################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BbrPI BcgI BciVI BclI BfrI BlnI BsaAI BseAI BsiEI BsiWI BsmI Bsp1407I BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I DraI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI HpaI KpnI MfeI MluI MluNI MroI MscI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SacI SapI SbfI ScaI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 1183 GTCGAC GT^[AC][GT]AC AcsI 1580 GAATTC [GA]^AATT[CT] AcyI 55 GGCGCC G[GA]^CG[CT]C AocI 1465 CCTGAGG CC^T[ACGT]AGG ApoI 1580 GAATTC [GA]^AATT[CT] AspI 131 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 160 CCCGGG C^[CT]CG[GA]G BamHI 336 GGATCC G^GATCC BbeI 55 GGCGCC GGCGC^C BbvCI 436 CCTCAGC CCTCAGC(-5/-2) BfaI 725 CTAG C^TAG BglII 1082 AGATCT A^GATCT BlpI 39 GCTAAGC GC^T[ACGT]AGC BpmI 872 CTGGAG CTGGAG(16/14) Bpu1102I 39 GCTAAGC GC^T[ACGT]AGC BsaBI 531 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 55 GGCGCC G[GA]^CG[CT]C BsmBI 1476 GAGACG CGTCTC(1/5) BsoBI 160 CCCGGG C^[CT]CG[GA]G BspDI 1222 ATCGAT AT^CGAT BspMI 261 GCAGGT ACCTGC(4/8) BsrDI 516 CATTGC GCAATG(2/0) BsrFI 117 ACCGGC [GA]^CCGG[CT] BssSI 1042 CACGAG CACGAG(-5/-1) Bsu36I 1465 CCTGAGG CC^T[ACGT]AGG CelII 39 GCTAAGC GC^T[ACGT]AGC Cfr10I 117 ACCGGC [GA]^CCGG[CT] ClaI 1222 ATCGAT AT^CGAT CpoI 114 CGGACCG CG^G[AT]CCG DraIII 808 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1095 TGGCCG [CT]^GGCC[GA] EarI 735 GAAGAG CTCTTC(1/4) Eco81I 1465 CCTGAGG CC^T[ACGT]AGG EcoRI 1580 GAATTC G^AATTC Hin1I 55 GGCGCC G[GA]^CG[CT]C HincII 1183 GTCGAC GT[CT]^[GA]AC HindII 1183 GTCGAC GT[CT]^[GA]AC HindIII 577 AAGCTT A^AGCTT KasI 55 GGCGCC G^GCGCC Ksp632I 735 GAAGAG CTCTTC(1/4) MaeI 725 CTAG C^TAG MamI 531 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 55 GGCGCC GG^CGCC PflFI 131 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC RsrII 114 CGGACCG CG^G[AT]CCG SalI 1183 GTCGAC G^TCGAC SexAI 809 ACCTGGT A^CC[AT]GGT SfiI 187 GGCCCCGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 55 GGCGCC GGC^GCC SmaI 160 CCCGGG CCC^GGG SmlI 1435 CTCAAG C^T[CT][GA]AG Tsp45I 228 GTCAC ^GT[GC]AC Tth111I 131 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 160 CCCGGG C^CCGGG XmaI 160 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Alw44I 891 GTGCAC G^TGCAC 967 GTGCAC ApaLI 891 GTGCAC G^TGCAC 967 GTGCAC AspHI 891 GTGCAC G[AT]GC[AT]^C 967 GTGCAC BanI 55 GGCGCC G^G[CT][GA]CC 821 GGCACC BbsI 1203 GAAGAC GAAGAC(2/6) 1350 GAAGAC BpuAI 1203 GAAGAC GAAGAC(2/6) 1350 GAAGAC BsaWI 239 ACCGGA [AT]^CCGG[AT] 824 ACCGGA BsiHKAI 891 GTGCAC G[AT]GC[AT]^C 967 GTGCAC BsrBI 496 GAGCGG CCGCTC(-3/-3) 1045 GAGCGG BsrI 1563 ACTGG ACTGG(1/-1) 927 CCAGT BstAPI 634 GCAGGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 822 GCACCGGATGC HphI 229 TCACC GGTGA(8/7) 807 TCACC MaeII 601 ACGT A^CGT 884 ACGT MseI 403 TTAA T^TAA 758 TTAA NcoI 182 CCATGG C^CATGG 248 CCATGG PvuII 1027 CAGCTG CAG^CTG 1345 CAGCTG SphI 104 GCATGC GCATG^C 317 GCATGC TfiI 443 GAATC G^A[AT]TC 1116 GATTC Tru9I 403 TTAA T^TAA 758 TTAA TspRI 1244 CACTG CA[GC]TG[ACGT][ACGT]^ 1446 CACTG XcmI 840 CCAGGAGACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1026 CCAGCTGCAGGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI ApaI AvaII BanII BbvI BcnI BglI BmyI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DrdI DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI KspI MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp509I TthHB8I XhoII