################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BbrPI BbsI BfaI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BseAI BsiWI BsmBI Bsp1407I BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII CpoI DraI DraIII DrdI Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FspI HincII HindII HindIII HpaI KpnI MaeI MluI MroI NcoI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI SexAI SfuI SmlI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AocI 1176 CCTCAGG CC^T[ACGT]AGG BalI 1973 TGGCCA TGG^CCA BamHI 861 GGATCC G^GATCC BbeI 73 GGCGCC GGCGC^C BbvCI 1822 CCTCAGC CCTCAGC(-5/-2) BcgI 1098 CGAGAAGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1064 GGATAC GTATCC(6/5) BglII 1376 AGATCT A^GATCT BsaI 760 GAGACC GGTCTC(1/5) BsmI 993 GCATTC GAATGC(1/-1) BspDI 973 ATCGAT AT^CGAT Bsu36I 1176 CCTCAGG CC^T[ACGT]AGG ClaI 973 ATCGAT AT^CGAT Eco81I 1176 CCTCAGG CC^T[ACGT]AGG FseI 732 GGCCGGCC GGCCGG^CC KasI 73 GGCGCC G^GCGCC MaeII 915 ACGT A^CGT MaeIII 917 GTGAC ^GT[ACGT]AC MfeI 1254 CAATTG C^AATTG MluNI 1973 TGGCCA TGG^CCA MscI 1973 TGGCCA TGG^CCA MunI 1254 CAATTG C^AATTG NarI 73 GGCGCC GG^CGCC NotI 1702 GCGGCCGC GC^GGCCGC PflMI 2077 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 382 GACTC GAGTC(4/5) SacI 1994 GAGCTC GAGCT^C ScaI 1949 AGTACT AGT^ACT SfoI 73 GGCGCC GGC^GCC SgrAI 48 CGCCGGCG C[GA]^CCGG[CT]G SphI 1214 GCATGC GCATG^C SspI 2039 AATATT AAT^ATT StuI 1859 AGGCCT AGG^CCT Tsp45I 917 GTGAC ^GT[GC]AC Van91I 2077 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 73 GGCGCC G[GA]^CG[CT]C 226 GACGCC AhdI 628 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2073 GACCCCAGGTC AspEI 628 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2073 GACCCCAGGTC AspHI 1138 GAGCAC G[AT]GC[AT]^C 1994 GAGCTC BanI 73 GGCGCC G^G[CT][GA]CC 697 GGCACC BclI 473 TGATCA T^GATCA 1031 TGATCA BsaHI 73 GGCGCC G[GA]^CG[CT]C 226 GACGCC BsiHKAI 1138 GAGCAC G[AT]GC[AT]^C 1994 GAGCTC BspHI 713 TCATGA T^CATGA 1677 TCATGA DsaI 722 CCGCGG C^C[GA][CT]GG 1706 CCGCGG Eam1105I 628 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2073 GACCCCAGGTC FbaI 473 TGATCA T^GATCA 1031 TGATCA HaeII 73 GGCGCC [GA]GCGC^[CT] 1911 AGCGCC HgaI 214 GACGC GACGC(5/10) 226 GACGC Hin1I 73 GGCGCC G[GA]^CG[CT]C 226 GACGCC KspI 722 CCGCGG CCGC^GG 1706 CCGCGG NaeI 49 GCCGGC GCC^GGC 733 GCCGGC NgoMIV 49 GCCGGC G^CCGGC 733 GCCGGC NspI 1214 GCATGC [GA]CATG^[CT] 1596 ACATGC PstI 775 CTGCAG CTGCA^G 1697 CTGCAG RcaI 713 TCATGA T^CATGA 1677 TCATGA SacII 722 CCGCGG CCGC^GG 1706 CCGCGG ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI ApaI AvaI AvaII BanII BbvI BcnI BglI BmyI BpmI BsaBI BsaJI BsaWI BseRI BsgI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmaI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI