(Circular) (Six-base) MAPSORT of: pFN21AE1249.seq Check: 1856 from: 1 to: 6187 pFN21AE1249 6187 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2352 2352 Size: 6187 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3997 278 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3530 1937 Size: 1593 4594 Acc65I G'GTAC_C Cuts at: 3508 3508 Size: 6187 AclI AA'CG_TT Cuts at: 1563 4314 4687 1563 Size: 2751 373 3063 Fragments arranged by size: 3063 2751 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3026 4250 5634 5902 1632 Size: 1394 1224 1384 268 1917 Fragments arranged by size: 1917 1394 1384 1224 268 AfeI AGC'GCT Cuts at: 3011 3011 Size: 6187 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5990 AflIII A'CryG_T Cuts at: 1203 5086 6063 1203 Size: 3883 977 1327 Fragments arranged by size: 3883 1327 977 AgeI A'CCGG_T Cuts at: 5018 5018 Size: 6187 AhdI GACnn_n'nnGTC Cuts at: 4916 4916 Size: 6187 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6187 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2594 1184 Size: 1410 4777 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3149 3181 3149 Size: 32 6155 AlwNI CAG_nnn'CTG Cuts at: 2344 2482 3259 3930 5502 2344 Size: 138 777 671 1572 3029 Fragments arranged by size: 3029 1572 777 671 138 ApaI G_GGCC'C Cuts at: 2264 3455 2264 Size: 1191 4996 ApaLI G'TGCA_C Cuts at: 4244 5400 4244 Size: 1156 5031 ApoI r'AATT_y Cuts at: 1493 3496 3722 3874 5983 1493 Size: 2003 226 152 2109 1697 Fragments arranged by size: 2109 2003 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4741 160 Size: 872 3709 1606 Fragments arranged by size: 3709 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 AvaI C'yCGr_G Cuts at: 1870 1943 2077 2126 2134 3446 3512 1870 Size: 73 134 49 8 1312 66 4545 Fragments arranged by size: 4545 1312 134 73 66 49 8 BamHI G'GATC_C Cuts at: 3045 3517 3884 3045 Size: 472 367 5348 Fragments arranged by size: 5348 472 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 2130 2195 2296 2312 Size: 325 217 627 335 8 65 101 16 Cuts at: 2312 2639 2857 3402 3461 3508 4963 618 Size: 327 218 545 59 47 1455 1842 Fragments arranged by size: 1842 1455 627 545 335 327 325 218 217 101 65 59 47 16 8 BanII G_rGCy'C Cuts at: 1391 2078 2264 3369 3435 3455 3506 1391 Size: 687 186 1105 66 20 51 4072 Fragments arranged by size: 4072 1105 687 186 66 51 20 BbeI G_GCGC'C Cuts at: 2126 2134 2199 2300 2643 2861 3406 3465 2126 Size: 8 65 101 343 218 545 59 4848 Fragments arranged by size: 4848 545 343 218 101 65 59 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5216 BbvCI CC'TCA_GC Cuts at: 3426 3426 Size: 6187 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3274 3308 4378 4412 1903 Size: 34 1337 34 1070 34 3678 Fragments arranged by size: 3678 1337 1070 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4079 5289 4079 Size: 1210 4977 BclI T'GATC_A Cuts at: 1548 1548 Size: 6187 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2031 2153 2294 Size: 107 122 71 631 605 359 122 141 Cuts at: 2294 2310 2697 3459 4798 136 Size: 16 387 762 1339 1525 Fragments arranged by size: 1525 1339 762 631 605 387 359 141 122 122 107 71 16 BglII A'GATC_T Cuts at: 6182 6182 Size: 6187 BlpI GC'TnA_GC Cuts at: 2012 2072 3604 2012 Size: 60 1532 4595 Fragments arranged by size: 4595 1532 60 Bme1580I G_kGCm'C Cuts at: 1790 2264 3068 3455 4248 5404 1790 Size: 474 804 387 793 1156 2573 Fragments arranged by size: 2573 1156 804 793 474 387 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6187 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3918 4876 442 Size: 416 53 83 399 24 2501 958 1753 Fragments arranged by size: 2501 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6187 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2039 4847 1239 Size: 800 2808 2579 Fragments arranged by size: 2808 2579 800 Bpu10I CC'TnA_GC Cuts at: 3426 3426 Size: 6187 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4312 5177 5475 4312 Size: 865 298 5024 Fragments arranged by size: 5024 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3119 4850 882 Size: 617 1620 1731 2219 Fragments arranged by size: 2219 1731 1620 617 BsaAI yAC'GTr Cuts at: 493 1204 3978 493 Size: 711 2774 2702 Fragments arranged by size: 2774 2702 711 BsaBI GATnn'nnATC Cuts at: 3296 6181 3296 Size: 2885 3302 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2123 2131 2196 2297 Size: 53 83 186 936 590 8 65 101 Cuts at: 2297 2640 2858 3403 3462 3994 4376 5011 275 Size: 343 218 545 59 532 382 635 1451 Fragments arranged by size: 1451 936 635 590 545 532 382 343 218 186 101 83 65 59 53 8 BsaWI w'CCGG_w Cuts at: 4620 5018 5292 5439 5850 4620 Size: 398 274 147 411 4957 Fragments arranged by size: 4957 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3149 3179 1178 Size: 30 165 30 1746 30 4186 Fragments arranged by size: 4186 1746 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2975 3443 1186 Size: 1789 468 3930 Fragments arranged by size: 3930 1789 468 BseYI C'CCAG_C Cuts at: 2708 2758 2863 2939 3420 3434 5390 5886 2708 Size: 50 105 76 481 14 1956 496 3009 Fragments arranged by size: 3009 1956 496 481 105 76 50 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2021 2252 2618 1163 Size: 573 285 231 366 4732 Fragments arranged by size: 4732 573 366 285 231 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2361 2394 2400 2451 2883 2958 Size: 275 186 360 33 6 51 432 75 Cuts at: 2958 3121 3320 3637 4398 5426 1540 Size: 163 199 317 761 1028 2301 Fragments arranged by size: 2301 1028 761 432 360 317 275 199 186 163 75 51 33 6 BsiHKAI G_wGCw'C Cuts at: 3369 4248 4333 5404 3369 Size: 879 85 1071 4152 Fragments arranged by size: 4152 1071 879 85 BsmI GAATG_Cn' Cuts at: 3698 3791 3698 Size: 93 6094 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2078 2264 3068 3369 3435 3455 3506 Size: 399 288 186 804 301 66 20 51 Cuts at: 3506 4248 4333 5404 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 804 742 399 301 288 186 85 66 51 20 BspEI T'CCGG_A Cuts at: 5850 5850 Size: 6187 BspHI T'CATG_A Cuts at: 3390 4076 5806 3390 Size: 686 1730 3771 Fragments arranged by size: 3771 1730 686 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2352 3087 3282 3325 3542 844 Size: 1508 735 195 43 217 3489 Fragments arranged by size: 3489 1508 735 217 195 43 BsrBI CCG'CTC Cuts at: 1394 1823 2353 2499 2527 2828 2928 4074 1394 Size: 429 530 146 28 301 100 1146 3507 Fragments arranged by size: 3507 1146 530 429 301 146 100 28 BsrDI GCAATG_nn' Cuts at: 65 1248 2283 4682 4856 65 Size: 1183 1035 2399 174 1396 Fragments arranged by size: 2399 1396 1183 1035 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2147 3458 4831 5018 1700 Size: 110 337 1311 1373 187 2869 Fragments arranged by size: 2869 1373 1311 337 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6187 BssHII G'CGCG_C Cuts at: 1925 2087 3232 1925 Size: 162 1145 4880 Fragments arranged by size: 4880 1145 162 BssSI C'ACGA_G Cuts at: 4247 5259 4247 Size: 1012 5175 BstAPI GCAn_nnn'nTGC Cuts at: 794 2289 2344 794 Size: 1495 55 4637 Fragments arranged by size: 4637 1495 55 BstBI TT'CG_AA Cuts at: 4991 4991 Size: 6187 BstEII G'GTnAC_C Cuts at: 1184 3337 1184 Size: 2153 4034 BstXI CCAn_nnnn'nTGG Cuts at: 2708 2980 2708 Size: 272 5915 BstYI r'GATC_y Cuts at: 1972 3045 3517 3884 4269 4286 5738 5824 5836 Size: 1073 472 367 385 17 1452 86 12 Cuts at: 5836 6182 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1073 472 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2110 2245 2361 2394 2400 2544 Size: 552 939 106 135 116 33 6 144 Cuts at: 2544 2586 2785 2844 3098 3465 513 Size: 42 199 59 254 367 3235 Fragments arranged by size: 3235 939 552 367 254 199 144 135 116 106 59 42 33 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5448 Fragments arranged by size: 5448 683 56 BtsI GCAGTG_nn' Cuts at: 3703 4497 4517 3703 Size: 794 20 5373 Fragments arranged by size: 5373 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6187 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6152 DraI TTT'AAA Cuts at: 3491 3491 Size: 6187 DraIII CAC_nnn'GTG Cuts at: 2044 2044 Size: 6187 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5194 809 Size: 507 3878 1802 Fragments arranged by size: 3878 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2358 2364 Size: 54 1425 50 275 18 315 213 6 Cuts at: 2364 2391 2397 2403 2419 2448 2487 2679 2880 Size: 27 6 6 16 29 39 192 201 Cuts at: 2880 2955 3118 3317 3468 3634 4523 6152 8 Size: 75 163 199 151 166 889 1629 43 Fragments arranged by size: 1629 1425 889 315 275 213 201 199 192 166 163 151 75 54 50 43 39 29 27 18 16 6 6 6 EagI C'GGCC_G Cuts at: 1537 1812 2358 2391 2397 2448 2880 2955 3118 Size: 275 546 33 6 51 432 75 163 Cuts at: 3118 3317 3634 1537 Size: 199 317 4090 Fragments arranged by size: 4090 546 432 317 275 199 163 75 51 33 6 EarI CTCTTCn'nnn_ Cuts at: 1353 4117 1353 Size: 2764 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2838 3014 4774 5148 5294 387 Size: 1064 138 1249 176 1760 374 146 1280 Fragments arranged by size: 1760 1280 1249 1064 374 176 146 138 EcoICRI GAG'CTC Cuts at: 3367 3367 Size: 6187 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 3026 4250 4847 5634 5902 1239 Size: 393 407 987 1224 597 787 268 1524 Fragments arranged by size: 1524 1224 987 787 597 407 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3133 3133 Size: 6187 EcoO109I rG'GnC_Cy Cuts at: 2032 3451 3452 2032 Size: 1419 1 4767 Fragments arranged by size: 4767 1419 1 EcoRI G'AATT_C Cuts at: 3496 3874 3496 Size: 378 5809 FspI TGC'GCA Cuts at: 3086 4693 3086 Size: 1607 4580 HaeII r_GCGC'y Cuts at: 1678 2126 2134 2199 2300 2537 2643 2844 2861 Size: 448 8 65 101 237 106 201 17 Cuts at: 2861 3013 3185 3259 3406 3465 5334 1678 Size: 152 172 74 147 59 1869 2531 Fragments arranged by size: 2531 1869 448 237 201 172 152 147 106 101 74 65 59 17 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3037 3069 4828 4860 4902 Size: 32 135 32 1465 32 1759 32 42 Cuts at: 4902 4934 1373 Size: 32 2626 Fragments arranged by size: 2626 1759 1465 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3531 3777 669 Size: 988 281 1593 246 3079 Fragments arranged by size: 3079 1593 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6187 HpaI GTT'AAC Cuts at: 3777 3777 Size: 6187 KasI G'GCGC_C Cuts at: 2122 2130 2195 2296 2639 2857 3402 3461 2122 Size: 8 65 101 343 218 545 59 4848 Fragments arranged by size: 4848 545 343 218 101 65 59 8 KpnI G_GTAC'C Cuts at: 3512 3512 Size: 6187 MfeI C'AATT_G Cuts at: 3786 3786 Size: 6187 MluI A'CGCG_T Cuts at: 6063 6063 Size: 6187 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3099 5301 5485 1305 Size: 1794 2202 184 2007 Fragments arranged by size: 2202 2007 1794 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4365 Fragments arranged by size: 4365 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2513 4145 4504 4663 518 Size: 648 1347 1632 359 159 2042 Fragments arranged by size: 2042 1632 1347 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2112 2247 2266 2396 2599 2608 2677 2787 Size: 506 135 19 130 203 9 69 110 Cuts at: 2787 2820 2835 2846 2863 2954 3043 3408 3420 Size: 33 15 11 17 91 89 365 12 Cuts at: 3420 3603 4280 5428 5673 1606 Size: 183 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 506 365 245 203 183 135 130 110 91 89 69 33 19 17 15 12 11 9 NaeI GCC'GGC Cuts at: 2149 3460 2149 Size: 1311 4876 NarI GG'CG_CC Cuts at: 2123 2131 2196 2297 2640 2858 3403 3462 2123 Size: 8 65 101 343 218 545 59 4848 Fragments arranged by size: 4848 545 343 218 101 65 59 8 NcoI C'CATG_G Cuts at: 513 1065 2004 2586 513 Size: 552 939 582 4114 Fragments arranged by size: 4114 939 582 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6187 NgoMIV G'CCGG_C Cuts at: 2147 3458 2147 Size: 1311 4876 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6187 NotI GC'GGCC_GC Cuts at: 2391 2448 3634 2391 Size: 57 1186 4944 Fragments arranged by size: 4944 1186 57 NspI r_CATG'y Cuts at: 1132 1229 3178 3545 5090 1132 Size: 97 1949 367 1545 2229 Fragments arranged by size: 2229 1949 1545 367 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6187 PciI A'CATG_T Cuts at: 5086 5086 Size: 6187 PflMI CCAn_nnn'nTGG Cuts at: 1108 3099 1108 Size: 1991 4196 PmeI GTTT'AAAC Cuts at: 3491 3491 Size: 6187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3149 3181 4256 4288 5769 5801 3149 Size: 32 1075 32 1481 32 3535 Fragments arranged by size: 3535 1481 1075 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6187 PsiI TTA'TAA Cuts at: 3757 3757 Size: 6187 PspOMI G'GGCC_C Cuts at: 2260 3451 2260 Size: 1191 4996 PstI C_TGCA'G Cuts at: 830 2340 3156 3243 3279 3539 830 Size: 1510 816 87 36 260 3478 Fragments arranged by size: 3478 1510 816 260 87 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6187 SacI G_AGCT'C Cuts at: 3369 3369 Size: 6187 SacII CC_GC'GG Cuts at: 2113 2248 2397 2788 2847 2113 Size: 135 149 391 59 5453 Fragments arranged by size: 5453 391 149 135 59 SalI G'TCGA_C Cuts at: 1936 3529 1936 Size: 1593 4594 SbfI CC_TGCA'GG Cuts at: 3539 3539 Size: 6187 ScaI AGT'ACT Cuts at: 4435 6147 4435 Size: 1712 4475 SfcI C'TryA_G Cuts at: 826 1045 2336 3013 3152 3239 3275 3535 4670 Size: 219 1291 677 139 87 36 260 1135 Cuts at: 4670 5351 5542 826 Size: 681 191 1471 Fragments arranged by size: 1471 1291 1135 681 677 260 219 191 139 87 36 SfiI GGCCn_nnn'nGGCC Cuts at: 2697 2697 Size: 6187 SfoI GGC'GCC Cuts at: 2124 2132 2197 2298 2641 2859 3404 3463 2124 Size: 8 65 101 343 218 545 59 4848 Fragments arranged by size: 4848 545 343 218 101 65 59 8 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 SgrAI Cr'CCGG_yG Cuts at: 1700 3458 1700 Size: 1758 4429 SmaI CCC'GGG Cuts at: 1872 2079 2128 2136 3448 3514 1872 Size: 207 49 8 1312 66 4545 Fragments arranged by size: 4545 1312 207 66 49 8 SmlI C'TyrA_G Cuts at: 820 1017 1943 4291 5192 5454 820 Size: 197 926 2348 901 262 1553 Fragments arranged by size: 2348 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3978 493 Size: 3485 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6187 SphI G_CATG'C Cuts at: 1132 3545 1132 Size: 2413 3774 SrfI GCCC'GGGC Cuts at: 2079 2128 2136 2079 Size: 49 8 6130 Fragments arranged by size: 6130 49 8 SspI AAT'ATT Cuts at: 5 52 4111 5 Size: 47 4059 2081 Fragments arranged by size: 4059 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6187 StyI C'CwwG_G Cuts at: 513 1065 2004 2586 2973 3324 3480 3626 513 Size: 552 939 582 387 351 156 146 3074 Fragments arranged by size: 3074 939 582 552 387 351 156 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4214 4231 4384 4569 5014 325 Size: 1255 2634 17 153 185 445 1498 Fragments arranged by size: 2634 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4433 6145 96 Size: 274 80 33 51 1443 2456 1712 138 Fragments arranged by size: 2456 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6147 6179 6147 Size: 32 6155 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6187 XbaI T'CTAG_A Cuts at: 3523 3523 Size: 6187 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6187 XmaI C'CCGG_G Cuts at: 1870 2077 2126 2134 3446 3512 1870 Size: 207 49 8 1312 66 4545 Fragments arranged by size: 4545 1312 207 66 49 8 XmnI GAAnn'nnTTC Cuts at: 4316 4316 Size: 6187 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3995 276 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SwaI XcmI