(Linear) (Six-base) MAPSORT of: KIBB9930.seq Check: 3930 from: 1 to: 1701 KIBB9930.orf With 182 enzymes: SgfI * July 15, 2010 18:32 .. Acc65I G'GTAC_C Cuts at: 0 764 1701 Size: 764 937 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 532 544 1480 1701 Size: 532 12 936 221 Fragments arranged by size: 936 532 221 12 AflIII A'CryG_T Cuts at: 0 1046 1701 Size: 1046 655 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 707 1701 Size: 707 994 AlwNI CAG_nnn'CTG Cuts at: 0 510 1701 Size: 510 1191 ApoI r'AATT_y Cuts at: 0 75 1701 Size: 75 1626 BanI G'GyrC_C Cuts at: 0 764 1701 Size: 764 937 BbsI GAAGACnn'nnnn_ Cuts at: 0 34 702 1701 Size: 34 668 999 Fragments arranged by size: 999 668 34 BbvCI CC'TCA_GC Cuts at: 0 1363 1701 Size: 1363 338 BglI GCCn_nnn'nGGC Cuts at: 0 827 1144 1701 Size: 827 317 557 Fragments arranged by size: 827 557 317 BlpI GC'TnA_GC Cuts at: 0 895 1603 1701 Size: 895 708 98 Fragments arranged by size: 895 708 98 Bme1580I G_kGCm'C Cuts at: 0 1064 1701 Size: 1064 637 BmgBI CAC'GTC Cuts at: 0 1562 1623 1701 Size: 1562 61 78 Fragments arranged by size: 1562 78 61 BmrI ACTGGGnnnn_n' Cuts at: 0 651 1701 Size: 651 1050 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 885 1317 1701 Size: 885 432 384 Fragments arranged by size: 885 432 384 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1076 1405 1701 Size: 1076 329 296 Fragments arranged by size: 1076 329 296 Bpu10I CC'TnA_GC Cuts at: 0 962 1363 1487 1701 Size: 962 401 124 214 Fragments arranged by size: 962 401 214 124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1206 1701 Size: 1206 495 BsaWI w'CCGG_w Cuts at: 0 761 1701 Size: 761 940 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1158 1188 1701 Size: 1158 30 513 Fragments arranged by size: 1158 513 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 246 319 322 641 995 1701 Size: 246 73 3 319 354 706 Fragments arranged by size: 706 354 319 246 73 3 BseYI C'CCAG_C Cuts at: 0 1304 1701 Size: 1304 397 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1645 1676 1701 Size: 1645 31 25 Fragments arranged by size: 1645 31 25 BsiEI CG_ry'CG Cuts at: 0 1533 1701 Size: 1533 168 BsiHKAI G_wGCw'C Cuts at: 0 72 575 730 1701 Size: 72 503 155 971 Fragments arranged by size: 971 503 155 72 BsmI GAATG_Cn' Cuts at: 0 864 1701 Size: 864 837 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1282 1613 1701 Size: 1282 331 88 Fragments arranged by size: 1282 331 88 Bsp1286I G_dGCh'C Cuts at: 0 72 575 730 1064 1701 Size: 72 503 155 334 637 Fragments arranged by size: 637 503 334 155 72 BsrBI CCG'CTC Cuts at: 0 1607 1701 Size: 1607 94 BsrFI r'CCGG_y Cuts at: 0 826 1532 1567 1701 Size: 826 706 35 134 Fragments arranged by size: 826 706 134 35 BsrGI T'GTAC_A Cuts at: 0 129 1701 Size: 129 1572 BssSI C'ACGA_G Cuts at: 0 933 1291 1701 Size: 933 358 410 Fragments arranged by size: 933 410 358 BstEII G'GTnAC_C Cuts at: 0 1499 1701 Size: 1499 202 BstXI CCAn_nnnn'nTGG Cuts at: 0 1344 1701 Size: 1344 357 BstYI r'GATC_y Cuts at: 0 1256 1701 Size: 1256 445 BtgI C'CryG_G Cuts at: 0 32 719 1034 1521 1701 Size: 32 687 315 487 180 Fragments arranged by size: 687 487 315 180 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1496 1701 Size: 1496 205 BtsI GCAGTG_nn' Cuts at: 0 784 1701 Size: 784 917 EaeI y'GGCC_r Cuts at: 0 819 1530 1534 1701 Size: 819 711 4 167 Fragments arranged by size: 819 711 167 4 EagI C'GGCC_G Cuts at: 0 1530 1701 Size: 1530 171 EarI CTCTTCn'nnn_ Cuts at: 0 37 1436 1463 1664 1701 Size: 37 1399 27 201 37 Fragments arranged by size: 1399 201 37 37 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1270 1363 1701 Size: 1270 93 338 Fragments arranged by size: 1270 338 93 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 532 544 1076 1405 1480 1701 Size: 532 12 532 329 75 221 Fragments arranged by size: 532 532 329 221 75 12 EcoRV GAT'ATC Cuts at: 0 162 1701 Size: 162 1539 FseI GG_CCGG'CC Cuts at: 0 1536 1701 Size: 1536 165 HaeII r_GCGC'y Cuts at: 0 285 1701 Size: 285 1416 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1158 1190 1260 1292 1701 Size: 1158 32 70 32 409 Fragments arranged by size: 1158 409 70 32 32 HincII GTy'rAC Cuts at: 0 380 1701 Size: 380 1321 KpnI G_GTAC'C Cuts at: 0 768 1701 Size: 768 933 MscI TGG'CCA Cuts at: 0 821 1701 Size: 821 880 MslI CAynn'nnrTG Cuts at: 0 61 637 1549 1701 Size: 61 576 912 152 Fragments arranged by size: 912 576 152 61 MspA1I CmG'CkG Cuts at: 0 51 538 1437 1701 Size: 51 487 899 264 Fragments arranged by size: 899 487 264 51 NaeI GCC'GGC Cuts at: 0 828 1534 1569 1701 Size: 828 706 35 132 Fragments arranged by size: 828 706 132 35 NcoI C'CATG_G Cuts at: 0 32 1034 1521 1701 Size: 32 1002 487 180 Fragments arranged by size: 1002 487 180 32 NdeI CA'TA_TG Cuts at: 0 354 1701 Size: 354 1347 NgoMIV G'CCGG_C Cuts at: 0 826 1532 1567 1701 Size: 826 706 35 134 Fragments arranged by size: 826 706 134 35 NspI r_CATG'y Cuts at: 0 1050 1701 Size: 1050 651 PciI A'CATG_T Cuts at: 0 1046 1701 Size: 1046 655 PflMI CCAn_nnn'nTGG Cuts at: 0 559 720 1701 Size: 559 161 981 Fragments arranged by size: 981 559 161 PstI C_TGCA'G Cuts at: 0 1152 1265 1409 1701 Size: 1152 113 144 292 Fragments arranged by size: 1152 292 144 113 PvuII CAG'CTG Cuts at: 0 51 538 1437 1701 Size: 51 487 899 264 Fragments arranged by size: 899 487 264 51 SapI GCTCTTCn'nnn_ Cuts at: 0 1436 1463 1701 Size: 1436 27 238 Fragments arranged by size: 1436 238 27 SbfI CC_TGCA'GG Cuts at: 0 1265 1409 1701 Size: 1265 144 292 Fragments arranged by size: 1265 292 144 SfcI C'TryA_G Cuts at: 0 1148 1261 1405 1701 Size: 1148 113 144 296 Fragments arranged by size: 1148 296 144 113 SmlI C'TyrA_G Cuts at: 0 1185 1701 Size: 1185 516 StuI AGG'CCT Cuts at: 0 1300 1633 1701 Size: 1300 333 68 Fragments arranged by size: 1300 333 68 StyI C'CwwG_G Cuts at: 0 32 1034 1521 1701 Size: 32 1002 487 180 Fragments arranged by size: 1002 487 180 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1534 1597 1701 Size: 1534 63 104 Fragments arranged by size: 1534 104 63 TatI w'GTAC_w Cuts at: 0 129 1701 Size: 129 1572 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 576 608 1572 1604 1701 Size: 576 32 964 32 97 Fragments arranged by size: 964 576 97 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1051 1080 1701 Size: 1051 29 621 Fragments arranged by size: 1051 621 29 XmnI GAAnn'nnTTC Cuts at: 0 709 1701 Size: 709 992 Enzymes that do cut: Acc65I AcuI AflIII AlfI AlwNI ApoI BanI BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BsrBI BsrFI BsrGI BssSI BstEII BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI EciI Eco57MI EcoRV FseI HaeII Hin4I HincII KpnI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PciI PflMI PstI PvuII SapI SbfI SfcI SmlI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI BcgI BciVI BclI BfrBI BglII BmtI BsaI BsaAI BsaBI BsaHI BsiWI BspEI BspHI BspMI BsrDI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoO109I EcoRI FalI FspI FspAI HindIII HpaI KasI MfeI MluI MmeI NarI NheI NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI ZraI