(Circular) (Six-base) MAPSORT of: pFN21AE1225.seq Check: 2758 from: 1 to: 5566 pFN21AE1225 5566 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3376 278 Size: 53 83 186 936 1840 2468 Fragments arranged by size: 2468 1840 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2909 1937 Size: 972 4594 Acc65I G'GTAC_C Cuts at: 2887 2887 Size: 5566 AclI AA'CG_TT Cuts at: 1563 3693 4066 1563 Size: 2130 373 3063 Fragments arranged by size: 3063 2130 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2361 2532 3629 5013 5281 1632 Size: 729 171 1097 1384 268 1917 Fragments arranged by size: 1917 1384 1097 729 268 171 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5369 AflIII A'CryG_T Cuts at: 1203 4465 5442 1203 Size: 3262 977 1327 Fragments arranged by size: 3262 1327 977 AgeI A'CCGG_T Cuts at: 4397 4397 Size: 5566 AhdI GACnn_n'nnGTC Cuts at: 4295 4295 Size: 5566 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5566 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5566 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2285 2317 2285 Size: 32 5534 AlwNI CAG_nnn'CTG Cuts at: 3309 4881 3309 Size: 1572 3994 ApaLI G'TGCA_C Cuts at: 3623 4779 3623 Size: 1156 4410 ApoI r'AATT_y Cuts at: 1493 2875 3101 3253 5362 1493 Size: 1382 226 152 2109 1697 Fragments arranged by size: 2109 1697 1382 226 152 AseI AT'TA_AT Cuts at: 160 1032 4120 160 Size: 872 3088 1606 Fragments arranged by size: 3088 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5566 AvaI C'yCGr_G Cuts at: 1870 1943 2891 1870 Size: 73 948 4545 Fragments arranged by size: 4545 948 73 BamHI G'GATC_C Cuts at: 2896 3263 2896 Size: 367 5199 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2429 2477 2709 2887 4342 Size: 325 217 627 642 48 232 178 1455 Cuts at: 4342 618 Size: 1842 Fragments arranged by size: 1842 1455 642 627 325 232 217 178 48 BanII G_rGCy'C Cuts at: 1391 2130 2241 2751 2885 1391 Size: 739 111 510 134 4072 Fragments arranged by size: 4072 739 510 134 111 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2349 928 Size: 971 450 4145 Fragments arranged by size: 4145 971 450 BbvCI CC'TCA_GC Cuts at: 2204 2204 Size: 5566 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3757 3791 1903 Size: 34 1820 34 3678 Fragments arranged by size: 3678 1820 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3458 4668 3458 Size: 1210 4356 BclI T'GATC_A Cuts at: 1548 2791 2820 1548 Size: 1243 29 4294 Fragments arranged by size: 4294 1243 29 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4177 136 Size: 107 122 71 631 605 2505 1525 Fragments arranged by size: 2505 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2053 5561 2053 Size: 3508 2058 BlpI GC'TnA_GC Cuts at: 2010 2983 2010 Size: 973 4593 Bme1580I G_kGCm'C Cuts at: 1790 2432 2480 2845 3627 4783 1790 Size: 642 48 365 782 1156 2573 Fragments arranged by size: 2573 1156 782 642 365 48 BmgBI CAC'GTC Cuts at: 1318 2473 1318 Size: 1155 4411 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2470 2632 2840 Size: 416 53 83 399 24 1053 162 208 Cuts at: 2840 3297 4255 442 Size: 457 958 1753 Fragments arranged by size: 1753 1053 958 457 416 399 208 162 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5566 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 2102 4226 1239 Size: 845 18 2124 2579 Fragments arranged by size: 2579 2124 845 18 Bpu10I CC'TnA_GC Cuts at: 2204 2204 Size: 5566 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3691 4556 4854 3691 Size: 865 298 4403 Fragments arranged by size: 4403 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2173 4229 882 Size: 617 674 2056 2219 Fragments arranged by size: 2219 2056 674 617 BsaAI yAC'GTr Cuts at: 493 1204 3357 493 Size: 711 2153 2702 Fragments arranged by size: 2702 2153 711 BsaBI GATnn'nnATC Cuts at: 5560 5560 Size: 5566 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2755 3373 3755 4390 Size: 53 83 186 936 1222 618 382 635 Cuts at: 4390 275 Size: 1451 Fragments arranged