################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BsaAI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI KpnI MaeI MaeII MfeI MflI MluI MluNI MroI MscI MslI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccII 31 CGCG CG^CG AflIII 437 ACATGT A^C[GA][CT]GT ApaI 261 GGGCCC GGGCC^C AseI 462 ATTAAT AT^TAAT AsnI 462 ATTAAT AT^TAAT AspHI 138 GAGCTC G[AT]GC[AT]^C AvaI 143 CCCGGG C^[CT]CG[GA]G AvaII 272 GGTCC G^G[AT]CC BbeI 34 GGCGCC GGCGC^C BbsI 105 GAAGAC GAAGAC(2/6) BcgI 388 GCAGAAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 542 GCTTAGC GC^T[ACGT]AGC BpmI 565 CTCCAG CTGGAG(16/14) Bpu1102I 542 GCTTAGC GC^T[ACGT]AGC BpuAI 105 GAAGAC GAAGAC(2/6) BsaBI 231 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 364 GGTCTC GGTCTC(1/5) BseRI 448 GAGGAG GAGGAG(10/8) BsgI 352 GTGCAG GTGCAG(16/14) BsiHKAI 138 GAGCTC G[AT]GC[AT]^C BsoBI 143 CCCGGG C^[CT]CG[GA]G BspLU11I 437 ACATGT A^CATGT BsrDI 483 CATTGC GCAATG(2/0) BsrFI 10 GCCGGT [GA]^CCGG[CT] BstUI 31 CGCG CG^CG CelII 542 GCTTAGC GC^T[ACGT]AGC Cfr10I 10 GCCGGT [GA]^CCGG[CT] DpnII 231 GATC ^GATC DraII 261 GGGCCCC [GA]G^G[ACGT]CC[CT] EaeI 8 TGGCCG [CT]^GGCC[GA] EarI 409 CTCTTC CTCTTC(1/4) EcoO109I 261 GGGCCCC [GA]G^G[ACGT]CC[CT] FauI 18 GCGGG CCCGC(4/6) KasI 34 GGCGCC G^GCGCC Ksp632I 409 CTCTTC CTCTTC(1/4) KspI 30 CCGCGG CCGC^GG MamI 231 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 231 GATC ^GATC MseI 463 TTAA T^TAA MspA1I 30 CCGCGG C[AC]G^C[GT]G MvnI 31 CGCG CG^CG NarI 34 GGCGCC GG^CGCC NdeII 231 GATC ^GATC NspI 437 ACATGT [GA]CATG^[CT] PshBI 462 ATTAAT AT^TAAT SacI 138 GAGCTC GAGCT^C SacII 30 CCGCGG CCGC^GG Sau3AI 231 GATC ^GATC SfoI 34 GGCGCC GGC^GCC SmaI 143 CCCGGG CCC^GGG SmlI 24 CTTGAG C^T[CT][GA]AG TaqI 422 TCGA T^CGA Tru9I 463 TTAA T^TAA TspRI 537 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 422 TCGA T^CGA XmaCI 143 CCCGGG C^CCGGG XmaI 143 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BanII 138 GAGCTC G[GA]GC[CT]^C 261 GGGCCC BsmAI 77 GTCTC GTCTC(1/5) 365 GTCTC BsrI 538 ACTGG ACTGG(1/-1) 275 CCAGT Cac8I 18 GCGGGC GC[ACGT]^[ACGT]GC 555 GCTGGC CfoI 35 GCGC GCG^C 524 GCGC DdeI 360 CTTAG C^T[ACGT]AG 543 CTTAG HaeII 34 GGCGCC [GA]GCGC^[CT] 523 AGCGCC HhaI 35 GCGC GCG^C 524 GCGC HinP1I 35 GCGC G^CGC 524 GCGC MaeIII 278 GTTAC ^GT[ACGT]AC 434 GTTAC MboII 105 GAAGA GAAGA(8/7) 410 TCTTC MwoI 42 GCTACGGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 555 GCTGGCCTGGC NlaIII 258 CATG CATG^ 438 CATG PstI 127 CTGCAG CTGCA^G 374 CTGCAG SfaNI 343 GCATC GCATC(5/9) 394 GCATC SfcI 127 CTGCAG C^T[GA][CT]AG 374 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AcyI AluI BanI BbvI BcnI BmyI BsaHI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cfr13I DsaI EcoRII Fnu4HI FokI HaeIII HapII HgaI Hin1I HpaII HphI ItaI MnlI MspI MvaI NciI NlaIV Sau96I ScrFI TseI