(Linear) (Six-base) MAPSORT of: KIEE1249.seq Check: 4070 from: 1 to: 1482 KIEE1249.orf With 182 enzymes: SgfI * December 5, 2014 12:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 347 1482 Size: 347 1135 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1021 1482 Size: 1021 461 AfeI AGC'GCT Cuts at: 0 1006 1482 Size: 1006 476 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 589 1482 Size: 589 893 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1144 1176 1482 Size: 1144 32 306 Fragments arranged by size: 1144 306 32 AlwNI CAG_nnn'CTG Cuts at: 0 339 477 1254 1482 Size: 339 138 777 228 Fragments arranged by size: 777 339 228 138 ApaI G_GGCC'C Cuts at: 0 259 1450 1482 Size: 259 1191 32 Fragments arranged by size: 1191 259 32 AvaI C'yCGr_G Cuts at: 0 72 121 129 1441 1482 Size: 72 49 8 1312 41 Fragments arranged by size: 1312 72 49 41 8 BamHI G'GATC_C Cuts at: 0 1040 1482 Size: 1040 442 BanI G'GyrC_C Cuts at: 0 117 125 190 291 307 634 852 1397 Size: 117 8 65 101 16 327 218 545 Cuts at: 1397 1456 1482 Size: 59 26 Fragments arranged by size: 545 327 218 117 101 65 59 26 16 8 BanII G_rGCy'C Cuts at: 0 73 259 1364 1430 1450 1482 Size: 73 186 1105 66 20 32 Fragments arranged by size: 1105 186 73 66 32 20 BbeI G_GCGC'C Cuts at: 0 121 129 194 295 638 856 1401 1460 Size: 121 8 65 101 343 218 545 59 Cuts at: 1460 1482 Size: 22 Fragments arranged by size: 545 343 218 121 101 65 59 22 8 BbvCI CC'TCA_GC Cuts at: 0 1421 1482 Size: 1421 61 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1269 1303 1482 Size: 1269 34 179 Fragments arranged by size: 1269 179 34 BglI GCCn_nnn'nGGC Cuts at: 0 26 148 289 305 692 1454 1482 Size: 26 122 141 16 387 762 28 Fragments arranged by size: 762 387 141 122 28 26 16 BlpI GC'TnA_GC Cuts at: 0 7 67 1482 Size: 7 60 1415 Fragments arranged by size: 1415 60 7 Bme1580I G_kGCm'C Cuts at: 0 259 1063 1450 1482 Size: 259 804 387 32 Fragments arranged by size: 804 387 259 32 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 1482 Size: 34 1448 Bpu10I CC'TnA_GC Cuts at: 0 1421 1482 Size: 1421 61 BsaI GGTCTCn'nnnn_ Cuts at: 0 1114 1482 Size: 1114 368 BsaBI GATnn'nnATC Cuts at: 0 1291 1482 Size: 1291 191 BsaHI Gr'CG_yC Cuts at: 0 118 126 191 292 635 853 1398 1457 Size: 118 8 65 101 343 218 545 59 Cuts at: 1457 1482 Size: 25 Fragments arranged by size: 545 343 218 118 101 65 59 25 8 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1144 1174 1482 Size: 1144 30 308 Fragments arranged by size: 1144 308 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 970 1438 1482 Size: 970 468 44 Fragments arranged by size: 970 468 44 BseYI C'CCAG_C Cuts at: 0 703 753 858 934 1415 1429 1482 Size: 703 50 105 76 481 14 53 Fragments arranged by size: 703 481 105 76 53 50 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 16 247 613 1482 Size: 16 231 366 869 Fragments arranged by size: 869 366 231 16 BsiEI CG_ry'CG Cuts at: 0 356 389 395 446 878 953 1116 1315 Size: 356 33 6 51 432 75 163 199 Cuts at: 1315 1482 Size: 167 Fragments arranged by size: 432 356 199 167 163 75 51 33 6 BsiHKAI G_wGCw'C Cuts at: 0 1364 1482 Size: 1364 118 Bsp1286I G_dGCh'C Cuts at: 0 73 259 1063 1364 1430 1450 1482 Size: 73 186 804 301 66 20 32 Fragments arranged by size: 804 301 186 73 66 32 20 BspHI T'CATG_A Cuts at: 0 1385 1482 Size: 1385 97 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 347 1082 1277 1320 1482 Size: 347 735 195 43 162 Fragments arranged by size: 735 347 195 162 43 BsrBI CCG'CTC Cuts at: 0 348 494 522 823 923 1482 Size: 348 146 28 301 100 559 Fragments arranged by size: 559 348 301 146 100 28 BsrDI GCAATG_nn' Cuts at: 0 278 1482 Size: 278 1204 BsrFI r'CCGG_y Cuts at: 0 142 1453 1482 Size: 142 1311 29 Fragments arranged by size: 1311 142 29 BssHII G'CGCG_C Cuts at: 0 82 1227 1482 Size: 82 1145 255 Fragments arranged by size: 1145 255 82 BstAPI GCAn_nnn'nTGC Cuts at: 0 284 339 1482 Size: 284 55 1143 Fragments arranged by size: 1143 284 55 BstEII G'GTnAC_C Cuts at: 0 1332 1482 Size: 1332 150 BstXI CCAn_nnnn'nTGG Cuts at: 0 703 975 1482 Size: 703 272 507 Fragments arranged by size: 703 507 272 BstYI r'GATC_y Cuts at: 0 1040 1482 Size: 1040 442 BtgI C'CryG_G Cuts at: 0 105 240 356 389 395 539 581 780 Size: 105 135 116 33 6 144 42 199 Cuts at: 780 839 1093 1460 1482 Size: 59 254 367 22 Fragments arranged by size: 367 254 199 144 135 116 105 59 42 33 22 6 DraIII CAC_nnn'GTG Cuts at: 0 39 1482 Size: 39 1443 EaeI y'GGCC_r Cuts at: 0 140 353 359 386 392 398 414 443 Size: 140 213 6 27 6 6 16 29 Cuts at: 443 482 674 875 950 1113 1312 1463 1482 Size: 39 192 201 75 163 199 151 19 Fragments arranged by size: 213 201 199 192 163 151 140 75 39 29 27 19 16 6 6 6 EagI C'GGCC_G Cuts at: 0 353 386 392 443 875 950 1113 1312 Size: 353 33 6 51 432 75 163 199 Cuts at: 1312 1482 Size: 170 Fragments arranged by size: 432 353 199 170 163 75 51 33 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 833 1009 1482 Size: 833 176 473 Fragments arranged by size: 833 473 176 EcoICRI GAG'CTC Cuts at: 0 1362 1482 Size: 1362 120 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 1021 1482 Size: 34 987 461 Fragments arranged by size: 987 461 34 EcoNI CCTnn'n_nnAGG Cuts at: 0 1128 1482 Size: 1128 354 EcoO109I rG'GnC_Cy Cuts at: 0 27 1446 1447 1482 Size: 27 1419 1 35 Fragments arranged by size: 1419 35 27 1 FspI TGC'GCA Cuts at: 0 1081 1482 Size: 1081 401 HaeII r_GCGC'y Cuts at: 0 121 129 194 295 532 638 839 856 Size: 121 8 65 101 237 106 201 17 Cuts at: 856 1008 1180 1254 1401 1460 1482 Size: 152 172 74 147 59 22 Fragments arranged by size: 237 201 172 152 147 121 106 101 74 65 59 22 17 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1032 1064 1482 Size: 1032 32 418 Fragments arranged by size: 1032 418 32 KasI G'GCGC_C Cuts at: 0 117 125 190 291 634 852 1397 1456 Size: 117 8 65 101 343 218 545 59 Cuts at: 1456 1482 Size: 26 Fragments arranged by size: 545 343 218 117 101 65 59 26 8 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1094 1482 Size: 1094 388 MslI CAynn'nnrTG Cuts at: 0 508 1482 Size: 508 974 MspA1I CmG'CkG Cuts at: 0 107 242 261 391 594 603 672 782 Size: 107 135 19 130 203 9 69 110 Cuts at: 782 815 830 841 858 949 1038 1403 1415 Size: 33 15 11 17 91 89 365 12 Cuts at: 1415 1482 Size: 67 Fragments arranged by size: 365 203 135 130 110 107 91 89 69 67 33 19 17 15 12 11 9 NaeI GCC'GGC Cuts at: 0 144 1455 1482 Size: 144 1311 27 Fragments arranged by size: 1311 144 27 NarI GG'CG_CC Cuts at: 0 118 126 191 292 635 853 1398 1457 Size: 118 8 65 101 343 218 545 59 Cuts at: 1457 1482 Size: 25 Fragments arranged by size: 545 343 218 118 101 65 59 25 8 NcoI C'CATG_G Cuts at: 0 581 1482 Size: 581 901 NgoMIV G'CCGG_C Cuts at: 0 142 1453 1482 Size: 142 1311 29 Fragments arranged by size: 1311 142 29 NotI GC'GGCC_GC Cuts at: 0 386 443 1482 Size: 386 57 1039 Fragments arranged by size: 1039 386 57 NspI r_CATG'y Cuts at: 0 1173 1482 Size: 1173 309 PflMI CCAn_nnn'nTGG Cuts at: 0 1094 1482 Size: 1094 388 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1144 1176 1482 Size: 1144 32 306 Fragments arranged by size: 1144 306 32 PspOMI G'GGCC_C Cuts at: 0 255 1446 1482 Size: 255 1191 36 Fragments arranged by size: 1191 255 36 PstI C_TGCA'G Cuts at: 0 335 1151 1238 1274 1482 Size: 335 816 87 36 208 Fragments arranged by size: 816 335 208 87 36 SacI G_AGCT'C Cuts at: 0 1364 1482 Size: 1364 118 SacII CC_GC'GG Cuts at: 0 108 243 392 783 842 1482 Size: 108 135 149 391 59 640 Fragments arranged by size: 640 391 149 135 108 59 SfcI C'TryA_G Cuts at: 0 331 1008 1147 1234 1270 1482 Size: 331 677 139 87 36 212 Fragments arranged by size: 677 331 212 139 87 36 SfiI GGCCn_nnn'nGGCC Cuts at: 0 692 1482 Size: 692 790 SfoI GGC'GCC Cuts at: 0 119 127 192 293 636 854 1399 1458 Size: 119 8 65 101 343 218 545 59 Cuts at: 1458 1482 Size: 24 Fragments arranged by size: 545 343 218 119 101 65 59 24 8 SgrAI Cr'CCGG_yG Cuts at: 0 1453 1482 Size: 1453 29 SmaI CCC'GGG Cuts at: 0 74 123 131 1443 1482 Size: 74 49 8 1312 39 Fragments arranged by size: 1312 74 49 39 8 SrfI GCCC'GGGC Cuts at: 0 74 123 131 1482 Size: 74 49 8 1351 Fragments arranged by size: 1351 74 49 8 StyI C'CwwG_G Cuts at: 0 581 968 1319 1475 1482 Size: 581 387 351 156 7 Fragments arranged by size: 581 387 351 156 7 XmaI C'CCGG_G Cuts at: 0 72 121 129 1441 1482 Size: 72 49 8 1312 41 Fragments arranged by size: 1312 72 49 41 8 Enzymes that do cut: AarI AcuI AfeI AlfI AloI AlwNI ApaI AvaI BamHI BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I BpmI Bpu10I BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstEII BstXI BstYI BtgI DraIII EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PflMI PpiI PspOMI PstI SacI SacII SfcI SfiI SfoI SgrAI SmaI SrfI StyI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI BciVI BclI BfrBI BglII BmgBI BmrI BmtI BplI BpuEI BsaAI BsaWI BsiWI BsmI BsmBI BspEI BsrGI BssSI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EarI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NdeI NheI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SexAI SgfI SmlI SnaBI SpeI SphI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI