################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AccI AccIII AclI AgeI Aor51HI AscI AseI AsnI AviII AvrII BalI BcgI BclI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI Bst1107I BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII Eco47III EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KspI MamI MfeI MluNI MroI MscI MunI NcoI NdeI NheI NotI NruI NsiI PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI ScaI SexAI SfiI SnaBI SpeI SphI SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AflII 2383 CTTAAG C^TTAAG Asp700I 382 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 81 GGATCC G^GATCC BbeI 1300 GGCGCC GGCGC^C BbrPI 733 CACGTG CAC^GTG BbsI 1081 GTCTTC GAAGAC(2/6) BciVI 479 GTATCC GTATCC(6/5) BfaI 352 CTAG C^TAG BfrI 2383 CTTAAG C^TTAAG BglI 2260 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1081 GTCTTC GAAGAC(2/6) BsaAI 733 CACGTG [CT]AC^GT[GA] BsiEI 540 CGGCCG CG[GA][CT]^CG BspLU11I 1349 ACATGT A^CATGT BssSI 684 CTCGTG CACGAG(-5/-1) BstBI 49 TTCGAA TT^CGAA BstYI 81 GGATCC [GA]^GATC[CT] EagI 540 CGGCCG C^GGCCG EclXI 540 CGGCCG C^GGCCG Eco52I 540 CGGCCG C^GGCCG EcoRI 2313 GAATTC G^AATTC KasI 1300 GGCGCC G^GCGCC MaeI 352 CTAG C^TAG MflI 81 GGATCC [GA]^GATC[CT] MluI 695 ACGCGT A^CGCGT MslI 798 CATTGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 1300 GGCGCC GG^CGCC NspI 1349 ACATGT [GA]CATG^[CT] NspV 49 TTCGAA TT^CGAA PmaCI 733 CACGTG CAC^GTG PmlI 733 CACGTG CAC^GTG PshAI 1750 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 72 CCTGCAGG CCTGCA^GG SfoI 1300 GGCGCC GGC^GCC SfuI 49 TTCGAA TT^CGAA SgrAI 1123 CACCGGCG C[GA]^CCGG[CT]G Sse8387I 72 CCTGCAGG CCTGCA^GG XcmI 2255 CCAAAGCCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 81 GGATCC [GA]^GATC[CT] XmnI 382 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AatII 850 GACGTC GACGT^C 2190 GACGTC Acc65I 268 GGTACC G^GTACC 1025 GGTACC AflIII 695 ACGCGT A^C[GA][CT]GT 1349 ACATGT AhdI 922 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1909 GACTCCAGGTC Alw44I 805 GTGCAC G^TGCAC 1135 GTGCAC AocI 1244 CCTCAGG CC^T[ACGT]AGG 1600 CCTGAGG ApaLI 805 GTGCAC G^TGCAC 1135 GTGCAC Asp718I 268 GGTACC G^GTACC 1025 GGTACC AspEI 922 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1909 GACTCCAGGTC AspI 361 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1414 GACTCTGTC BbvCI 291 CCTCAGC CCTCAGC(-5/-2) 936 GCTGAGG BsrBI 1356 CCGCTC CCGCTC(-3/-3) 1464 CCGCTC BstAPI 135 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 906 GCAGGCGCTGC Bsu36I 1244 CCTCAGG CC^T[ACGT]AGG 1600 CCTGAGG DrdI 1033 GACGCACTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1942 GACCAAGAAGTC DsaI 1618 CCGTGG C^C[GA][CT]GG 2290 CCACGG Eam1105I 922 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1909 GACTCCAGGTC Eco81I 1244 CCTCAGG CC^T[ACGT]AGG 1600 CCTGAGG EcoNI 72 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 351 CCTAGCCCAGG EcoT14I 304 CCTTGG C^C[AT][AT]GG 881 CCTTGG KpnI 268 GGTACC GGTAC^C 1025 GGTACC MseI 896 TTAA T^TAA 2384 TTAA NaeI 229 GCCGGC GCC^GGC 2363 GCCGGC NgoMIV 229 GCCGGC G^CCGGC 2363 GCCGGC PflFI 361 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1414 GACTCTGTC SapI 1014 GCTCTTC GCTCTTC(1/4) 1898 GCTCTTC SmlI 439 CTTGAG C^T[CT][GA]AG 2383 CTTAAG StuI 425 AGGCCT AGG^CCT 1115 AGGCCT StyI 304 CCTTGG C^C[AT][AT]GG 881 CCTTGG Tru9I 896 TTAA T^TAA 2384 TTAA Tth111I 361 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1414 GACTCTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XmaCI XmaI