(Linear) (Six-base) MAPSORT of: KIBB5820.seq Check: 7201 from: 1 to: 1593 KIBB5820.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 252 1593 Size: 252 1341 AccI GT'mk_AC Cuts at: 0 1184 1593 Size: 1184 409 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1071 1267 1369 1593 Size: 1071 196 102 224 Fragments arranged by size: 1071 224 196 102 AleI CACnn'nnGTG Cuts at: 0 1450 1593 Size: 1450 143 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 916 1550 1593 Size: 916 634 43 Fragments arranged by size: 916 634 43 AlwNI CAG_nnn'CTG Cuts at: 0 640 1002 1563 1575 1593 Size: 640 362 561 12 18 Fragments arranged by size: 640 561 362 18 12 ApaI G_GGCC'C Cuts at: 0 160 190 199 1509 1593 Size: 160 30 9 1310 84 Fragments arranged by size: 1310 160 84 30 9 ApaLI G'TGCA_C Cuts at: 0 891 967 1593 Size: 891 76 626 Fragments arranged by size: 891 626 76 ApoI r'AATT_y Cuts at: 0 1580 1593 Size: 1580 13 AvaI C'yCGr_G Cuts at: 0 160 1593 Size: 160 1433 BamHI G'GATC_C Cuts at: 0 336 1593 Size: 336 1257 BanI G'GyrC_C Cuts at: 0 55 821 1593 Size: 55 766 772 Fragments arranged by size: 772 766 55 BanII G_rGCy'C Cuts at: 0 160 190 199 1509 1593 Size: 160 30 9 1310 84 Fragments arranged by size: 1310 160 84 30 9 BbeI G_GCGC'C Cuts at: 0 59 1593 Size: 59 1534 BbsI GAAGACnn'nnnn_ Cuts at: 0 1210 1357 1593 Size: 1210 147 236 Fragments arranged by size: 1210 236 147 BbvCI CC'TCA_GC Cuts at: 0 437 1593 Size: 437 1156 BglI GCCn_nnn'nGGC Cuts at: 0 50 185 194 1593 Size: 50 135 9 1399 Fragments arranged by size: 1399 135 50 9 BglII A'GATC_T Cuts at: 0 1082 1593 Size: 1082 511 BlpI GC'TnA_GC Cuts at: 0 40 1593 Size: 40 1553 Bme1580I G_kGCm'C Cuts at: 0 160 190 199 365 895 971 1043 1509 Size: 160 30 9 166 530 76 72 466 Cuts at: 1509 1593 Size: 84 Fragments arranged by size: 530 466 166 160 84 76 72 30 9 BmrI ACTGGGnnnn_n' Cuts at: 0 921 1593 Size: 921 672 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1317 1593 Size: 1317 276 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 893 1593 Size: 893 700 Bpu10I CC'TnA_GC Cuts at: 0 437 1593 Size: 437 1156 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1420 1593 Size: 1420 173 BsaI GGTCTCn'nnnn_ Cuts at: 0 869 1318 1424 1593 Size: 869 449 106 169 Fragments arranged by size: 869 449 169 106 BsaBI GATnn'nnATC Cuts at: 0 535 1593 Size: 535 1058 BsaHI Gr'CG_yC Cuts at: 0 56 1593 Size: 56 1537 BsaWI w'CCGG_w Cuts at: 0 239 824 1593 Size: 239 585 769 Fragments arranged by size: 769 585 239 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 107 137 1593 Size: 107 30 1456 Fragments arranged by size: 1456 107 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 423 1483 1486 1593 Size: 423 1060 3 107 Fragments arranged by size: 1060 423 107 3 BseYI C'CCAG_C Cuts at: 0 389 610 663 960 1421 1575 1593 Size: 389 221 53 297 461 154 18 Fragments arranged by size: 461 389 297 221 154 53 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 314 653 1564 1593 Size: 314 339 911 29 Fragments arranged by size: 911 339 314 29 BsiHKAI G_wGCw'C Cuts at: 0 895 971 1593 Size: 895 76 622 Fragments arranged by size: 895 622 76 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1470 1593 Size: 1470 123 Bsp1286I G_dGCh'C Cuts at: 0 160 190 199 365 895 971 1043 1509 Size: 160 30 9 166 530 76 72 466 Cuts at: 1509 1593 Size: 84 Fragments arranged by size: 530 466 166 160 84 76 72 30 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 252 1593 Size: 252 1341 BsrBI CCG'CTC Cuts at: 0 498 1047 1593 Size: 498 549 546 Fragments arranged by size: 549 546 498 BsrDI GCAATG_nn' Cuts at: 0 515 1593 Size: 515 1078 BsrFI r'CCGG_y Cuts at: 0 117 1593 Size: 117 1476 BssSI C'ACGA_G Cuts at: 0 1042 1593 Size: 1042 551 BstAPI GCAn_nnn'nTGC Cuts at: 0 640 828 1593 Size: 640 188 765 Fragments arranged by size: 765 640 188 BstXI CCAn_nnnn'nTGG Cuts at: 0 220 333 1128 1593 Size: 220 113 795 465 Fragments arranged by size: 795 465 220 113 BstYI r'GATC_y Cuts at: 0 336 480 536 584 1082 1316 1593 Size: 336 144 56 48 498 234 277 Fragments arranged by size: 498 336 277 234 144 56 48 Bsu36I CC'TnA_GG Cuts at: 0 1466 1593 Size: 1466 127 BtgI C'CryG_G Cuts at: 0 59 92 182 191 200 248 1593 Size: 59 33 90 9 9 48 1345 Fragments arranged by size: 1345 90 59 48 33 9 9 ClaI AT'CG_AT Cuts at: 0 1223 1593 Size: 1223 370 DraIII CAC_nnn'GTG Cuts at: 0 813 1593 Size: 813 780 DrdI GACnn_nn'nnGTC Cuts at: 0 883 1159 1332 1593 Size: 883 276 173 261 Fragments arranged by size: 883 276 261 173 EaeI y'GGCC_r Cuts at: 0 1095 1593 Size: 1095 498 EarI CTCTTCn'nnn_ Cuts at: 0 730 1593 Size: 730 863 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 166 634 1066 1593 Size: 166 468 432 527 Fragments arranged by size: 527 468 432 166 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 893 1071 1267 1369 1593 Size: 893 178 196 102 224 Fragments arranged by size: 893 224 196 178 102 EcoO109I rG'GnC_Cy Cuts at: 0 97 156 157 187 196 268 647 999 Size: 97 59 1 30 9 72 379 352 Cuts at: 999 1593 Size: 594 Fragments arranged by size: 594 379 352 97 72 59 30 9 1 EcoRI G'AATT_C Cuts at: 0 1580 1593 Size: 1580 13 HaeII r_GCGC'y Cuts at: 0 59 82 124 396 1428 1593 Size: 59 23 42 272 1032 165 Fragments arranged by size: 1032 272 165 59 42 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 107 139 504 536 1446 1478 1593 Size: 107 32 365 32 910 32 115 Fragments arranged by size: 910 365 115 107 32 32 32 HincII GTy'rAC Cuts at: 0 1185 1593 Size: 1185 408 HindIII A'AGCT_T Cuts at: 0 577 1593 Size: 577 1016 KasI G'GCGC_C Cuts at: 0 55 1593 Size: 55 1538 MslI CAynn'nnrTG Cuts at: 0 331 1225 1450 1593 Size: 331 894 225 143 Fragments arranged by size: 894 331 225 143 MspA1I CmG'CkG Cuts at: 0 30 94 193 202 1029 1347 1593 Size: 30 64 99 9 827 318 246 Fragments arranged by size: 827 318 246 99 64 30 9 NarI GG'CG_CC Cuts at: 0 56 1593 Size: 56 1537 NcoI C'CATG_G Cuts at: 0 182 248 1593 Size: 182 66 1345 Fragments arranged by size: 1345 182 66 NspI r_CATG'y Cuts at: 0 108 228 321 1593 Size: 108 120 93 1272 Fragments arranged by size: 1272 120 108 93 PasI CC'CwG_GG Cuts at: 0 72 652 1502 1593 Size: 72 580 850 91 Fragments arranged by size: 850 580 91 72 PfoI T'CCnGG_A Cuts at: 0 446 539 1403 1593 Size: 446 93 864 190 Fragments arranged by size: 864 446 190 93 PpuMI rG'GwC_Cy Cuts at: 0 97 268 647 1593 Size: 97 171 379 946 Fragments arranged by size: 946 379 171 97 PspOMI G'GGCC_C Cuts at: 0 156 186 195 1505 1593 Size: 156 30 9 1310 88 Fragments arranged by size: 1310 156 88 30 9 PstI C_TGCA'G Cuts at: 0 645 1034 1145 1593 Size: 645 389 111 448 Fragments arranged by size: 645 448 389 111 PvuII CAG'CTG Cuts at: 0 1029 1347 1593 Size: 1029 318 246 Fragments arranged by size: 1029 318 246 RsrII CG'GwC_CG Cuts at: 0 115 1593 Size: 115 1478 SacII CC_GC'GG Cuts at: 0 95 194 203 1593 Size: 95 99 9 1390 Fragments arranged by size: 1390 99 95 9 SalI G'TCGA_C Cuts at: 0 1183 1593 Size: 1183 410 SanDI GG'GwC_CC Cuts at: 0 97 268 647 1593 Size: 97 171 379 946 Fragments arranged by size: 946 379 171 97 SexAI A'CCwGG_T Cuts at: 0 809 1593 Size: 809 784 SfcI C'TryA_G Cuts at: 0 641 1030 1141 1593 Size: 641 389 111 452 Fragments arranged by size: 641 452 389 111 SfiI GGCCn_nnn'nGGCC Cuts at: 0 194 1593 Size: 194 1399 SfoI GGC'GCC Cuts at: 0 57 1593 Size: 57 1536 SmaI CCC'GGG Cuts at: 0 162 1593 Size: 162 1431 SmlI C'TyrA_G Cuts at: 0 1435 1593 Size: 1435 158 SphI G_CATG'C Cuts at: 0 108 321 1593 Size: 108 213 1272 Fragments arranged by size: 1272 213 108 StyI C'CwwG_G Cuts at: 0 182 248 1538 1593 Size: 182 66 1290 55 Fragments arranged by size: 1290 182 66 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 339 853 1593 Size: 339 514 740 Fragments arranged by size: 740 514 339 TatI w'GTAC_w Cuts at: 0 929 1052 1593 Size: 929 123 541 Fragments arranged by size: 929 541 123 Tth111I GACn'n_nGTC Cuts at: 0 134 1593 Size: 134 1459 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 847 1033 1593 Size: 847 186 560 Fragments arranged by size: 847 560 186 XmaI C'CCGG_G Cuts at: 0 160 1593 Size: 160 1433 Enzymes that do cut: AarI AccI AcuI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI ClaI DraIII DrdI EaeI EarI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII HindIII KasI MslI MspA1I NarI NcoI NspI PasI PfoI PpuMI PspOMI PstI PvuII RsrII SacII SalI SanDI SexAI SfcI SfiI SfoI SmaI SmlI SphI StyI TaqII TatI Tth111I XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BmgBI BmtI BsaAI BsiEI BsiWI BsmI BspEI BspHI BsrGI BssHII BstBI BstEII BstZ17I BtgZI BtsI CspCI DraI EagI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HpaI KpnI MfeI MluI MmeI MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI SacI SapI SbfI ScaI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI TstI XbaI XhoI XmnI ZraI