Restriction map for
pFN21AE4226
(Circular) (Six-base) MAPSORT of: pFN21AE4226.seq Check: 5146 from: 1 to: 7108
pFN21AE4226 7108 bp
With 182 enzymes: SgfI *
March 28, 2012 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2313 2775 3153 2313
Size: 462 378 6268
Fragments arranged by size:
6268 462 378
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2859 4199 4918 278
Size: 53 83 186 936 1323 1340 719 2468
Fragments arranged by size:
2468 1340 1323 936 719 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4451 1937
Size: 2514 4594
Acc65I G'GTAC_C
Cuts at: 2273 3030 4429 2273
Size: 757 1399 4952
Fragments arranged by size:
4952 1399 757
AclI AA'CG_TT
Cuts at: 1563 5235 5608 1563
Size: 3672 373 3063
Fragments arranged by size:
3672 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2040 2104 3058 3772 4095 5171 6555 6823
Size: 408 64 954 714 323 1076 1384 268
Cuts at: 6823 1632
Size: 1917
Fragments arranged by size:
1917 1384 1076 954 714 408 323 268
64
AflII C'TTAA_G
Cuts at: 820 1017 4388 820
Size: 197 3371 3540
Fragments arranged by size:
3540 3371 197
AflIII A'CryG_T
Cuts at: 1203 2700 3354 6007 6984 1203
Size: 1497 654 2653 977 1327
Fragments arranged by size:
2653 1497 1327 977 654
AgeI A'CCGG_T
Cuts at: 5939 5939
Size: 7108
AhdI GACnn_n'nnGTC
Cuts at: 2932 3919 5837 2932
Size: 987 1918 4203
Fragments arranged by size:
4203 1918 987
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3064 1184
Size: 1880 5228
AlwNI CAG_nnn'CTG
Cuts at: 2416 2917 3016 3986 4344 4851 6423 2416
Size: 501 99 970 358 507 1572 3101
Fragments arranged by size:
3101 1572 970 507 501 358 99
ApaI G_GGCC'C
Cuts at: 2427 3259 3454 2427
Size: 832 195 6081
Fragments arranged by size:
6081 832 195
ApaLI G'TGCA_C
Cuts at: 2810 3140 5165 6321 2810
Size: 330 2025 1156 3597
Fragments arranged by size:
3597 2025 1156 330
ApoI r'AATT_y
Cuts at: 1493 2153 4155 4318 4417 4643 4795 6904 1493
Size: 660 2002 163 99 226 152 2109 1697
Fragments arranged by size:
2109 2002 1697 660 226 163 152 99
AseI AT'TA_AT
Cuts at: 160 1032 5662 160
Size: 872 4630 1606
Fragments arranged by size:
4630 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7108
AvaI C'yCGr_G
Cuts at: 1870 1943 2499 2975 3055 4433 1870
Size: 73 556 476 80 1378 4545
Fragments arranged by size:
4545 1378 556 476 80 73
BamHI G'GATC_C
Cuts at: 2086 4438 4805 2086
Size: 2352 367 4389
Fragments arranged by size:
4389 2352 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2146 2273 3030 3109 3305
Size: 325 217 627 359 127 757 79 196
Cuts at: 3305 3618 4429 5884 618
Size: 313 811 1455 1842
Fragments arranged by size:
1842 1455 811 757 627 359 325 313
217 196 127 79
BanII G_rGCy'C
Cuts at: 1391 2030 2187 2427 3259 3443 3454 3576 3605
Size: 639 157 240 832 184 11 122 29
Cuts at: 3605 3849 4038 4134 4252 4315 4427 1391
Size: 244 189 96 118 63 112 4072
Fragments arranged by size:
4072 832 639 244 240 189 184 157
122 118 112 96 63 29 11
BbeI G_GCGC'C
Cuts at: 3309 3309
Size: 7108
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3079 928
Size: 971 1180 4957
Fragments arranged by size:
4957 1180 971
BbvCI CC'TCA_GC
Cuts at: 2297 2942 2297
Size: 645 6463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5299 5333 1903
Size: 34 3362 34 3678
Fragments arranged by size:
3678 3362 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2495 5000 6210 2495
Size: 2505 1210 3393
Fragments arranged by size:
3393 2505 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7108
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4271 5719 136
Size: 107 122 71 631 605 2599 1448 1525
Fragments arranged by size:
2599 1525 1448 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7103 7103
Size: 7108
BlpI GC'TnA_GC
Cuts at: 4525 4525
Size: 7108
Bme1580I G_kGCm'C
Cuts at: 1790 2427 2814 3112 3144 3259 3454 3623 5169
Size: 637 387 298 32 115 195 169 1546
Cuts at: 5169 6325 1790
Size: 1156 2573
Fragments arranged by size:
2573 1546 1156 637 387 298 195 169
115 32
BmgBI CAC'GTC
Cuts at: 1318 2908 2929 3960 1318
Size: 1590 21 1031 4466
Fragments arranged by size:
4466 1590 1031 21
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3964 4221 4839
Size: 416 53 83 399 24 2547 257 618
Cuts at: 4839 5797 442
Size: 958 1753
Fragments arranged by size:
2547 1753 958 618 416 399 257 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7108
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2675 3223 2675
Size: 548 6560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2097 2455 2870 2907 3200 3684 3901 4125
Size: 858 358 415 37 293 484 217 224
Cuts at: 4125 4256 4274 5768 1239
Size: 131 18 1494 2579
Fragments arranged by size:
2579 1494 858 484 415 358 293 224
217 131 37 18
Bpu10I CC'TnA_GC
Cuts at: 2297 2942 2954 2297
Size: 645 12 6451
Fragments arranged by size:
6451 645 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2465 5233 6098 6396 2465
Size: 2768 865 298 3177
Fragments arranged by size:
3177 2768 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2343 2473 3730 3765 5771 882
Size: 617 844 130 1257 35 2006 2219
Fragments arranged by size:
2219 2006 1257 844 617 130 35
BsaAI yAC'GTr
Cuts at: 493 1204 2740 4899 493
Size: 711 1536 2159 2702
Fragments arranged by size:
2702 2159 1536 711
BsaBI GATnn'nnATC
Cuts at: 7102 7102
Size: 7108
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2856 2877 3306 3547
Size: 53 83 186 936 1323 21 429 241
Cuts at: 3547 4196 4915 5297 5932 275
Size: 649 719 382 635 1451
Fragments arranged by size:
1451 1323 936 719 649 635 429 382
241 186 83 53 21
BsaWI w'CCGG_w
Cuts at: 5541 5939 6213 6360 6771 5541
Size: 398 274 147 411 5878
Fragments arranged by size:
5878 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2451 2481 1178
Size: 30 165 30 1048 30 5805
Fragments arranged by size:
5805 1048 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3238 3783 4014 1186
Size: 2052 545 231 4280
Fragments arranged by size:
4280 2052 545 231
BseYI C'CCAG_C
Cuts at: 2189 2449 2730 3454 3464 3536 3849 3924 4133
Size: 260 281 724 10 72 313 75 209
Cuts at: 4133 4200 4356 4361 6311 6807 2189
Size: 67 156 5 1950 496 2490
Fragments arranged by size:
2490 1950 724 496 313 281 260 209
156 75 72 67 10 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2222 2930 3299 3410 4104 1163
Size: 573 486 708 369 111 694 4167
Fragments arranged by size:
4167 708 694 573 486 369 111
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2548 4558 5319 6347 1540
Size: 275 186 547 2010 761 1028 2301
Fragments arranged by size:
2301 2010 1028 761 547 275 186
BsiHKAI G_wGCw'C
Cuts at: 2739 2814 3144 5169 5254 6325 2739
Size: 75 330 2025 85 1071 3522
Fragments arranged by size:
3522 2025 1071 330 85 75
BsmI GAATG_Cn'
Cuts at: 4619 4712 4619
Size: 93 7015
BsmBI CGTCTCn'nnnn_
Cuts at: 2885 2999 3538 3966 2885
Size: 114 539 428 6027
Fragments arranged by size:
6027 539 428 114
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2187 2427 2739 2814 3112 3144
Size: 399 240 157 240 312 75 298 32
Cuts at: 3144 3259 3443 3454 3576 3605 3623 3849 4038
Size: 115 184 11 122 29 18 226 189
Cuts at: 4038 4134 4252 4315 4427 5169 5254 6325 1391
Size: 96 118 63 112 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 312 298 240 240
226 189 184 157 122 118 115 112
96 85 75 63 32 29 18 11
BspEI T'CCGG_A
Cuts at: 6771 6771
Size: 7108
BspHI T'CATG_A
Cuts at: 4997 6727 4997
Size: 1730 5378
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2313 2775 3153 4463 844
Size: 1469 462 378 1310 3489
Fragments arranged by size:
3489 1469 1310 462 378
BsrBI CCG'CTC
Cuts at: 1394 1823 3363 3471 4995 1394
Size: 429 1540 108 1524 3507
Fragments arranged by size:
3507 1540 1524 429 108
BsrDI GCAATG_nn'
Cuts at: 65 1248 5603 5777 65
Size: 1183 4355 174 1396
Fragments arranged by size:
4355 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2234 3129 3522 4368 5752 5939 1700
Size: 110 424 895 393 846 1384 187 2869
Fragments arranged by size:
2869 1384 895 846 424 393 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7108
BssHII G'CGCG_C
Cuts at: 1925 2709 2784 4279 1925
Size: 784 75 1495 4754
Fragments arranged by size:
4754 1495 784 75
BssSI C'ACGA_G
Cuts at: 2689 5168 6180 2689
Size: 2479 1012 3617
Fragments arranged by size:
3617 2479 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2146 2917 794
Size: 1352 771 4985
Fragments arranged by size:
4985 1352 771
BstBI TT'CG_AA
Cuts at: 2055 5912 2055
Size: 3857 3251
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7108
BstXI CCAn_nnnn'nTGG
Cuts at: 2887 3104 4002 4234 2887
Size: 217 898 232 5761
Fragments arranged by size:
5761 898 232 217
BstYI r'GATC_y
Cuts at: 1972 2086 4438 4805 5190 5207 6659 6745 6757
Size: 114 2352 367 385 17 1452 86 12
Cuts at: 6757 7103 1972
Size: 346 1977
Fragments arranged by size:
2352 1977 1452 385 367 346 114 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3250 3606 3250
Size: 356 6752
BtgI C'CryG_G
Cuts at: 513 1065 2004 3623 4295 513
Size: 552 939 1619 672 3326
Fragments arranged by size:
3326 1619 939 672 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6369
Fragments arranged by size:
6369 683 56
BtsI GCAGTG_nn'
Cuts at: 4116 4624 5418 5438 4116
Size: 508 794 20 5786
Fragments arranged by size:
5786 794 508 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7108
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2291 2326 529
Size: 35 1727 35 5311
Fragments arranged by size:
5311 1727 35 35
DraI TTT'AAA
Cuts at: 4412 4412
Size: 7108
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3044 3953 6115 809
Size: 507 1728 909 2162 1802
Fragments arranged by size:
2162 1802 1728 909 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2545 2595 2640
Size: 54 1425 50 275 18 715 50 45
Cuts at: 2640 3366 4555 5444 7073 8
Size: 726 1189 889 1629 43
Fragments arranged by size:
1629 1425 1189 889 726 715 275 54
50 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2545 4555 1537
Size: 275 733 2010 4090
Fragments arranged by size:
4090 2010 733 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2644 3002 3026 3552 3910 5038 1353
Size: 1291 358 24 526 358 1128 3423
Fragments arranged by size:
3423 1291 1128 526 358 358 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5695 6069 6215 387
Size: 1064 138 4106 374 146 1280
Fragments arranged by size:
4106 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 2097 2104 2455 2870 2907 3058
Size: 393 408 57 7 351 415 37 151
