################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BanII BbeI BbrPI BcgI BclI BfrI BglII BlnI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraII DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI MamI MluI MluNI MroI MscI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SbfI ScaI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Alw44I 23 GTGCAC G^TGCAC AlwNI 1027 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 23 GTGCAC G^TGCAC AspHI 23 GTGCAC G[AT]GC[AT]^C BamHI 529 GGATCC G^GATCC BanI 309 GGCACC G^G[CT][GA]CC BbsI 91 GTCTTC GAAGAC(2/6) BbvCI 533 CCTCAGC CCTCAGC(-5/-2) BbvI 51 GCAGC GCAGC(8/12) BciVI 173 GTATCC GTATCC(6/5) BcnI 1083 CCGGG CC^[GC]GG BfaI 314 CTAG C^TAG BglI 139 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 553 CTGGAG CTGGAG(16/14) BpuAI 91 GTCTTC GAAGAC(2/6) BsaAI 940 TACGTG [CT]AC^GT[GA] BseRI 472 GAGGAG GAGGAG(10/8) BsiEI 879 CGGCCG CG[GA][CT]^CG BsiHKAI 23 GTGCAC G[AT]GC[AT]^C BsmFI 110 GGGAC GGGAC(10/14) BssHII 85 GCGCGC G^CGCGC BstAPI 1100 GCAAATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 432 TTCGAA TT^CGAA BstXI 252 CCACGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 907 TTTAAA TTT^AAA DsaI 140 CCGCGG C^C[GA][CT]GG EagI 879 CGGCCG C^GGCCG EclXI 879 CGGCCG C^GGCCG Eco52I 879 CGGCCG C^GGCCG EcoRI 295 GAATTC G^AATTC EcoT14I 749 CCAAGG C^C[AT][AT]GG HgaI 89 GCGTC GACGC(5/10) KspI 140 CCGCGG CCGC^GG MaeI 314 CTAG C^TAG MaeII 941 ACGT A^CGT MfeI 794 CAATTG C^AATTG MunI 794 CAATTG C^AATTG NaeI 4 GCCGGC GCC^GGC NciI 1083 CCGGG CC^[GC]GG NgoMIV 4 GCCGGC G^CCGGC NlaIII 891 CATG CATG^ NspV 432 TTCGAA TT^CGAA PleI 617 GACTC GAGTC(4/5) SacII 140 CCGCGG CCGC^GG SfuI 432 TTCGAA TT^CGAA StyI 749 CCAAGG C^C[AT][AT]GG TaqI 433 TCGA T^CGA TfiI 1092 GATTC G^A[AT]TC TseI 51 GCAGC G^C[AT]GC Tsp45I 497 GTGAC ^GT[GC]AC TthHB8I 433 TCGA T^CGA XcmI 1004 CCACTGCTGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AfaI 367 GTAC GT^AC 1080 GTAC Asp700I 234 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1018 GAAAAAGTTC AvaII 394 GGACC G^G[AT]CC 663 GGTCC BlpI 831 GCTGAGC GC^T[ACGT]AGC 895 GCTCAGC BmyI 23 GTGCAC G[AGT]GC[ACT]^C 745 GTGCCC Bpu1102I 831 GCTGAGC GC^T[ACGT]AGC 895 GCTCAGC Bsp1286I 23 GTGCAC G[AGT]GC[ACT]^C 745 GTGCCC BspMI 27 ACCTGC ACCTGC(4/8) 1050 GCAGGT BsrFI 4 GCCGGC [GA]^CCGG[CT] 65 GCCGGT BstF5I 390 GGATG GGATG(2/0) 857 GGATG BstYI 78 AGATCC [GA]^GATC[CT] 529 GGATCC CelII 831 GCTGAGC GC^T[ACGT]AGC 895 GCTCAGC CfoI 85 GCGC GCG^C 87 GCGC Cfr10I 4 GCCGGC [GA]^CCGG[CT] 65 GCCGGT Cfr13I 394 GGACC G^G[ACGT]CC 663 GGTCC EaeI 2 TGGCCG [CT]^GGCC[GA] 879 CGGCCG FauI 180 GCGGG CCCGC(4/6) 854 GCGGG FokI 390 GGATG GGATG(9/13) 857 GGATG HaeIII 3 GGCC GG^CC 880 GGCC HhaI 85 GCGC GCG^C 87 GCGC HinP1I 85 GCGC G^CGC 87 GCGC HinfI 617 GACTC G^A[ACGT]TC 1092 GATTC MflI 78 AGATCC [GA]^GATC[CT] 529 GGATCC MseI 403 TTAA T^TAA 908 TTAA MslI 97 CACCACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 253 CACGAAAATG MspA1I 140 CCGCGG C[AC]G^C[GT]G 178 CAGCGG PstI 29 CTGCAG CTGCA^G 1048 CTGCAG RsaI 367 GTAC GT^AC 1080 GTAC SapI 130 GAAGAGC GCTCTTC(1/4) 435 GAAGAGC Sau96I 394 GGACC G^G[ACGT]CC 663 GGTCC SexAI 396 ACCTGGT A^CC[AT]GGT 1031 ACCTGGT SfaNI 810 GATGC GCATC(5/9) 861 GATGC Tru9I 403 TTAA T^TAA 908 TTAA XhoII 78 AGATCC [GA]^GATC[CT] 529 GGATCC XmnI 234 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1018 GAAAAAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AccII AciI AcsI AluI AlwI ApoI BsaJI BsiYI BslI BsrI BssKI BstNI BstUI Cac8I DdeI DpnII EarI EcoRII Fnu4HI HapII HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MspI MvaI MvnI MwoI NdeII NlaIV Sau3AI ScrFI SfcI Tsp509I TspRI