Restriction map for
pFN21AE4226
(Linear) (Six-base) MAPSORT of: KIEE4226.seq Check: 5286 from: 1 to: 2403
KIEE4226.orf
With 182 enzymes: SgfI *
March 22, 2012 17:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 308 770 1148 2403
Size: 308 462 378 1255
Fragments arranged by size:
1255 462 378 308
AatII G_ACGT'C
Cuts at: 0 854 2194 2403
Size: 854 1340 209
Fragments arranged by size:
1340 854 209
Acc65I G'GTAC_C
Cuts at: 0 268 1025 2403
Size: 268 757 1378
Fragments arranged by size:
1378 757 268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 99 1053 1767 2090 2403
Size: 35 64 954 714 323 313
Fragments arranged by size:
954 714 323 313 64 35
AflII C'TTAA_G
Cuts at: 0 2383 2403
Size: 2383 20
AflIII A'CryG_T
Cuts at: 0 695 1349 2403
Size: 695 654 1054
Fragments arranged by size:
1054 695 654
AhdI GACnn_n'nnGTC
Cuts at: 0 927 1914 2403
Size: 927 987 489
Fragments arranged by size:
987 927 489
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1059 2403
Size: 1059 1344
AlwNI CAG_nnn'CTG
Cuts at: 0 411 912 1011 1981 2339 2403
Size: 411 501 99 970 358 64
Fragments arranged by size:
970 501 411 358 99 64
ApaI G_GGCC'C
Cuts at: 0 422 1254 1449 2403
Size: 422 832 195 954
Fragments arranged by size:
954 832 422 195
ApaLI G'TGCA_C
Cuts at: 0 805 1135 2403
Size: 805 330 1268
Fragments arranged by size:
1268 805 330
ApoI r'AATT_y
Cuts at: 0 148 2150 2313 2403
Size: 148 2002 163 90
Fragments arranged by size:
2002 163 148 90
AvaI C'yCGr_G
Cuts at: 0 494 970 1050 2403
Size: 494 476 80 1353
Fragments arranged by size:
1353 494 476 80
BamHI G'GATC_C
Cuts at: 0 81 2403
Size: 81 2322
BanI G'GyrC_C
Cuts at: 0 141 268 1025 1104 1300 1613 2403
Size: 141 127 757 79 196 313 790
Fragments arranged by size:
790 757 313 196 141 127 79
BanII G_rGCy'C
Cuts at: 0 25 182 422 1254 1438 1449 1571 1600
Size: 25 157 240 832 184 11 122 29
Cuts at: 1600 1844 2033 2129 2247 2310 2403
Size: 244 189 96 118 63 93
Fragments arranged by size:
832 244 240 189 184 157 122 118
96 93 63 29 25 11
BbeI G_GCGC'C
Cuts at: 0 1304 2403
Size: 1304 1099
BbsI GAAGACnn'nnnn_
Cuts at: 0 1074 2403
Size: 1074 1329
BbvCI CC'TCA_GC
Cuts at: 0 292 937 2403
Size: 292 645 1466
Fragments arranged by size:
1466 645 292
BciVI GTATCCnnnnn_n'
Cuts at: 0 490 2403
Size: 490 1913
BglI GCCn_nnn'nGGC
Cuts at: 0 2266 2403
Size: 2266 137
Bme1580I G_kGCm'C
Cuts at: 0 422 809 1107 1139 1254 1449 1618 2403
Size: 422 387 298 32 115 195 169 785
Fragments arranged by size:
785 422 387 298 195 169 115 32
BmgBI CAC'GTC
Cuts at: 0 903 924 1955 2403
Size: 903 21 1031 448
Fragments arranged by size:
1031 903 448 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 1959 2216 2403
Size: 1959 257 187
Fragments arranged by size:
1959 257 187
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 670 1218 2403
Size: 670 548 1185
Fragments arranged by size:
1185 670 548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 450 865 902 1195 1679 1896 2120
Size: 92 358 415 37 293 484 217 224
Cuts at: 2120 2251 2269 2403
Size: 131 18 134
Fragments arranged by size:
484 415 358 293 224 217 134 131
92 37 18
Bpu10I CC'TnA_GC
Cuts at: 0 292 937 949 2403
Size: 292 645 12 1454
Fragments arranged by size:
1454 645 292 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 2403
Size: 460 1943
BsaI GGTCTCn'nnnn_
Cuts at: 0 338 468 1725 1760 2403
Size: 338 130 1257 35 643
Fragments arranged by size:
1257 643 338 130 35
BsaAI yAC'GTr
Cuts at: 0 735 2403
Size: 735 1668
BsaHI Gr'CG_yC
Cuts at: 0 851 872 1301 1542 2191 2403
Size: 851 21 429 241 649 212
Fragments arranged by size:
851 649 429 241 212 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 446 476 2403
Size: 446 30 1927
Fragments arranged by size:
1927 446 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1233 1778 2009 2403
Size: 1233 545 231 394
Fragments arranged by size:
1233 545 394 231
BseYI C'CCAG_C
Cuts at: 0 184 444 725 1449 1459 1531 1844 1919
Size: 184 260 281 724 10 72 313 75
Cuts at: 1919 2128 2195 2351 2356 2403
Size: 209 67 156 5 47
Fragments arranged by size:
724 313 281 260 209 184 156 75
72 67 47 10 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 925 1294 1405 2099 2403
Size: 217 708 369 111 694 304
Fragments arranged by size:
708 694 369 304 217 111
BsiEI CG_ry'CG
Cuts at: 0 543 2403
Size: 543 1860
BsiHKAI G_wGCw'C
Cuts at: 0 734 809 1139 2403
Size: 734 75 330 1264
Fragments arranged by size:
1264 734 330 75
BsmBI CGTCTCn'nnnn_
Cuts at: 0 880 994 1533 1961 2403
Size: 880 114 539 428 442
Fragments arranged by size:
880 539 442 428 114
Bsp1286I G_dGCh'C
Cuts at: 0 25 182 422 734 809 1107 1139 1254
Size: 25 157 240 312 75 298 32 115
Cuts at: 1254 1438 1449 1571 1600 1618 1844 2033 2129
Size: 184 11 122 29 18 226 189 96
Cuts at: 2129 2247 2310 2403
Size: 118 63 93
Fragments arranged by size:
312 298 240 226 189 184 157 122
118 115 96 93 75 63 32 29
25 18 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 308 770 1148 2403
Size: 308 462 378 1255
Fragments arranged by size:
1255 462 378 308
BsrBI CCG'CTC
Cuts at: 0 1358 1466 2403
Size: 1358 108 937
Fragments arranged by size:
1358 937 108
BsrFI r'CCGG_y
Cuts at: 0 229 1124 1517 2363 2403
Size: 229 895 393 846 40
Fragments arranged by size:
895 846 393 229 40
BssHII G'CGCG_C
Cuts at: 0 704 779 2274 2403
Size: 704 75 1495 129
Fragments arranged by size:
1495 704 129 75
BssSI C'ACGA_G
Cuts at: 0 684 2403
Size: 684 1719
BstAPI GCAn_nnn'nTGC
Cuts at: 0 141 912 2403
Size: 141 771 1491
Fragments arranged by size:
1491 771 141
BstBI TT'CG_AA
Cuts at: 0 50 2403
Size: 50 2353
BstXI CCAn_nnnn'nTGG
Cuts at: 0 882 1099 1997 2229 2403
Size: 882 217 898 232 174
Fragments arranged by size:
898 882 232 217 174
BstYI r'GATC_y
Cuts at: 0 81 2403
Size: 81 2322
Bsu36I CC'TnA_GG
Cuts at: 0 1245 1601 2403
Size: 1245 356 802
Fragments arranged by size:
1245 802 356
BtgI C'CryG_G
Cuts at: 0 1618 2290 2403
Size: 1618 672 113
Fragments arranged by size:
1618 672 113
BtsI GCAGTG_nn'
Cuts at: 0 2111 2403
Size: 2111 292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 286 321 2403
Size: 286 35 2082
Fragments arranged by size:
2082 286 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1039 1948 2403
Size: 1039 909 455
Fragments arranged by size:
1039 909 455
EaeI y'GGCC_r
Cuts at: 0 540 590 635 1361 2403
Size: 540 50 45 726 1042
Fragments arranged by size:
1042 726 540 50 45
EagI C'GGCC_G
Cuts at: 0 540 2403
Size: 540 1863
EarI CTCTTCn'nnn_
Cuts at: 0 639 997 1021 1547 1905 2403
Size: 639 358 24 526 358 498
Fragments arranged by size:
639 526 498 358 358 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 92 99 450 865 902 1053 1195
Size: 35 57 7 351 415 37 151 142
Cuts at: 1195 1679 1767 1896 2090 2120 2251 2269 2403
Size: 484 88 129 194 30 131 18 134
Fragments arranged by size:
484 415 351 194 151 142 134 131
129 88 57 37 35 30 18 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 76 355 2403
Size: 76 279 2048
Fragments arranged by size:
2048 279 76
EcoO109I rG'GnC_Cy
Cuts at: 0 419 1008 1238 1250 1251 1445 1446 1916
Size: 419 589 230 12 1 194 1 470
Cuts at: 1916 2004 2235 2403
Size: 88 231 168
Fragments arranged by size:
589 470 419 231 230 194 168 88
12 1 1
EcoRI G'AATT_C
Cuts at: 0 2313 2403
Size: 2313 90
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1389 2190 2403
Size: 1389 801 213
Fragments arranged by size:
1389 801 213
HaeII r_GCGC'y
Cuts at: 0 631 913 1304 2403
Size: 631 282 391 1099
Fragments arranged by size:
1099 631 391 282
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 446 478 913 945 1399 1431 1900 1932
Size: 446 32 435 32 454 32 469 32
Cuts at: 1932 2197 2229 2403
Size: 265 32 174
Fragments arranged by size:
469 454 446 435 265 174 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 1300 2403
Size: 1300 1103
KpnI G_GTAC'C
Cuts at: 0 272 1029 2403
Size: 272 757 1374
Fragments arranged by size:
1374 757 272
MluI A'CGCG_T
Cuts at: 0 695 2403
Size: 695 1708
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1964 2403
Size: 1964 439
MslI CAynn'nnrTG
Cuts at: 0 802 2403
Size: 802 1601
MspA1I CmG'CkG
Cuts at: 0 452 470 1146 1548 1778 1923 2068 2403
Size: 452 18 676 402 230 145 145 335
Fragments arranged by size:
676 452 402 335 230 145 145 18
NaeI GCC'GGC
Cuts at: 0 231 2365 2403
Size: 231 2134 38
Fragments arranged by size:
2134 231 38
NarI GG'CG_CC
Cuts at: 0 1301 2403
Size: 1301 1102
NgoMIV G'CCGG_C
Cuts at: 0 229 2363 2403
Size: 229 2134 40
Fragments arranged by size:
2134 229 40
NspI r_CATG'y
Cuts at: 0 1353 2403
Size: 1353 1050
PasI CC'CwG_GG
Cuts at: 0 1573 2403
Size: 1573 830
PciI A'CATG_T
Cuts at: 0 1349 2403
Size: 1349 1054
PflMI CCAn_nnn'nTGG
Cuts at: 0 725 2102 2154 2403
Size: 725 1377 52 249
Fragments arranged by size:
1377 725 249 52
PmlI CAC'GTG
Cuts at: 0 735 2403
Size: 735 1668
PpuMI rG'GwC_Cy
Cuts at: 0 1916 2004 2235 2403
Size: 1916 88 231 168
Fragments arranged by size:
1916 231 168 88
PshAI GACnn'nnGTC
Cuts at: 0 1754 2403
Size: 1754 649
PspOMI G'GGCC_C
Cuts at: 0 418 1250 1445 2403
Size: 418 832 195 958
Fragments arranged by size:
958 832 418 195
PstI C_TGCA'G
Cuts at: 0 77 1048 1115 1145 1427 1481 1986 2403
Size: 77 971 67 30 282 54 505 417
Fragments arranged by size:
971 505 417 282 77 67 54 30
PvuII CAG'CTG
Cuts at: 0 1146 1548 1778 1923 2068 2403
Size: 1146 402 230 145 145 335
Fragments arranged by size:
1146 402 335 230 145 145
SanDI GG'GwC_CC
Cuts at: 0 2004 2403
Size: 2004 399
SapI GCTCTTCn'nnn_
Cuts at: 0 1021 1905 2403
Size: 1021 884 498
Fragments arranged by size:
1021 884 498
SbfI CC_TGCA'GG
Cuts at: 0 77 2403
Size: 77 2326
SfcI C'TryA_G
Cuts at: 0 73 1044 1111 1141 1423 1477 1982 2078
Size: 73 971 67 30 282 54 505 96
Cuts at: 2078 2403
Size: 325
Fragments arranged by size:
971 505 325 282 96 73 67 54
30
SfoI GGC'GCC
Cuts at: 0 1302 2403
Size: 1302 1101
SgrAI Cr'CCGG_yG
Cuts at: 0 1124 2403
Size: 1124 1279
SmaI CCC'GGG
Cuts at: 0 496 972 1052 2403
Size: 496 476 80 1351
Fragments arranged by size:
1351 496 476 80
SmlI C'TyrA_G
Cuts at: 0 439 2383 2403
Size: 439 1944 20
Fragments arranged by size:
1944 439 20
SrfI GCCC'GGGC
Cuts at: 0 1052 2403
Size: 1052 1351
StuI AGG'CCT
Cuts at: 0 427 1117 2403
Size: 427 690 1286
Fragments arranged by size:
1286 690 427
StyI C'CwwG_G
Cuts at: 0 304 881 2403
Size: 304 577 1522
Fragments arranged by size:
1522 577 304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1749 2133 2343 2403
Size: 1749 384 210 60
Fragments arranged by size:
1749 384 210 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1597 1629 2403
Size: 1597 32 774
Fragments arranged by size:
1597 774 32
Tth111I GACn'n_nGTC
Cuts at: 0 364 1417 2403
Size: 364 1053 986
Fragments arranged by size:
1053 986 364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2262 2403
Size: 2262 141
XmaI C'CCGG_G
Cuts at: 0 494 970 1050 2403
Size: 494 476 80 1353
Fragments arranged by size:
1353 494 476 80
XmnI GAAnn'nnTTC
Cuts at: 0 386 2403
Size: 386 2017
ZraI GAC'GTC
Cuts at: 0 852 2192 2403
Size: 852 1340 211
Fragments arranged by size:
1340 852 211
Enzymes that do cut:
AarI AatII Acc65I AcuI AflII AflIII AhdI AlfI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BglI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtsI CspCI DrdI EaeI EagI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I KasI
KpnI MluI MmeI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PmlI PpuMI PshAI PspOMI
PstI PvuII SanDI SapI SbfI SfcI SfoI SgrAI
SmaI SmlI SrfI StuI StyI TaqII TstI Tth111I
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AgeI AleI AloI AscI AseI
AsiSI AvrII BaeI BcgI BclI BfrBI BglII BlpI
BmtI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI
BsrGI BstEII BstZ17I BtgZI ClaI DraI DraIII EciI
EcoICRI EcoRV FseI FspI FspAI HincII HindIII HpaI
MfeI MscI NcoI NdeI NheI NotI NruI NsiI
PacI PfoI PmeI PpiI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI ScaI SexAI SfiI SgfI
SnaBI SpeI SphI SspI SwaI TatI XbaI XhoI