(Circular) (Six-base) MAPSORT of: pFN21AE5639.seq Check: 2290 from: 1 to: 6391 pFN21AE5639 6391 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2530 4201 278 Size: 53 83 186 936 994 1671 2468 Fragments arranged by size: 2468 1671 994 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3734 1937 Size: 1797 4594 Acc65I G'GTAC_C Cuts at: 3712 3712 Size: 6391 AclI AA'CG_TT Cuts at: 1563 4518 4891 1563 Size: 2955 373 3063 Fragments arranged by size: 3063 2955 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2708 3090 3273 3324 4454 5838 6106 1632 Size: 1076 382 183 51 1130 1384 268 1917 Fragments arranged by size: 1917 1384 1130 1076 382 268 183 51 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6194 AflIII A'CryG_T Cuts at: 1203 5290 6267 1203 Size: 4087 977 1327 Fragments arranged by size: 4087 1327 977 AgeI A'CCGG_T Cuts at: 5222 5222 Size: 6391 AhdI GACnn_n'nnGTC Cuts at: 5120 5120 Size: 6391 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6391 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6391 AlwNI CAG_nnn'CTG Cuts at: 2156 2176 2395 2417 2647 2770 2854 2971 3001 Size: 20 219 22 230 123 84 117 30 Cuts at: 3001 3581 4134 5706 2156 Size: 580 553 1572 2841 Fragments arranged by size: 2841 1572 580 553 230 219 123 117 84 30 22 20 ApaI G_GGCC'C Cuts at: 2024 2210 2267 3057 3079 2024 Size: 186 57 790 22 5336 Fragments arranged by size: 5336 790 186 57 22 ApaLI G'TGCA_C Cuts at: 4448 5604 4448 Size: 1156 5235 ApoI r'AATT_y Cuts at: 1493 3700 3926 4078 6187 1493 Size: 2207 226 152 2109 1697 Fragments arranged by size: 2207 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4945 160 Size: 872 3913 1606 Fragments arranged by size: 3913 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6391 AvaI C'yCGr_G Cuts at: 1870 1943 2465 3716 1870 Size: 73 522 1251 4545 Fragments arranged by size: 4545 1251 522 73 BamHI G'GATC_C Cuts at: 3721 4088 3721 Size: 367 6024 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3475 3712 5167 618 Size: 325 217 627 1688 237 1455 1842 Fragments arranged by size: 1842 1688 1455 627 325 237 217 BanII G_rGCy'C Cuts at: 1391 2024 2210 2267 2380 2517 3057 3079 3406 Size: 633 186 57 113 137 540 22 327 Cuts at: 3406 3710 1391 Size: 304 4072 Fragments arranged by size: 4072 633 540 327 304 186 137 113 57 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2460 2696 928 Size: 971 561 236 4623 Fragments arranged by size: 4623 971 561 236 BbvCI CC'TCA_GC Cuts at: 2418 2574 2418 Size: 156 6235 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4582 4616 1903 Size: 34 2645 34 3678 Fragments arranged by size: 3678 2645 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4283 5493 4283 Size: 1210 5181 BclI T'GATC_A Cuts at: 1548 1548 Size: 6391 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2747 5002 136 Size: 107 122 71 631 605 1075 2255 1525 Fragments arranged by size: 2255 1525 1075 631 605 122 107 71 BglII A'GATC_T Cuts at: 2604 6386 2604 Size: 3782 2609 BlpI GC'TnA_GC Cuts at: 2492 2516 3808 2492 Size: 24 1292 5075 Fragments arranged by size: 5075 1292 24 Bme1580I G_kGCm'C Cuts at: 1790 2024 2210 2267 3057 3079 4452 5608 1790 Size: 234 186 57 790 22 1373 1156 2573 Fragments arranged by size: 2573 1373 1156 790 234 186 57 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6391 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3494 4122 5080 Size: 416 53 83 399 24 2077 628 958 Cuts at: 5080 442 Size: 1753 Fragments arranged by size: 2077 1753 958 628 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6391 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2456 2522 2531 2699 2729 3029 3044 Size: 968 249 66 9 168 30 300 15 Cuts at: 3044 3420 3435 3446 3644 3659 3669 5051 1239 Size: 376 15 11 198 15 10 1382 2579 Fragments arranged by size: 2579 1382 968 376 300 249 198 168 66 30 15 15 15 11 10 9 Bpu10I CC'TnA_GC Cuts at: 2418 2574 2418 Size: 156 6235 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4516 5381 5679 4516 Size: 865 298 5228 Fragments arranged by size: 5228 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2354 3218 3436 5054 882 Size: 617 855 864 218 1618 2219 Fragments arranged by size: 2219 1618 864 855 617 218 BsaAI yAC'GTr Cuts at: 493 1204 4182 493 Size: 711 2978 2702 Fragments arranged by size: 2978 2702 711 BsaBI GATnn'nnATC Cuts at: 6385 6385 Size: 6391 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2527 4198 4580 5215 Size: 53 83 186 936 994 1671 382 635 Cuts at: 5215 275 Size: 1451 Fragments arranged by size: 1671 1451 994 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4824 5222 5496 5643 6054 4824 Size: 398 274 147 411 5161 Fragments arranged by size: 5161 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6166 Fragments arranged by size: 6166 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2084 2117 2372 2435 2591 2615 2687 2813 Size: 898 33 255 63 156 24 72 126 Cuts at: 2813 3041 3368 3523 1186 Size: 228 327 155 4054 Fragments arranged by size: 4054 898 327 255 228 156 155 126 72 63 33 24 BseYI C'CCAG_C Cuts at: 2080 2388 2637 3078 3121 3259 3263 3574 5594 Size: 308 249 441 43 138 4 311 2020 Cuts at: 5594 6090 2080 Size: 496 2381 Fragments arranged by size: 2381 2020 496 441 311 308 249 138 43 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5818 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3841 4602 5630 1540 Size: 275 186 1840 761 1028 2301 Fragments arranged by size: 2301 1840 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2517 4452 4537 5608 2517 Size: 1935 85 1071 3300 Fragments arranged by size: 3300 1935 1071 85 BsmI GAATG_Cn' Cuts at: 2091 2548 3902 3995 2091 Size: 457 1354 93 4487 Fragments arranged by size: 4487 1354 457 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2210 2267 2380 2517 3057 3079 Size: 399 234 186 57 113 137 540 22 Cuts at: 3079 3406 3710 4452 4537 5608 1391 Size: 327 304 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 540 399 327 304 234 186 137 113 85 57 22 BspEI T'CCGG_A Cuts at: 6054 6054 Size: 6391 BspHI T'CATG_A Cuts at: 4280 6010 4280 Size: 1730 4661 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2019 2496 3454 3746 844 Size: 1175 477 958 292 3489 Fragments arranged by size: 3489 1175 958 477 292 BsrBI CCG'CTC Cuts at: 1394 1823 2062 2824 4278 1394 Size: 429 239 762 1454 3507 Fragments arranged by size: 3507 1454 762 429 239 BsrDI GCAATG_nn' Cuts at: 65 1248 4886 5060 65 Size: 1183 3638 174 1396 Fragments arranged by size: 3638 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2089 5035 5222 1700 Size: 110 279 2946 187 2869 Fragments arranged by size: 2946 2869 279 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6391 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6391 BssSI C'ACGA_G Cuts at: 4451 5463 4451 Size: 1012 5379 BstAPI GCAn_nnn'nTGC Cuts at: 794 2647 794 Size: 1853 4538 BstBI TT'CG_AA Cuts at: 2127 5195 2127 Size: 3068 3323 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6391 BstXI CCAn_nnnn'nTGG Cuts at: 2245 2665 2707 3330 2245 Size: 420 42 623 5306 Fragments arranged by size: 5306 623 420 42 BstYI r'GATC_y Cuts at: 1972 2604 3721 4088 4473 4490 5942 6028 6040 Size: 632 1117 367 385 17 1452 86 12 Cuts at: 6040 6386 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1117 632 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2016 2067 2268 2016 Size: 51 201 6139 Fragments arranged by size: 6139 201 51 BtgI C'CryG_G Cuts at: 513 1065 2004 2664 2859 513 Size: 552 939 660 195 4045 Fragments arranged by size: 4045 939 660 552 195 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2961 3102 487 Size: 683 56 1735 141 3776 Fragments arranged by size: 3776 1735 683 141 56 BtsI GCAGTG_nn' Cuts at: 2037 3209 3907 4701 4721 2037 Size: 1172 698 794 20 3707 Fragments arranged by size: 3707 1172 794 698 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6391 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6356 DraI TTT'AAA Cuts at: 3695 3695 Size: 6391 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5398 809 Size: 507 4082 1802 Fragments arranged by size: 4082 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2235 2697 2856 Size: 54 1425 50 275 18 405 462 159 Cuts at: 2856 3838 4727 6356 8 Size: 982 889 1629 43 Fragments arranged by size: 1629 1425 982 889 462 405 275 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3838 1537 Size: 275 2026 4090 Fragments arranged by size: 4090 2026 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2349 3082 3337 3345 4321 1353 Size: 996 733 255 8 976 3423 Fragments arranged by size: 3423 996 976 733 255 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2765 4978 5352 5498 387 Size: 1064 138 1176 2213 374 146 1280 Fragments arranged by size: 2213 1280 1176 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2515 2515 Size: 6391 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2456 2522 2531 2699 2708 2729 Size: 393 575 249 66 9 168 9 21 Cuts at: 2729 3029 3044 3090 3273 3324 3420 3435 3446 Size: 300 15 46 183 51 96 15 11 Cuts at: 3446 3644 3659 3669 4454 5051 5838 6106 1239 Size: 198 15 10 785 597 787 268 1524 Fragments arranged by size: 1524 787 785 597 575 393 300 268 249 198 183 168 96 66 51 46 21 15 15 15 11 10 9 9 EcoNI CCTnn'n_nnAGG Cuts at: 2015 2597 3306 2015 Size: 582 709 5100 Fragments arranged by size: 5100 709 582 EcoO109I rG'GnC_Cy Cuts at: 2020 2206 2207 2263 2264 2506 2644 2683 3053 Size: 186 1 56 1 242 138 39 370 Cuts at: 3053 3075 3513 2020 Size: 22 438 4898 Fragments arranged by size: 4898 438 370 242 186 138 56 39 22 1 1 EcoRI G'AATT_C Cuts at: 3700 4078 3700 Size: 378 6013 FspI TGC'GCA Cuts at: 4897 4897 Size: 6391 HaeII r_GCGC'y Cuts at: 1678 5538 1678 Size: 3860 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2567 2596 2599 2628 2773 Size: 32 135 32 995 29 3 29 145 Cuts at: 2773 2805 2910 2942 3233 3265 3562 3594 5032 Size: 32 105 32 291 32 297 32 1438 Cuts at: 5032 5064 5106 5138 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1438 995 297 291 145 135 105 42 32 32 32 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 2330 3336 3735 3981 669 Size: 988 281 392 1006 399 246 3079 Fragments arranged by size: 3079 1006 988 399 392 281 246 HindIII A'AGCT_T Cuts at: 748 2130 748 Size: 1382 5009 HpaI GTT'AAC Cuts at: 3981 3981 Size: 6391 KpnI G_GTAC'C Cuts at: 3716 3716 Size: 6391 MfeI C'AATT_G Cuts at: 3990 3990 Size: 6391 MluI A'CGCG_T Cuts at: 6267 6267 Size: 6391 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3204 5505 5689 1305 Size: 1899 2301 184 2007 Fragments arranged by size: 2301 2007 1899 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2237 2699 2858 10 Size: 54 1425 343 405 462 159 3543 Fragments arranged by size: 3543 1425 462 405 343 159 54 MslI CAynn'nnrTG Cuts at: 518 1166 2404 3328 4349 4708 4867 518 Size: 648 1238 924 1021 359 159 2042 Fragments arranged by size: 2042 1238 1021 924 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2483 2557 2573 3034 3082 3263 3544 3807 Size: 877 74 16 461 48 181 281 263 Cuts at: 3807 4484 5632 5877 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 877 677 461 281 263 245 181 74 48 16 NcoI C'CATG_G Cuts at: 513 1065 2004 2664 2859 513 Size: 552 939 660 195 4045 Fragments arranged by size: 4045 939 660 552 195 NdeI CA'TA_TG Cuts at: 387 387 Size: 6391 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6391 NotI GC'GGCC_GC Cuts at: 3838 3838 Size: 6391 NruI TCG'CGA Cuts at: 2946 2946 Size: 6391 NspI r_CATG'y Cuts at: 1132 1229 3749 5294 1132 Size: 97 2520 1545 2229 Fragments arranged by size: 2520 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6391 PciI A'CATG_T Cuts at: 5290 5290 Size: 6391 PflMI CCAn_nnn'nTGG Cuts at: 1108 2176 2233 2971 1108 Size: 1068 57 738 4528 Fragments arranged by size: 4528 1068 738 57 PfoI T'CCnGG_A Cuts at: 2055 2055 Size: 6391 PmeI GTTT'AAAC Cuts at: 3695 3695 Size: 6391 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4460 4492 5973 6005 4460 Size: 32 1481 32 4846 Fragments arranged by size: 4846 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2506 2683 2506 Size: 177 6214 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6391 PsiI TTA'TAA Cuts at: 3961 3961 Size: 6391 PspOMI G'GGCC_C Cuts at: 2020 2206 2263 3053 3075 2020 Size: 186 57 790 22 5336 Fragments arranged by size: 5336 790 186 57 22 PstI C_TGCA'G Cuts at: 830 2556 2652 2796 3743 830 Size: 1726 96 144 947 3478 Fragments arranged by size: 3478 1726 947 144 96 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6391 PvuII CAG'CTG Cuts at: 2483 2573 3034 3082 3263 2483 Size: 90 461 48 181 5611 Fragments arranged by size: 5611 461 181 90 48 SacI G_AGCT'C Cuts at: 2517 2517 Size: 6391 SalI G'TCGA_C Cuts at: 1936 3733 1936 Size: 1797 4594 SbfI CC_TGCA'GG Cuts at: 3743 3743 Size: 6391 ScaI AGT'ACT Cuts at: 4639 6351 4639 Size: 1712 4679 SfcI C'TryA_G Cuts at: 826 1045 2552 2648 2792 3739 4874 5555 5746 Size: 219 1507 96 144 947 1135 681 191 Cuts at: 5746 826 Size: 1471 Fragments arranged by size: 1507 1471 1135 947 681 219 191 144 96 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6391 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6391 SmaI CCC'GGG Cuts at: 1872 3718 1872 Size: 1846 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4495 5396 5658 820 Size: 197 926 2552 901 262 1553 Fragments arranged by size: 2552 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4182 493 Size: 3689 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6391 SphI G_CATG'C Cuts at: 1132 3749 1132 Size: 2617 3774 SspI AAT'ATT Cuts at: 5 52 4315 5 Size: 47 4263 2081 Fragments arranged by size: 4263 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6391 StyI C'CwwG_G Cuts at: 513 1065 2004 2593 2658 2664 2859 3830 513 Size: 552 939 589 65 6 195 971 3074 Fragments arranged by size: 3074 971 939 589 552 195 65 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2018 2651 3116 4418 4435 4588 4773 Size: 1255 438 633 465 1302 17 153 185 Cuts at: 4773 5218 325 Size: 445 1498 Fragments arranged by size: 1498 1302 1255 633 465 445 438 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4637 6349 96 Size: 274 80 33 51 1443 2660 1712 138 Fragments arranged by size: 2660 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3547 3579 6351 6383 3547 Size: 32 2772 32 3555 Fragments arranged by size: 3555 2772 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6391 XbaI T'CTAG_A Cuts at: 3727 3727 Size: 6391 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2281 3645 2281 Size: 1364 5027 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6391 XmaI C'CCGG_G Cuts at: 1870 3716 1870 Size: 1846 4545 XmnI GAAnn'nnTTC Cuts at: 4520 4520 Size: 6391 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2528 4199 276 Size: 53 83 186 936 994 1671 2468 Fragments arranged by size: 2468 1671 994 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI