(Circular) (Six-base) MAPSORT of: pFN21AE9602.seq Check: 392 from: 1 to: 5296 pFN21AE9602 5296 bp With 182 enzymes: SgfI * March 28, 2012 12:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2101 2209 2101 Size: 108 5188 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3106 278 Size: 53 83 186 936 1570 2468 Fragments arranged by size: 2468 1570 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2379 2639 1937 Size: 442 260 4594 Fragments arranged by size: 4594 442 260 Acc65I G'GTAC_C Cuts at: 2617 2617 Size: 5296 AclI AA'CG_TT Cuts at: 1563 3423 3796 1563 Size: 1860 373 3063 Fragments arranged by size: 3063 1860 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2249 2294 2315 3359 4743 5011 1632 Size: 617 45 21 1044 1384 268 1917 Fragments arranged by size: 1917 1384 1044 617 268 45 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5099 AflIII A'CryG_T Cuts at: 1203 4195 5172 1203 Size: 2992 977 1327 Fragments arranged by size: 2992 1327 977 AgeI A'CCGG_T Cuts at: 4127 4127 Size: 5296 AhdI GACnn_n'nnGTC Cuts at: 4025 4025 Size: 5296 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5296 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2160 1184 Size: 976 4320 AlwNI CAG_nnn'CTG Cuts at: 2044 2251 3039 4611 2044 Size: 207 788 1572 2729 Fragments arranged by size: 2729 1572 788 207 ApaLI G'TGCA_C Cuts at: 2222 3353 4509 2222 Size: 1131 1156 3009 Fragments arranged by size: 3009 1156 1131 ApoI r'AATT_y Cuts at: 1493 2552 2605 2831 2983 5092 1493 Size: 1059 53 226 152 2109 1697 Fragments arranged by size: 2109 1697 1059 226 152 53 AseI AT'TA_AT Cuts at: 160 1032 3850 160 Size: 872 2818 1606 Fragments arranged by size: 2818 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5296 AvaI C'yCGr_G Cuts at: 1870 1943 2621 1870 Size: 73 678 4545 Fragments arranged by size: 4545 678 73 BamHI G'GATC_C Cuts at: 2626 2993 2626 Size: 367 4929 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2100 2144 2617 4072 Size: 325 217 627 273 40 44 473 1455 Cuts at: 4072 618 Size: 1842 Fragments arranged by size: 1842 1455 627 473 325 273 217 44 40 BanII G_rGCy'C Cuts at: 1391 2337 2615 1391 Size: 946 278 4072 Fragments arranged by size: 4072 946 278 BbeI G_GCGC'C Cuts at: 2064 2104 2064 Size: 40 5256 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4325 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3487 3521 1903 Size: 34 1550 34 3678 Fragments arranged by size: 3678 1550 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3188 4398 3188 Size: 1210 4086 BclI T'GATC_A Cuts at: 1548 1548 Size: 5296 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2058 3907 136 Size: 107 122 71 631 605 386 1849 1525 Fragments arranged by size: 1849 1525 631 605 386 122 107 71 BglII A'GATC_T Cuts at: 5291 5291 Size: 5296 BlpI GC'TnA_GC Cuts at: 2048 2713 2048 Size: 665 4631 Bme1580I G_kGCm'C Cuts at: 1790 2226 3357 4513 1790 Size: 436 1131 1156 2573 Fragments arranged by size: 2573 1156 1131 436 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5296 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2499 3027 3985 Size: 416 53 83 399 24 1082 528 958 Cuts at: 3985 442 Size: 1753 Fragments arranged by size: 1753 1082 958 528 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5296 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2186 2186 Size: 5296 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2378 2530 2593 3956 1239 Size: 1139 152 63 1363 2579 Fragments arranged by size: 2579 1363 1139 152 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2321 3421 4286 4584 2321 Size: 1100 865 298 3033 Fragments arranged by size: 3033 1100 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3959 882 Size: 617 2460 2219 Fragments arranged by size: 2460 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2419 3087 493 Size: 711 1215 668 2702 Fragments arranged by size: 2702 1215 711 668 BsaBI GATnn'nnATC Cuts at: 5290 5290 Size: 5296 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2061 2101 3103 3485 Size: 53 83 186 936 528 40 1002 382 Cuts at: 3485 4120 275 Size: 635 1451 Fragments arranged by size: 1451 1002 936 635 528 382 186 83 53 40 BsaWI w'CCGG_w Cuts at: 3729 4127 4401 4548 4959 3729 Size: 398 274 147 411 4066 Fragments arranged by size: 4066 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5071 Fragments arranged by size: 5071 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2294 1186 Size: 856 252 4188 Fragments arranged by size: 4188 856 252 BseYI C'CCAG_C Cuts at: 2056 2095 2258 2273 2514 2538 4499 4995 2056 Size: 39 163 15 241 24 1961 496 2357 Fragments arranged by size: 2357 1961 496 241 163 39 24 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4723 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2412 2746 3507 4535 1540 Size: 275 186 411 334 761 1028 2301 Fragments arranged by size: 2301 1028 761 411 334 275 186 BsiHKAI G_wGCw'C Cuts at: 2226 2337 3357 3442 4513 2226 Size: 111 1020 85 1071 3009 Fragments arranged by size: 3009 1071 1020 111 85 BsmI GAATG_Cn' Cuts at: 2807 2900 2807 Size: 93 5203 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2337 2615 3357 3442 4513 1391 Size: 399 436 111 278 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 436 399 278 111 85 BspEI T'CCGG_A Cuts at: 4959 4959 Size: 5296 BspHI T'CATG_A Cuts at: 3185 4915 3185 Size: 1730 3566 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2101 2209 2651 844 Size: 1257 108 442 3489 Fragments arranged by size: 3489 1257 442 108 BsrBI CCG'CTC Cuts at: 1394 1823 2122 3183 1394 Size: 429 299 1061 3507 Fragments arranged by size: 3507 1061 429 299 BsrDI GCAATG_nn' Cuts at: 65 1248 3791 3965 65 Size: 1183 2543 174 1396 Fragments arranged by size: 2543 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3940 4127 1700 Size: 110 2130 187 2869 Fragments arranged by size: 2869 2130 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5296 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5296 BssSI C'ACGA_G Cuts at: 2105 3356 4368 2105 Size: 1251 1012 3033 Fragments arranged by size: 3033 1251 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5296 BstBI TT'CG_AA Cuts at: 4100 4100 Size: 5296 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5296 BstXI CCAn_nnnn'nTGG Cuts at: 2326 2527 2326 Size: 201 5095 BstYI r'GATC_y Cuts at: 1972 2166 2353 2626 2993 3378 3395 4847 4933 Size: 194 187 273 367 385 17 1452 86 Cuts at: 4933 4945 5291 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 273 194 187 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2175 513 Size: 552 939 171 3634 Fragments arranged by size: 3634 939 552 171 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4557 Fragments arranged by size: 4557 683 56 BtsI GCAGTG_nn' Cuts at: 2812 3606 3626 2812 Size: 794 20 4482 Fragments arranged by size: 4482 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5296 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5261 DraI TTT'AAA Cuts at: 2600 2600 Size: 5296 DraIII CAC_nnn'GTG Cuts at: 2592 2592 Size: 5296 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2393 4303 809 Size: 507 1077 1910 1802 Fragments arranged by size: 1910 1802 1077 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2409 2743 Size: 54 1425 50 275 18 177 402 334 Cuts at: 2743 3632 5261 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 402 334 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2409 2743 1537 Size: 275 597 334 4090 Fragments arranged by size: 4090 597 334 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2325 3226 1353 Size: 972 901 3423 Fragments arranged by size: 3423 972 901 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2366 3883 4257 4403 387 Size: 1064 138 777 1517 374 146 1280 Fragments arranged by size: 1517 1280 1064 777 374 146 138 EcoICRI GAG'CTC Cuts at: 2335 2335 Size: 5296 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2294 2315 2378 2530 2593 3359 Size: 393 617 45 21 63 152 63 766 Cuts at: 3359 3956 4743 5011 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 766 617 597 393 268 152 63 63 45 21 EcoRI G'AATT_C Cuts at: 2605 2983 2605 Size: 378 4918 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2279 2279 Size: 5296 FspI TGC'GCA Cuts at: 3802 3802 Size: 5296 HaeII r_GCGC'y Cuts at: 1678 2064 2104 4443 1678 Size: 386 40 2339 2531 Fragments arranged by size: 2531 2339 386 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3937 3969 4011 4043 1373 Size: 32 135 32 2365 32 42 32 2626 Fragments arranged by size: 2626 2365 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2380 2640 2886 669 Size: 988 281 442 260 246 3079 Fragments arranged by size: 3079 988 442 281 260 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5296 HpaI GTT'AAC Cuts at: 2886 2886 Size: 5296 KasI G'GCGC_C Cuts at: 2060 2100 2060 Size: 40 5256 KpnI G_GTAC'C Cuts at: 2621 2621 Size: 5296 MfeI C'AATT_G Cuts at: 2895 2895 Size: 5296 MluI A'CGCG_T Cuts at: 5172 5172 Size: 5296 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4410 4594 1305 Size: 3105 184 2007 Fragments arranged by size: 3105 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3474 Fragments arranged by size: 3474 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3254 3613 3772 518 Size: 648 2088 359 159 2042 Fragments arranged by size: 2088 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2158 2251 2712 3389 4537 4782 1606 Size: 552 93 461 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 552 461 245 93 NarI GG'CG_CC Cuts at: 2061 2101 2061 Size: 40 5256 NcoI C'CATG_G Cuts at: 513 1065 2004 2175 513 Size: 552 939 171 3634 Fragments arranged by size: 3634 939 552 171 NdeI CA'TA_TG Cuts at: 387 387 Size: 5296 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5296 NotI GC'GGCC_GC Cuts at: 2743 2743 Size: 5296 NspI r_CATG'y Cuts at: 1132 1229 2654 4199 1132 Size: 97 1425 1545 2229 Fragments arranged by size: 2229 1545 1425 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5296 PciI A'CATG_T Cuts at: 4195 4195 Size: 5296 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5296 PfoI T'CCnGG_A Cuts at: 2546 2546 Size: 5296 PmeI GTTT'AAAC Cuts at: 2600 2600 Size: 5296 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3365 3397 4878 4910 3365 Size: 32 1481 32 3751 Fragments arranged by size: 3751 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2289 1613 Size: 676 4620 PsiI TTA'TAA Cuts at: 2866 2866 Size: 5296 PstI C_TGCA'G Cuts at: 830 2247 2648 830 Size: 1417 401 3478 Fragments arranged by size: 3478 1417 401 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5296 PvuII CAG'CTG Cuts at: 2158 2251 2158 Size: 93 5203 SacI G_AGCT'C Cuts at: 2337 2337 Size: 5296 SalI G'TCGA_C Cuts at: 1936 2378 2638 1936 Size: 442 260 4594 Fragments arranged by size: 4594 442 260 SapI GCTCTTCn'nnn_ Cuts at: 2325 2325 Size: 5296 SbfI CC_TGCA'GG Cuts at: 2648 2648 Size: 5296 ScaI AGT'ACT Cuts at: 3544 5256 3544 Size: 1712 3584 SfcI C'TryA_G Cuts at: 826 1045 2243 2644 3779 4460 4651 826 Size: 219 1198 401 1135 681 191 1471 Fragments arranged by size: 1471 1198 1135 681 401 219 191 SfoI GGC'GCC Cuts at: 2062 2102 2062 Size: 40 5256 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5296 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5296 SmaI CCC'GGG Cuts at: 1872 2623 1872 Size: 751 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2336 3400 4301 4563 820 Size: 197 926 393 1064 901 262 1553 Fragments arranged by size: 1553 1064 926 901 393 262 197 SnaBI TAC'GTA Cuts at: 493 3087 493 Size: 2594 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5296 SphI G_CATG'C Cuts at: 1132 2654 1132 Size: 1522 3774 SspI AAT'ATT Cuts at: 5 52 3220 5 Size: 47 3168 2081 Fragments arranged by size: 3168 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5296 StyI C'CwwG_G Cuts at: 513 1065 2004 2175 2735 513 Size: 552 939 171 560 3074 Fragments arranged by size: 3074 939 560 552 171 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2552 3323 3340 3493 3678 4123 325 Size: 1255 972 771 17 153 185 445 1498 Fragments arranged by size: 1498 1255 972 771 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3542 5254 96 Size: 274 80 33 51 1443 1565 1712 138 Fragments arranged by size: 1712 1565 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2450 2482 2576 2608 5256 5288 2450 Size: 32 94 32 2648 32 2458 Fragments arranged by size: 2648 2458 94 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5296 XbaI T'CTAG_A Cuts at: 2632 2632 Size: 5296 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5296 XmaI C'CCGG_G Cuts at: 1870 2621 1870 Size: 751 4545 XmnI GAAnn'nnTTC Cuts at: 3425 3425 Size: 5296 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3104 276 Size: 53 83 186 936 1570 2468 Fragments arranged by size: 2468 1570 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI