################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BcnI BfrI BglI BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraII DrdI DsaI Eam1105I EarI Eco47III EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HindIII HpaI KasI KpnI Ksp632I KspI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AcyI 749 GGCGTC G[GA]^CG[CT]C AfaI 192 GTAC GT^AC AocI 342 CCTGAGG CC^T[ACGT]AGG AspHI 7 GAGCAC G[AT]GC[AT]^C AvaII 674 GGTCC G^G[AT]CC BbsI 337 GAAGAC GAAGAC(2/6) BbvCI 198 GCTGAGG CCTCAGC(-5/-2) BfaI 229 CTAG C^TAG BglII 48 AGATCT A^GATCT BlpI 4 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4 GCTGAGC GC^T[ACGT]AGC BpuAI 337 GAAGAC GAAGAC(2/6) BsaHI 749 GGCGTC G[GA]^CG[CT]C BsaI 162 GGTCTC GGTCTC(1/5) BsiEI 444 CGGCCG CG[GA][CT]^CG BsiHKAI 7 GAGCAC G[AT]GC[AT]^C BsmAI 163 GTCTC GTCTC(1/5) BsmFI 629 GTCCC GGGAC(10/14) BsrDI 678 CATTGC GCAATG(2/0) BstYI 48 AGATCT [GA]^GATC[CT] Bsu36I 342 CCTGAGG CC^T[ACGT]AGG CelII 4 GCTGAGC GC^T[ACGT]AGC CfoI 205 GCGC GCG^C Cfr13I 674 GGTCC G^G[ACGT]CC DraIII 640 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 444 CGGCCG [CT]^GGCC[GA] EagI 444 CGGCCG C^GGCCG EclXI 444 CGGCCG C^GGCCG Eco52I 444 CGGCCG C^GGCCG Eco81I 342 CCTGAGG CC^T[ACGT]AGG EcoT14I 265 CCAAGG C^C[AT][AT]GG HaeII 204 GGCGCT [GA]GCGC^[CT] HaeIII 445 GGCC GG^CC HapII 443 CCGG C^CGG HhaI 205 GCGC GCG^C Hin1I 749 GGCGTC G[GA]^CG[CT]C HinP1I 205 GCGC G^CGC HincII 514 GTCAAC GT[CT]^[GA]AC HindII 514 GTCAAC GT[CT]^[GA]AC HpaII 443 CCGG C^CGG HphI 624 GGTGA GGTGA(8/7) MaeI 229 CTAG C^TAG MflI 48 AGATCT [GA]^GATC[CT] MslI 766 CATAAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 827 CCGCTG C[AC]G^C[GT]G MspI 443 CCGG C^CGG RsaI 192 GTAC GT^AC Sau96I 674 GGTCC G^G[ACGT]CC SfaNI 794 GCATC GCATC(5/9) StyI 265 CCAAGG C^C[AT][AT]GG Tsp509I 106 AATT ^AATT XhoII 48 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AciI 447 CCGC CCGC(-3/-1) 827 CCGC BclI 786 TGATCA T^GATCA 815 TGATCA BpmI 94 CTCCAG CTGGAG(16/14) 112 CTCCAG BseRI 614 CTCCTC GAGGAG(10/8) 345 GAGGAG FbaI 786 TGATCA T^GATCA 815 TGATCA HgaI 416 GCGTC GACGC(5/10) 750 GCGTC PleI 612 GACTC GAGTC(4/5) 627 GAGTC TfiI 508 GAATC G^A[AT]TC 729 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AluI BanI BanII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HinfI ItaI MaeII MboI MboII MnlI MvaI MwoI NdeII NlaIV Sau3AI ScrFI TaqI TseI TspRI TthHB8I