by size: 1451 1222 936 635 618 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3999 4397 4671 4818 5229 3999 Size: 398 274 147 411 4336 Fragments arranged by size: 4336 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5341 Fragments arranged by size: 5341 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2365 2610 1186 Size: 1179 245 4142 Fragments arranged by size: 4142 1179 245 BseYI C'CCAG_C Cuts at: 4769 5265 4769 Size: 496 5070 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4993 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2452 3016 3777 4805 1540 Size: 275 186 451 564 761 1028 2301 Fragments arranged by size: 2301 1028 761 564 451 275 186 BsiHKAI G_wGCw'C Cuts at: 2016 3627 3712 4783 2016 Size: 1611 85 1071 2799 Fragments arranged by size: 2799 1611 1071 85 BsmI GAATG_Cn' Cuts at: 3077 3170 3077 Size: 93 5473 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2130 2241 2432 2480 2751 2845 Size: 399 226 114 111 191 48 271 94 Cuts at: 2845 2885 3627 3712 4783 1391 Size: 40 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 271 226 191 114 111 94 85 48 40 BspEI T'CCGG_A Cuts at: 5229 5229 Size: 5566 BspHI T'CATG_A Cuts at: 3455 5185 3455 Size: 1730 3836 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2921 844 Size: 2077 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3453 1394 Size: 429 1630 3507 Fragments arranged by size: 3507 1630 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2682 4061 4235 65 Size: 1183 1434 1379 174 1396 Fragments arranged by size: 1434 1396 1379 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4210 4397 1700 Size: 110 2400 187 2869 Fragments arranged by size: 2869 2400 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5566 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5566 BssSI C'ACGA_G Cuts at: 3626 4638 3626 Size: 1012 4554 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5566 BstBI TT'CG_AA Cuts at: 4370 4370 Size: 5566 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5566 BstYI r'GATC_y Cuts at: 1972 2053 2896 3263 3648 3665 5117 5203 5215 Size: 81 843 367 385 17 1452 86 12 Cuts at: 5215 5561 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 843 385 367 346 86 81 17 12 Bsu36I CC'TnA_GG Cuts at: 2348 2348 Size: 5566 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4075 Fragments arranged by size: 4075 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4827 Fragments arranged by size: 4827 683 56 BtsI GCAGTG_nn' Cuts at: 2409 3082 3876 3896 2409 Size: 673 794 20 4079 Fragments arranged by size: 4079 794 673 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5566 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5531 DraI TTT'AAA Cuts at: 2870 2870 Size: 5566 DraIII CAC_nnn'GTG Cuts at: 2650 2650 Size: 5566 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4573 809 Size: 507 3257 1802 Fragments arranged by size: 3257 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2449 3013 3902 Size: 54 1425 50 275 18 619 564 889 Cuts at: 3902 5531 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 619 564 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2449 3013 1537 Size: 275 637 564 4090 Fragments arranged by size: 4090 637 564 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3496 1353 Size: 2143 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4153 4527 4673 387 Size: 1064 138 2564 374 146 1280 Fragments arranged by size: 2564 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2102 2361 2532 3629 4226 5013 Size: 393 452 18 259 171 1097 597 787 Cuts at: 5013 5281 1239 Size: 268 1524 Fragments arranged by size: 1524 1097 787 597 452 393 268 259 171 18 EcoRI G'AATT_C Cuts at: 2875 3253 2875 Size: 378 5188 FspI TGC'GCA Cuts at: 4072 4072 Size: 5566 HaeII r_GCGC'y Cuts at: 1678 2213 4713 1678 Size: 535 2500 2531 Fragments arranged by size: 2531 2500 535 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2025 2057 4207 4239 4281 Size: 32 135 32 453 32 2150 32 42 Cuts at: 4281 4313 1373 Size: 32 2626 Fragments arranged by size: 2626 2150 453 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2521 2910 3156 669 Size: 988 281 583 389 246 3079 Fragments arranged by size: 3079 988 583 389 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5566 HpaI GTT'AAC Cuts at: 3156 3156 Size: 5566 KpnI G_GTAC'C Cuts at: 2891 2891 Size: 5566 MfeI C'AATT_G Cuts at: 3165 3165 Size: 5566 MluI A'CGCG_T Cuts at: 5442 5442 Size: 5566 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4680 4864 1305 Size: 3375 184 2007 Fragments arranged by size: 3375 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3744 Fragments arranged by size: 3744 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2775 3524 3883 4042 518 Size: 648 1609 749 359 159 2042 Fragments arranged by size: 2042 1609 749 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2834 2982 3659 4807 5052 1606 Size: 1228 148 677 1148 245 2120 Fragments arranged by size: 2120 1228 1148 677 245 148 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4075 Fragments arranged by size: 4075 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5566 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5566 NotI GC'GGCC_GC Cuts at: 3013 3013 Size: 5566 NspI r_CATG'y Cuts at: 1132 1229 2924 4469 1132 Size: 97 1695 1545 2229 Fragments arranged by size: 2229 1695 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5566 PciI A'CATG_T Cuts at: 4465 4465 Size: 5566 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5566 PfoI T'CCnGG_A Cuts at: 2661 2661 Size: 5566 PmeI GTTT'AAAC Cuts at: 2870 2870 Size: 5566 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2797 2829 3635 3667 5148 5180 2797 Size: 32 806 32 1481 32 3183 Fragments arranged by size: 3183 1481 806 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5566 PsiI TTA'TAA Cuts at: 3136 3136 Size: 5566 PstI C_TGCA'G Cuts at: 830 2918 830 Size: 2088 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5566 SalI G'TCGA_C Cuts at: 1936 2908 1936 Size: 972 4594 SbfI CC_TGCA'GG Cuts at: 2918 2918 Size: 5566 ScaI AGT'ACT Cuts at: 3814 5526 3814 Size: 1712 3854 SfcI C'TryA_G Cuts at: 826 1045 2914 4049 4730 4921 826 Size: 219 1869 1135 681 191 1471 Fragments arranged by size: 1869 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5566 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5566 SmaI CCC'GGG Cuts at: 1872 2893 1872 Size: 1021 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3670 4571 4833 820 Size: 197 926 1727 901 262 1553 Fragments arranged by size: 1727 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3357 493 Size: 2864 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5566 SphI G_CATG'C Cuts at: 1132 2924 1132 Size: 1792 3774 SspI AAT'ATT Cuts at: 5 52 3490 5 Size: 47 3438 2081 Fragments arranged by size: 3438 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5566 StyI C'CwwG_G Cuts at: 513 1065 2004 2270 3005 513 Size: 552 939 266 735 3074 Fragments arranged by size: 3074 939 735 552 266 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2617 2697 3593 3610 3763 3948 4393 Size: 1255 1037 80 896 17 153 185 445 Cuts at: 4393 325 Size: 1498 Fragments arranged by size: 1498 1255 1037 896 445 185 153 80 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2196 3812 5524 Size: 274 80 33 51 1443 219 1616 1712 Cuts at: 5524 96 Size: 138 Fragments arranged by size: 1712 1616 1443 274 219 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5526 5558 5526 Size: 32 5534 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5566 XbaI T'CTAG_A Cuts at: 2902 2902 Size: 5566 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5566 XmaI C'CCGG_G Cuts at: 1870 2891 1870 Size: 1021 4545 XmnI GAAnn'nnTTC Cuts at: 3695 3695 Size: 5566 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3374 276 Size: 53 83 186 936 1840 2468 Fragments arranged by size: 2468 1840 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI