################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AflIII AgeI Aor51HI AscI AseI AsnI Asp700I AviII AvrII BbeI BbvCI BciVI BclI BfrI BlnI BsaBI BsaHI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII DrdI Eco47III EcoRI EcoRV FbaI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MroI MseI MunI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 1568 GGTACC G^GTACC AcsI 855 GAATTT [GA]^AATT[CT] AhdI 1069 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 397 CCTGAGG CC^T[ACGT]AGG ApoI 855 GAATTT [GA]^AATT[CT] Asp718I 1568 GGTACC G^GTACC AspEI 1069 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1375 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 257 TGGCCA TGG^CCA BbrPI 826 CACGTG CAC^GTG BcgI 288 CGACCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 424 CTAG C^TAG BsaAI 826 CACGTG [CT]AC^GT[GA] BseRI 1555 GAGGAG GAGGAG(10/8) BsiEI 1113 CGGCCG CG[GA][CT]^CG BsmI 37 GAATGC GAATGC(1/-1) BsrFI 65 GCCGGC [GA]^CCGG[CT] BssSI 229 CACGAG CACGAG(-5/-1) BstAPI 218 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1635 GGTCACC G^GT[ACGT]ACC BstPI 1635 GGTCACC G^GT[ACGT]ACC BstXI 134 CCATGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 397 CCTGAGG CC^T[ACGT]AGG Cfr10I 65 GCCGGC [GA]^CCGG[CT] EagI 1113 CGGCCG C^GGCCG Eam1105I 1069 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1113 CGGCCG C^GGCCG Eco52I 1113 CGGCCG C^GGCCG Eco81I 397 CCTGAGG CC^T[ACGT]AGG EcoNI 1080 CCTGCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1635 GGTCACC G^GT[ACGT]ACC EcoT22I 192 ATGCAT ATGCA^T FseI 64 GGCCGGCC GGCCGG^CC KpnI 1568 GGTACC GGTAC^C MaeI 424 CTAG C^TAG MluNI 257 TGGCCA TGG^CCA MscI 257 TGGCCA TGG^CCA NaeI 65 GCCGGC GCC^GGC NcoI 1506 CCATGG C^CATGG NgoMIV 65 GCCGGC G^CCGGC NsiI 192 ATGCAT ATGCA^T PflFI 1375 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 826 CACGTG CAC^GTG PmlI 826 CACGTG CAC^GTG SfiI 450 GGCCACCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 555 AGGCCT AGG^CCT Tth111I 1375 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 1029 CGCG CG^CG 1292 CGCG Alw44I 800 GTGCAC G^TGCAC 1057 GTGCAC ApaI 787 GGGCCC GGGCC^C 1349 GGGCCC ApaLI 800 GTGCAC G^TGCAC 1057 GTGCAC BamHI 414 GGATCC G^GATCC 1540 GGATCC BanI 1568 GGTACC G^G[CT][GA]CC 1901 GGTGCC BbsI 485 GAAGAC GAAGAC(2/6) 1255 GAAGAC BglII 359 AGATCT A^GATCT 1262 AGATCT BlpI 616 GCTCAGC GC^T[ACGT]AGC 774 GCTGAGC Bpu1102I 616 GCTCAGC GC^T[ACGT]AGC 774 GCTGAGC BpuAI 485 GAAGAC GAAGAC(2/6) 1255 GAAGAC BsaI 1748 GAGACC GGTCTC(1/5) 733 GGTCTC BsaWI 43 ACCGGA [AT]^CCGG[AT] 641 TCCGGT BsmBI 348 GAGACG CGTCTC(1/5) 601 GAGACG BstUI 1029 CGCG CG^CG 1292 CGCG CelII 616 GCTCAGC GC^T[ACGT]AGC 774 GCTGAGC DsaI 455 CCGTGG C^C[GA][CT]GG 1506 CCATGG EaeI 257 TGGCCA [CT]^GGCC[GA] 1113 CGGCCG HaeII 1198 AGCGCC [GA]GCGC^[CT] 1585 AGCGCC MaeII 683 ACGT A^CGT 827 ACGT MslI 822 CATTCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 864 CACGCTGATG MvnI 1029 CGCG CG^CG 1292 CGCG SmlI 913 CTCAAG C^T[CT][GA]AG 1063 CTCAAG TaqI 206 TCGA T^CGA 590 TCGA TthHB8I 206 TCGA T^CGA 590 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanII BbvI BcnI BglI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmaI StyI TfiI TseI Tsp45I Tsp509I TspRI XcmI XhoII XmaCI XmaI