################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI AhdI ApoI AscI AseI AsnI Asp718I AspEI BcgI BciVI BfrI BglII BsiWI BsmBI BsmI BsrBI BssHII Bst1107I BstBI BstZ17I CpoI DraI Eam1105I EcoRI EcoRV HpaI KpnI MfeI MluI MunI NheI NotI NruI NspV PacI PinAI PmeI PshAI PshBI Psp1406I RsrII SfuI SgrAI SnaBI SpeI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 547 GTCGAC GT^[AC][GT]AC AccIII 4403 TCCGGA T^CCGGA Alw44I 18 GTGCAC G^TGCAC ApaLI 18 GTGCAC G^TGCAC AspI 3205 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 3059 TGCGCA TGC^GCA AvrII 3459 CCTAGG C^CTAGG BamHI 249 GGATCC G^GATCC BbeI 3463 GGCGCC GGCGC^C BclI 413 TGATCA T^GATCA BlnI 3459 CCTAGG C^CTAGG BsaWI 4403 TCCGGA [AT]^CCGG[AT] BseAI 4403 TCCGGA T^CCGGA BsgI 2995 GTGCAG GTGCAG(16/14) BspDI 4537 ATCGAT AT^CGAT BspEI 4403 TCCGGA T^CCGGA ClaI 4537 ATCGAT AT^CGAT EagI 4604 CGGCCG C^GGCCG EclXI 4604 CGGCCG C^GGCCG Eco52I 4604 CGGCCG C^GGCCG EcoT22I 2230 ATGCAT ATGCA^T FbaI 413 TGATCA T^GATCA FseI 2316 GGCCGGCC GGCCGG^CC FspI 3059 TGCGCA TGC^GCA HindIII 3973 AAGCTT A^AGCTT KasI 3463 GGCGCC G^GCGCC KspI 4386 CCGCGG CCGC^GG MroI 4403 TCCGGA T^CCGGA NarI 3463 GGCGCC GG^CGCC NsiI 2230 ATGCAT ATGCA^T PaeR7I 2719 CTCGAG C^TCGAG PflFI 3205 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuI 713 CGATCG CGAT^CG SacI 2406 GAGCTC GAGCT^C SacII 4386 CCGCGG CCGC^GG SalI 547 GTCGAC G^TCGAC SapI 810 GCTCTTC GCTCTTC(1/4) SexAI 1841 ACCTGGT A^CC[AT]GGT SfoI 3463 GGCGCC GGC^GCC SphI 2153 GCATGC GCATG^C TfiI 3425 GATTC G^A[AT]TC Tth111I 3205 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2719 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### Aor51HI 2999 AGCGCT AGC^GCT 5231 AGCGCT BbvCI 1953 CCTCAGC CCTCAGC(-5/-2) 4950 GCTGAGG BsiEI 713 CGATCG CG[GA][CT]^CG 4604 CGGCCG Bsp1407I 4154 TGTACA T^GTACA 4775 TGTACA BsrGI 4154 TGTACA T^GTACA 4775 TGTACA BstEII 2101 GGTAACC G^GT[ACGT]ACC 4101 GGTCACC BstPI 2101 GGTAACC G^GT[ACGT]ACC 4101 GGTCACC DrdI 589 GACTTCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4258 GACTTCGTGGTC Eco47III 2999 AGCGCT AGC^GCT 5231 AGCGCT EcoO65I 2101 GGTAACC G^GT[ACGT]ACC 4101 GGTCACC MseI 2735 TTAA T^TAA 3266 TTAA NdeI 1346 CATATG CA^TATG 1668 CATATG SbfI 3711 CCTGCAGG CCTGCA^GG 4938 CCTGCAGG ScaI 4226 AGTACT AGT^ACT 4316 AGTACT SfiI 1329 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1365 GGCCACCCTGGCC Sse8387I 3711 CCTGCAGG CCTGCA^GG 4938 CCTGCAGG SspBI 4154 TGTACA T^GTACA 4775 TGTACA Tru9I 2735 TTAA T^TAA 3266 TTAA Tsp509I 2083 AATT ^AATT 4002 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 143 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI Asp700I AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI