################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BbrPI BbsI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaAI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrGI BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I EarI Eco81I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HinfI HpaI KpnI Ksp632I MaeI MaeII MfeI MluI MluNI MroI MscI MseI MunI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsaI RsrII SalI SapI SbfI ScaI SexAI SfuI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Aor51HI 1004 AGCGCT AGC^GCT AspHI 1360 GAGCTC G[AT]GC[AT]^C AvaII 556 GGACC G^G[AT]CC AviII 1079 TGCGCA TGC^GCA BamHI 1040 GGATCC G^GATCC BbvCI 1420 GCTGAGG CCTCAGC(-5/-2) BcgI 1280 GCACGCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpmI 13 CTGGAG CTGGAG(16/14) BsaBI 1287 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1120 GAGACC GGTCTC(1/5) BsiHKAI 1360 GAGCTC G[AT]GC[AT]^C BsmAI 1120 GAGAC GTCTC(1/5) BspHI 1385 TCATGA T^CATGA BsrDI 271 GCAATG GCAATG(2/0) BstEII 1332 GGTCACC G^GT[ACGT]ACC BstPI 1332 GGTCACC G^GT[ACGT]ACC BstYI 1040 GGATCC [GA]^GATC[CT] DraIII 34 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1004 AGCGCT AGC^GCT EcoNI 1124 CCTTCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1332 GGTCACC G^GT[ACGT]ACC FspI 1079 TGCGCA TGC^GCA MaeIII 1333 GTCAC ^GT[ACGT]AC MamI 1287 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1040 GGATCC [GA]^GATC[CT] MslI 504 CACCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 581 CCATGG C^CATGG NspI 1169 GCATGT [GA]CATG^[CT] PflMI 1088 CCAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1385 TCATGA T^CATGA SacI 1360 GAGCTC GAGCT^C SfiI 685 GGCCCGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 1452 CGCCGGCG C[GA]^CCGG[CT]G TaqI 734 TCGA T^CGA Tsp45I 1333 GTCAC ^GT[GC]AC TspRI 773 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 734 TCGA T^CGA Van91I 1088 CCAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1040 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwI 1041 GATCC GGATC(4/5) 1040 GGATC ApaI 255 GGGCCC GGGCC^C 1446 GGGCCC BlpI 6 GCTGAGC GC^T[ACGT]AGC 66 GCTGAGC Bpu1102I 6 GCTGAGC GC^T[ACGT]AGC 66 GCTGAGC BseRI 955 GAGGAG GAGGAG(10/8) 1423 GAGGAG BsmFI 707 GGGAC GGGAC(10/14) 756 GGGAC BsrFI 142 GCCGGC [GA]^CCGG[CT] 1453 GCCGGC BsrI 249 ACTGG ACTGG(1/-1) 774 ACTGG BssHII 82 GCGCGC G^CGCGC 1227 GCGCGC BstAPI 278 GCATGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 333 GCAGCACCTGC BstXI 696 CCACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 968 CCAAGGAGGTGG CelII 6 GCTGAGC GC^T[ACGT]AGC 66 GCTGAGC Cfr10I 142 GCCGGC [GA]^CCGG[CT] 1453 GCCGGC NaeI 142 GCCGGC GCC^GGC 1453 GCCGGC NgoMIV 142 GCCGGC G^CCGGC 1453 GCCGGC NotI 385 GCGGCCGC GC^GGCCGC 442 GCGGCCGC ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI AvaI BanI BanII BbeI BbvI BcnI BglI BmyI BsaHI BsaJI BsgI BsiEI BsiYI BslI BsoBI Bsp1286I BspMI BsrBI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KasI KspI MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PstI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI StyI TseI XmaCI XmaI