(Linear) (Six-base) MAPSORT of: KIEE2297.seq Check: 9431 from: 1 to: 4980 KIEE2297.orf With 182 enzymes: SgfI * November 2, 2010 17:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1915 2052 2083 4980 Size: 1915 137 31 2897 Fragments arranged by size: 2897 1915 137 31 AccI GT'mk_AC Cuts at: 0 122 3797 4980 Size: 122 3675 1183 Fragments arranged by size: 3675 1183 122 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 848 917 1099 1232 2785 3229 3255 3256 Size: 848 69 182 133 1553 444 26 1 Cuts at: 3256 4395 4402 4980 Size: 1139 7 578 Fragments arranged by size: 1553 1139 848 578 444 182 133 69 26 7 1 AflII C'TTAA_G Cuts at: 0 2002 4627 4980 Size: 2002 2625 353 Fragments arranged by size: 2625 2002 353 AflIII A'CryG_T Cuts at: 0 1813 4980 Size: 1813 3167 AhdI GACnn_n'nnGTC Cuts at: 0 107 1474 4980 Size: 107 1367 3506 Fragments arranged by size: 3506 1367 107 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 4514 4546 4980 Size: 4514 32 434 Fragments arranged by size: 4514 434 32 AlwNI CAG_nnn'CTG Cuts at: 0 322 1828 2664 3495 4328 4899 4973 4980 Size: 322 1506 836 831 833 571 74 7 Fragments arranged by size: 1506 836 833 831 571 322 74 7 ApaI G_GGCC'C Cuts at: 0 240 4178 4980 Size: 240 3938 802 Fragments arranged by size: 3938 802 240 ApaLI G'TGCA_C Cuts at: 0 1222 2853 4980 Size: 1222 1631 2127 Fragments arranged by size: 2127 1631 1222 ApoI r'AATT_y Cuts at: 0 1744 4980 Size: 1744 3236 AvaI C'yCGr_G Cuts at: 0 442 1436 1798 2908 3052 4980 Size: 442 994 362 1110 144 1928 Fragments arranged by size: 1928 1110 994 442 362 144 BamHI G'GATC_C Cuts at: 0 4354 4980 Size: 4354 626 BanI G'GyrC_C Cuts at: 0 54 576 1074 2321 2524 3505 4980 Size: 54 522 498 1247 203 981 1475 Fragments arranged by size: 1475 1247 981 522 498 203 54 BanII G_rGCy'C Cuts at: 0 80 240 804 940 1148 1555 1724 1837 Size: 80 160 564 136 208 407 169 113 Cuts at: 1837 2058 2248 2423 3583 3760 4138 4178 4380 Size: 221 190 175 1160 177 378 40 202 Cuts at: 4380 4442 4856 4980 Size: 62 414 124 Fragments arranged by size: 1160 564 414 407 378 221 208 202 190 177 175 169 160 136 124 113 80 62 40 BbsI GAAGACnn'nnnn_ Cuts at: 0 353 1868 2888 3471 4662 4980 Size: 353 1515 1020 583 1191 318 Fragments arranged by size: 1515 1191 1020 583 353 318 BbvCI CC'TCA_GC Cuts at: 0 565 4433 4980 Size: 565 3868 547 Fragments arranged by size: 3868 565 547 BglI GCCn_nnn'nGGC Cuts at: 0 205 676 2707 3097 4306 4980 Size: 205 471 2031 390 1209 674 Fragments arranged by size: 2031 1209 674 471 390 205 BglII A'GATC_T Cuts at: 0 584 778 4980 Size: 584 194 4202 Fragments arranged by size: 4202 584 194 BlpI GC'TnA_GC Cuts at: 0 40 3136 3815 4850 4980 Size: 40 3096 679 1035 130 Fragments arranged by size: 3096 1035 679 130 40 Bme1580I G_kGCm'C Cuts at: 0 240 581 624 910 1226 1565 1787 2817 Size: 240 341 43 286 316 339 222 1030 Cuts at: 2817 2857 3518 3827 4178 4599 4755 4761 4840 Size: 40 661 309 351 421 156 6 79 Cuts at: 4840 4910 4980 Size: 70 70 Fragments arranged by size: 1030 661 421 351 341 339 316 309 286 240 222 156 79 70 70 43 40 6 BmrI ACTGGGnnnn_n' Cuts at: 0 83 113 147 780 1391 3881 4980 Size: 83 30 34 633 611 2490 1099 Fragments arranged by size: 2490 1099 633 611 83 34 30 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 348 1095 1142 2425 3330 4132 4980 Size: 348 747 47 1283 905 802 848 Fragments arranged by size: 1283 905 848 802 747 348 47 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 854 905 1003 1373 1593 2314 2571 Size: 472 382 51 98 370 220 721 257 Cuts at: 2571 3190 4127 4438 4980 Size: 619 937 311 542 Fragments arranged by size: 937 721 619 542 472 382 370 311 257 220 98 51 Bpu10I CC'TnA_GC Cuts at: 0 241 565 625 1489 3577 4433 4980 Size: 241 324 60 864 2088 856 547 Fragments arranged by size: 2088 864 856 547 324 241 60 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3232 4980 Size: 3232 1748 BsaBI GATnn'nnATC Cuts at: 0 2359 4980 Size: 2359 2621 BsaHI Gr'CG_yC Cuts at: 0 3045 3638 4980 Size: 3045 593 1342 Fragments arranged by size: 3045 1342 593 BsaWI w'CCGG_w Cuts at: 0 2204 2435 2483 2645 3144 4980 Size: 2204 231 48 162 499 1836 Fragments arranged by size: 2204 1836 499 231 162 48 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 976 1006 1216 1246 1346 1376 2164 2194 Size: 976 30 210 30 100 30 788 30 Cuts at: 2194 3259 3289 4384 4414 4492 4495 4522 4525 Size: 1065 30 1095 30 78 3 27 3 Cuts at: 4525 4980 Size: 455 Fragments arranged by size: 1095 1065 976 788 455 210 100 78 30 30 30 30 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 505 553 684 1936 2188 2311 2989 3644 Size: 505 48 131 1252 252 123 678 655 Cuts at: 3644 4297 4405 4435 4450 4480 4504 4513 4516 Size: 653 108 30 15 30 24 9 3 Cuts at: 4516 4783 4980 Size: 267 197 Fragments arranged by size: 1252 678 655 653 505 267 252 197 131 123 108 48 30 30 24 15 9 3 BseYI C'CCAG_C Cuts at: 0 96 156 289 540 1497 1651 1821 3224 Size: 96 60 133 251 957 154 170 1403 Cuts at: 3224 3321 3394 3616 3959 4171 4177 4203 4444 Size: 97 73 222 343 212 6 26 241 Cuts at: 4444 4566 4782 4856 4980 Size: 122 216 74 124 Fragments arranged by size: 1403 957 343 251 241 222 216 212 170 154 133 124 122 97 96 74 73 60 26 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1534 2850 3550 4594 4980 Size: 1534 1316 700 1044 386 Fragments arranged by size: 1534 1316 1044 700 386 BsiEI CG_ry'CG Cuts at: 0 2150 4980 Size: 2150 2830 BsiHKAI G_wGCw'C Cuts at: 0 836 940 1226 1724 1898 2248 2423 2645 Size: 836 104 286 498 174 350 175 222 Cuts at: 2645 2857 3020 3142 3760 4980 Size: 212 163 122 618 1220 Fragments arranged by size: 1220 836 618 498 350 286 222 212 175 174 163 122 104 BsmI GAATG_Cn' Cuts at: 0 433 2384 2991 4980 Size: 433 1951 607 1989 Fragments arranged by size: 1989 1951 607 433 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3824 4980 Size: 3824 1156 Bsp1286I G_dGCh'C Cuts at: 0 80 240 581 624 804 836 910 940 Size: 80 160 341 43 180 32 74 30 Cuts at: 940 1148 1226 1555 1565 1724 1787 1837 1898 Size: 208 78 329 10 159 63 50 61 Cuts at: 1898 2058 2248 2423 2645 2817 2857 3020 3142 Size: 160 190 175 222 172 40 163 122 Cuts at: 3142 3518 3583 3760 3827 4138 4178 4380 4442 Size: 376 65 177 67 311 40 202 62 Cuts at: 4442 4599 4755 4761 4840 4856 4910 4980 Size: 157 156 6 79 16 54 70 Fragments arranged by size: 376 341 329 311 222 208 202 190 180 177 175 172 163 160 160 159 157 156 122 80 79 78 74 70 67 65 63 62 61 54 50 43 40 40 32 30 16 10 6 BspEI T'CCGG_A Cuts at: 0 2204 2435 2645 3144 4980 Size: 2204 231 210 499 1836 Fragments arranged by size: 2204 1836 499 231 210 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1915 2052 2083 2497 4980 Size: 1915 137 31 414 2483 Fragments arranged by size: 2483 1915 414 137 31 BsrBI CCG'CTC Cuts at: 0 495 2178 4456 4980 Size: 495 1683 2278 524 Fragments arranged by size: 2278 1683 524 495 BsrDI GCAATG_nn' Cuts at: 0 629 665 2261 4980 Size: 629 36 1596 2719 Fragments arranged by size: 2719 1596 629 36 BsrFI r'CCGG_y Cuts at: 0 57 532 1527 3332 4428 4980 Size: 57 475 995 1805 1096 552 Fragments arranged by size: 1805 1096 995 552 475 57 BsrGI T'GTAC_A Cuts at: 0 2687 2939 4980 Size: 2687 252 2041 Fragments arranged by size: 2687 2041 252 BstEII G'GTnAC_C Cuts at: 0 2724 4923 4980 Size: 2724 2199 57 Fragments arranged by size: 2724 2199 57 BstXI CCAn_nnnn'nTGG Cuts at: 0 96 4032 4317 4980 Size: 96 3936 285 663 Fragments arranged by size: 3936 663 285 96 BstYI r'GATC_y Cuts at: 0 584 778 2134 3775 4354 4980 Size: 584 194 1356 1641 579 626 Fragments arranged by size: 1641 1356 626 584 579 194 Bsu36I CC'TnA_GG Cuts at: 0 48 323 749 1037 2705 4980 Size: 48 275 426 288 1668 2275 Fragments arranged by size: 2275 1668 426 288 275 48 BtgI C'CryG_G Cuts at: 0 21 971 1297 1361 2144 2250 2858 4129 Size: 21 950 326 64 783 106 608 1271 Cuts at: 4129 4195 4559 4572 4704 4980 Size: 66 364 13 132 276 Fragments arranged by size: 1271 950 783 608 364 326 276 132 106 66 64 21 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 431 4980 Size: 431 4549 BtsI GCAGTG_nn' Cuts at: 0 358 2337 3534 4980 Size: 358 1979 1197 1446 Fragments arranged by size: 1979 1446 1197 358 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2502 2537 4980 Size: 2502 35 2443 Fragments arranged by size: 2502 2443 35 DraIII CAC_nnn'GTG Cuts at: 0 3726 4980 Size: 3726 1254 DrdI GACnn_nn'nnGTC Cuts at: 0 3858 4921 4980 Size: 3858 1063 59 Fragments arranged by size: 3858 1063 59 EaeI y'GGCC_r Cuts at: 0 212 313 2666 2948 4056 4556 4980 Size: 212 101 2353 282 1108 500 424 Fragments arranged by size: 2353 1108 500 424 282 212 101 EarI CTCTTCn'nnn_ Cuts at: 0 824 1086 1213 1459 1697 1712 2644 4490 Size: 824 262 127 246 238 15 932 1846 Cuts at: 4490 4980 Size: 490 Fragments arranged by size: 1846 932 824 490 262 246 238 127 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2596 4759 4980 Size: 2596 2163 221 Fragments arranged by size: 2596 2163 221 EcoICRI GAG'CTC Cuts at: 0 938 1722 2246 2421 3758 4980 Size: 938 784 524 175 1337 1222 Fragments arranged by size: 1337 1222 938 784 524 175 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 848 854 905 917 1003 1099 1232 Size: 472 376 6 51 12 86 96 133 Cuts at: 1232 1373 1593 2314 2571 2785 3190 3229 3255 Size: 141 220 721 257 214 405 39 26 Cuts at: 3255 3256 4127 4395 4402 4438 4980 Size: 1 871 268 7 36 542 Fragments arranged by size: 871 721 542 472 405 376 268 257 220 214 141 133 96 86 51 39 36 26 12 7 6 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 748 1216 2413 2875 4420 4980 Size: 748 468 1197 462 1545 560 Fragments arranged by size: 1545 1197 748 560 468 462 EcoO109I rG'GnC_Cy Cuts at: 0 109 236 237 307 319 511 600 678 Size: 109 127 1 70 12 192 89 78 Cuts at: 678 1041 1357 1433 1493 1646 1794 2661 3165 Size: 363 316 76 60 153 148 867 504 Cuts at: 3165 3499 3682 3708 3735 3883 4325 4404 4980 Size: 334 183 26 27 148 442 79 576 Fragments arranged by size: 867 576 504 442 363 334 316 192 183 153 148 148 127 109 89 79 78 76 70 60 27 26 12 1 FseI GG_CCGG'CC Cuts at: 0 536 4980 Size: 536 4444 HaeII r_GCGC'y Cuts at: 0 47 1004 4204 4980 Size: 47 957 3200 776 Fragments arranged by size: 3200 957 776 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 93 125 742 774 823 855 1460 1492 Size: 93 32 617 32 49 32 605 32 Cuts at: 1492 2456 2488 4207 4239 4495 4527 4980 Size: 964 32 1719 32 256 32 453 Fragments arranged by size: 1719 964 617 605 453 256 93 49 32 32 32 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1491 3066 3170 3365 3785 4493 4980 Size: 1491 1575 104 195 420 708 487 Fragments arranged by size: 1575 1491 708 487 420 195 104 MscI TGG'CCA Cuts at: 0 2668 2950 4058 4980 Size: 2668 282 1108 922 Fragments arranged by size: 2668 1108 922 282 MslI CAynn'nnrTG Cuts at: 0 3013 4980 Size: 3013 1967 MspA1I CmG'CkG Cuts at: 0 82 1351 2252 2922 3002 3078 3084 3290 Size: 82 1269 901 670 80 76 6 206 Cuts at: 3290 3804 3959 4028 4317 4643 4740 4973 4980 Size: 514 155 69 289 326 97 233 7 Fragments arranged by size: 1269 901 670 514 326 289 233 206 155 97 82 80 76 69 7 6 NaeI GCC'GGC Cuts at: 0 534 3334 4430 4980 Size: 534 2800 1096 550 Fragments arranged by size: 2800 1096 550 534 NcoI C'CATG_G Cuts at: 0 21 971 4980 Size: 21 950 4009 Fragments arranged by size: 4009 950 21 NgoMIV G'CCGG_C Cuts at: 0 532 3332 4428 4980 Size: 532 2800 1096 552 Fragments arranged by size: 2800 1096 552 532 NspI r_CATG'y Cuts at: 0 1817 2559 2929 3927 4980 Size: 1817 742 370 998 1053 Fragments arranged by size: 1817 1053 998 742 370 PasI CC'CwG_GG Cuts at: 0 508 1384 4350 4980 Size: 508 876 2966 630 Fragments arranged by size: 2966 876 630 508 PciI A'CATG_T Cuts at: 0 1813 4980 Size: 1813 3167 PflMI CCAn_nnn'nTGG Cuts at: 0 192 2664 4710 4980 Size: 192 2472 2046 270 Fragments arranged by size: 2472 2046 270 192 PfoI T'CCnGG_A Cuts at: 0 1478 2159 2548 2657 4532 4980 Size: 1478 681 389 109 1875 448 Fragments arranged by size: 1875 1478 681 448 389 109 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 4144 4176 4980 Size: 4144 32 804 Fragments arranged by size: 4144 804 32 PpuMI rG'GwC_Cy Cuts at: 0 109 307 1357 1493 1646 1794 2661 3499 Size: 109 198 1050 136 153 148 867 838 Cuts at: 3499 3735 3883 4404 4980 Size: 236 148 521 576 Fragments arranged by size: 1050 867 838 576 521 236 198 153 148 148 136 109 PshAI GACnn'nnGTC Cuts at: 0 2753 3406 4980 Size: 2753 653 1574 Fragments arranged by size: 2753 1574 653 PspOMI G'GGCC_C Cuts at: 0 236 4174 4980 Size: 236 3938 806 Fragments arranged by size: 3938 806 236 PspXI vC'TCGA_Gb Cuts at: 0 442 4980 Size: 442 4538 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 3349 3381 4980 Size: 3349 32 1599 Fragments arranged by size: 3349 1599 32 PstI C_TGCA'G Cuts at: 0 365 1181 4980 Size: 365 816 3799 Fragments arranged by size: 3799 816 365 PvuII CAG'CTG Cuts at: 0 1351 2922 3084 3959 4028 4317 4740 4980 Size: 1351 1571 162 875 69 289 423 240 Fragments arranged by size: 1571 1351 875 423 289 240 162 69 SacI G_AGCT'C Cuts at: 0 940 1724 2248 2423 3760 4980 Size: 940 784 524 175 1337 1220 Fragments arranged by size: 1337 1220 940 784 524 175 SacII CC_GC'GG Cuts at: 0 2253 4980 Size: 2253 2727 SanDI GG'GwC_CC Cuts at: 0 307 3735 4404 4980 Size: 307 3428 669 576 Fragments arranged by size: 3428 669 576 307 SapI GCTCTTCn'nnn_ Cuts at: 0 824 1712 4980 Size: 824 888 3268 Fragments arranged by size: 3268 888 824 ScaI AGT'ACT Cuts at: 0 3907 4980 Size: 3907 1073 SexAI A'CCwGG_T Cuts at: 0 2720 4980 Size: 2720 2260 SfcI C'TryA_G Cuts at: 0 361 1177 4965 4980 Size: 361 816 3788 15 Fragments arranged by size: 3788 816 361 15 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2707 4980 Size: 2707 2273 SmaI CCC'GGG Cuts at: 0 1438 4980 Size: 1438 3542 SmlI C'TyrA_G Cuts at: 0 442 2002 3247 4627 4980 Size: 442 1560 1245 1380 353 Fragments arranged by size: 1560 1380 1245 442 353 StuI AGG'CCT Cuts at: 0 754 2710 3200 3233 4980 Size: 754 1956 490 33 1747 Fragments arranged by size: 1956 1747 754 490 33 StyI C'CwwG_G Cuts at: 0 21 112 635 971 1058 1319 1949 2499 Size: 21 91 523 336 87 261 630 550 Cuts at: 2499 2876 3686 3704 4166 4980 Size: 377 810 18 462 814 Fragments arranged by size: 814 810 630 550 523 462 377 336 261 91 87 21 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 417 1168 1332 1959 1986 2034 2262 2840 Size: 417 751 164 627 27 48 228 578 Cuts at: 2840 3940 4980 Size: 1100 1040 Fragments arranged by size: 1100 1040 751 627 578 417 228 164 48 27 TatI w'GTAC_w Cuts at: 0 1110 2558 2687 2939 3284 3365 3905 4980 Size: 1110 1448 129 252 345 81 540 1075 Fragments arranged by size: 1448 1110 1075 540 345 252 129 81 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1216 1248 1346 1378 3259 3291 3712 3744 Size: 1216 32 98 32 1881 32 421 32 Cuts at: 3744 3930 3962 4384 4416 4492 4524 4980 Size: 186 32 422 32 76 32 456 Fragments arranged by size: 1881 1216 456 422 421 186 98 76 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 89 3887 4980 Size: 89 3798 1093 Fragments arranged by size: 3798 1093 89 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 978 1065 2031 4051 4707 4980 Size: 978 87 966 2020 656 273 Fragments arranged by size: 2020 978 966 656 273 87 XhoI C'TCGA_G Cuts at: 0 442 4980 Size: 442 4538 XmaI C'CCGG_G Cuts at: 0 1436 4980 Size: 1436 3544 XmnI GAAnn'nnTTC Cuts at: 0 2653 3268 4980 Size: 2653 615 1712 Fragments arranged by size: 2653 1712 615 Enzymes that do cut: AarI AccI AcuI AflII AflIII AhdI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuII SacI SacII SanDI SapI ScaI SexAI SfcI SfiI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AgeI AleI AlfI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI BmgBI BmtI BsaI BsaAI BsiWI BspHI BssHII BssSI BstAPI BstBI BstZ17I ClaI DraI EagI EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PsiI PvuI RsrII SalI SbfI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI ZraI