(Circular) (Six-base) MAPSORT of: pFN21AB9930.seq Check: 5764 from: 1 to: 6406 pFN21AB9930 6406 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4216 278 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3749 1937 Size: 1812 4594 Acc65I G'GTAC_C Cuts at: 2769 3727 2769 Size: 958 5448 AclI AA'CG_TT Cuts at: 1563 4533 4906 1563 Size: 2970 373 3063 Fragments arranged by size: 3063 2970 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2537 2549 3485 4469 5853 6121 1632 Size: 905 12 936 984 1384 268 1917 Fragments arranged by size: 1917 1384 984 936 905 268 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6209 AflIII A'CryG_T Cuts at: 1203 3051 5305 6282 1203 Size: 1848 2254 977 1327 Fragments arranged by size: 2254 1848 1327 977 AgeI A'CCGG_T Cuts at: 5237 5237 Size: 6406 AhdI GACnn_n'nnGTC Cuts at: 5135 5135 Size: 6406 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6406 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2712 1184 Size: 1528 4878 AlwNI CAG_nnn'CTG Cuts at: 2515 4149 5721 2515 Size: 1634 1572 3200 Fragments arranged by size: 3200 1634 1572 ApaLI G'TGCA_C Cuts at: 4463 5619 4463 Size: 1156 5250 ApoI r'AATT_y Cuts at: 1493 2080 3715 3941 4093 6202 1493 Size: 587 1635 226 152 2109 1697 Fragments arranged by size: 2109 1697 1635 587 226 152 AseI AT'TA_AT Cuts at: 160 1032 4960 160 Size: 872 3928 1606 Fragments arranged by size: 3928 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 AvaI C'yCGr_G Cuts at: 1870 1943 3731 1870 Size: 73 1788 4545 Fragments arranged by size: 4545 1788 73 BamHI G'GATC_C Cuts at: 3736 4103 3736 Size: 367 6039 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2769 3727 5182 618 Size: 325 217 627 982 958 1455 1842 Fragments arranged by size: 1842 1455 982 958 627 325 217 BanII G_rGCy'C Cuts at: 1391 3725 1391 Size: 2334 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2039 2707 928 Size: 971 140 668 4627 Fragments arranged by size: 4627 971 668 140 BbvCI CC'TCA_GC Cuts at: 3368 3368 Size: 6406 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4597 4631 1903 Size: 34 2660 34 3678 Fragments arranged by size: 3678 2660 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4298 5508 4298 Size: 1210 5196 BclI T'GATC_A Cuts at: 1548 1548 Size: 6406 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2832 3149 5017 Size: 107 122 71 631 605 1160 317 1868 Cuts at: 5017 136 Size: 1525 Fragments arranged by size: 1868 1525 1160 631 605 317 122 107 71 BglII A'GATC_T Cuts at: 6401 6401 Size: 6406 BlpI GC'TnA_GC Cuts at: 2900 3608 3823 2900 Size: 708 215 5483 Fragments arranged by size: 5483 708 215 Bme1580I G_kGCm'C Cuts at: 1790 3069 4467 5623 1790 Size: 1279 1398 1156 2573 Fragments arranged by size: 2573 1398 1279 1156 BmgBI CAC'GTC Cuts at: 1318 3567 3628 1318 Size: 2249 61 4096 Fragments arranged by size: 4096 2249 61 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2656 4137 5095 Size: 416 53 83 399 24 1239 1481 958 Cuts at: 5095 442 Size: 1753 Fragments arranged by size: 1753 1481 1239 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6406 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2890 3322 2890 Size: 432 5974 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3081 3410 5066 1239 Size: 1842 329 1656 2579 Fragments arranged by size: 2579 1842 1656 329 Bpu10I CC'TnA_GC Cuts at: 2967 3368 3492 2967 Size: 401 124 5881 Fragments arranged by size: 5881 401 124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3211 4531 5396 5694 3211 Size: 1320 865 298 3923 Fragments arranged by size: 3923 1320 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5069 882 Size: 617 3570 2219 Fragments arranged by size: 3570 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4197 493 Size: 711 2993 2702 Fragments arranged by size: 2993 2702 711 BsaBI GATnn'nnATC Cuts at: 6400 6400 Size: 6406 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4213 4595 5230 275 Size: 53 83 186 936 2680 382 635 1451 Fragments arranged by size: 2680 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2766 4839 5237 5511 5658 6069 2766 Size: 2073 398 274 147 411 3103 Fragments arranged by size: 3103 2073 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3163 3193 1178 Size: 30 165 30 1760 30 4391 Fragments arranged by size: 4391 1760 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2251 2324 2327 2646 3000 1186 Size: 1065 73 3 319 354 4592 Fragments arranged by size: 4592 1065 354 319 73 3 BseYI C'CCAG_C Cuts at: 3309 5609 6105 3309 Size: 2300 496 3610 Fragments arranged by size: 3610 2300 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3650 3681 1163 Size: 573 1914 31 3888 Fragments arranged by size: 3888 1914 573 31 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3538 3856 4617 5645 1540 Size: 275 186 1537 318 761 1028 2301 Fragments arranged by size: 2301 1537 1028 761 318 275 186 BsiHKAI G_wGCw'C Cuts at: 2077 2580 2735 4467 4552 5623 2077 Size: 503 155 1732 85 1071 2860 Fragments arranged by size: 2860 1732 1071 503 155 85 BsmI GAATG_Cn' Cuts at: 2869 3917 4010 2869 Size: 1048 93 5265 Fragments arranged by size: 5265 1048 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3287 3618 3287 Size: 331 6075 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2077 2580 2735 3069 3725 4467 4552 Size: 399 287 503 155 334 656 742 85 Cuts at: 4552 5623 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 656 503 399 334 287 155 85 BspEI T'CCGG_A Cuts at: 6069 6069 Size: 6406 BspHI T'CATG_A Cuts at: 4295 6025 4295 Size: 1730 4676 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3761 844 Size: 2917 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3612 4293 1394 Size: 429 1789 681 3507 Fragments arranged by size: 3507 1789 681 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4901 5075 65 Size: 1183 3653 174 1396 Fragments arranged by size: 3653 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2831 3537 3572 5050 5237 1700 Size: 110 1021 706 35 1478 187 2869 Fragments arranged by size: 2869 1478 1021 706 187 110 35 BsrGI T'GTAC_A Cuts at: 96 2134 96 Size: 2038 4368 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6406 BssSI C'ACGA_G Cuts at: 2938 3296 4466 5478 2938 Size: 358 1170 1012 3866 Fragments arranged by size: 3866 1170 1012 358 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6406 BstBI TT'CG_AA Cuts at: 5210 5210 Size: 6406 BstEII G'GTnAC_C Cuts at: 1184 3504 1184 Size: 2320 4086 BstXI CCAn_nnnn'nTGG Cuts at: 3349 3349 Size: 6406 BstYI r'GATC_y Cuts at: 1972 3261 3736 4103 4488 4505 5957 6043 6055 Size: 1289 475 367 385 17 1452 86 12 Cuts at: 6055 6401 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1289 475 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2037 2724 3039 3526 513 Size: 552 972 687 315 487 3393 Fragments arranged by size: 3393 972 687 552 487 315 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3501 487 Size: 683 56 2275 3392 Fragments arranged by size: 3392 2275 683 56 BtsI GCAGTG_nn' Cuts at: 2789 3922 4716 4736 2789 Size: 1133 794 20 4459 Fragments arranged by size: 4459 1133 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6406 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6371 DraI TTT'AAA Cuts at: 3710 3710 Size: 6406 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5413 809 Size: 507 4097 1802 Fragments arranged by size: 4097 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2824 3535 3539 Size: 54 1425 50 275 18 994 711 4 Cuts at: 3539 3853 4742 6371 8 Size: 314 889 1629 43 Fragments arranged by size: 1629 1425 994 889 711 314 275 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 3535 3853 1537 Size: 275 1723 318 4090 Fragments arranged by size: 4090 1723 318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2042 3441 3468 3669 4336 1353 Size: 689 1399 27 201 667 3423 Fragments arranged by size: 3423 1399 689 667 201 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3275 3368 4993 5367 5513 387 Size: 1064 138 1686 93 1625 374 146 1280 Fragments arranged by size: 1686 1625 1280 1064 374 146 138 93 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2537 2549 3081 3410 3485 4469 5066 Size: 393 905 12 532 329 75 984 597 Cuts at: 5066 5853 6121 1239 Size: 787 268 1524 Fragments arranged by size: 1524 984 905 787 597 532 393 329 268 75 12 EcoRI G'AATT_C Cuts at: 3715 4093 3715 Size: 378 6028 EcoRV GAT'ATC Cuts at: 2167 2167 Size: 6406 FseI GG_CCGG'CC Cuts at: 3541 3541 Size: 6406 FspI TGC'GCA Cuts at: 4912 4912 Size: 6406 HaeII r_GCGC'y Cuts at: 1678 2290 5553 1678 Size: 612 3263 2531 Fragments arranged by size: 3263 2531 612 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3163 3195 3265 3297 5047 Size: 32 135 32 1591 32 70 32 1750 Cuts at: 5047 5079 5121 5153 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1750 1591 135 70 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2385 3750 3996 669 Size: 988 281 447 1365 246 3079 Fragments arranged by size: 3079 1365 988 447 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6406 HpaI GTT'AAC Cuts at: 3996 3996 Size: 6406 KpnI G_GTAC'C Cuts at: 2773 3731 2773 Size: 958 5448 MfeI C'AATT_G Cuts at: 4005 4005 Size: 6406 MluI A'CGCG_T Cuts at: 6282 6282 Size: 6406 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5520 5704 1305 Size: 4215 184 2007 Fragments arranged by size: 4215 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2826 10 Size: 54 1425 343 994 3590 Fragments arranged by size: 3590 1425 994 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2066 2642 3554 4364 4723 4882 518 Size: 648 900 576 912 810 359 159 2042 Fragments arranged by size: 2042 912 900 810 648 576 359 159 MspA1I CmG'CkG Cuts at: 1606 2056 2543 3442 3822 4499 5647 5892 1606 Size: 450 487 899 380 677 1148 245 2120 Fragments arranged by size: 2120 1148 899 677 487 450 380 245 NaeI GCC'GGC Cuts at: 2833 3539 3574 2833 Size: 706 35 5665 Fragments arranged by size: 5665 706 35 NcoI C'CATG_G Cuts at: 513 1065 2037 3039 3526 513 Size: 552 972 1002 487 3393 Fragments arranged by size: 3393 1002 972 552 487 NdeI CA'TA_TG Cuts at: 387 2359 387 Size: 1972 4434 NgoMIV G'CCGG_C Cuts at: 2831 3537 3572 2831 Size: 706 35 5665 Fragments arranged by size: 5665 706 35 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6406 NotI GC'GGCC_GC Cuts at: 3853 3853 Size: 6406 NspI r_CATG'y Cuts at: 1132 1229 3055 3764 5309 1132 Size: 97 1826 709 1545 2229 Fragments arranged by size: 2229 1826 1545 709 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6406 PciI A'CATG_T Cuts at: 3051 5305 3051 Size: 2254 4152 PflMI CCAn_nnn'nTGG Cuts at: 1108 2564 2725 1108 Size: 1456 161 4789 Fragments arranged by size: 4789 1456 161 PmeI GTTT'AAAC Cuts at: 3710 3710 Size: 6406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4475 4507 5988 6020 4475 Size: 32 1481 32 4861 Fragments arranged by size: 4861 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6406 PsiI TTA'TAA Cuts at: 3976 3976 Size: 6406 PstI C_TGCA'G Cuts at: 830 3157 3270 3414 3758 830 Size: 2327 113 144 344 3478 Fragments arranged by size: 3478 2327 344 144 113 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6406 PvuII CAG'CTG Cuts at: 2056 2543 3442 2056 Size: 487 899 5020 Fragments arranged by size: 5020 899 487 SalI G'TCGA_C Cuts at: 1936 3748 1936 Size: 1812 4594 SapI GCTCTTCn'nnn_ Cuts at: 3441 3468 3441 Size: 27 6379 SbfI CC_TGCA'GG Cuts at: 3270 3414 3758 3270 Size: 144 344 5918 Fragments arranged by size: 5918 344 144 ScaI AGT'ACT Cuts at: 4654 6366 4654 Size: 1712 4694 SfcI C'TryA_G Cuts at: 826 1045 3153 3266 3410 3754 4889 5570 5761 Size: 219 2108 113 144 344 1135 681 191 Cuts at: 5761 826 Size: 1471 Fragments arranged by size: 2108 1471 1135 681 344 219 191 144 113 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6406 SmaI CCC'GGG Cuts at: 1872 3733 1872 Size: 1861 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3190 4510 5411 5673 820 Size: 197 926 1247 1320 901 262 1553 Fragments arranged by size: 1553 1320 1247 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4197 493 Size: 3704 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6406 SphI G_CATG'C Cuts at: 1132 3764 1132 Size: 2632 3774 SspI AAT'ATT Cuts at: 5 52 4330 5 Size: 47 4278 2081 Fragments arranged by size: 4278 2081 47 StuI AGG'CCT Cuts at: 1517 3305 3638 1517 Size: 1788 333 4285 Fragments arranged by size: 4285 1788 333 StyI C'CwwG_G Cuts at: 513 1065 2037 3039 3526 3845 513 Size: 552 972 1002 487 319 3074 Fragments arranged by size: 3074 1002 972 552 487 319 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3539 3602 4433 4450 4603 4788 5233 Size: 1255 1959 63 831 17 153 185 445 Cuts at: 5233 325 Size: 1498 Fragments arranged by size: 1959 1498 1255 831 445 185 153 63 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2134 4652 6364 Size: 274 80 33 51 1443 157 2518 1712 Cuts at: 6364 96 Size: 138 Fragments arranged by size: 2518 1712 1443 274 157 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2581 2613 3577 3609 6366 6398 2581 Size: 32 964 32 2757 32 2589 Fragments arranged by size: 2757 2589 964 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6406 XbaI T'CTAG_A Cuts at: 3742 3742 Size: 6406 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3056 3085 3056 Size: 29 6377 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6406 XmaI C'CCGG_G Cuts at: 1870 3731 1870 Size: 1861 4545 XmnI GAAnn'nnTTC Cuts at: 2714 4535 2714 Size: 1821 4585 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4214 276 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI