(Circular) (Six-base) MAPSORT of: pFN21AE2441.seq Check: 1973 from: 1 to: 10213 pFN21AE2441 10213 bp With 182 enzymes: SgfI * January 7, 2013 17:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2338 5710 7236 2338 Size: 3372 1526 5315 Fragments arranged by size: 5315 3372 1526 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8023 278 Size: 53 83 186 936 6487 2468 Fragments arranged by size: 6487 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2553 7556 1937 Size: 616 5003 4594 Fragments arranged by size: 5003 4594 616 Acc65I G'GTAC_C Cuts at: 7534 7534 Size: 10213 AclI AA'CG_TT Cuts at: 1563 8340 8713 1563 Size: 6777 373 3063 Fragments arranged by size: 6777 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2338 2581 2732 3163 3719 3905 4402 4685 Size: 706 243 151 431 556 186 497 283 Cuts at: 4685 5509 8276 9660 9928 1632 Size: 824 2767 1384 268 1917 Fragments arranged by size: 2767 1917 1384 824 706 556 497 431 283 268 243 186 151 AfeI AGC'GCT Cuts at: 5006 7238 5006 Size: 2232 7981 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10016 AflIII A'CryG_T Cuts at: 1203 2898 2970 5814 6777 7260 9112 10089 1203 Size: 1695 72 2844 963 483 1852 977 1327 Fragments arranged by size: 2844 1852 1695 1327 977 963 483 72 AgeI A'CCGG_T Cuts at: 9044 9044 Size: 10213 AhdI GACnn_n'nnGTC Cuts at: 8942 8942 Size: 10213 AleI CACnn'nnGTG Cuts at: 1166 2030 3643 1166 Size: 864 1613 7736 Fragments arranged by size: 7736 1613 864 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2048 1184 Size: 864 9349 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7100 7132 7100 Size: 32 10181 AlwNI CAG_nnn'CTG Cuts at: 3997 4844 5073 7448 7956 9528 3997 Size: 847 229 2375 508 1572 4682 Fragments arranged by size: 4682 2375 1572 847 508 229 ApaI G_GGCC'C Cuts at: 2045 2683 2731 5404 6654 2045 Size: 638 48 2673 1250 5604 Fragments arranged by size: 5604 2673 1250 638 48 ApaLI G'TGCA_C Cuts at: 2023 8270 9426 2023 Size: 6247 1156 2810 Fragments arranged by size: 6247 2810 1156 ApoI r'AATT_y Cuts at: 1493 7522 7748 7900 10009 1493 Size: 6029 226 152 2109 1697 Fragments arranged by size: 6029 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 8767 160 Size: 872 7735 1606 Fragments arranged by size: 7735 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10213 AvaI C'yCGr_G Cuts at: 1870 1943 2067 2538 3562 4577 4628 4652 4724 Size: 73 124 471 1024 1015 51 24 72 Cuts at: 4724 4922 4958 5772 6682 7538 1870 Size: 198 36 814 910 856 4545 Fragments arranged by size: 4545 1024 1015 910 856 814 471 198 124 73 72 51 36 24 AvrII C'CTAG_G Cuts at: 5464 5464 Size: 10213 BamHI G'GATC_C Cuts at: 2254 7543 7910 2254 Size: 5289 367 4557 Fragments arranged by size: 5289 4557 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2032 4774 5468 6686 7009 Size: 325 217 627 245 2742 694 1218 323 Cuts at: 7009 7224 7534 8989 618 Size: 215 310 1455 1842 Fragments arranged by size: 2742 1842 1455 1218 694 627 325 323 310 245 217 215 BanII G_rGCy'C Cuts at: 1391 2045 2154 2351 2683 2731 3065 3308 3562 Size: 654 109 197 332 48 334 243 254 Cuts at: 3562 4415 4556 5146 5404 6627 6654 6837 7155 Size: 853 141 590 258 1223 27 183 318 Cuts at: 7155 7532 1391 Size: 377 4072 Fragments arranged by size: 4072 1223 853 654 590 377 334 332 318 258 254 243 197 183 141 109 48 27 BbeI G_GCGC'C Cuts at: 5472 5472 Size: 10213 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2282 2720 2770 3844 4024 4258 4339 Size: 971 383 438 50 1074 180 234 81 Cuts at: 4339 5110 5209 928 Size: 771 99 5932 Fragments arranged by size: 5932 1074 971 771 438 383 234 180 99 81 50 BbvCI CC'TCA_GC Cuts at: 3959 6956 3959 Size: 2997 7216 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8404 8438 1903 Size: 34 6467 34 3678 Fragments arranged by size: 6467 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8105 9315 8105 Size: 1210 9003 BclI T'GATC_A Cuts at: 1548 2418 1548 Size: 870 9343 BfrBI ATG'CAT Cuts at: 4237 4237 Size: 10213 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3194 3341 3377 Size: 107 122 71 631 605 1522 147 36 Cuts at: 3377 4228 4601 7281 8824 136 Size: 851 373 2680 1543 1525 Fragments arranged by size: 2680 1543 1525 1522 851 631 605 373 147 122 107 71 36 BglII A'GATC_T Cuts at: 10208 10208 Size: 10213 BlpI GC'TnA_GC Cuts at: 2345 4409 4571 5132 7630 2345 Size: 2064 162 561 2498 4928 Fragments arranged by size: 4928 2498 2064 561 162 Bme1580I G_kGCm'C Cuts at: 1790 2027 2045 2683 2731 3108 3638 4779 5404 Size: 237 18 638 48 377 530 1141 625 Cuts at: 5404 5422 6654 6689 8274 9430 1790 Size: 18 1232 35 1585 1156 2573 Fragments arranged by size: 2573 1585 1232 1156 1141 638 625 530 377 237 48 35 18 18 BmgBI CAC'GTC Cuts at: 1318 6606 1318 Size: 5288 4925 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6895 7944 8902 Size: 416 53 83 399 24 5478 1049 958 Cuts at: 8902 442 Size: 1753 Fragments arranged by size: 5478 1753 1049 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10213 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2264 3077 3470 4626 4823 6695 6818 8873 Size: 1025 813 393 1156 197 1872 123 2055 Cuts at: 8873 1239 Size: 2579 Fragments arranged by size: 2579 2055 1872 1156 1025 813 393 197 123 Bpu10I CC'TnA_GC Cuts at: 2786 3959 4550 6956 2786 Size: 1173 591 2406 6043 Fragments arranged by size: 6043 2406 1173 591 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3440 6128 6194 7079 7154 8338 9203 9501 3440 Size: 2688 66 885 75 1184 865 298 4152 Fragments arranged by size: 4152 2688 1184 885 865 298 75 66 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2205 4289 5099 6557 8876 882 Size: 617 706 2084 810 1458 2319 2219 Fragments arranged by size: 2319 2219 2084 1458 810 706 617 BsaAI yAC'GTr Cuts at: 493 1204 2883 2901 2973 5023 8004 493 Size: 711 1679 18 72 2050 2981 2702 Fragments arranged by size: 2981 2702 2050 1679 711 72 18 BsaBI GATnn'nnATC Cuts at: 2423 4478 5747 6089 6179 7430 10207 2423 Size: 2055 1269 342 90 1251 2777 2429 Fragments arranged by size: 2777 2429 2055 1269 1251 342 90 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3021 3600 5469 5880 Size: 53 83 186 936 1488 579 1869 411 Cuts at: 5880 6894 7089 7296 7404 8020 8402 9037 275 Size: 1014 195 207 108 616 382 635 1451 Fragments arranged by size: 1869 1488 1451 1014 936 635 616 579 411 382 207 195 186 108 83 53 BsaWI w'CCGG_w Cuts at: 6408 8646 9044 9318 9465 9876 6408 Size: 2238 398 274 147 411 6745 Fragments arranged by size: 6745 2238 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4178 4208 7100 7130 1178 Size: 30 165 30 2775 30 2892 30 4261 Fragments arranged by size: 4261 2892 2775 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 2123 2162 3413 3416 3458 4670 4805 Size: 934 3 39 1251 3 42 1212 135 Cuts at: 4805 4832 4940 4952 4964 4991 5798 6455 6458 Size: 27 108 12 12 27 807 657 3 Cuts at: 6458 6617 6836 7124 7199 7397 1186 Size: 159 219 288 75 198 4002 Fragments arranged by size: 4002 1251 1212 934 807 657 288 219 198 159 135 108 75 42 39 27 27 12 12 3 3 3 BseYI C'CCAG_C Cuts at: 2991 3101 3183 3502 4102 5353 5368 6615 9416 Size: 110 82 319 600 1251 15 1247 2801 Cuts at: 9416 9912 2991 Size: 496 3292 Fragments arranged by size: 3292 2801 1251 1247 600 496 319 110 82 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5021 1163 Size: 573 3285 6355 Fragments arranged by size: 6355 3285 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2721 6612 7663 8424 9452 1540 Size: 275 186 720 3891 1051 761 1028 2301 Fragments arranged by size: 3891 2301 1051 1028 761 720 275 186 BsiHKAI G_wGCw'C Cuts at: 2027 2388 3672 3774 3819 3834 4415 5094 6210 Size: 361 1284 102 45 15 581 679 1116 Cuts at: 6210 8274 8359 9430 2027 Size: 2064 85 1071 2810 Fragments arranged by size: 2810 2064 1284 1116 1071 679 581 361 102 85 45 15 BsmI GAATG_Cn' Cuts at: 7724 7817 7724 Size: 93 10120 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 2045 2154 2351 2388 2683 2731 Size: 399 237 18 109 197 37 295 48 Cuts at: 2731 3065 3108 3308 3562 3638 3672 3774 3819 Size: 334 43 200 254 76 34 102 45 Cuts at: 3819 3834 4415 4556 4779 5094 5146 5404 5422 Size: 15 581 141 223 315 52 258 18 Cuts at: 5422 6210 6627 6654 6689 6837 7155 7532 8274 Size: 788 417 27 35 148 318 377 742 Cuts at: 8274 8359 9430 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 788 742 581 417 399 377 334 318 315 295 258 254 237 223 200 197 148 141 109 102 85 76 52 48 45 43 37 35 34 27 18 18 15 BspEI T'CCGG_A Cuts at: 6408 9876 6408 Size: 3468 6745 BspHI T'CATG_A Cuts at: 2415 2445 2607 3210 5121 5484 6081 6429 8102 Size: 30 162 603 1911 363 597 348 1673 Cuts at: 8102 9832 2415 Size: 1730 2796 Fragments arranged by size: 2796 1911 1730 1673 603 597 363 348 162 30 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2338 2349 4227 4476 5710 7236 7568 844 Size: 1494 11 1878 249 1234 1526 332 3489 Fragments arranged by size: 3489 1878 1526 1494 1234 332 249 11 BsrBI CCG'CTC Cuts at: 1394 1823 8100 1394 Size: 429 6277 3507 Fragments arranged by size: 6277 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2899 2917 2935 2953 3529 8708 8882 Size: 1183 1651 18 18 18 576 5179 174 Cuts at: 8882 65 Size: 1396 Fragments arranged by size: 5179 1651 1396 1183 576 174 18 18 18 BsrFI r'CCGG_y Cuts at: 1700 1810 4322 5552 6611 7221 8857 9044 1700 Size: 110 2512 1230 1059 610 1636 187 2869 Fragments arranged by size: 2869 2512 1636 1230 1059 610 187 110 BsrGI T'GTAC_A Cuts at: 96 6159 6780 96 Size: 6063 621 3529 Fragments arranged by size: 6063 3529 621 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10213 BssSI C'ACGA_G Cuts at: 6050 7181 7349 8273 9285 6050 Size: 1131 168 924 1012 6978 Fragments arranged by size: 6978 1131 1012 924 168 BstAPI GCAn_nnn'nTGC Cuts at: 794 3519 4278 5073 6585 7483 794 Size: 2725 759 795 1512 898 3524 Fragments arranged by size: 3524 2725 1512 898 795 759 BstBI TT'CG_AA Cuts at: 9017 9017 Size: 10213 BstEII G'GTnAC_C Cuts at: 1184 4106 6106 1184 Size: 2922 2000 5291 Fragments arranged by size: 5291 2922 2000 BstXI CCAn_nnnn'nTGG Cuts at: 2482 3645 5042 5353 6118 6796 7355 2482 Size: 1163 1397 311 765 678 559 5340 Fragments arranged by size: 5340 1397 1163 765 678 559 311 BstYI r'GATC_y Cuts at: 1972 2254 2502 4545 6027 7023 7203 7543 7910 Size: 282 248 2043 1482 996 180 340 367 Cuts at: 7910 8295 8312 9764 9850 9862 10208 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 2043 1977 1482 1452 996 385 367 346 340 282 248 180 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2657 2831 3114 4845 4935 4974 5414 2657 Size: 174 283 1731 90 39 440 7456 Fragments arranged by size: 7456 1731 440 283 174 90 39 BtgI C'CryG_G Cuts at: 513 1065 2004 3336 3345 3508 3603 3641 3692 Size: 552 939 1332 9 163 95 38 51 Cuts at: 3692 3792 3897 4149 5041 5763 6391 7005 7104 Size: 100 105 252 892 722 628 614 99 Cuts at: 7104 513 Size: 3622 Fragments arranged by size: 3622 1332 939 892 722 628 614 552 252 163 105 100 99 95 51 38 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3525 4765 6769 6892 487 Size: 683 56 2299 1240 2004 123 3808 Fragments arranged by size: 3808 2299 2004 1240 683 123 56 BtsI GCAGTG_nn' Cuts at: 3100 3106 5143 5393 7027 7446 7729 8523 8543 Size: 6 2037 250 1634 419 283 794 20 Cuts at: 8543 3100 Size: 4770 Fragments arranged by size: 4770 2037 1634 794 419 283 250 20 6 ClaI AT'CG_AT Cuts at: 1554 6543 1554 Size: 4989 5224 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10178 DraI TTT'AAA Cuts at: 7517 7517 Size: 10213 DraIII CAC_nnn'GTG Cuts at: 2171 2883 3667 2171 Size: 712 784 8717 Fragments arranged by size: 8717 784 712 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2600 6269 9220 809 Size: 507 1284 3669 2951 1802 Fragments arranged by size: 3669 2951 1802 1284 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3333 3342 Size: 54 1425 50 275 18 177 1326 9 Cuts at: 3342 3378 3789 3981 4041 4052 4320 4324 5343 Size: 36 411 192 60 11 268 4 1019 Cuts at: 5343 5724 6609 6813 7345 7660 8549 10178 8 Size: 381 885 204 532 315 889 1629 43 Fragments arranged by size: 1629 1425 1326 1019 889 885 532 411 381 315 275 268 204 192 177 60 54 50 43 36 18 11 9 4 EagI C'GGCC_G Cuts at: 1537 1812 6609 7660 1537 Size: 275 4797 1051 4090 Fragments arranged by size: 4797 4090 1051 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2822 3206 3242 3612 3788 5876 6455 6476 Size: 1469 384 36 370 176 2088 579 21 Cuts at: 6476 8143 1353 Size: 1667 3423 Fragments arranged by size: 3423 2088 1667 1469 579 384 370 176 36 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2311 3157 3194 6344 8800 9174 Size: 1064 138 722 846 37 3150 2456 374 Cuts at: 9174 9320 387 Size: 146 1280 Fragments arranged by size: 3150 2456 1280 1064 846 722 374 146 138 37 EcoICRI GAG'CTC Cuts at: 4413 4413 Size: 10213 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2264 2338 2581 2732 3077 3163 3470 Size: 393 632 74 243 151 345 86 307 Cuts at: 3470 3719 3905 4402 4626 4685 4823 5509 6695 Size: 249 186 497 224 59 138 686 1186 Cuts at: 6695 6818 8276 8873 9660 9928 1239 Size: 123 1458 597 787 268 1524 Fragments arranged by size: 1524 1458 1186 787 686 632 597 497 393 345 307 268 249 243 224 186 151 138 123 86 74 59 EcoNI CCTnn'n_nnAGG Cuts at: 3380 4580 4667 5590 5717 7470 3380 Size: 1200 87 923 127 1753 6123 Fragments arranged by size: 6123 1753 1200 923 127 87 EcoO109I rG'GnC_Cy Cuts at: 2042 2248 2575 2661 2680 2727 2728 3499 4660 Size: 206 327 86 19 47 1 771 1161 Cuts at: 4660 4930 5070 5372 5401 6651 7063 7212 2042 Size: 270 140 302 29 1250 412 149 5043 Fragments arranged by size: 5043 1250 1161 771 412 327 302 270 206 149 140 86 47 29 19 1 EcoRI G'AATT_C Cuts at: 7522 7900 7522 Size: 378 9835 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2296 2326 4222 5296 6082 2296 Size: 30 1896 1074 786 6427 Fragments arranged by size: 6427 1896 1074 786 30 FseI GG_CCGG'CC Cuts at: 4326 4326 Size: 10213 FspI TGC'GCA Cuts at: 5066 8719 5066 Size: 3653 6560 HaeII r_GCGC'y Cuts at: 1678 4103 4425 5008 5472 7240 9360 1678 Size: 2425 322 583 464 1768 2120 2531 Fragments arranged by size: 2531 2425 2120 1768 583 464 322 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2799 2831 4820 4852 4871 Size: 32 135 32 1227 32 1989 32 19 Cuts at: 4871 4903 8854 8886 8928 8960 1373 Size: 32 3951 32 42 32 2626 Fragments arranged by size: 3951 2626 1989 1227 135 42 32 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 2554 5542 5614 5680 6775 7557 Size: 988 281 616 2988 72 66 1095 782 Cuts at: 7557 7803 669 Size: 246 3079 Fragments arranged by size: 3079 2988 1095 988 782 616 281 246 72 66 HindIII A'AGCT_T Cuts at: 748 5978 748 Size: 5230 4983 HpaI GTT'AAC Cuts at: 7803 7803 Size: 10213 KasI G'GCGC_C Cuts at: 5468 5468 Size: 10213 KpnI G_GTAC'C Cuts at: 7538 7538 Size: 10213 MfeI C'AATT_G Cuts at: 7812 7812 Size: 10213 MluI A'CGCG_T Cuts at: 10089 10089 Size: 10213 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2736 3090 3621 3908 4821 5720 6534 9327 Size: 1431 354 531 287 913 899 814 2793 Cuts at: 9327 9511 1305 Size: 184 2007 Fragments arranged by size: 2793 2007 1431 913 899 814 531 354 287 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 3344 3791 3983 4043 Size: 54 1425 343 177 1335 447 192 60 Cuts at: 4043 4054 5345 5726 6815 7347 10 Size: 11 1291 381 1089 532 2876 Fragments arranged by size: 2876 1425 1335 1291 1089 532 447 381 343 192 177 60 54 11 MslI CAynn'nnrTG Cuts at: 518 1166 2020 2030 2381 2450 2609 3643 3791 Size: 648 854 10 351 69 159 1034 148 Cuts at: 3791 4236 4316 4358 4772 5240 5444 5489 5936 Size: 445 80 42 414 468 204 45 447 Cuts at: 5936 6080 6113 6116 6170 7353 8171 8530 8689 Size: 144 33 3 54 1183 818 359 159 Cuts at: 8689 518 Size: 2042 Fragments arranged by size: 2042 1183 1034 854 818 648 468 447 445 414 359 351 204 159 159 148 144 80 69 54 45 42 33 10 3 MspA1I CmG'CkG Cuts at: 1606 2095 2125 3183 3244 3260 3579 3588 4165 Size: 489 30 1058 61 16 319 9 577 Cuts at: 4165 4907 5176 5661 6393 6648 6955 7287 7629 Size: 742 269 485 732 255 307 332 342 Cuts at: 7629 8306 9454 9699 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1058 742 732 677 577 489 485 342 332 319 307 269 255 245 61 30 16 9 NaeI GCC'GGC Cuts at: 4324 5554 6613 7223 4324 Size: 1230 1059 610 7314 Fragments arranged by size: 7314 1230 1059 610 NarI GG'CG_CC Cuts at: 5469 5469 Size: 10213 NcoI C'CATG_G Cuts at: 513 1065 2004 3345 3508 3603 3641 3792 3897 Size: 552 939 1341 163 95 38 151 105 Cuts at: 3897 7005 7104 513 Size: 3108 99 3622 Fragments arranged by size: 3622 3108 1341 939 552 163 151 105 99 95 38 NdeI CA'TA_TG Cuts at: 387 3352 3674 387 Size: 2965 322 6926 Fragments arranged by size: 6926 2965 322 NgoMIV G'CCGG_C Cuts at: 4322 5552 6611 7221 4322 Size: 1230 1059 610 7314 Fragments arranged by size: 7314 1230 1059 610 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10213 NotI GC'GGCC_GC Cuts at: 7660 7660 Size: 10213 NsiI A_TGCA'T Cuts at: 4239 4239 Size: 10213 NspI r_CATG'y Cuts at: 1132 1229 2410 4069 4162 4237 5818 6508 6781 Size: 97 1181 1659 93 75 1581 690 273 Cuts at: 6781 7264 7571 9116 1132 Size: 483 307 1545 2229 Fragments arranged by size: 2229 1659 1581 1545 1181 690 483 307 273 97 93 75 PasI CC'CwG_GG Cuts at: 1348 2705 5035 7472 1348 Size: 1357 2330 2437 4089 Fragments arranged by size: 4089 2437 2330 1357 PciI A'CATG_T Cuts at: 5814 6777 7260 9112 5814 Size: 963 483 1852 6915 Fragments arranged by size: 6915 1852 963 483 PflMI CCAn_nnn'nTGG Cuts at: 1108 3475 3509 4050 5292 6037 7033 1108 Size: 2367 34 541 1242 745 996 4288 Fragments arranged by size: 4288 2367 1242 996 745 541 34 PfoI T'CCnGG_A Cuts at: 2571 3054 6795 2571 Size: 483 3741 5989 Fragments arranged by size: 5989 3741 483 PmeI GTTT'AAAC Cuts at: 7517 7517 Size: 10213 PmlI CAC'GTG Cuts at: 2883 2901 2973 2883 Size: 18 72 10123 Fragments arranged by size: 10123 72 18 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7100 7132 7352 7384 8282 8314 9795 9827 7100 Size: 32 220 32 898 32 1481 32 7486 Fragments arranged by size: 7486 1481 898 220 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2248 2575 2661 3499 4660 4930 5372 7063 2248 Size: 327 86 838 1161 270 442 1691 5398 Fragments arranged by size: 5398 1691 1161 838 442 327 270 86 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10213 PsiI TTA'TAA Cuts at: 7783 7783 Size: 10213 PspOMI G'GGCC_C Cuts at: 2041 2679 2727 5400 6650 2041 Size: 638 48 2673 1250 5604 Fragments arranged by size: 5604 2673 1250 638 48 PspXI vC'TCGA_Gb Cuts at: 4724 4724 Size: 10213 PstI C_TGCA'G Cuts at: 830 2124 2814 4473 4901 5721 6743 6948 7565 Size: 1294 690 1659 428 820 1022 205 617 Cuts at: 7565 830 Size: 3478 Fragments arranged by size: 3478 1659 1294 1022 820 690 617 428 205 PvuI CG_AT'CG Cuts at: 2001 2721 2001 Size: 720 9493 PvuII CAG'CTG Cuts at: 3183 3244 3260 4165 4907 3183 Size: 61 16 905 742 8489 Fragments arranged by size: 8489 905 742 61 16 SacI G_AGCT'C Cuts at: 4415 4415 Size: 10213 SacII CC_GC'GG Cuts at: 6394 6394 Size: 10213 SalI G'TCGA_C Cuts at: 1936 2552 7555 1936 Size: 616 5003 4594 Fragments arranged by size: 5003 4594 616 SanDI GG'GwC_CC Cuts at: 2575 3499 5372 2575 Size: 924 1873 7416 Fragments arranged by size: 7416 1873 924 SapI GCTCTTCn'nnn_ Cuts at: 2822 2822 Size: 10213 SbfI CC_TGCA'GG Cuts at: 5721 6948 7565 5721 Size: 1227 617 8369 Fragments arranged by size: 8369 1227 617 ScaI AGT'ACT Cuts at: 6233 6323 8461 10173 6233 Size: 90 2138 1712 6273 Fragments arranged by size: 6273 2138 1712 90 SexAI A'CCwGG_T Cuts at: 3846 3846 Size: 10213 SfcI C'TryA_G Cuts at: 826 1045 2120 2263 2810 4469 4897 5717 6739 Size: 219 1075 143 547 1659 428 820 1022 Cuts at: 6739 6913 6944 7232 7561 8696 9377 9568 826 Size: 174 31 288 329 1135 681 191 1471 Fragments arranged by size: 1659 1471 1135 1075 1022 820 681 547 428 329 288 219 191 174 143 31 SfiI GGCCn_nnn'nGGCC Cuts at: 3341 3377 3341 Size: 36 10177 SfoI GGC'GCC Cuts at: 5470 5470 Size: 10213 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10213 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10213 SmaI CCC'GGG Cuts at: 1872 2069 5774 6684 7540 1872 Size: 197 3705 910 856 4545 Fragments arranged by size: 4545 3705 910 856 197 SmlI C'TyrA_G Cuts at: 820 1017 1943 3419 4724 6143 6209 7094 7169 Size: 197 926 1476 1305 1419 66 885 75 Cuts at: 7169 8317 9218 9480 820 Size: 1148 901 262 1553 Fragments arranged by size: 1553 1476 1419 1305 1148 926 901 885 262 197 75 66 SnaBI TAC'GTA Cuts at: 493 8004 493 Size: 7511 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10213 SphI G_CATG'C Cuts at: 1132 4162 7571 1132 Size: 3030 3409 3774 Fragments arranged by size: 3774 3409 3030 SspI AAT'ATT Cuts at: 5 52 8137 5 Size: 47 8085 2081 Fragments arranged by size: 8085 2081 47 StuI AGG'CCT Cuts at: 1517 3978 4517 4994 6065 1517 Size: 2461 539 477 1071 5665 Fragments arranged by size: 5665 2461 1071 539 477 StyI C'CwwG_G Cuts at: 513 1065 2004 3345 3459 3508 3603 3641 3792 Size: 552 939 1341 114 49 95 38 151 Cuts at: 3792 3897 4206 4512 4557 5464 6399 6838 7005 Size: 105 309 306 45 907 935 439 167 Cuts at: 7005 7104 7137 7652 513 Size: 99 33 515 3074 Fragments arranged by size: 3074 1341 939 935 907 552 515 439 309 306 167 151 114 105 99 95 49 45 38 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5245 5525 6349 6914 7058 8240 8257 Size: 1255 3665 280 824 565 144 1182 17 Cuts at: 8257 8410 8595 9040 325 Size: 153 185 445 1498 Fragments arranged by size: 3665 1498 1255 1182 824 565 445 280 185 153 144 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2487 5277 6159 Size: 274 80 33 51 1443 510 2790 882 Cuts at: 6159 6231 6321 6780 8459 10171 96 Size: 72 90 459 1679 1712 138 Fragments arranged by size: 2790 1712 1679 1443 882 510 459 274 138 90 80 72 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3079 3111 3987 4019 4178 4210 6358 6390 10173 Size: 32 876 32 159 32 2148 32 3783 Cuts at: 10173 10205 3079 Size: 32 3087 Fragments arranged by size: 3783 3087 2148 876 159 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5213 1316 Size: 3897 6316 XbaI T'CTAG_A Cuts at: 7549 7549 Size: 10213 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3610 4726 5335 7063 3610 Size: 1116 609 1728 6760 Fragments arranged by size: 6760 1728 1116 609 XhoI C'TCGA_G Cuts at: 1943 4724 1943 Size: 2781 7432 XmaI C'CCGG_G Cuts at: 1870 2067 5772 6682 7538 1870 Size: 197 3705 910 856 4545 Fragments arranged by size: 4545 3705 910 856 197 XmnI GAAnn'nnTTC Cuts at: 2279 3236 3935 4391 6323 8342 2279 Size: 957 699 456 1932 2019 4150 Fragments arranged by size: 4150 2019 1932 957 699 456 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8021 276 Size: 53 83 186 936 6487 2468 Fragments arranged by size: 6487 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BplI BsiWI BsmBI BstZ17I EcoRV FspAI NruI PacI PsrI RsrII SrfI SwaI