(Linear) (Six-base) MAPSORT of: KIEE5639.seq Check: 7603 from: 1 to: 1686 KIEE5639.orf With 182 enzymes: SgfI * December 5, 2014 13:04 .. AatII G_ACGT'C Cuts at: 0 525 1686 Size: 525 1161 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 703 1085 1268 1319 1686 Size: 703 382 183 51 367 Fragments arranged by size: 703 382 367 183 51 AlwNI CAG_nnn'CTG Cuts at: 0 151 171 390 412 642 765 849 966 Size: 151 20 219 22 230 123 84 117 Cuts at: 966 996 1576 1686 Size: 30 580 110 Fragments arranged by size: 580 230 219 151 123 117 110 84 30 22 20 ApaI G_GGCC'C Cuts at: 0 19 205 262 1052 1074 1686 Size: 19 186 57 790 22 612 Fragments arranged by size: 790 612 186 57 22 19 AvaI C'yCGr_G Cuts at: 0 460 1686 Size: 460 1226 BanI G'GyrC_C Cuts at: 0 1470 1686 Size: 1470 216 BanII G_rGCy'C Cuts at: 0 19 205 262 375 512 1052 1074 1401 Size: 19 186 57 113 137 540 22 327 Cuts at: 1401 1686 Size: 285 Fragments arranged by size: 540 327 285 186 137 113 57 22 19 BbsI GAAGACnn'nnnn_ Cuts at: 0 455 691 1686 Size: 455 236 995 Fragments arranged by size: 995 455 236 BbvCI CC'TCA_GC Cuts at: 0 413 569 1686 Size: 413 156 1117 Fragments arranged by size: 1117 413 156 BglI GCCn_nnn'nGGC Cuts at: 0 742 1686 Size: 742 944 BglII A'GATC_T Cuts at: 0 599 1686 Size: 599 1087 BlpI GC'TnA_GC Cuts at: 0 487 511 1686 Size: 487 24 1175 Fragments arranged by size: 1175 487 24 Bme1580I G_kGCm'C Cuts at: 0 19 205 262 1052 1074 1686 Size: 19 186 57 790 22 612 Fragments arranged by size: 790 612 186 57 22 19 BmrI ACTGGGnnnn_n' Cuts at: 0 1489 1686 Size: 1489 197 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 451 517 526 694 724 1024 1039 Size: 202 249 66 9 168 30 300 15 Cuts at: 1039 1415 1430 1441 1639 1654 1664 1686 Size: 376 15 11 198 15 10 22 Fragments arranged by size: 376 300 249 202 198 168 66 30 22 15 15 15 11 10 9 Bpu10I CC'TnA_GC Cuts at: 0 413 569 1686 Size: 413 156 1117 Fragments arranged by size: 1117 413 156 BsaI GGTCTCn'nnnn_ Cuts at: 0 349 1213 1431 1686 Size: 349 864 218 255 Fragments arranged by size: 864 349 255 218 BsaHI Gr'CG_yC Cuts at: 0 522 1686 Size: 522 1164 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 79 112 367 430 586 610 682 808 Size: 79 33 255 63 156 24 72 126 Cuts at: 808 1036 1363 1518 1686 Size: 228 327 155 168 Fragments arranged by size: 327 255 228 168 156 155 126 79 72 63 33 24 BseYI C'CCAG_C Cuts at: 0 75 383 632 1073 1116 1254 1258 1569 Size: 75 308 249 441 43 138 4 311 Cuts at: 1569 1686 Size: 117 Fragments arranged by size: 441 311 308 249 138 117 75 43 4 BsiHKAI G_wGCw'C Cuts at: 0 512 1686 Size: 512 1174 BsmI GAATG_Cn' Cuts at: 0 86 543 1686 Size: 86 457 1143 Fragments arranged by size: 1143 457 86 Bsp1286I G_dGCh'C Cuts at: 0 19 205 262 375 512 1052 1074 1401 Size: 19 186 57 113 137 540 22 327 Cuts at: 1401 1686 Size: 285 Fragments arranged by size: 540 327 285 186 137 113 57 22 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 14 491 1449 1686 Size: 14 477 958 237 Fragments arranged by size: 958 477 237 14 BsrBI CCG'CTC Cuts at: 0 57 819 1686 Size: 57 762 867 Fragments arranged by size: 867 762 57 BsrFI r'CCGG_y Cuts at: 0 84 1686 Size: 84 1602 BstAPI GCAn_nnn'nTGC Cuts at: 0 642 1686 Size: 642 1044 BstBI TT'CG_AA Cuts at: 0 122 1686 Size: 122 1564 BstXI CCAn_nnnn'nTGG Cuts at: 0 240 660 702 1325 1686 Size: 240 420 42 623 361 Fragments arranged by size: 623 420 361 240 42 BstYI r'GATC_y Cuts at: 0 599 1686 Size: 599 1087 Bsu36I CC'TnA_GG Cuts at: 0 11 62 263 1686 Size: 11 51 201 1423 Fragments arranged by size: 1423 201 51 11 BtgI C'CryG_G Cuts at: 0 659 854 1686 Size: 659 195 832 Fragments arranged by size: 832 659 195 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 956 1097 1686 Size: 956 141 589 Fragments arranged by size: 956 589 141 BtsI GCAGTG_nn' Cuts at: 0 32 1204 1686 Size: 32 1172 482 Fragments arranged by size: 1172 482 32 EaeI y'GGCC_r Cuts at: 0 230 692 851 1686 Size: 230 462 159 835 Fragments arranged by size: 835 462 230 159 EarI CTCTTCn'nnn_ Cuts at: 0 344 1077 1332 1340 1686 Size: 344 733 255 8 346 Fragments arranged by size: 733 346 344 255 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 760 1686 Size: 760 926 EcoICRI GAG'CTC Cuts at: 0 510 1686 Size: 510 1176 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 451 517 526 694 703 724 1024 Size: 202 249 66 9 168 9 21 300 Cuts at: 1024 1039 1085 1268 1319 1415 1430 1441 1639 Size: 15 46 183 51 96 15 11 198 Cuts at: 1639 1654 1664 1686 Size: 15 10 22 Fragments arranged by size: 300 249 202 198 183 168 96 66 51 46 22 21 15 15 15 11 10 9 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 10 592 1301 1686 Size: 10 582 709 385 Fragments arranged by size: 709 582 385 10 EcoO109I rG'GnC_Cy Cuts at: 0 15 201 202 258 259 501 639 678 Size: 15 186 1 56 1 242 138 39 Cuts at: 678 1048 1070 1508 1686 Size: 370 22 438 178 Fragments arranged by size: 438 370 242 186 178 138 56 39 22 15 1 1 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 562 591 594 623 768 800 905 937 Size: 562 29 3 29 145 32 105 32 Cuts at: 937 1228 1260 1557 1589 1686 Size: 291 32 297 32 97 Fragments arranged by size: 562 297 291 145 105 97 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 0 325 1331 1686 Size: 325 1006 355 Fragments arranged by size: 1006 355 325 HindIII A'AGCT_T Cuts at: 0 125 1686 Size: 125 1561 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1199 1686 Size: 1199 487 MscI TGG'CCA Cuts at: 0 232 694 853 1686 Size: 232 462 159 833 Fragments arranged by size: 833 462 232 159 MslI CAynn'nnrTG Cuts at: 0 399 1323 1686 Size: 399 924 363 Fragments arranged by size: 924 399 363 MspA1I CmG'CkG Cuts at: 0 478 552 568 1029 1077 1258 1539 1686 Size: 478 74 16 461 48 181 281 147 Fragments arranged by size: 478 461 281 181 147 74 48 16 NcoI C'CATG_G Cuts at: 0 659 854 1686 Size: 659 195 832 Fragments arranged by size: 832 659 195 NruI TCG'CGA Cuts at: 0 941 1686 Size: 941 745 PflMI CCAn_nnn'nTGG Cuts at: 0 171 228 966 1686 Size: 171 57 738 720 Fragments arranged by size: 738 720 171 57 PfoI T'CCnGG_A Cuts at: 0 50 1686 Size: 50 1636 PpuMI rG'GwC_Cy Cuts at: 0 501 678 1686 Size: 501 177 1008 Fragments arranged by size: 1008 501 177 PspOMI G'GGCC_C Cuts at: 0 15 201 258 1048 1070 1686 Size: 15 186 57 790 22 616 Fragments arranged by size: 790 616 186 57 22 15 PstI C_TGCA'G Cuts at: 0 551 647 791 1686 Size: 551 96 144 895 Fragments arranged by size: 895 551 144 96 PvuII CAG'CTG Cuts at: 0 478 568 1029 1077 1258 1686 Size: 478 90 461 48 181 428 Fragments arranged by size: 478 461 428 181 90 48 SacI G_AGCT'C Cuts at: 0 512 1686 Size: 512 1174 SfcI C'TryA_G Cuts at: 0 547 643 787 1686 Size: 547 96 144 899 Fragments arranged by size: 899 547 144 96 StyI C'CwwG_G Cuts at: 0 588 653 659 854 1686 Size: 588 65 6 195 832 Fragments arranged by size: 832 588 195 65 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 13 646 1111 1686 Size: 13 633 465 575 Fragments arranged by size: 633 575 465 13 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1542 1574 1686 Size: 1542 32 112 Fragments arranged by size: 1542 112 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 276 1640 1686 Size: 276 1364 46 Fragments arranged by size: 1364 276 46 ZraI GAC'GTC Cuts at: 0 523 1686 Size: 523 1163 Enzymes that do cut: AatII AcuI AlwNI ApaI AvaI BanI BanII BbsI BbvCI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaHI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI NruI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SfcI StyI TaqII TstI XcmI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI XmaI XmnI