(Circular) (Six-base) MAPSORT of: pFN21AE3270.seq Check: 7175 from: 1 to: 6724 pFN21AE3270 6724 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2758 3234 2758 Size: 476 6248 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4534 278 Size: 53 83 186 936 2998 2468 Fragments arranged by size: 2998 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4067 1937 Size: 2130 4594 Acc65I G'GTAC_C Cuts at: 3573 4045 3573 Size: 472 6252 AclI AA'CG_TT Cuts at: 1563 4851 5224 1563 Size: 3288 373 3063 Fragments arranged by size: 3288 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3465 3535 4787 6171 6439 1632 Size: 1833 70 1252 1384 268 1917 Fragments arranged by size: 1917 1833 1384 1252 268 70 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6527 AflIII A'CryG_T Cuts at: 1203 5623 6600 1203 Size: 4420 977 1327 Fragments arranged by size: 4420 1327 977 AgeI A'CCGG_T Cuts at: 5555 5555 Size: 6724 AhdI GACnn_n'nnGTC Cuts at: 3079 5453 3079 Size: 2374 4350 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6724 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6724 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3410 3442 3410 Size: 32 6692 AlwNI CAG_nnn'CTG Cuts at: 2350 2385 2677 2815 3169 4467 6039 2350 Size: 35 292 138 354 1298 1572 3035 Fragments arranged by size: 3035 1572 1298 354 292 138 35 ApaI G_GGCC'C Cuts at: 2796 3358 2796 Size: 562 6162 ApaLI G'TGCA_C Cuts at: 2805 3062 4781 5937 2805 Size: 257 1719 1156 3592 Fragments arranged by size: 3592 1719 1156 257 ApoI r'AATT_y Cuts at: 1493 2860 4033 4259 4411 6520 1493 Size: 1367 1173 226 152 2109 1697 Fragments arranged by size: 2109 1697 1367 1173 226 152 AseI AT'TA_AT Cuts at: 160 1032 5278 160 Size: 872 4246 1606 Fragments arranged by size: 4246 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6724 AvaI C'yCGr_G Cuts at: 1870 1943 2112 2466 2789 3288 3487 4049 1870 Size: 73 169 354 323 499 199 562 4545 Fragments arranged by size: 4545 562 499 354 323 199 169 73 BamHI G'GATC_C Cuts at: 2419 3545 4054 4421 2419 Size: 1126 509 367 4722 Fragments arranged by size: 4722 1126 509 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3573 3906 4045 5500 618 Size: 325 217 627 1786 333 139 1455 1842 Fragments arranged by size: 1842 1786 1455 627 333 325 217 139 BanII G_rGCy'C Cuts at: 1391 2522 2702 2732 2796 2945 2973 2982 3358 Size: 1131 180 30 64 149 28 9 376 Cuts at: 3358 3820 4043 1391 Size: 462 223 4072 Fragments arranged by size: 4072 1131 462 376 223 180 149 64 30 28 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2497 3267 928 Size: 971 598 770 4385 Fragments arranged by size: 4385 971 770 598 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2282 2316 4915 4949 1903 Size: 34 345 34 2599 34 3678 Fragments arranged by size: 3678 2599 345 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4616 5826 4616 Size: 1210 5514 BclI T'GATC_A Cuts at: 1548 1548 Size: 6724 BfrBI ATG'CAT Cuts at: 2199 2199 Size: 6724 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2297 2462 2526 Size: 107 122 71 631 605 625 165 64 Cuts at: 2526 5335 136 Size: 2809 1525 Fragments arranged by size: 2809 1525 631 625 605 165 122 107 71 64 BglII A'GATC_T Cuts at: 2364 3267 6719 2364 Size: 903 3452 2369 Fragments arranged by size: 3452 2369 903 BlpI GC'TnA_GC Cuts at: 2622 2780 4141 2622 Size: 158 1361 5205 Fragments arranged by size: 5205 1361 158 Bme1580I G_kGCm'C Cuts at: 1790 2796 2809 3066 3358 4785 5941 1790 Size: 1006 13 257 292 1427 1156 2573 Fragments arranged by size: 2573 1427 1156 1006 292 257 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6724 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2504 3065 3401 Size: 416 53 83 399 24 1087 561 336 Cuts at: 3401 3912 4455 5413 442 Size: 511 543 958 1753 Fragments arranged by size: 1753 1087 958 561 543 511 416 399 336 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6724 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2494 3502 2494 Size: 1008 5716 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 2660 2744 2896 2987 3911 5384 1239 Size: 971 450 84 152 91 924 1473 2579 Fragments arranged by size: 2579 1473 971 924 450 152 91 84 Bpu10I CC'TnA_GC Cuts at: 2516 2939 2516 Size: 423 6301 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2903 3053 4849 5714 6012 2903 Size: 150 1796 865 298 3615 Fragments arranged by size: 3615 1796 865 298 150 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2744 3747 5387 882 Size: 617 1245 1003 1640 2219 Fragments arranged by size: 2219 1640 1245 1003 617 BsaAI yAC'GTr Cuts at: 493 1204 2833 4515 493 Size: 711 1629 1682 2702 Fragments arranged by size: 2702 1682 1629 711 BsaBI GATnn'nnATC Cuts at: 6718 6718 Size: 6724 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4531 4913 5548 275 Size: 53 83 186 936 2998 382 635 1451 Fragments arranged by size: 2998 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2048 2646 5157 5555 5829 5976 6387 2048 Size: 598 2511 398 274 147 411 2385 Fragments arranged by size: 2511 2385 598 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2633 2663 2829 2859 3410 Size: 30 165 30 1230 30 166 30 551 Cuts at: 3410 3440 3807 3823 3837 3853 1178 Size: 30 367 16 14 16 4049 Fragments arranged by size: 4049 1230 551 367 166 165 30 30 30 30 30 16 16 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3575 1186 Size: 2389 4335 BseYI C'CCAG_C Cuts at: 2105 2274 2424 2760 5927 6423 2105 Size: 169 150 336 3167 496 2406 Fragments arranged by size: 3167 2406 496 336 169 150 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2786 3110 3206 3608 1163 Size: 573 1050 324 96 402 4279 Fragments arranged by size: 4279 1050 573 402 324 96 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3121 4174 4935 5963 1540 Size: 275 186 1120 1053 761 1028 2301 Fragments arranged by size: 2301 1120 1053 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2809 2973 2982 3066 3820 4785 4870 5941 2809 Size: 164 9 84 754 965 85 1071 3592 Fragments arranged by size: 3592 1071 965 754 164 85 84 9 BsmI GAATG_Cn' Cuts at: 2048 4235 4328 2048 Size: 2187 93 4444 Fragments arranged by size: 4444 2187 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2347 2600 2347 Size: 253 6471 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2522 2702 2732 2796 2809 2945 2973 Size: 399 732 180 30 64 13 136 28 Cuts at: 2973 2982 3066 3358 3820 4043 4785 4870 5941 Size: 9 84 292 462 223 742 85 1071 Cuts at: 5941 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 732 462 399 292 223 180 136 85 84 64 30 28 13 9 BspEI T'CCGG_A Cuts at: 6387 6387 Size: 6724 BspHI T'CATG_A Cuts at: 4613 6343 4613 Size: 1730 4994 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2685 2758 3162 3234 3585 4079 844 Size: 1841 73 404 72 351 494 3489 Fragments arranged by size: 3489 1841 494 404 351 73 72 BsrBI CCG'CTC Cuts at: 1394 1823 4611 1394 Size: 429 2788 3507 Fragments arranged by size: 3507 2788 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5219 5393 65 Size: 1183 3971 174 1396 Fragments arranged by size: 3971 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2070 5368 5555 1700 Size: 110 260 3298 187 2869 Fragments arranged by size: 3298 2869 260 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6724 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6724 BssSI C'ACGA_G Cuts at: 2234 4784 5796 2234 Size: 2550 1012 3162 Fragments arranged by size: 3162 2550 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2229 794 Size: 1435 5289 BstBI TT'CG_AA Cuts at: 5528 5528 Size: 6724 BstEII G'GTnAC_C Cuts at: 1184 3640 1184 Size: 2456 4268 BstXI CCAn_nnnn'nTGG Cuts at: 2146 2146 Size: 6724 BstYI r'GATC_y Cuts at: 1972 2364 2419 3214 3267 3545 4054 4421 4806 Size: 392 55 795 53 278 509 367 385 Cuts at: 4806 4823 6275 6361 6373 6719 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 795 509 392 385 367 346 278 86 55 53 17 12 Bsu36I CC'TnA_GG Cuts at: 2403 2403 Size: 6724 BtgI C'CryG_G Cuts at: 513 1065 2460 3511 513 Size: 552 1395 1051 3726 Fragments arranged by size: 3726 1395 1051 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3813 487 Size: 683 56 2587 3398 Fragments arranged by size: 3398 2587 683 56 BtsI GCAGTG_nn' Cuts at: 3682 3745 4240 5034 5054 3682 Size: 63 495 794 20 5352 Fragments arranged by size: 5352 794 495 63 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6724 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6689 DraI TTT'AAA Cuts at: 4028 4028 Size: 6724 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5731 809 Size: 507 4415 1802 Fragments arranged by size: 4415 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2262 3118 4171 Size: 54 1425 50 275 18 432 856 1053 Cuts at: 4171 5060 6689 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1053 889 856 432 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3118 4171 1537 Size: 275 1306 1053 4090 Fragments arranged by size: 4090 1306 1053 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2509 3254 3773 4654 1353 Size: 1156 745 519 881 3423 Fragments arranged by size: 3423 1156 881 745 519 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2278 5311 5685 5831 387 Size: 1064 138 689 3033 374 146 1280 Fragments arranged by size: 3033 1280 1064 689 374 146 138 EcoICRI GAG'CTC Cuts at: 2971 2980 3818 2971 Size: 9 838 5877 Fragments arranged by size: 5877 838 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2660 2744 2896 2987 3465 3535 Size: 393 578 450 84 152 91 478 70 Cuts at: 3535 3911 4787 5384 6171 6439 1239 Size: 376 876 597 787 268 1524 Fragments arranged by size: 1524 876 787 597 578 478 450 393 376 268 152 91 84 70 EcoNI CCTnn'n_nnAGG Cuts at: 3089 3089 Size: 6724 EcoO109I rG'GnC_Cy Cuts at: 2674 2793 2890 2959 3073 3355 3806 2674 Size: 119 97 69 114 282 451 5592 Fragments arranged by size: 5592 451 282 119 114 97 69 EcoRI G'AATT_C Cuts at: 4033 4411 4033 Size: 378 6346 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3676 3676 Size: 6724 FseI GG_CCGG'CC Cuts at: 2074 2074 Size: 6724 FspI TGC'GCA Cuts at: 5230 5230 Size: 6724 HaeII r_GCGC'y Cuts at: 1678 3207 3594 5871 1678 Size: 1529 387 2277 2531 Fragments arranged by size: 2531 2277 1529 387 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2633 2665 3065 3097 3444 Size: 32 135 32 1061 32 400 32 347 Cuts at: 3444 3476 3807 3839 3980 4012 5365 5397 5439 Size: 32 331 32 141 32 1353 32 42 Cuts at: 5439 5471 1373 Size: 32 2626 Fragments arranged by size: 2626 1353 1061 400 347 331 141 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4068 4314 669 Size: 988 281 2130 246 3079 Fragments arranged by size: 3079 2130 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6724 HpaI GTT'AAC Cuts at: 4314 4314 Size: 6724 KpnI G_GTAC'C Cuts at: 3577 4049 3577 Size: 472 6252 MfeI C'AATT_G Cuts at: 4323 4323 Size: 6724 MluI A'CGCG_T Cuts at: 6600 6600 Size: 6724 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5838 6022 1305 Size: 4533 184 2007 Fragments arranged by size: 4533 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2264 10 Size: 54 1425 343 432 4470 Fragments arranged by size: 4470 1425 432 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2831 2873 4682 5041 5200 518 Size: 648 1665 42 1809 359 159 2042 Fragments arranged by size: 2042 1809 1665 648 359 159 42 MspA1I CmG'CkG Cuts at: 1606 2219 2274 2760 2854 3412 3672 3714 3852 Size: 613 55 486 94 558 260 42 138 Cuts at: 3852 4140 4817 5965 6210 1606 Size: 288 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 613 558 486 288 260 245 138 94 55 42 NaeI GCC'GGC Cuts at: 2072 2072 Size: 6724 NcoI C'CATG_G Cuts at: 513 1065 3511 513 Size: 552 2446 3726 Fragments arranged by size: 3726 2446 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6724 NgoMIV G'CCGG_C Cuts at: 2070 2070 Size: 6724 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6724 NotI GC'GGCC_GC Cuts at: 4171 4171 Size: 6724 NsiI A_TGCA'T Cuts at: 2201 2201 Size: 6724 NspI r_CATG'y Cuts at: 1132 1229 2103 2248 3404 4082 5627 1132 Size: 97 874 145 1156 678 1545 2229 Fragments arranged by size: 2229 1545 1156 874 678 145 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6724 PciI A'CATG_T Cuts at: 5623 5623 Size: 6724 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6724 PfoI T'CCnGG_A Cuts at: 2964 2964 Size: 6724 PmeI GTTT'AAAC Cuts at: 4028 4028 Size: 6724 PmlI CAC'GTG Cuts at: 2833 2833 Size: 6724 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3410 3442 3448 3480 4793 4825 6306 6338 3410 Size: 32 6 32 1313 32 1481 32 3796 Fragments arranged by size: 3796 1481 1313 32 32 32 32 6 PpuMI rG'GwC_Cy Cuts at: 2674 3073 3806 2674 Size: 399 733 5592 Fragments arranged by size: 5592 733 399 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6724 PsiI TTA'TAA Cuts at: 4294 4294 Size: 6724 PspOMI G'GGCC_C Cuts at: 2792 3354 2792 Size: 562 6162 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3134 3166 3134 Size: 32 6692 PstI C_TGCA'G Cuts at: 830 2174 2364 2859 3165 3203 3677 3752 4076 Size: 1344 190 495 306 38 474 75 324 Cuts at: 4076 830 Size: 3478 Fragments arranged by size: 3478 1344 495 474 324 306 190 75 38 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6724 PvuII CAG'CTG Cuts at: 2219 2274 2854 3412 3672 3852 2219 Size: 55 580 558 260 180 5091 Fragments arranged by size: 5091 580 558 260 180 55 SacI G_AGCT'C Cuts at: 2973 2982 3820 2973 Size: 9 838 5877 Fragments arranged by size: 5877 838 9 SalI G'TCGA_C Cuts at: 1936 4066 1936 Size: 2130 4594 SbfI CC_TGCA'GG Cuts at: 4076 4076 Size: 6724 ScaI AGT'ACT Cuts at: 4972 6684 4972 Size: 1712 5012 SexAI A'CCwGG_T Cuts at: 2373 3075 3756 2373 Size: 702 681 5341 Fragments arranged by size: 5341 702 681 SfcI C'TryA_G Cuts at: 826 1045 2170 2360 2798 2855 3161 3199 3673 Size: 219 1125 190 438 57 306 38 474 Cuts at: 3673 3748 4072 5207 5888 6079 826 Size: 75 324 1135 681 191 1471 Fragments arranged by size: 1471 1135 1125 681 474 438 324 306 219 191 190 75 57 38 SfiI GGCCn_nnn'nGGCC Cuts at: 2462 2462 Size: 6724 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6724 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6724 SmaI CCC'GGG Cuts at: 1872 2468 2791 3290 4051 1872 Size: 596 323 499 761 4545 Fragments arranged by size: 4545 761 596 499 323 SmlI C'TyrA_G Cuts at: 820 1017 1943 2918 3068 4828 5729 5991 820 Size: 197 926 975 150 1760 901 262 1553 Fragments arranged by size: 1760 1553 975 926 901 262 197 150 SnaBI TAC'GTA Cuts at: 493 4515 493 Size: 4022 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6724 SphI G_CATG'C Cuts at: 1132 4082 1132 Size: 2950 3774 SspI AAT'ATT Cuts at: 5 52 4648 5 Size: 47 4596 2081 Fragments arranged by size: 4596 2081 47 StuI AGG'CCT Cuts at: 1517 2562 1517 Size: 1045 5679 StyI C'CwwG_G Cuts at: 513 1065 2955 3181 3511 3792 4163 513 Size: 552 1890 226 330 281 371 3074 Fragments arranged by size: 3074 1890 552 371 330 281 226 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2266 2357 2752 3385 3537 3894 4751 Size: 1255 686 91 395 633 152 357 857 Cuts at: 4751 4768 4921 5106 5551 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 857 686 633 445 395 357 185 153 152 91 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3052 4970 6682 Size: 274 80 33 51 1443 1075 1918 1712 Cuts at: 6682 96 Size: 138 Fragments arranged by size: 1918 1712 1443 1075 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2829 2861 3823 3855 6684 6716 2829 Size: 32 962 32 2829 32 2837 Fragments arranged by size: 2837 2829 962 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3383 1316 Size: 2067 4657 XbaI T'CTAG_A Cuts at: 4060 4060 Size: 6724 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2962 3610 3851 3951 2962 Size: 648 241 100 5735 Fragments arranged by size: 5735 648 241 100 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6724 XmaI C'CCGG_G Cuts at: 1870 2466 2789 3288 4049 1870 Size: 596 323 499 761 4545 Fragments arranged by size: 4545 761 596 499 323 XmnI GAAnn'nnTTC Cuts at: 4853 4853 Size: 6724 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4532 276 Size: 53 83 186 936 2998 2468 Fragments arranged by size: 2998 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I DraIII EcoRV FspAI KasI NarI NruI PacI PspXI RsrII SacII SanDI SapI SfoI SrfI SwaI