################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaHI BsaI BseAI BsiWI BsoBI BspDI BspEI BspHI BspMI BsrDI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I ClaI CpoI DraI DraII DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoRI EcoT22I FbaI FspI Hin1I HindIII HpaI KasI KspI MamI MfeI MluI MroI MunI MvnI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 764 GGTACC G^GTACC AcsI 75 GAATTT [GA]^AATT[CT] AflIII 1046 ACATGT A^C[GA][CT]GT AlwI 1257 GATCC GGATC(4/5) AlwNI 505 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 75 GAATTT [GA]^AATT[CT] Asp700I 705 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 764 GGTACC G^GTACC BalI 819 TGGCCA TGG^CCA BanI 764 GGTACC G^G[CT][GA]CC BbvCI 1362 GCTGAGG CCTCAGC(-5/-2) BcnI 1139 CCCGG CC^[GC]GG BfaI 1094 CTAG C^TAG BsaWI 761 TCCGGT [AT]^CCGG[AT] BsiEI 1530 CGGCCG CG[GA][CT]^CG BsmFI 234 GTCCC GGGAC(10/14) BsmI 864 GCATTC GAATGC(1/-1) Bsp1407I 129 TGTACA T^GTACA BspLU11I 1046 ACATGT A^CATGT BsrBI 1605 GAGCGG CCGCTC(-3/-3) BsrGI 129 TGTACA T^GTACA BstEII 1499 GGTCACC G^GT[ACGT]ACC BstNI 1284 CCTGG CC^[AT]GG BstPI 1499 GGTCACC G^GT[ACGT]ACC BstXI 1337 CCACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1256 AGATCC [GA]^GATC[CT] EagI 1530 CGGCCG C^GGCCG EclXI 1530 CGGCCG C^GGCCG Eco52I 1530 CGGCCG C^GGCCG EcoO65I 1499 GGTCACC G^GT[ACGT]ACC EcoRII 1284 CCTGG ^CC[AT]GG EcoRV 160 GATATC GAT^ATC FseI 1531 GGCCGGCC GGCCGG^CC HaeII 281 AGCGCC [GA]GCGC^[CT] HgaI 1275 GCGTC GACGC(5/10) HincII 378 GTTGAC GT[CT]^[GA]AC HindII 378 GTTGAC GT[CT]^[GA]AC KpnI 764 GGTACC GGTAC^C MaeI 1094 CTAG C^TAG MflI 1256 AGATCC [GA]^GATC[CT] MluNI 819 TGGCCA TGG^CCA MscI 819 TGGCCA TGG^CCA MvaI 1284 CCTGG CC^[AT]GG NciI 1139 CCCGG CC^[GC]GG NdeI 353 CATATG CA^TATG NspI 1046 ACATGT [GA]CATG^[CT] SmlI 1185 CTTGAG C^T[CT][GA]AG SspBI 129 TGTACA T^GTACA TaqI 242 TCGA T^CGA TthHB8I 242 TCGA T^CGA XhoII 1256 AGATCC [GA]^GATC[CT] XmnI 705 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AvaII 210 GGACC G^G[AT]CC 1431 GGTCC BbsI 27 GAAGAC GAAGAC(2/6) 709 GTCTTC BglI 821 GCCATGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1138 GCCCGGCTGGC BlpI 894 GCTGAGC GC^T[ACGT]AGC 1602 GCTGAGC BpmI 1055 CTGGAG CTGGAG(16/14) 1384 CTGGAG Bpu1102I 894 GCTGAGC GC^T[ACGT]AGC 1602 GCTGAGC BpuAI 27 GAAGAC GAAGAC(2/6) 709 GTCTTC BsgI 1624 GTGCAG GTGCAG(16/14) 1655 GTGCAG BsmBI 1276 CGTCTC CGTCTC(1/5) 1619 GAGACG BssKI 1139 CCCGG ^CC[ACGT]GG 1284 CCTGG BssSI 933 CACGAG CACGAG(-5/-1) 1291 CACGAG CelII 894 GCTGAGC GC^T[ACGT]AGC 1602 GCTGAGC CfoI 282 GCGC GCG^C 1554 GCGC DpnII 225 GATC ^GATC 1257 GATC HhaI 282 GCGC GCG^C 1554 GCGC HinP1I 282 GCGC G^CGC 1554 GCGC HphI 297 TCACC GGTGA(8/7) 1501 TCACC MboI 225 GATC ^GATC 1257 GATC NdeII 225 GATC ^GATC 1257 GATC NlaIV 764 GGTACC GG[ACGT]^[ACGT]CC 1570 GGCTCC PflMI 553 CCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 714 CCATCCCGTGG SapI 1441 GAAGAGC GCTCTTC(1/4) 1468 GAAGAGC Sau3AI 225 GATC ^GATC 1257 GATC SbfI 1260 CCTGCAGG CCTGCA^GG 1404 CCTGCAGG ScrFI 1139 CCCGG CC^[ACGT]GG 1284 CCTGG Sse8387I 1260 CCTGCAGG CCTGCA^GG 1404 CCTGCAGG StuI 1298 AGGCCT AGG^CCT 1631 AGGCCT TfiI 469 GATTC G^A[AT]TC 1269 GATTC Tsp45I 1165 GTGAC ^GT[GC]AC 1500 GTCAC Van91I 553 CCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 714 CCATCCCGTGG XcmI 1044 CCACATGTCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1073 CCAAGAGTGTTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AfaI AluI AspHI BbvI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrFI BsrI BstF5I Cac8I Cfr10I Cfr13I DdeI DsaI EaeI EarI EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeII MaeIII MboII MnlI MseI MslI MspA1I MspI MwoI NaeI NcoI NgoMIV NlaIII PleI PstI PvuII RsaI Sau96I SfaNI SfcI StyI Tru9I TseI Tsp509I TspRI