(Linear) (Six-base) MAPSORT of: KIEE2201.seq Check: 8335 from: 1 to: 2133 KIEE2201.orf With 182 enzymes: SgfI * November 2, 2012 16:48 .. AhdI GACnn_n'nnGTC Cuts at: 0 633 2078 2133 Size: 633 1445 55 Fragments arranged by size: 1445 633 55 AleI CACnn'nnGTG Cuts at: 0 283 1304 2133 Size: 283 1021 829 Fragments arranged by size: 1021 829 283 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 994 1685 2133 Size: 994 691 448 Fragments arranged by size: 994 691 448 AlwNI CAG_nnn'CTG Cuts at: 0 598 1531 2083 2133 Size: 598 933 552 50 Fragments arranged by size: 933 598 552 50 ApaI G_GGCC'C Cuts at: 0 100 172 243 655 1097 1124 1550 1565 Size: 100 72 71 412 442 27 426 15 Cuts at: 1565 1610 1768 2133 Size: 45 158 365 Fragments arranged by size: 442 426 412 365 158 100 72 71 45 27 15 AvaI C'yCGr_G Cuts at: 0 31 147 198 562 638 1096 1221 1582 Size: 31 116 51 364 76 458 125 361 Cuts at: 1582 1761 2133 Size: 179 372 Fragments arranged by size: 458 372 364 361 179 125 116 76 51 31 BamHI G'GATC_C Cuts at: 0 861 2133 Size: 861 1272 BanI G'GyrC_C Cuts at: 0 73 697 2133 Size: 73 624 1436 Fragments arranged by size: 1436 624 73 BanII G_rGCy'C Cuts at: 0 100 172 243 591 655 1097 1124 1550 Size: 100 72 71 348 64 442 27 426 Cuts at: 1550 1565 1590 1610 1768 1820 1998 2133 Size: 15 25 20 158 52 178 135 Fragments arranged by size: 442 426 348 178 158 135 100 72 71 64 52 27 25 20 15 BbeI G_GCGC'C Cuts at: 0 77 2133 Size: 77 2056 BbvCI CC'TCA_GC Cuts at: 0 1823 2133 Size: 1823 310 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1087 1121 2133 Size: 1087 34 1012 Fragments arranged by size: 1087 1012 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1058 2133 Size: 1058 1075 BclI T'GATC_A Cuts at: 0 473 1031 2133 Size: 473 558 1102 Fragments arranged by size: 1102 558 473 BglI GCCn_nnn'nGGC Cuts at: 0 104 695 1328 1520 1535 1726 1795 1804 Size: 104 591 633 192 15 191 69 9 Cuts at: 1804 2032 2133 Size: 228 101 Fragments arranged by size: 633 591 228 192 191 104 101 69 15 9 BglII A'GATC_T Cuts at: 0 1376 2133 Size: 1376 757 Bme1580I G_kGCm'C Cuts at: 0 100 172 243 655 989 1097 1124 1550 Size: 100 72 71 412 334 108 27 426 Cuts at: 1550 1565 1610 1768 2133 Size: 15 45 158 365 Fragments arranged by size: 426 412 365 334 158 108 100 72 71 45 27 15 BmrI ACTGGGnnnn_n' Cuts at: 0 1495 1650 2133 Size: 1495 155 483 Fragments arranged by size: 1495 483 155 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 527 809 1153 1184 2133 Size: 527 282 344 31 949 Fragments arranged by size: 949 527 344 282 31 Bpu10I CC'TnA_GC Cuts at: 0 1808 1823 2133 Size: 1808 15 310 Fragments arranged by size: 1808 310 15 BsaI GGTCTCn'nnnn_ Cuts at: 0 754 2133 Size: 754 1379 BsaBI GATnn'nnATC Cuts at: 0 344 715 901 2133 Size: 344 371 186 1232 Fragments arranged by size: 1232 371 344 186 BsaHI Gr'CG_yC Cuts at: 0 74 227 2133 Size: 74 153 1906 Fragments arranged by size: 1906 153 74 BsaWI w'CCGG_w Cuts at: 0 351 938 1978 2133 Size: 351 587 1040 155 Fragments arranged by size: 1040 587 351 155 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1355 1385 2133 Size: 1355 30 748 Fragments arranged by size: 1355 748 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 500 1156 1604 1664 1960 2133 Size: 500 656 448 60 296 173 Fragments arranged by size: 656 500 448 296 173 60 BseYI C'CCAG_C Cuts at: 0 1442 1637 1898 2009 2093 2133 Size: 1442 195 261 111 84 40 Fragments arranged by size: 1442 261 195 111 84 40 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 253 724 895 2133 Size: 253 471 171 1238 Fragments arranged by size: 1238 471 253 171 BsiEI CG_ry'CG Cuts at: 0 164 1323 1706 2133 Size: 164 1159 383 427 Fragments arranged by size: 1159 427 383 164 BsiHKAI G_wGCw'C Cuts at: 0 1142 1998 2133 Size: 1142 856 135 Fragments arranged by size: 1142 856 135 BsmI GAATG_Cn' Cuts at: 0 993 2133 Size: 993 1140 Bsp1286I G_dGCh'C Cuts at: 0 100 172 243 591 655 989 1097 1124 Size: 100 72 71 348 64 334 108 27 Cuts at: 1124 1142 1550 1565 1590 1610 1768 1820 1998 Size: 18 408 15 25 20 158 52 178 Cuts at: 1998 2133 Size: 135 Fragments arranged by size: 408 348 334 178 158 135 108 100 72 71 64 52 27 25 20 18 15 BspHI T'CATG_A Cuts at: 0 713 1677 2133 Size: 713 964 456 Fragments arranged by size: 964 713 456 BsrFI r'CCGG_y Cuts at: 0 49 733 1785 1803 1837 2133 Size: 49 684 1052 18 34 296 Fragments arranged by size: 1052 684 296 49 34 18 BstYI r'GATC_y Cuts at: 0 817 861 952 1040 1376 2133 Size: 817 44 91 88 336 757 Fragments arranged by size: 817 757 336 91 88 44 Bsu36I CC'TnA_GG Cuts at: 0 1177 2133 Size: 1177 956 BtgI C'CryG_G Cuts at: 0 722 1706 2133 Size: 722 984 427 Fragments arranged by size: 984 722 427 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 284 2133 Size: 284 1849 BtsI GCAGTG_nn' Cuts at: 0 576 2133 Size: 576 1557 ClaI AT'CG_AT Cuts at: 0 974 2133 Size: 974 1159 EaeI y'GGCC_r Cuts at: 0 161 1014 1320 1703 1839 1887 1973 2133 Size: 161 853 306 383 136 48 86 160 Fragments arranged by size: 853 383 306 161 160 136 86 48 EagI C'GGCC_G Cuts at: 0 161 1320 1703 2133 Size: 161 1159 383 430 Fragments arranged by size: 1159 430 383 161 EarI CTCTTCn'nnn_ Cuts at: 0 434 1236 1862 2133 Size: 434 802 626 271 Fragments arranged by size: 802 626 434 271 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 80 203 980 2050 2133 Size: 80 123 777 1070 83 Fragments arranged by size: 1070 777 123 83 80 EcoICRI GAG'CTC Cuts at: 0 1996 2133 Size: 1996 137 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 527 809 1153 1184 2133 Size: 527 282 344 31 949 Fragments arranged by size: 949 527 344 282 31 EcoO109I rG'GnC_Cy Cuts at: 0 22 85 168 169 450 651 652 1093 Size: 22 63 83 1 281 201 1 441 Cuts at: 1093 1120 1121 1195 1261 1404 1489 1528 1546 Size: 27 1 74 66 143 85 39 18 Cuts at: 1546 1547 1562 1606 1607 1765 2080 2133 Size: 1 15 44 1 158 315 53 Fragments arranged by size: 441 315 281 201 158 143 85 83 74 66 63 53 44 39 27 22 18 15 1 1 1 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 819 2133 Size: 819 1314 FseI GG_CCGG'CC Cuts at: 0 737 2133 Size: 737 1396 HaeII r_GCGC'y Cuts at: 0 77 1915 2133 Size: 77 1838 218 Fragments arranged by size: 1838 218 77 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 371 403 619 651 937 969 1016 1048 Size: 371 32 216 32 286 32 47 32 Cuts at: 1048 1473 1505 2064 2096 2133 Size: 425 32 559 32 37 Fragments arranged by size: 559 425 371 286 216 47 37 32 32 32 32 32 32 KasI G'GCGC_C Cuts at: 0 73 2133 Size: 73 2060 MfeI C'AATT_G Cuts at: 0 1254 2133 Size: 1254 879 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 314 534 1485 1826 2133 Size: 314 220 951 341 307 Fragments arranged by size: 951 341 314 307 220 MscI TGG'CCA Cuts at: 0 1975 2133 Size: 1975 158 MslI CAynn'nnrTG Cuts at: 0 283 976 1304 2133 Size: 283 693 328 829 Fragments arranged by size: 829 693 328 283 MspA1I CmG'CkG Cuts at: 0 237 551 724 774 1389 1696 1702 1708 Size: 237 314 173 50 615 307 6 6 Cuts at: 1708 2133 Size: 425 Fragments arranged by size: 615 425 314 307 237 173 50 6 6 NaeI GCC'GGC Cuts at: 0 51 735 2133 Size: 51 684 1398 Fragments arranged by size: 1398 684 51 NarI GG'CG_CC Cuts at: 0 74 2133 Size: 74 2059 NgoMIV G'CCGG_C Cuts at: 0 49 733 2133 Size: 49 684 1400 Fragments arranged by size: 1400 684 49 NotI GC'GGCC_GC Cuts at: 0 1703 2133 Size: 1703 430 NspI r_CATG'y Cuts at: 0 1218 1600 2133 Size: 1218 382 533 Fragments arranged by size: 1218 533 382 PasI CC'CwG_GG Cuts at: 0 375 1632 2133 Size: 375 1257 501 Fragments arranged by size: 1257 501 375 PflMI CCAn_nnn'nTGG Cuts at: 0 2083 2133 Size: 2083 50 PfoI T'CCnGG_A Cuts at: 0 908 1989 2133 Size: 908 1081 144 Fragments arranged by size: 1081 908 144 PpuMI rG'GwC_Cy Cuts at: 0 22 450 1195 1261 1404 1489 2080 2133 Size: 22 428 745 66 143 85 591 53 Fragments arranged by size: 745 591 428 143 85 66 53 22 PspOMI G'GGCC_C Cuts at: 0 96 168 239 651 1093 1120 1546 1561 Size: 96 72 71 412 442 27 426 15 Cuts at: 1561 1606 1764 2133 Size: 45 158 369 Fragments arranged by size: 442 426 412 369 158 96 72 71 45 27 15 PstI C_TGCA'G Cuts at: 0 779 1701 2133 Size: 779 922 432 Fragments arranged by size: 922 779 432 PvuII CAG'CTG Cuts at: 0 774 1389 1696 2133 Size: 774 615 307 437 Fragments arranged by size: 774 615 437 307 SacI G_AGCT'C Cuts at: 0 1998 2133 Size: 1998 135 SacII CC_GC'GG Cuts at: 0 725 1709 2133 Size: 725 984 424 Fragments arranged by size: 984 725 424 SanDI GG'GwC_CC Cuts at: 0 450 1261 1404 2133 Size: 450 811 143 729 Fragments arranged by size: 811 729 450 143 ScaI AGT'ACT Cuts at: 0 1951 2133 Size: 1951 182 SfcI C'TryA_G Cuts at: 0 9 775 1697 2019 2133 Size: 9 766 922 322 114 Fragments arranged by size: 922 766 322 114 9 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1520 1535 1795 2133 Size: 1520 15 260 338 Fragments arranged by size: 1520 338 260 15 SfoI GGC'GCC Cuts at: 0 75 2133 Size: 75 2058 SgrAI Cr'CCGG_yG Cuts at: 0 49 2133 Size: 49 2084 SmaI CCC'GGG Cuts at: 0 33 149 200 1223 1584 1763 2133 Size: 33 116 51 1023 361 179 370 Fragments arranged by size: 1023 370 361 179 116 51 33 SphI G_CATG'C Cuts at: 0 1218 2133 Size: 1218 915 SrfI GCCC'GGGC Cuts at: 0 33 149 2133 Size: 33 116 1984 Fragments arranged by size: 1984 116 33 SspI AAT'ATT Cuts at: 0 2041 2133 Size: 2041 92 StuI AGG'CCT Cuts at: 0 1861 2133 Size: 1861 272 StyI C'CwwG_G Cuts at: 0 421 921 1248 2133 Size: 421 500 327 885 Fragments arranged by size: 885 500 421 327 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1410 1848 2133 Size: 1410 438 285 Fragments arranged by size: 1410 438 285 TatI w'GTAC_w Cuts at: 0 443 531 1949 2133 Size: 443 88 1418 184 Fragments arranged by size: 1418 443 184 88 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 618 928 1051 1634 2133 Size: 618 310 123 583 499 Fragments arranged by size: 618 583 499 310 123 XmaI C'CCGG_G Cuts at: 0 31 147 198 1221 1582 1761 2133 Size: 31 116 51 1023 361 179 372 Fragments arranged by size: 1023 372 361 179 116 51 31 Enzymes that do cut: AhdI AleI AlfI AlwNI ApaI AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BglI BglII Bme1580I BmrI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrFI BstYI Bsu36I BtgI BtgZI BtsI ClaI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI FseI HaeII Hin4I KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI ScaI SfcI SfiI SfoI SgrAI SmaI SphI SrfI SspI StuI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI BfrBI BlpI BmgBI BmtI BplI BpuEI BsaAI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FspI FspAI HincII HindIII HpaI KpnI MluI NcoI NdeI NheI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI SexAI SgfI SmlI SnaBI SpeI SwaI TstI Tth111I XbaI XhoI XmnI ZraI