCC GGCCGG^CC
KspI 2250 CCGCGG CCGC^GG
MaeII 3187 ACGT A^CGT
MamI 2355 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 3009 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 442 CTCGAG C^TCGAG
SacII 2250 CCGCGG CCGC^GG
ScaI 3905 AGTACT AGT^ACT
SexAI 2720 ACCAGGT A^CC[AT]GGT
SfiI 2700 GGCCCCTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1436 CCCGGG CCC^GGG
Tsp509I 1745 AATT ^AATT
XhoI 442 CTCGAG C^TCGAG
XmaCI 1436 CCCGGG C^CCGGG
XmaI 1436 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccI 121 GTAGAC GT^[AC][GT]AC
3796 GTAGAC
AcyI 3044 GACGCC G[GA]^CG[CT]C
3637 GACGCC
AflII 2002 CTTAAG C^TTAAG
4627 CTTAAG
AhdI 102 GACTGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1469 GACAGATTGTC
Alw44I 1222 GTGCAC G^TGCAC
2853 GTGCAC
ApaI 236 GGGCCC GGGCC^C
4174 GGGCCC
ApaLI 1222 GTGCAC G^TGCAC
2853 GTGCAC
Asp700I 2649 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3264 GAACCACTTC
AspEI 102 GACTGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1469 GACAGATTGTC
AspI 86 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3884 GACCCAGTC
BbvCI 564 CCTCAGC CCTCAGC(-5/-2)
4432 GCTGAGG
BfrI 2002 CTTAAG C^TTAAG
4627 CTTAAG
BglII 584 AGATCT A^GATCT
778 AGATCT
BsaHI 3044 GACGCC G[GA]^CG[CT]C
3637 GACGCC
Bsp1407I 2687 TGTACA T^GTACA
2939 TGTACA
BsrGI 2687 TGTACA T^GTACA
2939 TGTACA
BstEII 2724 GGTGACC G^GT[ACGT]ACC
4923 GGTCACC
BstPI 2724 GGTGACC G^GT[ACGT]ACC
4923 GGTCACC
DrdI 3852 GACACCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4915 GACTCGGCGGTC
Eam1105I 102 GACTGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1469 GACAGATTGTC
EcoO65I 2724 GGTGACC G^GT[ACGT]ACC
4923 GGTCACC
Hin1I 3044 GACGCC G[GA]^CG[CT]C
3637 GACGCC
MseI 2003 TTAA T^TAA
4628 TTAA
NcoI 21 CCATGG C^CATGG
971 CCATGG
PflFI 86 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3884 GACCCAGTC
PshAI 2749 GACTGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3402 GACCTCCGTC
PstI 361 CTGCAG CTGCA^G
1177 CTGCAG
SapI 829 GAAGAGC GCTCTTC(1/4)
1717 GAAGAGC
SspBI 2687 TGTACA T^GTACA
2939 TGTACA
Tru9I 2003 TTAA T^TAA
4628 TTAA
Tth111I 86 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3884 GACCCAGTC
XmnI 2649 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3264 GAACCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII