(Circular) (Six-base) MAPSORT of: pFN21AE2297.seq Check: 9148 from: 1 to: 9685 pFN21AE2297 9685 bp With 182 enzymes: SgfI * November 4, 2010 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3920 4057 4088 3920 Size: 137 31 9517 Fragments arranged by size: 9517 137 31 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7495 278 Size: 53 83 186 936 5959 2468 Fragments arranged by size: 5959 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2127 5802 7028 1937 Size: 190 3675 1226 4594 Fragments arranged by size: 4594 3675 1226 190 Acc65I G'GTAC_C Cuts at: 7006 7006 Size: 9685 AclI AA'CG_TT Cuts at: 1563 7812 8185 1563 Size: 6249 373 3063 Fragments arranged by size: 6249 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2853 2922 3104 3237 4790 5234 5260 5261 Size: 1221 69 182 133 1553 444 26 1 Cuts at: 5261 6400 6407 7748 9132 9400 1632 Size: 1139 7 1341 1384 268 1917 Fragments arranged by size: 1917 1553 1384 1341 1221 1139 444 268 182 133 69 26 7 1 AflII C'TTAA_G Cuts at: 820 1017 4007 6632 820 Size: 197 2990 2625 3873 Fragments arranged by size: 3873 2990 2625 197 AflIII A'CryG_T Cuts at: 1203 3818 8584 9561 1203 Size: 2615 4766 977 1327 Fragments arranged by size: 4766 2615 1327 977 AgeI A'CCGG_T Cuts at: 8516 8516 Size: 9685 AhdI GACnn_n'nnGTC Cuts at: 2112 3479 8414 2112 Size: 1367 4935 3383 Fragments arranged by size: 4935 3383 1367 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9685 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9685 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6519 6551 6519 Size: 32 9653 AlwNI CAG_nnn'CTG Cuts at: 2327 3833 4669 5500 6333 6904 6978 7428 9000 Size: 1506 836 831 833 571 74 450 1572 Cuts at: 9000 2327 Size: 3012 Fragments arranged by size: 3012 1572 1506 836 833 831 571 450 74 ApaI G_GGCC'C Cuts at: 2245 6183 2245 Size: 3938 5747 ApaLI G'TGCA_C Cuts at: 3227 4858 7742 8898 3227 Size: 1631 2884 1156 4014 Fragments arranged by size: 4014 2884 1631 1156 ApoI r'AATT_y Cuts at: 1493 3749 6994 7220 7372 9481 1493 Size: 2256 3245 226 152 2109 1697 Fragments arranged by size: 3245 2256 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 8239 160 Size: 872 7207 1606 Fragments arranged by size: 7207 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9685 AvaI C'yCGr_G Cuts at: 1870 1943 2447 3441 3803 4913 5057 7010 1870 Size: 73 504 994 362 1110 144 1953 4545 Fragments arranged by size: 4545 1953 1110 994 504 362 144 73 BamHI G'GATC_C Cuts at: 6359 7015 7382 6359 Size: 656 367 8662 Fragments arranged by size: 8662 656 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2059 2581 3079 4326 4529 Size: 325 217 627 272 522 498 1247 203 Cuts at: 4529 5510 7006 8461 618 Size: 981 1496 1455 1842 Fragments arranged by size: 1842 1496 1455 1247 981 627 522 498 325 272 217 203 BanII G_rGCy'C Cuts at: 1391 2085 2245 2809 2945 3153 3560 3729 3842 Size: 694 160 564 136 208 407 169 113 Cuts at: 3842 4063 4253 4428 5588 5765 6143 6183 6385 Size: 221 190 175 1160 177 378 40 202 Cuts at: 6385 6447 6861 7004 1391 Size: 62 414 143 4072 Fragments arranged by size: 4072 1160 694 564 414 407 378 221 208 202 190 177 175 169 160 143 136 113 62 40 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2358 3873 4893 5476 6667 928 Size: 971 459 1515 1020 583 1191 3946 Fragments arranged by size: 3946 1515 1191 1020 971 583 459 BbvCI CC'TCA_GC Cuts at: 2570 6438 2570 Size: 3868 5817 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7876 7910 1903 Size: 34 5939 34 3678 Fragments arranged by size: 5939 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7577 8787 7577 Size: 1210 8475 BclI T'GATC_A Cuts at: 1548 1548 Size: 9685 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2210 2681 4712 Size: 107 122 71 631 605 538 471 2031 Cuts at: 4712 5102 6311 8296 136 Size: 390 1209 1985 1525 Fragments arranged by size: 2031 1985 1525 1209 631 605 538 471 390 122 107 71 BglII A'GATC_T Cuts at: 2589 2783 9680 2589 Size: 194 6897 2594 Fragments arranged by size: 6897 2594 194 BlpI GC'TnA_GC Cuts at: 2045 5141 5820 6855 7102 2045 Size: 3096 679 1035 247 4628 Fragments arranged by size: 4628 3096 1035 679 247 Bme1580I G_kGCm'C Cuts at: 1790 2245 2586 2629 2915 3231 3570 3792 4822 Size: 455 341 43 286 316 339 222 1030 Cuts at: 4822 4862 5523 5832 6183 6604 6760 6766 6845 Size: 40 661 309 351 421 156 6 79 Cuts at: 6845 6915 7746 8902 1790 Size: 70 831 1156 2573 Fragments arranged by size: 2573 1156 1030 831 661 455 421 351 341 339 316 309 286 222 156 79 70 43 40 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9685 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2088 2118 2152 Size: 416 53 83 399 24 671 30 34 Cuts at: 2152 2785 3396 5886 7416 8374 442 Size: 633 611 2490 1530 958 1753 Fragments arranged by size: 2490 1753 1530 958 671 633 611 416 399 83 53 34 30 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9685 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2353 3100 3147 4430 5335 6137 2353 Size: 747 47 1283 905 802 5901 Fragments arranged by size: 5901 1283 905 802 747 47 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2477 2859 2910 3008 3378 3598 4319 4576 Size: 1238 382 51 98 370 220 721 257 Cuts at: 4576 5195 6132 6443 8345 1239 Size: 619 937 311 1902 2579 Fragments arranged by size: 2579 1902 1238 937 721 619 382 370 311 257 220 98 51 Bpu10I CC'TnA_GC Cuts at: 2246 2570 2630 3494 5582 6438 2246 Size: 324 60 864 2088 856 5493 Fragments arranged by size: 5493 2088 864 856 324 60 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5237 7810 8675 8973 5237 Size: 2573 865 298 5949 Fragments arranged by size: 5949 2573 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 8348 882 Size: 617 6849 2219 Fragments arranged by size: 6849 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 7476 493 Size: 711 6272 2702 Fragments arranged by size: 6272 2702 711 BsaBI GATnn'nnATC Cuts at: 4364 9679 4364 Size: 5315 4370 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5050 5643 7492 7874 Size: 53 83 186 936 3517 593 1849 382 Cuts at: 7874 8509 275 Size: 635 1451 Fragments arranged by size: 3517 1849 1451 936 635 593 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4209 4440 4488 4650 5149 8118 8516 8790 8937 Size: 231 48 162 499 2969 398 274 147 Cuts at: 8937 9348 4209 Size: 411 4546 Fragments arranged by size: 4546 2969 499 411 398 274 231 162 147 48 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2981 3011 3221 3251 3351 Size: 30 165 30 1578 30 210 30 100 Cuts at: 3351 3381 4169 4199 5264 5294 6389 6419 6497 Size: 30 788 30 1065 30 1095 30 78 Cuts at: 6497 6500 6527 6530 1178 Size: 3 27 3 4333 Fragments arranged by size: 4333 1578 1095 1065 788 210 165 100 78 30 30 30 30 30 30 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2510 2558 2689 3941 4193 4316 4994 5649 Size: 1324 48 131 1252 252 123 678 655 Cuts at: 5649 6302 6410 6440 6455 6485 6509 6518 6521 Size: 653 108 30 15 30 24 9 3 Cuts at: 6521 6788 1186 Size: 267 4083 Fragments arranged by size: 4083 1324 1252 678 655 653 267 252 131 123 108 48 30 30 24 15 9 3 BseYI C'CCAG_C Cuts at: 2101 2161 2294 2545 3502 3656 3826 5229 5326 Size: 60 133 251 957 154 170 1403 97 Cuts at: 5326 5399 5621 5964 6176 6182 6208 6449 6571 Size: 73 222 343 212 6 26 241 122 Cuts at: 6571 6787 6861 8888 9384 2101 Size: 216 74 2027 496 2402 Fragments arranged by size: 2402 2027 1403 957 496 343 251 241 222 216 212 170 154 133 122 97 74 73 60 26 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3539 4855 5555 6599 1163 Size: 573 1803 1316 700 1044 4249 Fragments arranged by size: 4249 1803 1316 1044 700 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4155 7135 7896 8924 1540 Size: 275 186 2154 2980 761 1028 2301 Fragments arranged by size: 2980 2301 2154 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2841 2945 3231 3729 3903 4253 4428 4650 4862 Size: 104 286 498 174 350 175 222 212 Cuts at: 4862 5025 5147 5765 7746 7831 8902 2841 Size: 163 122 618 1981 85 1071 3624 Fragments arranged by size: 3624 1981 1071 618 498 350 286 222 212 175 174 163 122 104 85 BsmI GAATG_Cn' Cuts at: 2438 4389 4996 7196 7289 2438 Size: 1951 607 2200 93 4834 Fragments arranged by size: 4834 2200 1951 607 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5829 5829 Size: 9685 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2245 2586 2629 2809 2841 2915 Size: 399 295 160 341 43 180 32 74 Cuts at: 2915 2945 3153 3231 3560 3570 3729 3792 3842 Size: 30 208 78 329 10 159 63 50 Cuts at: 3842 3903 4063 4253 4428 4650 4822 4862 5025 Size: 61 160 190 175 222 172 40 163 Cuts at: 5025 5147 5523 5588 5765 5832 6143 6183 6385 Size: 122 376 65 177 67 311 40 202 Cuts at: 6385 6447 6604 6760 6766 6845 6861 6915 7004 Size: 62 157 156 6 79 16 54 89 Cuts at: 7004 7746 7831 8902 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 376 341 329 311 295 222 208 202 190 180 177 175 172 163 160 160 159 157 156 122 89 85 79 78 74 67 65 63 62 61 54 50 43 40 40 32 30 16 10 6 BspEI T'CCGG_A Cuts at: 4209 4440 4650 5149 9348 4209 Size: 231 210 499 4199 4546 Fragments arranged by size: 4546 4199 499 231 210 BspHI T'CATG_A Cuts at: 7574 9304 7574 Size: 1730 7955 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3920 4057 4088 4502 7040 844 Size: 3076 137 31 414 2538 3489 Fragments arranged by size: 3489 3076 2538 414 137 31 BsrBI CCG'CTC Cuts at: 1394 1823 2500 4183 6461 7572 1394 Size: 429 677 1683 2278 1111 3507 Fragments arranged by size: 3507 2278 1683 1111 677 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2634 2670 4266 8180 8354 65 Size: 1183 1386 36 1596 3914 174 1396 Fragments arranged by size: 3914 1596 1396 1386 1183 174 36 BsrFI r'CCGG_y Cuts at: 1700 1810 2062 2537 3532 5337 6433 8329 8516 Size: 110 252 475 995 1805 1096 1896 187 Cuts at: 8516 1700 Size: 2869 Fragments arranged by size: 2869 1896 1805 1096 995 475 252 187 110 BsrGI T'GTAC_A Cuts at: 96 4692 4944 96 Size: 4596 252 4837 Fragments arranged by size: 4837 4596 252 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9685 BssSI C'ACGA_G Cuts at: 7745 8757 7745 Size: 1012 8673 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9685 BstBI TT'CG_AA Cuts at: 8489 8489 Size: 9685 BstEII G'GTnAC_C Cuts at: 1184 4729 6928 1184 Size: 3545 2199 3941 Fragments arranged by size: 3941 3545 2199 BstXI CCAn_nnnn'nTGG Cuts at: 2101 6037 6322 2101 Size: 3936 285 5464 Fragments arranged by size: 5464 3936 285 BstYI r'GATC_y Cuts at: 1972 2589 2783 4139 5780 6359 7015 7382 7767 Size: 617 194 1356 1641 579 656 367 385 Cuts at: 7767 7784 9236 9322 9334 9680 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1641 1452 1356 656 617 579 385 367 346 194 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2053 2328 2754 3042 4710 2053 Size: 275 426 288 1668 7028 Fragments arranged by size: 7028 1668 426 288 275 BtgI C'CryG_G Cuts at: 513 1065 2004 2026 2976 3302 3366 4149 4255 Size: 552 939 22 950 326 64 783 106 Cuts at: 4255 4863 6134 6200 6564 6577 6709 513 Size: 608 1271 66 364 13 132 3489 Fragments arranged by size: 3489 1271 950 939 783 608 552 364 326 132 106 66 64 22 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2436 487 Size: 683 56 1210 7736 Fragments arranged by size: 7736 1210 683 56 BtsI GCAGTG_nn' Cuts at: 2363 4342 5539 7201 7995 8015 2363 Size: 1979 1197 1662 794 20 4033 Fragments arranged by size: 4033 1979 1662 1197 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9685 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4507 4542 529 Size: 35 3943 35 5672 Fragments arranged by size: 5672 3943 35 35 DraI TTT'AAA Cuts at: 6989 6989 Size: 9685 DraIII CAC_nnn'GTG Cuts at: 5731 5731 Size: 9685 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5863 6926 8692 809 Size: 507 4547 1063 1766 1802 Fragments arranged by size: 4547 1802 1766 1063 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2217 2318 4671 Size: 54 1425 50 275 18 387 101 2353 Cuts at: 4671 4953 6061 6561 7132 8021 9650 8 Size: 282 1108 500 571 889 1629 43 Fragments arranged by size: 2353 1629 1425 1108 889 571 500 387 282 275 101 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7132 1537 Size: 275 5320 4090 Fragments arranged by size: 5320 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2829 3091 3218 3464 3702 3717 4649 6495 Size: 1476 262 127 246 238 15 932 1846 Cuts at: 6495 7615 1353 Size: 1120 3423 Fragments arranged by size: 3423 1846 1476 1120 932 262 246 238 127 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4601 6764 8272 8646 8792 387 Size: 1064 138 3012 2163 1508 374 146 1280 Fragments arranged by size: 3012 2163 1508 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2943 3727 4251 4426 5763 2943 Size: 784 524 175 1337 6865 Fragments arranged by size: 6865 1337 784 524 175 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2477 2853 2859 2910 2922 3008 3104 Size: 393 845 376 6 51 12 86 96 Cuts at: 3104 3237 3378 3598 4319 4576 4790 5195 5234 Size: 133 141 220 721 257 214 405 39 Cuts at: 5234 5260 5261 6132 6400 6407 6443 7748 8345 Size: 26 1 871 268 7 36 1305 597 Cuts at: 8345 9132 9400 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1305 871 845 787 721 597 405 393 376 268 268 257 220 214 141 133 96 86 51 39 36 26 12 7 6 1 EcoNI CCTnn'n_nnAGG Cuts at: 2753 3221 4418 4880 6425 2753 Size: 468 1197 462 1545 6013 Fragments arranged by size: 6013 1545 1197 468 462 EcoO109I rG'GnC_Cy Cuts at: 2114 2241 2242 2312 2324 2516 2605 2683 3046 Size: 127 1 70 12 192 89 78 363 Cuts at: 3046 3362 3438 3498 3651 3799 4666 5170 5504 Size: 316 76 60 153 148 867 504 334 Cuts at: 5504 5687 5713 5740 5888 6330 6409 2114 Size: 183 26 27 148 442 79 5390 Fragments arranged by size: 5390 867 504 442 363 334 316 192 183 153 148 148 127 89 79 78 76 70 60 27 26 12 1 EcoRI G'AATT_C Cuts at: 6994 7372 6994 Size: 378 9307 FseI GG_CCGG'CC Cuts at: 2541 2541 Size: 9685 FspI TGC'GCA Cuts at: 8191 8191 Size: 9685 HaeII r_GCGC'y Cuts at: 1678 2052 3009 6209 8832 1678 Size: 374 957 3200 2623 2531 Fragments arranged by size: 3200 2623 2531 957 374 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2098 2130 2747 2779 2828 Size: 32 135 32 526 32 617 32 49 Cuts at: 2828 2860 3465 3497 4461 4493 6212 6244 6500 Size: 32 605 32 964 32 1719 32 256 Cuts at: 6500 6532 8326 8358 8400 8432 1373 Size: 32 1794 32 42 32 2626 Fragments arranged by size: 2626 1794 1719 964 617 605 526 256 135 49 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 7029 7275 669 Size: 988 281 5091 246 3079 Fragments arranged by size: 5091 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9685 HpaI GTT'AAC Cuts at: 7275 7275 Size: 9685 KpnI G_GTAC'C Cuts at: 7010 7010 Size: 9685 MfeI C'AATT_G Cuts at: 7284 7284 Size: 9685 MluI A'CGCG_T Cuts at: 9561 9561 Size: 9685 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3496 5071 5175 5370 5790 6498 8799 8983 Size: 2191 1575 104 195 420 708 2301 184 Cuts at: 8983 1305 Size: 2007 Fragments arranged by size: 2301 2191 2007 1575 708 420 195 184 104 MscI TGG'CCA Cuts at: 10 64 1489 1832 4673 4955 6063 10 Size: 54 1425 343 2841 282 1108 3632 Fragments arranged by size: 3632 2841 1425 1108 343 282 54 MslI CAynn'nnrTG Cuts at: 518 1166 5018 7643 8002 8161 518 Size: 648 3852 2625 359 159 2042 Fragments arranged by size: 3852 2625 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2087 3356 4257 4927 5007 5083 5089 5295 Size: 481 1269 901 670 80 76 6 206 Cuts at: 5295 5809 5964 6033 6322 6648 6745 6978 7101 Size: 514 155 69 289 326 97 233 123 Cuts at: 7101 7778 8926 9171 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1269 1148 901 677 670 514 481 326 289 245 233 206 155 123 97 80 76 69 6 NaeI GCC'GGC Cuts at: 2539 5339 6435 2539 Size: 2800 1096 5789 Fragments arranged by size: 5789 2800 1096 NcoI C'CATG_G Cuts at: 513 1065 2004 2026 2976 513 Size: 552 939 22 950 7222 Fragments arranged by size: 7222 950 939 552 22 NdeI CA'TA_TG Cuts at: 387 387 Size: 9685 NgoMIV G'CCGG_C Cuts at: 2537 5337 6433 2537 Size: 2800 1096 5789 Fragments arranged by size: 5789 2800 1096 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9685 NotI GC'GGCC_GC Cuts at: 7132 7132 Size: 9685 NspI r_CATG'y Cuts at: 1132 1229 3822 4564 4934 5932 7043 8588 1132 Size: 97 2593 742 370 998 1111 1545 2229 Fragments arranged by size: 2593 2229 1545 1111 998 742 370 97 PasI CC'CwG_GG Cuts at: 1348 2513 3389 6355 1348 Size: 1165 876 2966 4678 Fragments arranged by size: 4678 2966 1165 876 PciI A'CATG_T Cuts at: 3818 8584 3818 Size: 4766 4919 PflMI CCAn_nnn'nTGG Cuts at: 1108 2197 4669 6715 1108 Size: 1089 2472 2046 4078 Fragments arranged by size: 4078 2472 2046 1089 PfoI T'CCnGG_A Cuts at: 3483 4164 4553 4662 6537 3483 Size: 681 389 109 1875 6631 Fragments arranged by size: 6631 1875 681 389 109 PmeI GTTT'AAAC Cuts at: 6989 6989 Size: 9685 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6149 6181 7754 7786 9267 9299 6149 Size: 32 1573 32 1481 32 6535 Fragments arranged by size: 6535 1573 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2114 2312 3362 3498 3651 3799 4666 5504 5740 Size: 198 1050 136 153 148 867 838 236 Cuts at: 5740 5888 6409 2114 Size: 148 521 5390 Fragments arranged by size: 5390 1050 867 838 521 236 198 153 148 148 136 PshAI GACnn'nnGTC Cuts at: 1613 4758 5411 1613 Size: 3145 653 5887 Fragments arranged by size: 5887 3145 653 PsiI TTA'TAA Cuts at: 7255 7255 Size: 9685 PspOMI G'GGCC_C Cuts at: 2241 6179 2241 Size: 3938 5747 PspXI vC'TCGA_Gb Cuts at: 2447 2447 Size: 9685 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5354 5386 5354 Size: 32 9653 PstI C_TGCA'G Cuts at: 830 2370 3186 7037 830 Size: 1540 816 3851 3478 Fragments arranged by size: 3851 3478 1540 816 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9685 PvuII CAG'CTG Cuts at: 3356 4927 5089 5964 6033 6322 6745 3356 Size: 1571 162 875 69 289 423 6296 Fragments arranged by size: 6296 1571 875 423 289 162 69 SacI G_AGCT'C Cuts at: 2945 3729 4253 4428 5765 2945 Size: 784 524 175 1337 6865 Fragments arranged by size: 6865 1337 784 524 175 SacII CC_GC'GG Cuts at: 4258 4258 Size: 9685 SalI G'TCGA_C Cuts at: 1936 7027 1936 Size: 5091 4594 SanDI GG'GwC_CC Cuts at: 2312 5740 6409 2312 Size: 3428 669 5588 Fragments arranged by size: 5588 3428 669 SapI GCTCTTCn'nnn_ Cuts at: 2829 3717 2829 Size: 888 8797 SbfI CC_TGCA'GG Cuts at: 7037 7037 Size: 9685 ScaI AGT'ACT Cuts at: 5912 7933 9645 5912 Size: 2021 1712 5952 Fragments arranged by size: 5952 2021 1712 SexAI A'CCwGG_T Cuts at: 4725 4725 Size: 9685 SfcI C'TryA_G Cuts at: 826 1045 2366 3182 6970 7033 8168 8849 9040 Size: 219 1321 816 3788 63 1135 681 191 Cuts at: 9040 826 Size: 1471 Fragments arranged by size: 3788 1471 1321 1135 816 681 219 191 63 SfiI GGCCn_nnn'nGGCC Cuts at: 4712 4712 Size: 9685 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9685 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9685 SmaI CCC'GGG Cuts at: 1872 3443 7012 1872 Size: 1571 3569 4545 Fragments arranged by size: 4545 3569 1571 SmlI C'TyrA_G Cuts at: 820 1017 1943 2447 4007 5252 6632 7789 8690 Size: 197 926 504 1560 1245 1380 1157 901 Cuts at: 8690 8952 820 Size: 262 1553 Fragments arranged by size: 1560 1553 1380 1245 1157 926 901 504 262 197 SnaBI TAC'GTA Cuts at: 493 7476 493 Size: 6983 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9685 SphI G_CATG'C Cuts at: 1132 7043 1132 Size: 5911 3774 SspI AAT'ATT Cuts at: 5 52 7609 5 Size: 47 7557 2081 Fragments arranged by size: 7557 2081 47 StuI AGG'CCT Cuts at: 1517 2759 4715 5205 5238 1517 Size: 1242 1956 490 33 5964 Fragments arranged by size: 5964 1956 1242 490 33 StyI C'CwwG_G Cuts at: 513 1065 2004 2026 2117 2640 2976 3063 3324 Size: 552 939 22 91 523 336 87 261 Cuts at: 3324 3954 4504 4881 5691 5709 6171 7124 513 Size: 630 550 377 810 18 462 953 3074 Fragments arranged by size: 3074 953 939 810 630 552 550 523 462 377 336 261 91 87 22 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2422 3173 3337 3964 3991 4039 4267 Size: 1255 842 751 164 627 27 48 228 Cuts at: 4267 4845 5945 7712 7729 7882 8067 8512 325 Size: 578 1100 1767 17 153 185 445 1498 Fragments arranged by size: 1767 1498 1255 1100 842 751 627 578 445 228 185 164 153 48 27 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3115 4563 4692 Size: 274 80 33 51 1443 1138 1448 129 Cuts at: 4692 4944 5289 5370 5910 7931 9643 96 Size: 252 345 81 540 2021 1712 138 Fragments arranged by size: 2021 1712 1448 1443 1138 540 345 274 252 138 129 81 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3221 3253 3351 3383 5264 5296 5717 5749 5935 Size: 32 98 32 1881 32 421 32 186 Cuts at: 5935 5967 6389 6421 6497 6529 9645 9677 3221 Size: 32 422 32 76 32 3116 32 3229 Fragments arranged by size: 3229 3116 1881 422 421 186 98 76 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2094 5892 1316 Size: 778 3798 5109 Fragments arranged by size: 5109 3798 778 XbaI T'CTAG_A Cuts at: 7021 7021 Size: 9685 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2983 3070 4036 6056 6712 2983 Size: 87 966 2020 656 5956 Fragments arranged by size: 5956 2020 966 656 87 XhoI C'TCGA_G Cuts at: 1943 2447 1943 Size: 504 9181 XmaI C'CCGG_G Cuts at: 1870 3441 7010 1870 Size: 1571 3569 4545 Fragments arranged by size: 4545 3569 1571 XmnI GAAnn'nnTTC Cuts at: 4658 5273 7814 4658 Size: 615 2541 6529 Fragments arranged by size: 6529 2541 615 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7493 276 Size: 53 83 186 936 5959 2468 Fragments arranged by size: 5959 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI RsrII SfoI SrfI SwaI