################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BpuAI BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HapII HgaI HindIII HpaI HpaII KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MslI MspI MunI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 373 GTCGAC GT^[AC][GT]AC AcsI 547 AAATTC [GA]^AATT[CT] AfaI 411 GTAC GT^AC Alw44I 217 GTGCAC G^TGCAC ApaLI 217 GTGCAC G^TGCAC ApoI 547 AAATTC [GA]^AATT[CT] BanII 328 GAGCTC G[GA]GC[CT]^C BglI 47 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 42 GCTCAGC GC^T[ACGT]AGC Bpu1102I 42 GCTCAGC GC^T[ACGT]AGC BsaAI 412 TACGTG [CT]AC^GT[GA] BsiEI 404 CGGCCG CG[GA][CT]^CG BsrBI 115 GAGCGG CCGCTC(-3/-3) BsrI 500 CCAGT ACTGG(1/-1) BssSI 100 CACGAG CACGAG(-5/-1) CelII 42 GCTCAGC GC^T[ACGT]AGC DdeI 43 CTCAG C^T[ACGT]AG DraIII 582 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 382 GACACGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 170 CCATGG C^C[GA][CT]GG EagI 404 CGGCCG C^GGCCG EarI 325 GAAGAG CTCTTC(1/4) EclXI 404 CGGCCG C^GGCCG Eco52I 404 CGGCCG C^GGCCG EcoT14I 170 CCATGG C^C[AT][AT]GG HincII 373 GTCGAC GT[CT]^[GA]AC HindII 373 GTCGAC GT[CT]^[GA]AC HinfI 570 GAGTC G^A[ACGT]TC Ksp632I 325 GAAGAG CTCTTC(1/4) MaeII 413 ACGT A^CGT MaeIII 391 GTCAC ^GT[ACGT]AC MboII 325 GAAGA GAAGA(8/7) NcoI 170 CCATGG C^CATGG PleI 570 GAGTC GAGTC(4/5) PshAI 280 GACACCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 238 CTGCAG CTGCA^G RsaI 411 GTAC GT^AC SacI 328 GAGCTC GAGCT^C SalI 373 GTCGAC G^TCGAC SapI 325 GAAGAGC GCTCTTC(1/4) SfaNI 485 GCATC GCATC(5/9) SfcI 238 CTGCAG C^T[GA][CT]AG SmlI 331 CTCAAG C^T[CT][GA]AG StyI 170 CCATGG C^C[AT][AT]GG TaqI 374 TCGA T^CGA Tsp45I 391 GTCAC ^GT[GC]AC TspRI 501 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 374 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 55 GGCGCC G[GA]^CG[CT]C 95 GGCGCC AlwI 162 GATCC GGATC(4/5) 348 GGATC AlwNI 34 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG 241 CAGCAGCTG AspHI 217 GTGCAC G[AT]GC[AT]^C 328 GAGCTC BbeI 55 GGCGCC GGCGC^C 95 GGCGCC BmyI 217 GTGCAC G[AGT]GC[ACT]^C 328 GAGCTC BsaHI 55 GGCGCC G[GA]^CG[CT]C 95 GGCGCC BseRI 22 GAGGAG GAGGAG(10/8) 274 GAGGAG BsiHKAI 217 GTGCAC G[AT]GC[AT]^C 328 GAGCTC Bsp1286I 217 GTGCAC G[AGT]GC[ACT]^C 328 GAGCTC BspMI 105 GCAGGT ACCTGC(4/8) 213 GCAGGT BstXI 314 CCAGAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 515 CCATCCACCTGG BstYI 161 AGATCC [GA]^GATC[CT] 348 GGATCT Cac8I 47 GCCTGC GC[ACGT]^[ACGT]GC 295 GCCAGC DpnII 162 GATC ^GATC 349 GATC EaeI 2 TGGCCG [CT]^GGCC[GA] 404 CGGCCG HaeII 55 GGCGCC [GA]GCGC^[CT] 95 GGCGCC Hin1I 55 GGCGCC G[GA]^CG[CT]C 95 GGCGCC KasI 55 GGCGCC G^GCGCC 95 GGCGCC MboI 162 GATC ^GATC 349 GATC MflI 161 AGATCC [GA]^GATC[CT] 348 GGATCT MspA1I 151 CAGCTG C[AC]G^C[GT]G 244 CAGCTG NarI 55 GGCGCC GG^CGCC 95 GGCGCC NdeII 162 GATC ^GATC 349 GATC NlaIII 171 CATG CATG^ 493 CATG PvuII 151 CAGCTG CAG^CTG 244 CAGCTG Sau3AI 162 GATC ^GATC 349 GATC SfoI 55 GGCGCC GGC^GCC 95 GGCGCC Tsp509I 444 AATT ^AATT 548 AATT XhoII 161 AGATCC [GA]^GATC[CT] 348 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AccII AciI AluI BanI BbvI BpmI BsaJI BsiYI BslI BssKI BstF5I BstNI BstUI CfoI Cfr13I EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HphI ItaI MnlI MvaI MvnI MwoI NlaIV Sau96I ScrFI TseI