(Circular) (Six-base) MAPSORT of: pFN21AB5820.seq Check: 728 from: 1 to: 6298 pFN21AB5820 6298 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2257 2257 Size: 6298 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4108 278 Size: 53 83 186 936 2572 2468 Fragments arranged by size: 2572 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3189 3641 1937 Size: 1252 452 4594 Fragments arranged by size: 4594 1252 452 Acc65I G'GTAC_C Cuts at: 3619 3619 Size: 6298 AclI AA'CG_TT Cuts at: 1563 4425 4798 1563 Size: 2862 373 3063 Fragments arranged by size: 3063 2862 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3076 3272 3374 4361 5745 6013 1632 Size: 1444 196 102 987 1384 268 1917 Fragments arranged by size: 1917 1444 1384 987 268 196 102 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6101 AflIII A'CryG_T Cuts at: 1203 5197 6174 1203 Size: 3994 977 1327 Fragments arranged by size: 3994 1327 977 AgeI A'CCGG_T Cuts at: 5129 5129 Size: 6298 AhdI GACnn_n'nnGTC Cuts at: 5027 5027 Size: 6298 AleI CACnn'nnGTG Cuts at: 1166 3455 1166 Size: 2289 4009 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2921 3555 1184 Size: 1737 634 3927 Fragments arranged by size: 3927 1737 634 AlwNI CAG_nnn'CTG Cuts at: 2645 3007 3568 3580 4041 5613 2645 Size: 362 561 12 461 1572 3330 Fragments arranged by size: 3330 1572 561 461 362 12 ApaI G_GGCC'C Cuts at: 2165 2195 2204 3514 2165 Size: 30 9 1310 4949 Fragments arranged by size: 4949 1310 30 9 ApaLI G'TGCA_C Cuts at: 2896 2972 4355 5511 2896 Size: 76 1383 1156 3683 Fragments arranged by size: 3683 1383 1156 76 ApoI r'AATT_y Cuts at: 1493 3585 3607 3833 3985 6094 1493 Size: 2092 22 226 152 2109 1697 Fragments arranged by size: 2109 2092 1697 226 152 22 AseI AT'TA_AT Cuts at: 160 1032 4852 160 Size: 872 3820 1606 Fragments arranged by size: 3820 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 AvaI C'yCGr_G Cuts at: 1870 1943 2165 3623 1870 Size: 73 222 1458 4545 Fragments arranged by size: 4545 1458 222 73 BamHI G'GATC_C Cuts at: 2341 3628 3995 2341 Size: 1287 367 4644 Fragments arranged by size: 4644 1287 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2826 3619 5074 618 Size: 325 217 627 273 766 793 1455 1842 Fragments arranged by size: 1842 1455 793 766 627 325 273 217 BanII G_rGCy'C Cuts at: 1391 2165 2195 2204 3514 3617 1391 Size: 774 30 9 1310 103 4072 Fragments arranged by size: 4072 1310 774 103 30 9 BbeI G_GCGC'C Cuts at: 2064 2064 Size: 6298 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3215 3362 928 Size: 971 1316 147 3864 Fragments arranged by size: 3864 1316 971 147 BbvCI CC'TCA_GC Cuts at: 2442 2442 Size: 6298 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4489 4523 1903 Size: 34 2552 34 3678 Fragments arranged by size: 3678 2552 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4190 5400 4190 Size: 1210 5088 BclI T'GATC_A Cuts at: 1548 1548 Size: 6298 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2055 2190 2199 Size: 107 122 71 631 605 383 135 9 Cuts at: 2199 4909 136 Size: 2710 1525 Fragments arranged by size: 2710 1525 631 605 383 135 122 107 71 9 BglII A'GATC_T Cuts at: 3087 6293 3087 Size: 3206 3092 BlpI GC'TnA_GC Cuts at: 2045 3715 2045 Size: 1670 4628 Bme1580I G_kGCm'C Cuts at: 1790 2165 2195 2204 2370 2900 2976 3048 3514 Size: 375 30 9 166 530 76 72 466 Cuts at: 3514 4359 5515 1790 Size: 845 1156 2573 Fragments arranged by size: 2573 1156 845 530 466 375 166 76 72 30 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6298 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2926 4029 4987 Size: 416 53 83 399 24 1509 1103 958 Cuts at: 4987 442 Size: 1753 Fragments arranged by size: 1753 1509 1103 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6298 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3322 3322 Size: 6298 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2898 4958 1239 Size: 1659 2060 2579 Fragments arranged by size: 2579 2060 1659 Bpu10I CC'TnA_GC Cuts at: 2442 2442 Size: 6298 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3425 4423 5288 5586 3425 Size: 998 865 298 4137 Fragments arranged by size: 4137 998 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2874 3323 3429 4961 882 Size: 617 1375 449 106 1532 2219 Fragments arranged by size: 2219 1532 1375 617 449 106 BsaAI yAC'GTr Cuts at: 493 1204 4089 493 Size: 711 2885 2702 Fragments arranged by size: 2885 2702 711 BsaBI GATnn'nnATC Cuts at: 2540 6292 2540 Size: 3752 2546 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2061 4105 4487 5122 Size: 53 83 186 936 528 2044 382 635 Cuts at: 5122 275 Size: 1451 Fragments arranged by size: 2044 1451 936 635 528 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2244 2829 4731 5129 5403 5550 5961 2244 Size: 585 1902 398 274 147 411 2581 Fragments arranged by size: 2581 1902 585 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2112 2142 1178 Size: 30 165 30 709 30 5334 Fragments arranged by size: 5334 709 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2428 3488 3491 1186 Size: 1242 1060 3 3993 Fragments arranged by size: 3993 1242 1060 3 BseYI C'CCAG_C Cuts at: 2394 2615 2668 2965 3426 3580 5501 5997 2394 Size: 221 53 297 461 154 1921 496 2695 Fragments arranged by size: 2695 1921 496 461 297 221 154 53 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2319 2658 3569 1163 Size: 573 583 339 911 3892 Fragments arranged by size: 3892 911 583 573 339 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3748 4509 5537 1540 Size: 275 186 1747 761 1028 2301 Fragments arranged by size: 2301 1747 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2900 2976 4359 4444 5515 2900 Size: 76 1383 85 1071 3683 Fragments arranged by size: 3683 1383 1071 85 76 BsmI GAATG_Cn' Cuts at: 3809 3902 3809 Size: 93 6205 BsmBI CGTCTCn'nnnn_ Cuts at: 3475 3475 Size: 6298 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2165 2195 2204 2370 2900 2976 3048 Size: 399 375 30 9 166 530 76 72 Cuts at: 3048 3514 3617 4359 4444 5515 1391 Size: 466 103 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 530 466 399 375 166 103 85 76 72 30 9 BspEI T'CCGG_A Cuts at: 5961 5961 Size: 6298 BspHI T'CATG_A Cuts at: 4187 5917 4187 Size: 1730 4568 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2257 3653 844 Size: 1413 1396 3489 Fragments arranged by size: 3489 1413 1396 BsrBI CCG'CTC Cuts at: 1394 1823 2503 3052 4185 1394 Size: 429 680 549 1133 3507 Fragments arranged by size: 3507 1133 680 549 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2520 4793 4967 65 Size: 1183 1272 2273 174 1396 Fragments arranged by size: 2273 1396 1272 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2122 4942 5129 1700 Size: 110 312 2820 187 2869 Fragments arranged by size: 2869 2820 312 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6298 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6298 BssSI C'ACGA_G Cuts at: 3047 4358 5370 3047 Size: 1311 1012 3975 Fragments arranged by size: 3975 1311 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2645 2833 794 Size: 1851 188 4259 Fragments arranged by size: 4259 1851 188 BstBI TT'CG_AA Cuts at: 5102 5102 Size: 6298 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6298 BstXI CCAn_nnnn'nTGG Cuts at: 2225 2338 3133 2225 Size: 113 795 5390 Fragments arranged by size: 5390 795 113 BstYI r'GATC_y Cuts at: 1972 2341 2485 2541 2589 3087 3321 3628 3995 Size: 369 144 56 48 498 234 307 367 Cuts at: 3995 4380 4397 5849 5935 5947 6293 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 498 385 369 367 346 307 234 144 86 56 48 17 12 Bsu36I CC'TnA_GG Cuts at: 3471 3471 Size: 6298 BtgI C'CryG_G Cuts at: 513 1065 2064 2097 2187 2196 2205 2253 513 Size: 552 999 33 90 9 9 48 4558 Fragments arranged by size: 4558 999 552 90 48 33 9 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5559 Fragments arranged by size: 5559 683 56 BtsI GCAGTG_nn' Cuts at: 3814 4608 4628 3814 Size: 794 20 5484 Fragments arranged by size: 5484 794 20 ClaI AT'CG_AT Cuts at: 1554 3228 1554 Size: 1674 4624 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6263 DraI TTT'AAA Cuts at: 3602 3602 Size: 6298 DraIII CAC_nnn'GTG Cuts at: 2818 2818 Size: 6298 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2888 3164 3337 5305 809 Size: 507 1572 276 173 1968 1802 Fragments arranged by size: 1968 1802 1572 507 276 173 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3100 3745 4634 Size: 54 1425 50 275 18 1270 645 889 Cuts at: 4634 6263 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1270 889 645 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3745 1537 Size: 275 1933 4090 Fragments arranged by size: 4090 1933 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2735 4228 1353 Size: 1382 1493 3423 Fragments arranged by size: 3423 1493 1382 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2171 2639 3071 4885 5259 5405 Size: 1064 138 582 468 432 1814 374 146 Cuts at: 5405 387 Size: 1280 Fragments arranged by size: 1814 1280 1064 582 468 432 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2898 3076 3272 3374 4361 4958 5745 Size: 393 1266 178 196 102 987 597 787 Cuts at: 5745 6013 1239 Size: 268 1524 Fragments arranged by size: 1524 1266 987 787 597 393 268 196 178 102 EcoO109I rG'GnC_Cy Cuts at: 2102 2161 2162 2192 2201 2273 2652 3004 2102 Size: 59 1 30 9 72 379 352 5396 Fragments arranged by size: 5396 379 352 72 59 30 9 1 EcoRI G'AATT_C Cuts at: 3585 3607 3985 3585 Size: 22 378 5898 Fragments arranged by size: 5898 378 22 FspI TGC'GCA Cuts at: 4804 4804 Size: 6298 HaeII r_GCGC'y Cuts at: 1678 2064 2087 2129 2401 3433 5445 1678 Size: 386 23 42 272 1032 2012 2531 Fragments arranged by size: 2531 2012 1032 386 272 42 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2112 2144 2509 2541 3451 Size: 32 135 32 540 32 365 32 910 Cuts at: 3451 3483 4939 4971 5013 5045 1373 Size: 32 1456 32 42 32 2626 Fragments arranged by size: 2626 1456 910 540 365 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3190 3642 3888 669 Size: 988 281 1252 452 246 3079 Fragments arranged by size: 3079 1252 988 452 281 246 HindIII A'AGCT_T Cuts at: 748 2582 748 Size: 1834 4464 HpaI GTT'AAC Cuts at: 3888 3888 Size: 6298 KasI G'GCGC_C Cuts at: 2060 2060 Size: 6298 KpnI G_GTAC'C Cuts at: 3623 3623 Size: 6298 MfeI C'AATT_G Cuts at: 3897 3897 Size: 6298 MluI A'CGCG_T Cuts at: 6174 6174 Size: 6298 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5412 5596 1305 Size: 4107 184 2007 Fragments arranged by size: 4107 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4476 Fragments arranged by size: 4476 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2336 3230 3455 4256 4615 4774 518 Size: 648 1170 894 225 801 359 159 2042 Fragments arranged by size: 2042 1170 894 801 648 359 225 159 MspA1I CmG'CkG Cuts at: 1606 2035 2099 2198 2207 3034 3352 3714 4391 Size: 429 64 99 9 827 318 362 677 Cuts at: 4391 5539 5784 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 827 677 429 362 318 245 99 64 9 NarI GG'CG_CC Cuts at: 2061 2061 Size: 6298 NcoI C'CATG_G Cuts at: 513 1065 2187 2253 513 Size: 552 1122 66 4558 Fragments arranged by size: 4558 1122 552 66 NdeI CA'TA_TG Cuts at: 387 387 Size: 6298 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6298 NotI GC'GGCC_GC Cuts at: 3745 3745 Size: 6298 NspI r_CATG'y Cuts at: 1132 1229 2113 2233 2326 3656 5201 1132 Size: 97 884 120 93 1330 1545 2229 Fragments arranged by size: 2229 1545 1330 884 120 97 93 PasI CC'CwG_GG Cuts at: 1348 2077 2657 3507 1348 Size: 729 580 850 4139 Fragments arranged by size: 4139 850 729 580 PciI A'CATG_T Cuts at: 5197 5197 Size: 6298 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6298 PfoI T'CCnGG_A Cuts at: 2451 2544 3408 2451 Size: 93 864 5341 Fragments arranged by size: 5341 864 93 PmeI GTTT'AAAC Cuts at: 3602 3602 Size: 6298 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4367 4399 5880 5912 4367 Size: 32 1481 32 4753 Fragments arranged by size: 4753 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2102 2273 2652 2102 Size: 171 379 5748 Fragments arranged by size: 5748 379 171 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6298 PsiI TTA'TAA Cuts at: 3868 3868 Size: 6298 PspOMI G'GGCC_C Cuts at: 2161 2191 2200 3510 2161 Size: 30 9 1310 4949 Fragments arranged by size: 4949 1310 30 9 PstI C_TGCA'G Cuts at: 830 2650 3039 3150 3650 830 Size: 1820 389 111 500 3478 Fragments arranged by size: 3478 1820 500 389 111 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6298 PvuII CAG'CTG Cuts at: 3034 3352 3034 Size: 318 5980 RsrII CG'GwC_CG Cuts at: 2120 2120 Size: 6298 SacII CC_GC'GG Cuts at: 2100 2199 2208 2100 Size: 99 9 6190 Fragments arranged by size: 6190 99 9 SalI G'TCGA_C Cuts at: 1936 3188 3640 1936 Size: 1252 452 4594 Fragments arranged by size: 4594 1252 452 SanDI GG'GwC_CC Cuts at: 2102 2273 2652 2102 Size: 171 379 5748 Fragments arranged by size: 5748 379 171 SbfI CC_TGCA'GG Cuts at: 3650 3650 Size: 6298 ScaI AGT'ACT Cuts at: 4546 6258 4546 Size: 1712 4586 SexAI A'CCwGG_T Cuts at: 2814 2814 Size: 6298 SfcI C'TryA_G Cuts at: 826 1045 2646 3035 3146 3646 4781 5462 5653 Size: 219 1601 389 111 500 1135 681 191 Cuts at: 5653 826 Size: 1471 Fragments arranged by size: 1601 1471 1135 681 500 389 219 191 111 SfiI GGCCn_nnn'nGGCC Cuts at: 2199 2199 Size: 6298 SfoI GGC'GCC Cuts at: 2062 2062 Size: 6298 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6298 SmaI CCC'GGG Cuts at: 1872 2167 3625 1872 Size: 295 1458 4545 Fragments arranged by size: 4545 1458 295 SmlI C'TyrA_G Cuts at: 820 1017 1943 3440 4402 5303 5565 820 Size: 197 926 1497 962 901 262 1553 Fragments arranged by size: 1553 1497 962 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4089 493 Size: 3596 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6298 SphI G_CATG'C Cuts at: 1132 2113 2326 3656 1132 Size: 981 213 1330 3774 Fragments arranged by size: 3774 1330 981 213 SspI AAT'ATT Cuts at: 5 52 4222 5 Size: 47 4170 2081 Fragments arranged by size: 4170 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6298 StyI C'CwwG_G Cuts at: 513 1065 2187 2253 3543 3737 513 Size: 552 1122 66 1290 194 3074 Fragments arranged by size: 3074 1290 1122 552 194 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2344 2858 4325 4342 4495 4680 5125 Size: 1255 764 514 1467 17 153 185 445 Cuts at: 5125 325 Size: 1498 Fragments arranged by size: 1498 1467 1255 764 514 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2934 3057 4544 Size: 274 80 33 51 1443 957 123 1487 Cuts at: 4544 6256 96 Size: 1712 138 Fragments arranged by size: 1712 1487 1443 957 274 138 123 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6258 6290 6258 Size: 32 6266 Tth111I GACn'n_nGTC Cuts at: 1316 2139 1316 Size: 823 5475 XbaI T'CTAG_A Cuts at: 3634 3634 Size: 6298 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2852 3038 2852 Size: 186 6112 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6298 XmaI C'CCGG_G Cuts at: 1870 2165 3623 1870 Size: 295 1458 4545 Fragments arranged by size: 4545 1458 295 XmnI GAAnn'nnTTC Cuts at: 4427 4427 Size: 6298 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4106 276 Size: 53 83 186 936 2572 2468 Fragments arranged by size: 2572 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI SacI SapI SrfI SwaI