################################## # NO CUT ENZYMES ( 218 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI ItaI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI TseI Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AluI 48 AGCT AG^CT AspHI 204 GTGCTC G[AT]GC[AT]^C AvaII 372 GGACC G^G[AT]CC BanII 3 GGGCTC G[GA]GC[CT]^C BbvCI 44 CCTCAGC CCTCAGC(-5/-2) BciVI 229 GGATAC GTATCC(6/5) BsaJI 185 CCAAGG C^C[ACGT][ACGT]GG BsgI 248 GTGCAG GTGCAG(16/14) BsiHKAI 204 GTGCTC G[AT]GC[AT]^C Cac8I 49 GCTTGC GC[ACGT]^[ACGT]GC Cfr13I 372 GGACC G^G[ACGT]CC DdeI 45 CTCAG C^T[ACGT]AG DpnII 297 GATC ^GATC DraI 69 TTTAAA TTT^AAA EarI 19 CTCTTC CTCTTC(1/4) EcoT14I 185 CCAAGG C^C[AT][AT]GG HgaI 290 GACGC GACGC(5/10) Ksp632I 19 CTCTTC CTCTTC(1/4) MaeIII 118 GTTAC ^GT[ACGT]AC MboI 297 GATC ^GATC MnlI 44 CCTC CCTC(7/6) NdeII 297 GATC ^GATC NlaIV 4 GGCTCC GG[ACGT]^[ACGT]CC Sau3AI 297 GATC ^GATC Sau96I 372 GGACC G^G[ACGT]CC StyI 185 CCAAGG C^C[AT][AT]GG XcmI 147 CCACTATAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### AfaI 285 GTAC GT^AC 311 GTAC BmyI 3 GGGCTC G[AGT]GC[ACT]^C 204 GTGCTC Bsp1286I 3 GGGCTC G[AGT]GC[ACT]^C 204 GTGCTC BsrI 233 ACTGG ACTGG(1/-1) 302 ACTGG RsaI 285 GTAC GT^AC 311 GTAC SfaNI 53 GCATC GCATC(5/9) 176 GCATC TspRI 246 CAGTG CA[GC]TG[ACGT][ACGT]^ 350 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 6 enzymes) # ######################################### AcsI ApoI MboII MseI Tru9I Tsp509I