Cuts at: 3058 3200 3684 3772 3901 4095 4125 4256 4274
Size: 142 484 88 129 194 30 131 18
Cuts at: 4274 5171 5768 6555 6823 1239
Size: 897 597 787 268 1524
Fragments arranged by size:
1524 897 787 597 484 415 408 393
351 268 194 151 142 131 129 88
57 37 30 18 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2081 2360 2081
Size: 279 6829
EcoO109I rG'GnC_Cy
Cuts at: 2424 3013 3243 3255 3256 3450 3451 3921 4009
Size: 589 230 12 1 194 1 470 88
Cuts at: 4009 4240 2424
Size: 231 5292
Fragments arranged by size:
5292 589 470 231 230 194 88 12
1 1
EcoRI G'AATT_C
Cuts at: 4318 4417 4795 4318
Size: 99 378 6631
Fragments arranged by size:
6631 378 99
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3394 4195 4414 3394
Size: 801 219 6088
Fragments arranged by size:
6088 801 219
FspI TGC'GCA
Cuts at: 5614 5614
Size: 7108
HaeII r_GCGC'y
Cuts at: 1678 2636 2918 3309 6255 1678
Size: 958 282 391 2946 2531
Fragments arranged by size:
2946 2531 958 391 282
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2451 2483 2918 2950 3404
Size: 32 135 32 879 32 435 32 454
Cuts at: 3404 3436 3905 3937 4202 4234 5749 5781 5823
Size: 32 469 32 265 32 1515 32 42
Cuts at: 5823 5855 1373
Size: 32 2626
Fragments arranged by size:
2626 1515 879 469 454 435 265 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4452 4698 669
Size: 988 281 2514 246 3079
Fragments arranged by size:
3079 2514 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7108
HpaI GTT'AAC
Cuts at: 4698 4698
Size: 7108
KasI G'GCGC_C
Cuts at: 3305 3305
Size: 7108
KpnI G_GTAC'C
Cuts at: 2277 3034 4433 2277
Size: 757 1399 4952
Fragments arranged by size:
4952 1399 757
MfeI C'AATT_G
Cuts at: 4707 4707
Size: 7108
MluI A'CGCG_T
Cuts at: 2700 6984 2700
Size: 4284 2824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3969 6222 6406 1305
Size: 2664 2253 184 2007
Fragments arranged by size:
2664 2253 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5286
Fragments arranged by size:
5286 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2807 5066 5425 5584 518
Size: 648 1641 2259 359 159 2042
Fragments arranged by size:
2259 2042 1641 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2457 2475 3151 3553 3783 3928 4073 4524
Size: 851 18 676 402 230 145 145 451
Cuts at: 4524 5201 6349 6594 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 851 677 676 451 402 245
230 145 145 18
NaeI GCC'GGC
Cuts at: 2236 4370 2236
Size: 2134 4974
NarI GG'CG_CC
Cuts at: 3306 3306
Size: 7108
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5617
Fragments arranged by size:
5617 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7108
NgoMIV G'CCGG_C
Cuts at: 2234 4368 2234
Size: 2134 4974
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7108
NotI GC'GGCC_GC
Cuts at: 4555 4555
Size: 7108
NspI r_CATG'y
Cuts at: 1132 1229 3358 4466 6011 1132
Size: 97 2129 1108 1545 2229
Fragments arranged by size:
2229 2129 1545 1108 97
PasI CC'CwG_GG
Cuts at: 1348 3578 1348
Size: 2230 4878
PciI A'CATG_T
Cuts at: 3354 6007 3354
Size: 2653 4455
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2730 4107 4159 1108
Size: 1622 1377 52 4057
Fragments arranged by size:
4057 1622 1377 52
PmeI GTTT'AAAC
Cuts at: 4412 4412
Size: 7108
PmlI CAC'GTG
Cuts at: 2740 2740
Size: 7108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5177 5209 6690 6722 5177
Size: 32 1481 32 5563
Fragments arranged by size:
5563 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3921 4009 4240 3921
Size: 88 231 6789
Fragments arranged by size:
6789 231 88
PshAI GACnn'nnGTC
Cuts at: 1613 3759 1613
Size: 2146 4962
PsiI TTA'TAA
Cuts at: 4678 4678
Size: 7108
PspOMI G'GGCC_C
Cuts at: 2423 3255 3450 2423
Size: 832 195 6081
Fragments arranged by size:
6081 832 195
PstI C_TGCA'G
Cuts at: 830 2082 3053 3120 3150 3432 3486 3991 4460
Size: 1252 971 67 30 282 54 505 469
Cuts at: 4460 830
Size: 3478
Fragments arranged by size:
3478 1252 971 505 469 282 67 54
30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7108
PvuII CAG'CTG
Cuts at: 3151 3553 3783 3928 4073 3151
Size: 402 230 145 145 6186
Fragments arranged by size:
6186 402 230 145 145
SalI G'TCGA_C
Cuts at: 1936 4450 1936
Size: 2514 4594
SanDI GG'GwC_CC
Cuts at: 4009 4009
Size: 7108
SapI GCTCTTCn'nnn_
Cuts at: 3026 3910 3026
Size: 884 6224
SbfI CC_TGCA'GG
Cuts at: 2082 4460 2082
Size: 2378 4730
ScaI AGT'ACT
Cuts at: 5356 7068 5356
Size: 1712 5396
SfcI C'TryA_G
Cuts at: 826 1045 2078 3049 3116 3146 3428 3482 3987
Size: 219 1033 971 67 30 282 54 505
Cuts at: 3987 4083 4456 5591 6272 6463 826
Size: 96 373 1135 681 191 1471
Fragments arranged by size:
1471 1135 1033 971 681 505 373 282
219 191 96 67 54 30
SfoI GGC'GCC
Cuts at: 3307 3307
Size: 7108
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7108
SgrAI Cr'CCGG_yG
Cuts at: 1700 3129 1700
Size: 1429 5679
SmaI CCC'GGG
Cuts at: 1872 2501 2977 3057 4435 1872
Size: 629 476 80 1378 4545
Fragments arranged by size:
4545 1378 629 476 80
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2444 4388 5212 6113 6375 820
Size: 197 926 501 1944 824 901 262 1553
Fragments arranged by size:
1944 1553 926 901 824 501 262 197
SnaBI TAC'GTA
Cuts at: 493 4899 493
Size: 4406 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7108
SphI G_CATG'C
Cuts at: 1132 4466 1132
Size: 3334 3774
SrfI GCCC'GGGC
Cuts at: 3057 3057
Size: 7108
SspI AAT'ATT
Cuts at: 5 52 5032 5
Size: 47 4980 2081
Fragments arranged by size:
4980 2081 47
StuI AGG'CCT
Cuts at: 1517 2432 3122 1517
Size: 915 690 5503
Fragments arranged by size:
5503 915 690
StyI C'CwwG_G
Cuts at: 513 1065 2004 2309 2886 4547 513
Size: 552 939 305 577 1661 3074
Fragments arranged by size:
3074 1661 939 577 552 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3754 4138 4348 5135 5152 5305 5490
Size: 1255 2174 384 210 787 17 153 185
Cuts at: 5490 5935 325
Size: 445 1498
Fragments arranged by size:
2174 1498 1255 787 445 384 210 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5354 7066 96
Size: 274 80 33 51 1443 3377 1712 138
Fragments arranged by size:
3377 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3602 3634 7068 7100 3602
Size: 32 3434 32 3610
Fragments arranged by size:
3610 3434 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2369 3422 1316
Size: 1053 1053 5002
Fragments arranged by size:
5002 1053 1053
XbaI T'CTAG_A
Cuts at: 4444 4444
Size: 7108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4267 4267
Size: 7108
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7108
XmaI C'CCGG_G
Cuts at: 1870 2499 2975 3055 4433 1870
Size: 629 476 80 1378 4545
Fragments arranged by size:
4545 1378 629 476 80
XmnI GAAnn'nnTTC
Cuts at: 2391 5237 2391
Size: 2846 4262
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2857 4197 4916 276
Size: 53 83 186 936 1323 1340 719 2468
Fragments arranged by size:
2468 1340 1323 936 719 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI
PfoI PspXI PsrI RsrII SacI SacII SexAI SfiI
SwaI