(Linear) (Six-base) MAPSORT of: KIEE3270.seq Check: 355 from: 1 to: 2019 KIEE3270.orf With 182 enzymes: SgfI * December 27, 2011 16:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 753 1229 2019 Size: 753 476 790 Fragments arranged by size: 790 753 476 Acc65I G'GTAC_C Cuts at: 0 1568 2019 Size: 1568 451 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1460 1530 2019 Size: 1460 70 489 Fragments arranged by size: 1460 489 70 AhdI GACnn_n'nnGTC Cuts at: 0 1074 2019 Size: 1074 945 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1405 1437 2019 Size: 1405 32 582 Fragments arranged by size: 1405 582 32 AlwNI CAG_nnn'CTG Cuts at: 0 345 380 672 810 1164 2019 Size: 345 35 292 138 354 855 Fragments arranged by size: 855 354 345 292 138 35 ApaI G_GGCC'C Cuts at: 0 791 1353 2019 Size: 791 562 666 Fragments arranged by size: 791 666 562 ApaLI G'TGCA_C Cuts at: 0 800 1057 2019 Size: 800 257 962 Fragments arranged by size: 962 800 257 ApoI r'AATT_y Cuts at: 0 855 2019 Size: 855 1164 AvaI C'yCGr_G Cuts at: 0 107 461 784 1283 1482 2019 Size: 107 354 323 499 199 537 Fragments arranged by size: 537 499 354 323 199 107 BamHI G'GATC_C Cuts at: 0 414 1540 2019 Size: 414 1126 479 Fragments arranged by size: 1126 479 414 BanI G'GyrC_C Cuts at: 0 1568 1901 2019 Size: 1568 333 118 Fragments arranged by size: 1568 333 118 BanII G_rGCy'C Cuts at: 0 517 697 727 791 940 968 977 1353 Size: 517 180 30 64 149 28 9 376 Cuts at: 1353 1815 2019 Size: 462 204 Fragments arranged by size: 517 462 376 204 180 149 64 30 28 9 BbsI GAAGACnn'nnnn_ Cuts at: 0 492 1262 2019 Size: 492 770 757 Fragments arranged by size: 770 757 492 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 277 311 2019 Size: 277 34 1708 Fragments arranged by size: 1708 277 34 BfrBI ATG'CAT Cuts at: 0 194 2019 Size: 194 1825 BglI GCCn_nnn'nGGC Cuts at: 0 292 457 521 2019 Size: 292 165 64 1498 Fragments arranged by size: 1498 292 165 64 BglII A'GATC_T Cuts at: 0 359 1262 2019 Size: 359 903 757 Fragments arranged by size: 903 757 359 BlpI GC'TnA_GC Cuts at: 0 617 775 2019 Size: 617 158 1244 Fragments arranged by size: 1244 617 158 Bme1580I G_kGCm'C Cuts at: 0 791 804 1061 1353 2019 Size: 791 13 257 292 666 Fragments arranged by size: 791 666 292 257 13 BmrI ACTGGGnnnn_n' Cuts at: 0 499 1060 1396 1907 2019 Size: 499 561 336 511 112 Fragments arranged by size: 561 511 499 336 112 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 489 1497 2019 Size: 489 1008 522 Fragments arranged by size: 1008 522 489 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 655 739 891 982 1906 2019 Size: 205 450 84 152 91 924 113 Fragments arranged by size: 924 450 205 152 113 91 84 Bpu10I CC'TnA_GC Cuts at: 0 511 934 2019 Size: 511 423 1085 Fragments arranged by size: 1085 511 423 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 898 1048 2019 Size: 898 150 971 Fragments arranged by size: 971 898 150 BsaI GGTCTCn'nnnn_ Cuts at: 0 739 1742 2019 Size: 739 1003 277 Fragments arranged by size: 1003 739 277 BsaAI yAC'GTr Cuts at: 0 828 2019 Size: 828 1191 BsaWI w'CCGG_w Cuts at: 0 43 641 2019 Size: 43 598 1378 Fragments arranged by size: 1378 598 43 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 628 658 824 854 1405 1435 1802 1818 Size: 628 30 166 30 551 30 367 16 Cuts at: 1818 1832 1848 2019 Size: 14 16 171 Fragments arranged by size: 628 551 367 171 166 30 30 30 16 16 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1570 2019 Size: 1570 449 BseYI C'CCAG_C Cuts at: 0 100 269 419 755 2019 Size: 100 169 150 336 1264 Fragments arranged by size: 1264 336 169 150 100 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 781 1105 1201 1603 2019 Size: 781 324 96 402 416 Fragments arranged by size: 781 416 402 324 96 BsiEI CG_ry'CG Cuts at: 0 1116 2019 Size: 1116 903 BsiHKAI G_wGCw'C Cuts at: 0 804 968 977 1061 1815 2019 Size: 804 164 9 84 754 204 Fragments arranged by size: 804 754 204 164 84 9 BsmI GAATG_Cn' Cuts at: 0 43 2019 Size: 43 1976 BsmBI CGTCTCn'nnnn_ Cuts at: 0 342 595 2019 Size: 342 253 1424 Fragments arranged by size: 1424 342 253 Bsp1286I G_dGCh'C Cuts at: 0 517 697 727 791 804 940 968 977 Size: 517 180 30 64 13 136 28 9 Cuts at: 977 1061 1353 1815 2019 Size: 84 292 462 204 Fragments arranged by size: 517 462 292 204 180 136 84 64 30 28 13 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 680 753 1157 1229 1580 2019 Size: 680 73 404 72 351 439 Fragments arranged by size: 680 439 404 351 73 72 BsrFI r'CCGG_y Cuts at: 0 65 2019 Size: 65 1954 BssSI C'ACGA_G Cuts at: 0 229 2019 Size: 229 1790 BstAPI GCAn_nnn'nTGC Cuts at: 0 224 2019 Size: 224 1795 BstEII G'GTnAC_C Cuts at: 0 1635 2019 Size: 1635 384 BstXI CCAn_nnnn'nTGG Cuts at: 0 141 2019 Size: 141 1878 BstYI r'GATC_y Cuts at: 0 359 414 1209 1262 1540 2019 Size: 359 55 795 53 278 479 Fragments arranged by size: 795 479 359 278 55 53 Bsu36I CC'TnA_GG Cuts at: 0 398 2019 Size: 398 1621 BtgI C'CryG_G Cuts at: 0 455 1506 2019 Size: 455 1051 513 Fragments arranged by size: 1051 513 455 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1808 2019 Size: 1808 211 BtsI GCAGTG_nn' Cuts at: 0 1677 1740 2019 Size: 1677 63 279 Fragments arranged by size: 1677 279 63 EaeI y'GGCC_r Cuts at: 0 257 1113 2019 Size: 257 856 906 Fragments arranged by size: 906 856 257 EagI C'GGCC_G Cuts at: 0 1113 2019 Size: 1113 906 EarI CTCTTCn'nnn_ Cuts at: 0 504 1249 1768 2019 Size: 504 745 519 251 Fragments arranged by size: 745 519 504 251 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 273 2019 Size: 273 1746 EcoICRI GAG'CTC Cuts at: 0 966 975 1813 2019 Size: 966 9 838 206 Fragments arranged by size: 966 838 206 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 655 739 891 982 1460 1530 1906 Size: 205 450 84 152 91 478 70 376 Cuts at: 1906 2019 Size: 113 Fragments arranged by size: 478 450 376 205 152 113 91 84 70 EcoNI CCTnn'n_nnAGG Cuts at: 0 1084 2019 Size: 1084 935 EcoO109I rG'GnC_Cy Cuts at: 0 669 788 885 954 1068 1350 1801 2019 Size: 669 119 97 69 114 282 451 218 Fragments arranged by size: 669 451 282 218 119 114 97 69 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1671 2019 Size: 1671 348 FseI GG_CCGG'CC Cuts at: 0 69 2019 Size: 69 1950 HaeII r_GCGC'y Cuts at: 0 1202 1589 2019 Size: 1202 387 430 Fragments arranged by size: 1202 430 387 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 628 660 1060 1092 1439 1471 1802 1834 Size: 628 32 400 32 347 32 331 32 Cuts at: 1834 1975 2007 2019 Size: 141 32 12 Fragments arranged by size: 628 400 347 331 141 32 32 32 32 32 12 KpnI G_GTAC'C Cuts at: 0 1572 2019 Size: 1572 447 MscI TGG'CCA Cuts at: 0 259 2019 Size: 259 1760 MslI CAynn'nnrTG Cuts at: 0 826 868 2019 Size: 826 42 1151 Fragments arranged by size: 1151 826 42 MspA1I CmG'CkG Cuts at: 0 214 269 755 849 1407 1667 1709 1847 Size: 214 55 486 94 558 260 42 138 Cuts at: 1847 2019 Size: 172 Fragments arranged by size: 558 486 260 214 172 138 94 55 42 NaeI GCC'GGC Cuts at: 0 67 2019 Size: 67 1952 NcoI C'CATG_G Cuts at: 0 1506 2019 Size: 1506 513 NgoMIV G'CCGG_C Cuts at: 0 65 2019 Size: 65 1954 NsiI A_TGCA'T Cuts at: 0 196 2019 Size: 196 1823 NspI r_CATG'y Cuts at: 0 98 243 1399 2019 Size: 98 145 1156 620 Fragments arranged by size: 1156 620 145 98 PfoI T'CCnGG_A Cuts at: 0 959 2019 Size: 959 1060 PmlI CAC'GTG Cuts at: 0 828 2019 Size: 828 1191 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1405 1437 1443 1475 2019 Size: 1405 32 6 32 544 Fragments arranged by size: 1405 544 32 32 6 PpuMI rG'GwC_Cy Cuts at: 0 669 1068 1801 2019 Size: 669 399 733 218 Fragments arranged by size: 733 669 399 218 PspOMI G'GGCC_C Cuts at: 0 787 1349 2019 Size: 787 562 670 Fragments arranged by size: 787 670 562 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1129 1161 2019 Size: 1129 32 858 Fragments arranged by size: 1129 858 32 PstI C_TGCA'G Cuts at: 0 169 359 854 1160 1198 1672 1747 2019 Size: 169 190 495 306 38 474 75 272 Fragments arranged by size: 495 474 306 272 190 169 75 38 PvuII CAG'CTG Cuts at: 0 214 269 849 1407 1667 1847 2019 Size: 214 55 580 558 260 180 172 Fragments arranged by size: 580 558 260 214 180 172 55 SacI G_AGCT'C Cuts at: 0 968 977 1815 2019 Size: 968 9 838 204 Fragments arranged by size: 968 838 204 9 SexAI A'CCwGG_T Cuts at: 0 368 1070 1751 2019 Size: 368 702 681 268 Fragments arranged by size: 702 681 368 268 SfcI C'TryA_G Cuts at: 0 165 355 793 850 1156 1194 1668 1743 Size: 165 190 438 57 306 38 474 75 Cuts at: 1743 2019 Size: 276 Fragments arranged by size: 474 438 306 276 190 165 75 57 38 SfiI GGCCn_nnn'nGGCC Cuts at: 0 457 2019 Size: 457 1562 SmaI CCC'GGG Cuts at: 0 463 786 1285 2019 Size: 463 323 499 734 Fragments arranged by size: 734 499 463 323 SmlI C'TyrA_G Cuts at: 0 913 1063 2019 Size: 913 150 956 Fragments arranged by size: 956 913 150 StuI AGG'CCT Cuts at: 0 557 2019 Size: 557 1462 StyI C'CwwG_G Cuts at: 0 950 1176 1506 1787 2019 Size: 950 226 330 281 232 Fragments arranged by size: 950 330 281 232 226 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 261 352 747 1380 1532 1889 2019 Size: 261 91 395 633 152 357 130 Fragments arranged by size: 633 395 357 261 152 130 91 TatI w'GTAC_w Cuts at: 0 1047 2019 Size: 1047 972 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 824 856 1818 1850 2019 Size: 824 32 962 32 169 Fragments arranged by size: 962 824 169 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1378 2019 Size: 1378 641 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 957 1605 1846 1946 2019 Size: 957 648 241 100 73 Fragments arranged by size: 957 648 241 100 73 XmaI C'CCGG_G Cuts at: 0 461 784 1283 2019 Size: 461 323 499 736 Fragments arranged by size: 736 499 461 323 Enzymes that do cut: AarI Acc65I AcuI AhdI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BcgI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I KpnI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PfoI PmlI PpiI PpuMI PspOMI PsrI PstI PvuII SacI SexAI SfcI SfiI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AleI AlfI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BciVI BclI BmgBI BmtI BsaBI BsaHI BsiWI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstBI BstZ17I ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FspI FspAI HincII HindIII HpaI KasI MfeI MluI MmeI NarI NdeI NheI NotI NruI PacI PasI PciI PflMI PmeI PshAI PsiI PspXI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmnI ZraI